BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0281.0005.p (185 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_924256.1| hypothetical protein gll1310 [Gloeobacter viola... 122 4e-27 ref|NP_486098.1| unknown protein [Nostoc sp. PCC 7120] >gi|25531... 114 1e-24 gb|EAI09893.1| unknown [environmental sequence] 111 1e-23 gb|EAF27884.1| unknown [environmental sequence] 110 2e-23 ref|ZP_00079612.1| COG0596: Predicted hydrolases or acyltransfer... 95 9e-19 >ref|NP_924256.1| hypothetical protein gll1310 [Gloeobacter violaceus] dbj|BAC89251.1| gll1310 [Gloeobacter violaceus PCC 7421] Length = 341 Score = 122 bits (306), Expect = 4e-27, Method: Compositional matrix adjust. Identities = 68/162 (41%), Positives = 97/162 (59%), Gaps = 7/162 (4%) Query: 6 GEFTNIGSHKL--FRMIEGGDERYLPVVFEHGCGSSALLWSLVAPEIARITTTIVYDRAG 63 G ++G H+L + GG V+ E G G+ +L W V E+A+ T T+ YDRAG Sbjct: 44 GRLVDVGGHRLHLLEVGTGGPT----VILEAGGGAWSLEWCRVQAEVAKFTRTVSYDRAG 99 Query: 64 YGWSELGPFPRTNERCISELYELLQIEGIKGPYILVGHSYGGLNVRMFTHKHPELVAGVV 123 YGWS+ G T+ + EL+ LL+ I GPYILVG S GGL++RMF H +P+ VAGVV Sbjct: 100 YGWSDAGLKNPTSRQVSEELHALLEKAEIAGPYILVGRSLGGLHMRMFAHLYPQEVAGVV 159 Query: 124 LVDSMHEDEMTARFPEEHRKGQLFAVKFFWFLQVLSKIGILK 165 LVD+ HEDE P+ G+ + W L++L+ IG+++ Sbjct: 160 LVDASHEDENRRILPQLRAAGERNLNRLGW-LRLLAPIGLIR 200 >ref|NP_486098.1| unknown protein [Nostoc sp. PCC 7120] pir||AD2063 hypothetical protein all2058 [imported] - Nostoc sp. (strain PCC 7120) dbj|BAB73757.1| ORF_ID:all2058~unknown protein [Nostoc sp. PCC 7120] Length = 331 Score = 114 bits (284), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 68/168 (40%), Positives = 100/168 (59%), Gaps = 5/168 (2%) Query: 2 QIDRGEFTNIGSHKLFRMIEGGDERYLPVVFEHGCGSSALLWSLVAPEIARITTTIVYDR 61 Q G+ ++G ++L R+ G R VV EH G + L+ EIA++T +YDR Sbjct: 32 QSPPGKLIDVGGYRL-RLYTAGHSRP-TVVLEHSLG--GIEGYLLVEEIAKLTRVCIYDR 87 Query: 62 AGYGWSELGPFPRTNERCISELYELLQIEGIKGPYILVGHSYGGLNVRMFTHKHPELVAG 121 AGYGWS+ P PRT+ + ++EL LL I GI+ PYILVG+S+G NVR++ H P+ V G Sbjct: 88 AGYGWSDNSPHPRTSRQIVAELDFLLTIAGIEPPYILVGNSFGSYNVRLYAHYFPDKVLG 147 Query: 122 VVLVDSMHEDEMTARFPEEHRKGQLFAVKFFWFLQVLSKIGILKLLAV 169 +VL D +HE M + + +LF V F+ + S +GI++LL V Sbjct: 148 IVLTDGLHETGML-KMSIYLQALKLFFVSGFFMSILASILGIIRLLNV 194 >gb|EAI09893.1| unknown [environmental sequence] Length = 296 Score = 111 bits (277), Expect = 1e-23, Method: Compositional matrix adjust. Identities = 51/131 (38%), Positives = 82/131 (62%), Gaps = 2/131 (1%) Query: 3 IDRGEFTNIGSHKLFRMIEGGDERYLPVVFEHGCGSSALLWSLVAPEIARITTTIVYDRA 62 I GE ++G + L+ E + V+F+ G G +W V+ E+++ I YDRA Sbjct: 24 IKTGEMIDVGGYSLY--TEDSGMGRVNVIFDSGMGDDLSVWKKVSDEVSKFARVITYDRA 81 Query: 63 GYGWSELGPFPRTNERCISELYELLQIEGIKGPYILVGHSYGGLNVRMFTHKHPELVAGV 122 G GWS+ P RT+E + EL+ +L+ + + GP ILVGHS+GG+N++ F K+P+ +AG+ Sbjct: 82 GLGWSDESPKERTSEEIVRELHSMLEKKKLIGPIILVGHSFGGVNMQQFALKYPDEIAGL 141 Query: 123 VLVDSMHEDEM 133 VL+DS HE ++ Sbjct: 142 VLIDSAHESQI 152 >gb|EAF27884.1| unknown [environmental sequence] Length = 266 Score = 110 bits (274), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 57/157 (36%), Positives = 93/157 (59%), Gaps = 7/157 (4%) Query: 10 NIGSHKLFRMIEGGDERYLPVVFEHGCGSSALLWSLVAPEIARITTTIVYDRAGYGWSEL 69 +IG + L+ +E + V+F+ G G + +W+ VA E+++ + I YDRAG GWSE Sbjct: 2 DIGDYSLY--VEDIGTGKVTVIFDAGMGDDSSVWNKVAEEVSQFSRVITYDRAGLGWSEE 59 Query: 70 GPFPRTNERCISELYELLQIEGIKGPYILVGHSYGGLNVRMFTHKHPELVAGVVLVDSMH 129 P R ++ ++EL+ +L+ + I GP ILVGHS+GG+N++ + +PE +A +VLVDS H Sbjct: 60 SPHDRDSKVIVAELHSVLKDKDITGPIILVGHSFGGVNMQRYALTYPEYIAALVLVDSAH 119 Query: 130 EDEMTARFPEEHRKGQLFAVKFFWFLQVLSKIGILKL 166 ED++ EE + F F + +GI +L Sbjct: 120 EDQI-----EEMPQTNYLQKYLFKFGMWAAPVGIPRL 151 >ref|ZP_00079612.1| COG0596: Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily) [Geobacter metallireducens] Length = 346 Score = 94.7 bits (234), Expect = 9e-19, Method: Compositional matrix adjust. Identities = 52/148 (35%), Positives = 77/148 (52%), Gaps = 3/148 (2%) Query: 24 DERYLP-VVFEHGCGSSALLWSLVAPEIARITTTIVYDRAGYGWSELGPFPRTNERCISE 82 D R P VV E G G + W + PE+A+ + YDRAG GWS PRT Sbjct: 83 DRRGAPTVVLETGLGGMSSAWGWIQPEVAKFARVVSYDRAGLGWSAPDDLPRTALTVTRR 142 Query: 83 LYELLQIEGIKGPYILVGHSYGGLNVRMFTHKHPELVAGVVLVDSMHEDEMTARFPEEHR 142 L LL ++GPY+LVGHS GG+ +R F ++P VAG+VL+D+ H D+ R R Sbjct: 143 LRALLHDGEVEGPYVLVGHSMGGMFMRFFADQYPGDVAGMVLIDAAHPDQH--RRSPAIR 200 Query: 143 KGQLFAVKFFWFLQVLSKIGILKLLAVF 170 + + + +L+ +G +++ F Sbjct: 201 RHMSSGFRMLRGVPLLAGLGYVRMTGFF 228 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.326 0.144 0.445 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 278,517,193 Number of extensions: 11071805 Number of successful extensions: 33070 Number of sequences better than 1.0e-05: 52 Number of HSP's gapped: 33432 Number of HSP's successfully gapped: 52 Length of query: 185 Length of database: 757,237,578 Length adjustment: 118 Effective length of query: 67 Effective length of database: 436,855,896 Effective search space: 29269345032 Effective search space used: 29269345032 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 122 (51.6 bits)