BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0282.0004.p (386 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value pir||S71439 phosphoserine transaminase (EC 2.6.1.52) - Bacillus ... 588 e-167 pdb|1BT4|A Chain A, Phosphoserine Aminotransferase From Bacillus... 586 e-166 sp|Q59196|SERC_BACCI Phosphoserine aminotransferase (PSAT) >gi|2... 585 e-166 gb|AAF13453.1| phosphoserine aminotransferase [Bacillus alcaloph... 443 e-123 ref|NP_242054.1| phosphoserine aminotransferase [Bacillus halodu... 437 e-121 >pir||S71439 phosphoserine transaminase (EC 2.6.1.52) - Bacillus circulans Length = 362 Score = 588 bits (1516), Expect = e-167, Method: Compositional matrix adjust. Identities = 271/362 (74%), Positives = 318/362 (87%) Query: 25 LSKRAYNFNAGPAALPLTVLERAQAEFVDFRESGMSIMEMSHRGAIYESVHNEAQERLLS 84 +SKRAYNFNAGPAALPL VLERAQAEFVD++ +GMSIMEMSHRGA+YE+VHNEAQ RLL+ Sbjct: 1 MSKRAYNFNAGPAALPLEVLERAQAEFVDYQHTGMSIMEMSHRGAVYEAVHNEAQARLLA 60 Query: 85 LLGNPQGYKVLFIQGGASTQFAMIPMNFVTEGKVGSYVMTGSWAEKAFKEAKLVGGAHAA 144 LLGNP GYKVLFIQGGASTQFAMIPMNF+ EG+ +YVMTGSWA KA KEAKL+G H A Sbjct: 61 LLGNPTGYKVLFIQGGASTQFAMIPMNFLKEGQTANYVMTGSWASKALKEAKLIGDTHVA 120 Query: 145 ASSEDKKFLAIPELSSIKAADNAAYLHVTSNETIEGTQFAEYPDAGSIPLIADMSSDILS 204 ASSE ++ +P+L I+ DNAAYLH+TSNETIEG QF +PD GS+PLI DMSSDILS Sbjct: 121 ASSEASNYMTLPKLQEIQLQDNAAYLHLTSNETIEGAQFKAFPDTGSVPLIGDMSSDILS 180 Query: 205 RSFDVNQFELIYAGAQKNLGPSGVTVVIVKEELIAKSPSNIPTILRYDTHLKNNSLYNTP 264 R FD+NQF L+YAGAQKNLGPSGVTVVIV+E+L+A+SP ++PT+LRYDT++KNNSLYNTP Sbjct: 181 RPFDLNQFGLVYAGAQKNLGPSGVTVVIVREDLVAESPKHLPTMLRYDTYVKNNSLYNTP 240 Query: 265 PSFSVYMVNEVLKWIEEQGGLAGIEVKNRDKAALLYDHIDGSGGFYRGVAEAGSRSIMNV 324 PSF +YMVNEVLKWIEE+GGL G++ NR KA+L+YD ID SGGFYRG + SRS MN+ Sbjct: 241 PSFGIYMVNEVLKWIEERGGLEGVQQANRKKASLIYDAIDQSGGFYRGCVDVDSRSDMNI 300 Query: 325 TFRMQSEELEKQFVKAAEQEGFVGLKGHRSVGGLRASIYNAVPYESIKALSDFMKHFQQT 384 TFR+ SEELEK+FVKA+EQEGFVGLKGHRSVGGLRASIYNAVPYES +AL FM+HF+++ Sbjct: 301 TFRLASEELEKEFVKASEQEGFVGLKGHRSVGGLRASIYNAVPYESCEALVQFMEHFKRS 360 Query: 385 QG 386 +G Sbjct: 361 RG 362 >pdb|1BT4|A Chain A, Phosphoserine Aminotransferase From Bacillus Circulans Subsp. Alkalophilus Length = 362 Score = 586 bits (1511), Expect = e-166, Method: Compositional matrix adjust. Identities = 270/362 (74%), Positives = 318/362 (87%) Query: 25 LSKRAYNFNAGPAALPLTVLERAQAEFVDFRESGMSIMEMSHRGAIYESVHNEAQERLLS 84 +S+RAYNFNAGPAALPL VLERAQAEFVD++ +GMSIMEMSHRGA+YE+VHNEAQ RLL+ Sbjct: 1 MSERAYNFNAGPAALPLEVLERAQAEFVDYQHTGMSIMEMSHRGAVYEAVHNEAQARLLA 60 Query: 85 LLGNPQGYKVLFIQGGASTQFAMIPMNFVTEGKVGSYVMTGSWAEKAFKEAKLVGGAHAA 144 LLGNP GYKVLFIQGGASTQFAMIPMNF+ EG+ +YVMTGSWA KA KEAKL+G H A Sbjct: 61 LLGNPTGYKVLFIQGGASTQFAMIPMNFLKEGQTANYVMTGSWASKALKEAKLIGDTHVA 120 Query: 145 ASSEDKKFLAIPELSSIKAADNAAYLHVTSNETIEGTQFAEYPDAGSIPLIADMSSDILS 204 ASSE ++ +P+L I+ DNAAYLH+TSNETIEG QF +PD GS+PLI DMSSDILS Sbjct: 121 ASSEASNYMTLPKLQEIQLQDNAAYLHLTSNETIEGAQFKAFPDTGSVPLIGDMSSDILS 180 Query: 205 RSFDVNQFELIYAGAQKNLGPSGVTVVIVKEELIAKSPSNIPTILRYDTHLKNNSLYNTP 264 R FD+NQF L+YAGAQKNLGPSGVTVVIV+E+L+A+SP ++PT+LRYDT++KNNSLYNTP Sbjct: 181 RPFDLNQFGLVYAGAQKNLGPSGVTVVIVREDLVAESPKHLPTMLRYDTYVKNNSLYNTP 240 Query: 265 PSFSVYMVNEVLKWIEEQGGLAGIEVKNRDKAALLYDHIDGSGGFYRGVAEAGSRSIMNV 324 PSF +YMVNEVLKWIEE+GGL G++ NR KA+L+YD ID SGGFYRG + SRS MN+ Sbjct: 241 PSFGIYMVNEVLKWIEERGGLEGVQQANRKKASLIYDAIDQSGGFYRGCVDVDSRSDMNI 300 Query: 325 TFRMQSEELEKQFVKAAEQEGFVGLKGHRSVGGLRASIYNAVPYESIKALSDFMKHFQQT 384 TFR+ SEELEK+FVKA+EQEGFVGLKGHRSVGGLRASIYNAVPYES +AL FM+HF+++ Sbjct: 301 TFRLASEELEKEFVKASEQEGFVGLKGHRSVGGLRASIYNAVPYESCEALVQFMEHFKRS 360 Query: 385 QG 386 +G Sbjct: 361 RG 362 >sp|Q59196|SERC_BACCI Phosphoserine aminotransferase (PSAT) emb|CAA86558.1| phosphoserine aminotransferase [Bacillus circulans subsp. alkalophilus] Length = 362 Score = 585 bits (1508), Expect = e-166, Method: Compositional matrix adjust. Identities = 267/362 (73%), Positives = 318/362 (87%) Query: 25 LSKRAYNFNAGPAALPLTVLERAQAEFVDFRESGMSIMEMSHRGAIYESVHNEAQERLLS 84 +SKRAYNFNAGPAALPL VLERAQAEFVD++ +GMSIMEMSHRGA+YE+VHNEAQ R+L+ Sbjct: 1 MSKRAYNFNAGPAALPLEVLERAQAEFVDYQHTGMSIMEMSHRGAVYEAVHNEAQARMLA 60 Query: 85 LLGNPQGYKVLFIQGGASTQFAMIPMNFVTEGKVGSYVMTGSWAEKAFKEAKLVGGAHAA 144 L+GNP GYKVLFIQGGASTQFAMIPMNF+ EG+ +YVMTGSWA KA KEAKL+G H A Sbjct: 61 LMGNPTGYKVLFIQGGASTQFAMIPMNFLKEGQTANYVMTGSWASKALKEAKLIGDTHVA 120 Query: 145 ASSEDKKFLAIPELSSIKAADNAAYLHVTSNETIEGTQFAEYPDAGSIPLIADMSSDILS 204 ASSE ++ +P+L I+ DNAAYLH+TSNETIEG QF +PD GS+PLI DMSSDI+S Sbjct: 121 ASSEASNYMTMPKLQEIQLQDNAAYLHLTSNETIEGAQFKAFPDTGSVPLIGDMSSDIMS 180 Query: 205 RSFDVNQFELIYAGAQKNLGPSGVTVVIVKEELIAKSPSNIPTILRYDTHLKNNSLYNTP 264 R FD+NQF L+YAGAQKNLGPSGVTVVIV+E+L+A+SP ++PT+LRYDT++KNNS+YNTP Sbjct: 181 RPFDLNQFGLVYAGAQKNLGPSGVTVVIVREDLVAESPKHLPTMLRYDTYVKNNSMYNTP 240 Query: 265 PSFSVYMVNEVLKWIEEQGGLAGIEVKNRDKAALLYDHIDGSGGFYRGVAEAGSRSIMNV 324 PSF +YMVNEVLKWIEE+GGL G++ NR KA+L+YD ID SGGFYRG + SRS MN+ Sbjct: 241 PSFGIYMVNEVLKWIEERGGLEGVQQANRKKASLIYDAIDQSGGFYRGCVDVDSRSDMNI 300 Query: 325 TFRMQSEELEKQFVKAAEQEGFVGLKGHRSVGGLRASIYNAVPYESIKALSDFMKHFQQT 384 TFR+ SEELEK+FVKA+EQEGFVGLKGHRSVGGLRASIYNAVPYES +AL FM+HF+++ Sbjct: 301 TFRLASEELEKEFVKASEQEGFVGLKGHRSVGGLRASIYNAVPYESCEALVQFMEHFKRS 360 Query: 385 QG 386 +G Sbjct: 361 RG 362 >gb|AAF13453.1| phosphoserine aminotransferase [Bacillus alcalophilus] Length = 361 Score = 443 bits (1140), Expect = e-123, Method: Compositional matrix adjust. Identities = 204/360 (56%), Positives = 270/360 (75%) Query: 25 LSKRAYNFNAGPAALPLTVLERAQAEFVDFRESGMSIMEMSHRGAIYESVHNEAQERLLS 84 + K+ +NFNAGP+ALP LERAQ E ++F ++ MS+ME+SHR YE VH +AQ L Sbjct: 1 MVKQVFNFNAGPSALPKPALERAQKELLNFNDTQMSVMELSHRSQSYEEVHEQAQNLLRE 60 Query: 85 LLGNPQGYKVLFIQGGASTQFAMIPMNFVTEGKVGSYVMTGSWAEKAFKEAKLVGGAHAA 144 LL P Y++LF+QGGAS QF M+PMN +T+G +G+YV+TGSW+EKA KEAKL+G H A Sbjct: 61 LLQIPNDYQILFLQGGASLQFTMLPMNLLTKGTIGNYVLTGSWSEKALKEAKLLGETHIA 120 Query: 145 ASSEDKKFLAIPELSSIKAADNAAYLHVTSNETIEGTQFAEYPDAGSIPLIADMSSDILS 204 AS++ + +IP+ S + +N AYLH+TSN TI GTQ+ +P+ PLIADMSSDILS Sbjct: 121 ASTKANSYQSIPDFSEFQLNENDAYLHITSNNTIYGTQYQNFPEINHAPLIADMSSDILS 180 Query: 205 RSFDVNQFELIYAGAQKNLGPSGVTVVIVKEELIAKSPSNIPTILRYDTHLKNNSLYNTP 264 R VNQF +IYAGAQKNLGPSGVTVVIVK++L+ +PT+L+Y TH+K++SLYNTP Sbjct: 181 RPLKVNQFGMIYAGAQKNLGPSGVTVVIVKKDLLNTKVEQVPTMLQYATHIKSDSLYNTP 240 Query: 265 PSFSVYMVNEVLKWIEEQGGLAGIEVKNRDKAALLYDHIDGSGGFYRGVAEAGSRSIMNV 324 P+FS+YM+ VL WI++ GG I +N +KA ++YD ID S GFY G AE GSRS+MNV Sbjct: 241 PTFSIYMLRNVLDWIKDLGGAEAIAKQNEEKAKIIYDTIDESNGFYVGHAEKGSRSLMNV 300 Query: 325 TFRMQSEELEKQFVKAAEQEGFVGLKGHRSVGGLRASIYNAVPYESIKALSDFMKHFQQT 384 TF +++EEL +QF+ A+++GFVGL GHRSVGG RASIYNAVP ++ AL + M F++ Sbjct: 301 TFNLRNEELNQQFLAKAKEQGFVGLNGHRSVGGCRASIYNAVPIDACIALRELMIQFKEN 360 >ref|NP_242054.1| phosphoserine aminotransferase [Bacillus halodurans] sp|Q9KDM4|SERC_BACHD Phosphoserine aminotransferase (PSAT) pir||D83798 phosphoserine aminotransferase serC [imported] - Bacillus halodurans (strain C-125) dbj|BAB04907.1| phosphoserine aminotransferase [Bacillus halodurans] Length = 361 Score = 437 bits (1125), Expect = e-121, Method: Compositional matrix adjust. Identities = 204/359 (56%), Positives = 269/359 (74%) Query: 27 KRAYNFNAGPAALPLTVLERAQAEFVDFRESGMSIMEMSHRGAIYESVHNEAQERLLSLL 86 KRAYNFNAGP+ALP VLE+AQ+E +DF +GMS+ME+SHR YE+VH+ A + L LL Sbjct: 2 KRAYNFNAGPSALPTEVLEKAQSELLDFENTGMSVMELSHRSKEYENVHHTAAQLLRDLL 61 Query: 87 GNPQGYKVLFIQGGASTQFAMIPMNFVTEGKVGSYVMTGSWAEKAFKEAKLVGGAHAAAS 146 P+ Y VLF+QGGAS QFAMIP+NF+ EGKV +Y++TGSW+EKA KEAK +G A S Sbjct: 62 NIPEDYDVLFLQGGASLQFAMIPLNFLDEGKVANYILTGSWSEKALKEAKFIGKTAIAGS 121 Query: 147 SEDKKFLAIPELSSIKAADNAAYLHVTSNETIEGTQFAEYPDAGSIPLIADMSSDILSRS 206 +++ + IP++SS++ ++ +Y+H+TSN TI GTQ+ YP PLI DMSSDILSR Sbjct: 122 TKESNYTFIPDISSLQYNEHDSYVHLTSNNTIFGTQWHTYPSVSHAPLIVDMSSDILSRP 181 Query: 207 FDVNQFELIYAGAQKNLGPSGVTVVIVKEELIAKSPSNIPTILRYDTHLKNNSLYNTPPS 266 V F+LIYAGAQKNLGPSGVTVVI+++EL+ ++ ++PT+LRY TH + SLYNTPP+ Sbjct: 182 LPVKNFDLIYAGAQKNLGPSGVTVVIIRKELLKRNVDHVPTMLRYQTHAEKQSLYNTPPT 241 Query: 267 FSVYMVNEVLKWIEEQGGLAGIEVKNRDKAALLYDHIDGSGGFYRGVAEAGSRSIMNVTF 326 F +YM+ EVL+W++ GG I +N+ KA L+Y ID S FY+G A SRS+MNVTF Sbjct: 242 FGIYMLKEVLQWLKNIGGTEQIAERNQTKANLIYGAIDESEQFYKGHATKESRSLMNVTF 301 Query: 327 RMQSEELEKQFVKAAEQEGFVGLKGHRSVGGLRASIYNAVPYESIKALSDFMKHFQQTQ 385 + +EEL +QF+ A+++GFVGL GHRSVGG RASIYN VP E+ +AL+DFM F QT Sbjct: 302 TLPTEELTQQFLSEAKEKGFVGLNGHRSVGGCRASIYNGVPVEACEALADFMHSFYQTH 360 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.316 0.132 0.374 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 533,512,058 Number of extensions: 21289607 Number of successful extensions: 56434 Number of sequences better than 1.0e-05: 354 Number of HSP's gapped: 55844 Number of HSP's successfully gapped: 360 Length of query: 386 Length of database: 757,237,578 Length adjustment: 127 Effective length of query: 259 Effective length of database: 412,420,005 Effective search space: 106816781295 Effective search space used: 106816781295 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 127 (53.5 bits)