BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0284.0026.p (275 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00061282.1| COG3693: Beta-1,4-xylanase [Clostridium therm... 186 4e-46 pdb|1JJF|A Chain A, Structural Basis For The Substrate Specifici... 186 6e-46 pdb|1JT2|A Chain A, Structural Basis For The Substrate Specifici... 184 1e-45 ref|ZP_00061170.1| COG2382: Enterochelin esterase and related en... 179 8e-44 ref|NP_839203.1| putative xylanase [Shigella flexneri 2a str. 24... 108 1e-22 >ref|ZP_00061282.1| COG3693: Beta-1,4-xylanase [Clostridium thermocellum ATCC 27405] sp|P10478|XYNZ_CLOTM Endo-1,4-beta-xylanase Z precursor (Xylanase Z) (1,4-beta-D-xylan xylanohydrolase Z) pir||A31842 endo-1,4-beta-xylanase (EC 3.2.1.8) Z precursor - Clostridium thermocellum gb|AAA23286.1| xylanase Z Length = 837 Score = 186 bits (473), Expect = 4e-46, Method: Compositional matrix adjust. Identities = 96/185 (51%), Positives = 119/185 (64%), Gaps = 11/185 (5%) Query: 14 PAGYDKERENIARGMIGTIEYPSSTVGNTRKAMVYTPPGYSAGQEYSVLYLLHGIGGDET 73 P+GYD+ R + RG + I Y S+ +TR A VY PPGYS ++YSVLYLLHGIGG E Sbjct: 36 PSGYDQVRNGVPRGQVVNISYFSTATNSTRPARVYLPPGYSKDKKYSVLYLLHGIGGSEN 95 Query: 74 EWYNHG-QPQIILDNLYADEMLKPMIVVLPNGRAMLNDRAEGDIFAPDKVQAFTTFEADL 132 +W+ G + +I DNL A+ +KP+I+V PN A P + F DL Sbjct: 96 DWFEGGGRANVIADNLIAEGKIKPLIIVTPNTNAA----------GPGIADGYENFTKDL 145 Query: 133 LHDLIPYIEANYPVLTDRTHRALAGLSMGGGQSLNIGLNNLDRFAWIGAFSPAPNTKLPE 192 L+ LIPYIE+NY V TDR HRA+AGLSMGGGQS NIGL NLD+FA+IG S APNT E Sbjct: 146 LNSLIPYIESNYSVYTDREHRAIAGLSMGGGQSFNIGLTNLDKFAYIGPISAAPNTYPNE 205 Query: 193 LLVPE 197 L P+ Sbjct: 206 RLFPD 210 >pdb|1JJF|A Chain A, Structural Basis For The Substrate Specificity Of The Feruloyl Esterase Domain Of The Cellulosomal Xylanase Z Of Clostridium Thermocellum Length = 268 Score = 186 bits (472), Expect = 6e-46, Method: Compositional matrix adjust. Identities = 96/191 (50%), Positives = 121/191 (63%), Gaps = 11/191 (5%) Query: 14 PAGYDKERENIARGMIGTIEYPSSTVGNTRKAMVYTPPGYSAGQEYSVLYLLHGIGGDET 73 P+GYD+ R + RG + I Y S+ +TR A VY PPGYS ++YSVLYLLHGIGG E Sbjct: 17 PSGYDQVRNGVPRGQVVNISYFSTATNSTRPARVYLPPGYSKDKKYSVLYLLHGIGGSEN 76 Query: 74 EWYNHG-QPQIILDNLYADEMLKPMIVVLPNGRAMLNDRAEGDIFAPDKVQAFTTFEADL 132 +W+ G + +I DNL A+ +KP+I+V PN A P + F DL Sbjct: 77 DWFEGGGRANVIADNLIAEGKIKPLIIVTPNTNAA----------GPGIADGYENFTKDL 126 Query: 133 LHDLIPYIEANYPVLTDRTHRALAGLSMGGGQSLNIGLNNLDRFAWIGAFSPAPNTKLPE 192 L+ LIPYIE+NY V TDR HRA+AGLSMGGGQS NIGL NLD+FA+IG S APNT E Sbjct: 127 LNSLIPYIESNYSVYTDREHRAIAGLSMGGGQSFNIGLTNLDKFAYIGPISAAPNTYPNE 186 Query: 193 LLVPEPQRPQK 203 L P+ + + Sbjct: 187 RLFPDGGKAAR 197 >pdb|1JT2|A Chain A, Structural Basis For The Substrate Specificity Of The Ferul Domain Of The Cellulosomal Xylanase Z From C. Thermocellum Length = 268 Score = 184 bits (468), Expect = 1e-45, Method: Compositional matrix adjust. Identities = 95/191 (49%), Positives = 121/191 (63%), Gaps = 11/191 (5%) Query: 14 PAGYDKERENIARGMIGTIEYPSSTVGNTRKAMVYTPPGYSAGQEYSVLYLLHGIGGDET 73 P+GYD+ R + RG + I Y S+ +TR A VY PPGYS ++YSVLYLLHGIGG E Sbjct: 17 PSGYDQVRNGVPRGQVVNISYFSTATNSTRPARVYLPPGYSKDKKYSVLYLLHGIGGSEN 76 Query: 74 EWYNHG-QPQIILDNLYADEMLKPMIVVLPNGRAMLNDRAEGDIFAPDKVQAFTTFEADL 132 +W+ G + +I DNL A+ +KP+I+V PN A P + F DL Sbjct: 77 DWFEGGGRANVIADNLIAEGKIKPLIIVTPNTNAA----------GPGIADGYENFTKDL 126 Query: 133 LHDLIPYIEANYPVLTDRTHRALAGLSMGGGQSLNIGLNNLDRFAWIGAFSPAPNTKLPE 192 L+ LIPYIE+NY V TDR HRA+AGL+MGGGQS NIGL NLD+FA+IG S APNT E Sbjct: 127 LNSLIPYIESNYSVYTDREHRAIAGLAMGGGQSFNIGLTNLDKFAYIGPISAAPNTYPNE 186 Query: 193 LLVPEPQRPQK 203 L P+ + + Sbjct: 187 RLFPDGGKAAR 197 >ref|ZP_00061170.1| COG2382: Enterochelin esterase and related enzymes [Clostridium thermocellum ATCC 27405] Length = 501 Score = 179 bits (453), Expect = 8e-44, Method: Compositional matrix adjust. Identities = 95/186 (51%), Positives = 115/186 (61%), Gaps = 12/186 (6%) Query: 14 PAGYDKERENIARGMIGTIEYPSSTVGNTRKAMVYTPPGYSAGQEYSVLYLLHGIGGDET 73 P+GYD+ R I RG + I Y S+ TR A VY PPGYS + YSVLYLLHGIGG E Sbjct: 36 PSGYDQVRGGIQRGQVVNISYYSTATNGTRPAKVYLPPGYSTSKRYSVLYLLHGIGGSEG 95 Query: 74 EWYNH--GQPQIILDNLYADEMLKPMIVVLPNGRAMLNDRAEGDIFAPDKVQAFTTFEAD 131 +W+ G+ II DNL A+ +KP+I+V PN A P + F D Sbjct: 96 DWFADWGGRASIIADNLIAEGKIKPLIIVTPNTNAA----------GPGIGDGYENFTKD 145 Query: 132 LLHDLIPYIEANYPVLTDRTHRALAGLSMGGGQSLNIGLNNLDRFAWIGAFSPAPNTKLP 191 L++ LIPYIE+ Y V TDR HRA+AGLSMGGGQS NIGL NLD+FA+IG S APNT Sbjct: 146 LINCLIPYIESRYSVYTDREHRAIAGLSMGGGQSFNIGLTNLDKFAYIGPISSAPNTYPN 205 Query: 192 ELLVPE 197 L P+ Sbjct: 206 NRLFPD 211 >ref|NP_839203.1| putative xylanase [Shigella flexneri 2a str. 2457T] gb|AAP19014.1| putative xylanase [Shigella flexneri 2a str. 2457T] Length = 391 Score = 108 bits (270), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 66/192 (34%), Positives = 99/192 (51%), Gaps = 7/192 (3%) Query: 7 NYKMQTAPAGYDKERENIARGMIGTIEYPSSTVGNTRKAMVYTPPGYSA-GQEYSVLYLL 65 N M P Y R ++A G + I Y S+ + + R+ V+TPPGY+ G+ VLY Sbjct: 122 NSSMILVPGSYLDTR-SVAHGDLIAITYHSNALQSERQMYVWTPPGYTGMGEPLPVLYFY 180 Query: 66 HGIGGDETEWYNHGQPQIILDNLYADEMLKPMIVVLPNGRAMLNDRAEGDIFAPDKVQAF 125 HG G + G+ I+DNL A+ +KPM+VV+P+ D ++ + F Sbjct: 181 HGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPDTETDAKGIIPEDFVPQERRKVF 240 Query: 126 TTFEA-----DLLHDLIPYIEANYPVLTDRTHRALAGLSMGGGQSLNIGLNNLDRFAWIG 180 A +L++D+IP I + V D RALAGLS GG Q+L G+N+L+ F W+ Sbjct: 241 YPLNAKAADRELMNDIIPLISKRFSVRKDADGRALAGLSQGGYQALVSGMNHLESFGWLA 300 Query: 181 AFSPAPNTKLPE 192 FS T +P+ Sbjct: 301 TFSGVTTTTVPD 312 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.135 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 459,348,926 Number of extensions: 19938519 Number of successful extensions: 38129 Number of sequences better than 1.0e-05: 47 Number of HSP's gapped: 38105 Number of HSP's successfully gapped: 47 Length of query: 275 Length of database: 757,237,578 Length adjustment: 124 Effective length of query: 151 Effective length of database: 420,565,302 Effective search space: 63505360602 Effective search space used: 63505360602 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 125 (52.8 bits)