BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0281.0002.p (481 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value pir||S10789 amylase A-180 - alkaliphilic eubacterium 163-26 >gi|... 412 e-114 dbj|BAA09831.1| chitinase [Kurthia zopfii] 125 2e-27 gb|AAF74782.1| chitinase [Bacillus circulans] 125 2e-27 sp|P21543|AMYB_PAEPO Beta/alpha-amylase precursor [Includes: Bet... 124 6e-27 emb|CAA68344.1| precursor polypeptide [synthetic construct] 124 7e-27 >pir||S10789 amylase A-180 - alkaliphilic eubacterium 163-26 emb|CAA37453.1| precursor polypeptide (AA -37 to 1647) [unidentified bacterium] Length = 1684 Score = 412 bits (1060), Expect = e-114, Method: Compositional matrix adjust. Identities = 233/474 (49%), Positives = 290/474 (61%), Gaps = 42/474 (8%) Query: 1 VTAGKPGSPNPGNKVTVYYKEGWTNVNIHYRAEGGTWTSAPGVKMENDPSNPGYKKITVD 60 + GKPG+P GNKVT+YY+ G+ +HYR EGG WT+APG+KME D Y ++T+D Sbjct: 1069 IIPGKPGAPIDGNKVTIYYQNGFDTPYVHYRPEGGNWTNAPGLKME-DSEFASYSRLTLD 1127 Query: 61 VGTASRLEACFNNGSIVWDSNGQKNYFFNTGDNIYIPGNNNAPGQVKLGEKPA------- 113 +G A+R E FNNG +WDS+ + NYFFN GDN YIPG N + G++ G KP Sbjct: 1128 IGEANRAEVAFNNGRGLWDSDNENNYFFNIGDNTYIPGKNGSAGEI-YGGKPRPPLVGNE 1186 Query: 114 -------GSDTTAPSIPANLAGNLSSGTP-----KTVTLTWSASSDNVGVAD-----YQI 156 G DT P + G + P K+ +S + ++G AD + Sbjct: 1187 VIIYYKNGFDT--PYVHYRPEGGTWTNAPGIKMDKSEIAGYSKITLDIGRADRVEVAFND 1244 Query: 157 TRKAGTSTET----FTVKATTYIDSTVKEGTTYKYQVRARDAANNFSANSNEVTIEVPGA 212 R A S F V TY K+ V D + IE P Sbjct: 1245 GRGAWDSDNERNYLFVVGNNTYEPGINGAPGQVKHGVLPDDG-------EDPGDIEDP-- 1295 Query: 213 GDIIAPSAPTNVKGTATSASVALQWTASTDNVAVAGYEIYRGAVKVGTSTTPAFTDTGLA 272 D +PS PT++ AT+ +V+L WTAS D+V VAGY+IYR V++G + + +TD+GL Sbjct: 1296 -DHTSPSKPTDLTAIATAHTVSLSWTASADDVEVAGYKIYRDGVEIGVTESTTYTDSGLT 1354 Query: 273 EETPYTYTVKXXXXXXXXXXXXXELTVVTLKGTIVIPGGDKPYSTNPSFGKRVSTPITID 332 ET Y+Y V+ ELT+ T + T V PGGD PYSTNPSFGK+V+TPITID Sbjct: 1355 AETTYSYMVQAYDTSNNFSALSDELTIETAEKTGVDPGGDMPYSTNPSFGKKVTTPITID 1414 Query: 333 GTNSGEWTDDMLIAIDMAGDDPRTLGSNWSLHETPMDLSHLWAAWDNEFLYLAWQYVDVT 392 G N GEWTDDMLIAI MAGDDPR+LG NWS+HETPMDL+HLW AWD+E+LYLAWQYVDVT Sbjct: 1415 GVNDGEWTDDMLIAIGMAGDDPRSLGDNWSMHETPMDLTHLWGAWDHEYLYLAWQYVDVT 1474 Query: 393 DIVDPANAGSAGGTPIRSMDMPQTIAIDTISGAGATHDMWKKNGNQPLWGGLDL 446 DI+DPANAGS+ GT I MDMPQTIAIDTI GATHDMW KNG + LWGG DL Sbjct: 1475 DIIDPANAGSSAGTTISQMDMPQTIAIDTIPEQGATHDMWGKNGGESLWGGPDL 1528 Score = 94.4 bits (233), Expect = 6e-18, Method: Compositional matrix adjust. Identities = 44/88 (50%), Positives = 58/88 (65%), Gaps = 1/88 (1%) Query: 12 GNKVTVYYKEGWTNVNIHYRAEGGTWTSAPGVKMENDPSNPGYKKITVDVGTASRLEACF 71 GN VT+YYK+G+ +HYR EGG WT PG++ME + GY K+TVD+ AS+LE F Sbjct: 977 GNLVTIYYKKGFDTPYMHYRPEGGEWTIVPGIRME-ESEIAGYSKLTVDIREASKLEVAF 1035 Query: 72 NNGSIVWDSNGQKNYFFNTGDNIYIPGN 99 NNG WDS+ + NY F G + YIP + Sbjct: 1036 NNGRGAWDSDQENNYLFEPGVHTYIPSH 1063 Score = 58.9 bits (141), Expect = 3e-07, Method: Compositional matrix adjust. Identities = 52/165 (31%), Positives = 70/165 (42%), Gaps = 8/165 (4%) Query: 120 PSIPANLAGNLSSGTPKTVTLTWSASSDNVGVADYQITRKAGTSTETFTVKATTYIDSTV 179 P PA NLS T+T+ + V +Y+I R T TT+ D + Sbjct: 792 PKEPATTPKNLSVVNVTETTVTFEWDQSDGYVVEYEILRDEDVVASTIR---TTFTDEDL 848 Query: 180 KEGTTYKYQVRARDAANNFSANSNEVTIEVPGAGDIIAP--SAPTNVK-GTATSASVALQ 236 TTY Y V A SA S + + D AP N++ T +V + Sbjct: 849 NPDTTYTYSVVAVGEGGQKSAPSEALKVTTLEENDEPKEPAEAPENLRIADITDTTVTIN 908 Query: 237 WTASTDNVAVAGYEIYRGAVKVGTSTTPAFTDTGLAEETPYTYTV 281 W AS N V GYE+ R V +G +T F DTGL + YTYT+ Sbjct: 909 WNAS--NGYVTGYEVLRDGVVIGETTRTTFIDTGLDADRTYTYTI 951 >dbj|BAA09831.1| chitinase [Kurthia zopfii] Length = 699 Score = 125 bits (315), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 94/237 (39%), Positives = 126/237 (53%), Gaps = 22/237 (9%) Query: 74 GSIVWDSNGQKNYFFNTGDNIYIPGNNNAPGQVKLGEKPAGSDTTAPSIPANLAGNLSSG 133 G++ W+ +G +N +P P PA DTTAPS+P N S+G Sbjct: 429 GAMFWELSGDRNKTLQNKLKADLPTGGTVP--------PA--DTTAPSVPGNA---RSTG 475 Query: 134 -TPKTVTLTWSASSDNVGVADYQITRKAGTSTETFTVKATTYIDSTVKEGTTYKYQVRAR 192 T +VTL W+AS+DNVGV Y + A + +V TT S + GT+Y + ++A+ Sbjct: 476 VTVNSVTLAWNASTDNVGVTGYNVYNGANLAA---SVNGTTATISGLSAGTSYTFTIKAK 532 Query: 193 DAANNFSANSNEVTIEV---PGAGDIIAPSAPTNVKGTA-TSASVALQWTASTDNVAVAG 248 DAA N SA SN VT+ PG GD AP+APTN+ TA T++S+ L W ASTDNV V G Sbjct: 533 DAAGNLSAASNAVTVSTTTQPG-GDTQAPTAPTNLASTAQTTSSITLSWAASTDNVGVTG 591 Query: 249 YEIYRGAVKVGTSTTPAFTDTGLAEETPYTYTVKXXXXXXXXXXXXXELTVVTLKGT 305 Y++Y G + T T +GLA +T YT+TVK LTV T GT Sbjct: 592 YDVYNGTALATSVTGTTATISGLAADTSYTFTVKAKDAAGNSSVASNALTVKTAAGT 648 >gb|AAF74782.1| chitinase [Bacillus circulans] Length = 717 Score = 125 bits (314), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 95/236 (40%), Positives = 126/236 (53%), Gaps = 20/236 (8%) Query: 74 GSIVWDSNGQKNYFFNTGDNIYIPGNNNAPGQVKLGEKPAGSDTTAPSIPANLAGNLSSG 133 G++ W+ +G +N N + G +DTTAPS+P N S+G Sbjct: 429 GAMFWELSGDRNKTLQ----------NKLKSDLSTGGTVPPTDTTAPSVPGNAR---STG 475 Query: 134 -TPKTVTLTWSASSDNVGVADYQITRKAGTSTETFTVKATTYIDSTVKEGTTYKYQVRAR 192 T +VTL W+AS+DNVGV Y + GTS T +V TT S + GT+Y + V+A+ Sbjct: 476 VTASSVTLAWNASTDNVGVTGYTVYN--GTSLVT-SVTGTTATISGLAPGTSYTFTVKAK 532 Query: 193 DAANNFSANSNEVTIE--VPGAGDIIAPSAPTNVKGTA-TSASVALQWTASTDNVAVAGY 249 DAA N SA SN +T+ V GD AP+ PTN+ TA TS+++ L W ASTDNV V GY Sbjct: 533 DAAGNLSAASNSLTVSTTVQPGGDTQAPTVPTNLTSTAKTSSTITLSWAASTDNVGVIGY 592 Query: 250 EIYRGAVKVGTSTTPAFTDTGLAEETPYTYTVKXXXXXXXXXXXXXELTVVTLKGT 305 E+Y G V T + + T TGL +T YT+TVK LTV T GT Sbjct: 593 EVYNGTALVTTVSGTSATVTGLTADTSYTFTVKAKDAAGNLSAASSALTVKTEVGT 648 >sp|P21543|AMYB_PAEPO Beta/alpha-amylase precursor [Includes: Beta-amylase ; Alpha-amylase ] pir||A29130 beta-amylase (EC 3.2.1.2) / alpha-amylase (EC 3.2.1.1) precursor - Bacillus polymyxa gb|AAA85446.1| beta-amylase Length = 1196 Score = 124 bits (310), Expect = 6e-27, Method: Compositional matrix adjust. Identities = 75/160 (46%), Positives = 100/160 (62%), Gaps = 10/160 (6%) Query: 1 VTAGKPGSPNPG------NKVTVYYKEGWTNVNIHYRAEGGTWTSAPGVKMENDPSNPGY 54 +T+G P NPG NKVTVYYK+G+ + IHYR GG+WT+APGVKM+ D GY Sbjct: 551 ITSGAPAGANPGDGGGTTNKVTVYYKKGFNSPYIHYRPAGGSWTAAPGVKMQ-DAEISGY 609 Query: 55 KKITVDVGTASRLEACFNNGSIVWDSNGQKNYFFNTGDNIYIPGNNNAPGQVKLGEKPAG 114 KITVD+G+AS+LEA FN+G+ WDSN KNY F+TG + Y PG+N A G ++ G P+G Sbjct: 610 AKITVDIGSASQLEAAFNDGNNNWDSNNTKNYLFSTGTSTYTPGSNGAAGTIRTGA-PSG 668 Query: 115 SDTTAPSIPANLAGNLSSGTPKTVTLTWSASSDNVGVADY 154 S + + + A +L+ T T S S NV + Y Sbjct: 669 SVLSV--VTSTYATDLNEVTGPIQTEKLSGVSLNVSTSTY 706 Score = 113 bits (283), Expect = 1e-23, Method: Compositional matrix adjust. Identities = 59/105 (56%), Positives = 77/105 (73%), Gaps = 2/105 (1%) Query: 12 GNKVTVYYKEGWTNVNIHYRAEGGTWTSAPGVKMENDPSNPGYKKITVDVGTASRLEACF 71 GNKVT+YYK+G+ + IHYR GG+WT+APGVKM+ D GY KITVD+G+AS+LEA F Sbjct: 458 GNKVTIYYKKGFNSPYIHYRPAGGSWTAAPGVKMQ-DAEISGYAKITVDIGSASQLEAAF 516 Query: 72 NNGSIVWDSNGQKNYFFNTGDNIYIPGNNNAPGQVKLGEKPAGSD 116 N+G+ WDSN KNY F+TG + Y PGN+ G + G PAG++ Sbjct: 517 NDGNNNWDSNNTKNYSFSTGTSTYTPGNSGNAGTITSGA-PAGAN 560 >emb|CAA68344.1| precursor polypeptide [synthetic construct] Length = 779 Score = 124 bits (310), Expect = 7e-27, Method: Compositional matrix adjust. Identities = 66/121 (54%), Positives = 85/121 (70%), Gaps = 8/121 (6%) Query: 1 VTAGKPGSPNPG------NKVTVYYKEGWTNVNIHYRAEGGTWTSAPGVKMENDPSNPGY 54 +T+G P NPG NKVTVYYK+G+ + IHYR GG+WT+APGVKM+ D GY Sbjct: 554 ITSGAPAGTNPGDGGGTTNKVTVYYKKGFNSPYIHYRPAGGSWTAAPGVKMQ-DAEISGY 612 Query: 55 KKITVDVGTASRLEACFNNGSIVWDSNGQKNYFFNTGDNIYIPGNNNAPGQVKLGEKPAG 114 KITVD+G+AS+LEA FN+G+ WDSN KNY F+TG + Y PG+N A G ++ G P+G Sbjct: 613 AKITVDIGSASQLEAAFNDGNNNWDSNNTKNYLFSTGTSTYTPGSNGAAGTIRTGT-PSG 671 Query: 115 S 115 S Sbjct: 672 S 672 Score = 112 bits (280), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 58/105 (55%), Positives = 76/105 (72%), Gaps = 2/105 (1%) Query: 12 GNKVTVYYKEGWTNVNIHYRAEGGTWTSAPGVKMENDPSNPGYKKITVDVGTASRLEACF 71 GNKVT+YYK+G+ + IHYR GG+WT+APGVKM+ GY KITVD+G+AS+LEA F Sbjct: 461 GNKVTIYYKKGFNSPYIHYRPAGGSWTAAPGVKMQ-AAEISGYAKITVDIGSASQLEAAF 519 Query: 72 NNGSIVWDSNGQKNYFFNTGDNIYIPGNNNAPGQVKLGEKPAGSD 116 N+G+ WDSN KNY F+TG + Y PGN+ G + G PAG++ Sbjct: 520 NDGNNNWDSNNTKNYLFSTGTSTYTPGNSGNAGTITSGA-PAGTN 563 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.312 0.130 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 806,779,108 Number of extensions: 36769366 Number of successful extensions: 67844 Number of sequences better than 1.0e-05: 101 Number of HSP's gapped: 67755 Number of HSP's successfully gapped: 176 Length of query: 481 Length of database: 757,237,578 Length adjustment: 129 Effective length of query: 352 Effective length of database: 406,989,807 Effective search space: 143260412064 Effective search space used: 143260412064 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 128 (53.9 bits)