BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0286.0004.p (339 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value pir||I40085 gtcS protein - Bacillus brevis >gi|468746|emb|CAA552... 281 1e-74 ref|NP_781510.1| two-component sensor histidine kinase [Clostrid... 216 8e-55 ref|NP_562108.1| two-component sensor histidine kinase [Clostrid... 164 2e-39 ref|ZP_00099283.1| COG0642: Signal transduction histidine kinase... 145 1e-33 ref|NP_982221.1| sensor histidine kinase [Bacillus cereus ATCC 1... 144 4e-33 >pir||I40085 gtcS protein - Bacillus brevis emb|CAA55265.1| gtcS [Brevibacillus brevis] Length = 370 Score = 281 bits (719), Expect = 1e-74, Method: Compositional matrix adjust. Identities = 139/277 (50%), Positives = 190/277 (68%), Gaps = 5/277 (1%) Query: 29 FGMSIYIFSQLMVKRITRPLEQIAEAIERMGKGEYKERLSITADYEFSVIQHRFNEMAES 88 F +S+Y++S+ KRIT PLE+I +AI++M +GE+ +RL ADYE ++IQ FNEM Sbjct: 53 FALSVYLYSRWTSKRITGPLEKITDAIQKMREGEFAQRLCFKADYELTLIQEHFNEMVAH 112 Query: 89 LEQAERENRRLQDSKQRMLADLSHDLKTPVTTIQGYAKALQLGLVDSEEKKERYLQLIYN 148 LE+ E E +L+ SKQRML DLSHD KTP+TTIQGYA ALQ+G+VDS EK+ RYL++IYN Sbjct: 113 LEKTEAEKNKLEQSKQRMLLDLSHDFKTPITTIQGYAMALQMGVVDSPEKQRRYLEMIYN 172 Query: 149 KATVVTALIDDLFRLSKLERPDHPISVERGDLAELLREIAADYYDAMEDKGMVMELDIPS 208 K+ +VTAL++D+F+L+ L+ PD P + E G EIA Y+D E ++L IP+ Sbjct: 173 KSIIVTALVEDMFQLATLDSPDLPSAEEHGRFGRTGSEIAIAYFDQFEQNKFSLDLKIPT 232 Query: 209 GEVMAGYXXXXDPGLMRRAIANLLSNAVQHNSGGTVVLIALEELAEDVRIRVQDNGGGIS 268 VM + L+ RA++NLLSN ++HN GT V ++L + E + + V DNG GI+ Sbjct: 233 QRVMI----RMNRNLLYRALSNLLSNTLKHNPKGTKVALSLTDTHEAILLEVMDNGIGIA 288 Query: 269 DELKEVIFDPFVRGDAARPGDGGTGLGLAISKQILEL 305 +ELKE IF PFVRGD AR G+ GTGLGL K+ L Sbjct: 289 EELKESIFQPFVRGDKARTGE-GTGLGLPSPKKRLNF 324 >ref|NP_781510.1| two-component sensor histidine kinase [Clostridium tetani E88] gb|AAO35447.1| two-component sensor histidine kinase [Clostridium tetani E88] Length = 480 Score = 216 bits (549), Expect = 8e-55, Method: Compositional matrix adjust. Identities = 111/285 (38%), Positives = 180/285 (63%), Gaps = 5/285 (1%) Query: 29 FGMSIYIFSQLMVKRITRPLEQIAEAIERMGKGEYKERLSITADYEFSVIQHRFNEMAES 88 F + + ++++ K +T PL+++ + ++++ KG+Y R+ + + EF ++ FN M E Sbjct: 179 FALIMIMYAKFTSKNLTMPLKELMKGVKKIIKGDYSTRIYLKSQNEFGDLRDAFNLMVEK 238 Query: 89 LEQAERENRRLQDSKQRMLADLSHDLKTPVTTIQGYAKALQLGLVDSEEKKERYLQLIYN 148 +E+ + + +D+++R++ D+SHDLK P+ +I+GY+ L S ++KE+YL++I N Sbjct: 239 IEEERKLKEKSEDNRKRLMLDISHDLKNPLASIRGYSNYLIENKEISNKEKEKYLKIIEN 298 Query: 149 KATVVTALIDDLFRLSKLERPDHPISVERGDLAELLREIAADYYDAMEDKGMVMELDIPS 208 + V LI DLF LSK E D I E+ D+ EL+REI A++ MEDK MV +P Sbjct: 299 NSIRVNDLITDLFELSKFESIDFSIDFEKMDICELIREIIANHIPQMEDKDMVYSFYLPE 358 Query: 209 GEVMAGYXXXXDPGLMRRAIANLLSNAVQHNSGGTVVLIALEELAEDVRIRVQDNGGGIS 268 EV + + RAI+NLL N++++N GT + ++LEE + V I V DNG GI Sbjct: 359 NEVFI----MGNEKNLYRAISNLLENSIKYNEKGTKIQVSLEEYEDSVGIEVVDNGIGIP 414 Query: 269 DELKEVIFDPFVRGDAARPG-DGGTGLGLAISKQILELHRGKLKL 312 +LK+ IF+PF+R D +R GGTGLGLAI+K I+E H+G++KL Sbjct: 415 KKLKQDIFNPFIRVDTSRNSKQGGTGLGLAITKAIVEKHKGEIKL 459 >ref|NP_562108.1| two-component sensor histidine kinase [Clostridium perfringens] dbj|BAB80898.1| two-component sensor histidine kinase [Clostridium perfringens str. 13] Length = 478 Score = 164 bits (416), Expect = 2e-39, Method: Compositional matrix adjust. Identities = 91/285 (31%), Positives = 167/285 (58%), Gaps = 5/285 (1%) Query: 29 FGMSIYIFSQLMVKRITRPLEQIAEAIERMGKGEYKERLSITADYEFSVIQHRFNEMAES 88 F MS+ ++S+L K T+PL+++ + ++ G+Y R++I + EF + FN MA Sbjct: 181 FTMSLILYSKLTSKTFTKPLDKLMNGVRKLSLGDYSTRINIEKNNEFEELGEAFNNMASK 240 Query: 89 LEQAERENRRLQDSKQRMLADLSHDLKTPVTTIQGYAKALQLGLVDSEEKKERYLQLIYN 148 +E+ ++ + + ++ + +++HDLKTP+++I GY+ ++ +E +Y+ +I N Sbjct: 241 IEEEKKLKEKSEKLRKEFVRNIAHDLKTPLSSIIGYSNIIKNNPDIEKESLHKYISIIEN 300 Query: 149 KATVVTALIDDLFRLSKLERPDHPISVERGDLAELLREIAADYYDAMEDKGMVMELDIPS 208 +I +LF L+ + + + DL+E LR + ADY +E+K + +I Sbjct: 301 NGERANEMIMELFEFYTLQSSEFILHKKYQDLSEFLRNLIADYIYLLEEKDFDFDFEISE 360 Query: 209 GEVMAGYXXXXDPGLMRRAIANLLSNAVQHNSGGTVVLIALEELAEDVRIRVQDNGGGIS 268 ++ + D + RAI NL+ N+V++N GT L++L++ + V+I V D+G G+S Sbjct: 361 DDLYLEF----DNKRLERAIGNLIINSVKYNKKGTKFLVSLKKEEDKVKIIVSDDGIGLS 416 Query: 269 DELKEVIFDPFVRGDAARPG-DGGTGLGLAISKQILELHRGKLKL 312 +E+K+ IF+PFVR + +R GG+GLGL ISK+I+E H G + L Sbjct: 417 EEIKKHIFNPFVREEKSRNSKKGGSGLGLTISKEIVEKHGGTIYL 461 >ref|ZP_00099283.1| COG0642: Signal transduction histidine kinase [Desulfitobacterium hafniense] Length = 389 Score = 145 bits (366), Expect = 1e-33, Method: Compositional matrix adjust. Identities = 88/277 (31%), Positives = 151/277 (54%), Gaps = 5/277 (1%) Query: 36 FSQLMVKRITRPLEQIAEAIERMGKGEYKERLSITADYEFSVIQHRFNEMAESLEQAERE 95 F + + KR+ +P+EQ+ + + + KG Y+ + + EF+ + + FNEMA L+++ER Sbjct: 94 FFRRLEKRVFKPMEQLKQGFDEIAKGNYQVSIEPEVENEFTQLIYAFNEMAFKLQESERS 153 Query: 96 NRRLQDSKQRMLADLSHDLKTPVTTIQGYAKALQLGLVDSEEKKERYLQLIYNKATVVTA 155 N+ + +++ ++A++SHDLKTP+T+IQGY + + G V EK +RYL+ IY+ + Sbjct: 154 NQEYEKNRKTLVANISHDLKTPITSIQGYLEVILEGCVGEPEKIKRYLETIYSNTAYMNK 213 Query: 156 LIDDLFRLSKLERPDHPISVERGDLAELLREIAADYYDAMEDKGMVMELDIPSGEVMAGY 215 LIDDLF SKL+ ++ E + L ++A + E++ Y Sbjct: 214 LIDDLFLFSKLDLDKVAMNYEVLSIGAYLDDLATELKFEFEERQFKF---FYQNRTDRDY 270 Query: 216 XXXXDPGLMRRAIANLLSNAVQHNSGGTV-VLIALEELAEDVRIRVQDNGGGISDELKEV 274 D + + I N++ NA+++ + V + L +D+ I + DNG GI +E Sbjct: 271 AVTIDGKRVYQGIRNIIDNAMKYGPEQDLKVTMELYVQEDDICIDIADNGPGIPEEQLPH 330 Query: 275 IFDPFVRGDAARPGD-GGTGLGLAISKQILELHRGKL 310 IF+ F R D R D TGLGLAI+K+++E G + Sbjct: 331 IFNRFYRLDTERSKDFTSTGLGLAIAKELIEAQGGTI 367 >ref|NP_982221.1| sensor histidine kinase [Bacillus cereus ATCC 10987] gb|AAS45063.1| sensor histidine kinase [Bacillus cereus ATCC 10987] Length = 455 Score = 144 bits (362), Expect = 4e-33, Method: Compositional matrix adjust. Identities = 91/291 (31%), Positives = 162/291 (55%), Gaps = 15/291 (5%) Query: 39 LMVKRITRPLEQIAEAIERMGKGEYKERLSITADYEFSVIQHRFNEMAESLEQAERENRR 98 L+ + +T+PL ++ +A E M KG+ L+I E + H ++A+ L ++E Sbjct: 178 LLSRFLTKPLIRMKKATETMSKGDLSVSLNINGKDEVGELAHSIQKLADDLNYMKKE--- 234 Query: 99 LQDSKQRMLADLSHDLKTPVTTIQGYAKALQLGLVDSEEKKERYLQLIYNKATVVTALID 158 + LA ++H+L+TP+T I+GYA + + ++++K YL +I +A VT L+ Sbjct: 235 ----RGEFLASVAHELRTPLTYIRGYADIILKTKMSTQDQK-HYLSIIKEEADHVTGLVQ 289 Query: 159 DLFRLSKLERPDHPISVERGDLAELLREIAADYYDAMEDKGMVMELDIPSGEVMAGYXXX 218 DLF L+K+E+ + I+ ++ +L +LL+ I A A +K + + P V+ Sbjct: 290 DLFELAKMEKHNFIINTKQTNLYDLLKHIVAKIQPAYTEKNIHIIFSCPEDLVL-----L 344 Query: 219 XDPGLMRRAIANLLSNAVQHNSGGTVVLIALEELAEDVRIRVQDNGGGISDELKEVIFDP 278 D + I NLL+NA H+ + + + + + E ++I V+D G GI ++ IF+ Sbjct: 345 LDEQRFEQVILNLLNNAYSHSEVNSKIFVMVTDKKESIQIIVKDEGEGIPEKDLPHIFER 404 Query: 279 FVRGDAARP-GDGGTGLGLAISKQILELHRGKLKLDNRNGL-TVFELVVRK 327 F R D +R GG+GLGLAI K+I+ELHRG + + + G T+F ++++K Sbjct: 405 FYRVDKSRTRATGGSGLGLAIVKEIIELHRGSISVKSELGHGTIFTILLKK 455 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.137 0.373 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 453,036,783 Number of extensions: 18283482 Number of successful extensions: 75460 Number of sequences better than 1.0e-05: 4736 Number of HSP's gapped: 67586 Number of HSP's successfully gapped: 4744 Length of query: 339 Length of database: 757,237,578 Length adjustment: 126 Effective length of query: 213 Effective length of database: 415,135,104 Effective search space: 88423777152 Effective search space used: 88423777152 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 126 (53.1 bits)