BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0280.0026.p (562 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_831391.1| Zn-dependent hydrolase [Bacillus cereus ATCC 14... 669 0.0 ref|NP_243264.1| BH2398~unknown conserved protein [Bacillus halo... 667 0.0 ref|NP_978051.1| metallo-beta-lactamase family protein [Bacillus... 665 0.0 ref|NP_622873.1| predicted hydrolase of the metallo-beta-lactama... 658 0.0 ref|NP_844087.1| metallo-beta-lactamase family protein [Bacillus... 652 0.0 >ref|NP_831391.1| Zn-dependent hydrolase [Bacillus cereus ATCC 14579] gb|AAP08592.1| Zn-dependent hydrolase [Bacillus cereus ATCC 14579] Length = 557 Score = 669 bits (1727), Expect = 0.0, Method: Compositional matrix adjust. Identities = 327/560 (58%), Positives = 422/560 (75%), Gaps = 8/560 (1%) Query: 4 KNNNDKLTIFALGGVGEIGKNMYVVQYGNDIVVVDAGLKFPEEDMLGIDIVIPDISYLTE 63 KNN L+I ALGGV EIGKNMY +QY NDIVV+D G KFP+E +LGID++IPD++YL E Sbjct: 5 KNN---LSIVALGGVNEIGKNMYAIQYENDIVVIDCGSKFPDESLLGIDLIIPDVTYLQE 61 Query: 64 NRDKVRGIVLTHGHEDHIGGLSYVLKNLNVPVYGTRLTLGLVENKLKEANLLGETKRILI 123 N++K+RG+++THGHEDHIGG+ Y LK LNVP+Y T+LTLGL+E KLKE NL +T+ I+I Sbjct: 62 NKEKIRGLIVTHGHEDHIGGIPYFLKQLNVPIYATKLTLGLIEIKLKEHNLQNDTELIVI 121 Query: 124 NEDSEIELGSSLKVTFFRTNHSIPDSVGVCIETPEGNVVHTGDFKFDHTPVNGQFANLHR 183 + +SEI+ GS +K TFF+TNHSIPD +G+ TPEG VVHTGDFKFD TPVN Q ++H+ Sbjct: 122 HSESEIDCGS-IKATFFKTNHSIPDCLGIVFHTPEGTVVHTGDFKFDLTPVNNQHPDIHK 180 Query: 184 MAEIGQRGVLALMSDSTNAEKPGFTPSEKNVGIVLEDIFRKAEQRVVVATFASNVHRIQQ 243 MA+IG GVLAL+S+STNAE+PGFTPSE++VG +E+IF KA ++VV++TFASNV+R+QQ Sbjct: 181 MAKIGSEGVLALLSESTNAERPGFTPSERSVGERIEEIFMKANRKVVISTFASNVNRVQQ 240 Query: 244 VVNAAESTGRKITVIGRSMVNVVSIASELGYLHVPDGMLIEPEEMNRMAGNRVVVLCXXX 303 +V A T RK+ ++GRSMVNVV +A E GYLH+P+GMLIE E+NR+ +V +LC Sbjct: 241 IVEACIKTNRKLALLGRSMVNVVEVALEKGYLHIPEGMLIEANEVNRLDPKQVAILC--- 297 Query: 304 XGSQGEPMSALTRMARSSHRKVDILPGDTVIIAATPVPGNEKYVGRTIDELFRLGANVIY 363 GSQGEPM+AL R+A ++R+VDILP DTVIIAATP+PGNE+ V R ID LF LGA VIY Sbjct: 298 TGSQGEPMAALARLASGNYRQVDILPEDTVIIAATPIPGNERNVSRIIDNLFALGAKVIY 357 Query: 364 -XXXXXXXXXXXXXXQEELKLMLNLMKPKYFLPIHGEFRMQRRHALLAESVGVEPANIFI 422 QEELKLML LMKPKYF+PIHGEFRM H+LLAES+G+E NIFI Sbjct: 358 GSGSSTGVHVSGHAYQEELKLMLTLMKPKYFIPIHGEFRMLHHHSLLAESIGIEKENIFI 417 Query: 423 TELGEVIEISGGSARRAGKVTAXXXXXXXXXXXXXXXXXLRDRKLLSQDGILVVVVTLSK 482 G+V++IS A ++ ++ A LRDRK LS+DG+LV+V+T +K Sbjct: 418 VRNGDVVDISNEVAIQSRRIQAGNIYVDGLGIGDVGNALLRDRKQLSEDGMLVIVITFNK 477 Query: 483 QNGAIVSGPDIISRGFVYVRESEGLLDEANRIVSGTLQRLMSEKVNEWASLKTSVKDSLG 542 +G I+SGPDIISRGFVYVR+SE L E N++ T+ L E VN W LK V+++LG Sbjct: 478 VDGEIISGPDIISRGFVYVRDSEEFLRELNKLAVITINNLKKENVNSWGILKREVREALG 537 Query: 543 RFLYEQTRRRPMILPIIMEV 562 +++Y T+R+PMILPII+EV Sbjct: 538 KYIYTNTKRKPMILPIIIEV 557 >ref|NP_243264.1| BH2398~unknown conserved protein [Bacillus halodurans] pir||F83949 hypothetical protein BH2398 [imported] - Bacillus halodurans (strain C-125) dbj|BAB06117.1| BH2398~unknown conserved protein [Bacillus halodurans] Length = 555 Score = 667 bits (1721), Expect = 0.0, Method: Compositional matrix adjust. Identities = 328/559 (58%), Positives = 424/559 (75%), Gaps = 6/559 (1%) Query: 4 KNNNDKLTIFALGGVGEIGKNMYVVQYGNDIVVVDAGLKFPEEDMLGIDIVIPDISYLTE 63 K + + +FALGGVGEIGKNMYVV+ +D+ V+DAGL FP+++MLG+D+VIPDISYL E Sbjct: 3 KQTTESIRVFALGGVGEIGKNMYVVEVDDDLFVIDAGLMFPDDEMLGVDVVIPDISYLVE 62 Query: 64 NRDKVRGIVLTHGHEDHIGGLSYVLKNLNVPVYGTRLTLGLVENKLKEANLLGETKRILI 123 N ++VR I+LTHGHEDHIGGL YVL+ LNVPVYGT+LTLGLVE KLKEA L+ K LI Sbjct: 63 NEERVRAILLTHGHEDHIGGLPYVLQKLNVPVYGTKLTLGLVEEKLKEAGLIRSAKLKLI 122 Query: 124 NEDSEIELGSSLKVTFFRTNHSIPDSVGVCIETPEGNVVHTGDFKFDHTPVNGQFANLHR 183 + +S ++LGS+ V+FFRTNHSIPDSVG+CI+T +G +VHTGDFKFD TPV+G+ A + + Sbjct: 123 DSNSRLKLGST-PVSFFRTNHSIPDSVGICIQTSQGFIVHTGDFKFDQTPVDGKQAEIGK 181 Query: 184 MAEIGQRGVLALMSDSTNAEKPGFTPSEKNVGIVLEDIFRKAEQRVVVATFASNVHRIQQ 243 MA IG +GVL L+SDSTNAE+PG T SE VG + + F + + R++V TFASNVHR+QQ Sbjct: 182 MAAIGHKGVLCLLSDSTNAERPGMTKSETEVGRGIAEAFEQTKGRIIVTTFASNVHRVQQ 241 Query: 244 VVNAAESTGRKITVIGRSMVNVVSIASELGYLHVPDGMLIEPEEMNRMAGNRVVVLCXXX 303 V++AA +T RK+ V GRSMV VVSIA LGYL PD + I+ EE+++ RV ++ Sbjct: 242 VIHAAIATNRKLAVAGRSMVKVVSIAERLGYLEAPDDLFIDIEEVSKYDDERVAIIT--- 298 Query: 304 XGSQGEPMSALTRMARSSHRKVDILPGDTVIIAATPVPGNEKYVGRTIDELFRLGANVIY 363 GSQGEPMSAL+RMA+ +HR++ I DTVIIAATP+PGNE+ V +D L R+GA+VI+ Sbjct: 299 TGSQGEPMSALSRMAKGAHRQITITENDTVIIAATPIPGNERSVSTIVDLLHRIGADVIF 358 Query: 364 XXXXXXXXXXXXXXQEELKLMLNLMKPKYFLPIHGEFRMQRRHALLAESVGVEPANIFIT 423 EELKLMLNLM+PK+F+PIHGEFRMQ H LA+SVG+ IF+ Sbjct: 359 GHGKVHASGHGSA--EELKLMLNLMRPKFFVPIHGEFRMQHAHKELAKSVGIREEAIFLV 416 Query: 424 ELGEVIEISGGSARRAGKVTAXXXXXXXXXXXXXXXXXLRDRKLLSQDGILVVVVTLSKQ 483 + GEV+E G R+AGKV + LRDR+LLS+DGILVVVVTL+KQ Sbjct: 417 DKGEVVEFRNGQGRKAGKVPSGNVLIDGLGVGDVGNIVLRDRRLLSKDGILVVVVTLNKQ 476 Query: 484 NGAIVSGPDIISRGFVYVRESEGLLDEANRIVSGTLQRLMSEKVNEWASLKTSVKDSLGR 543 +G I+SGP+IISRGFVYVRESE L++EAN +V+ TL++ ++E VNEW+SLK++V++ L R Sbjct: 477 SGTILSGPNIISRGFVYVRESEKLIEEANELVTETLKKCVTENVNEWSSLKSNVREVLSR 536 Query: 544 FLYEQTRRRPMILPIIMEV 562 FL+E+T+RRPMILPIIMEV Sbjct: 537 FLFEKTKRRPMILPIIMEV 555 >ref|NP_978051.1| metallo-beta-lactamase family protein [Bacillus cereus ATCC 10987] gb|AAS40659.1| metallo-beta-lactamase family protein [Bacillus cereus ATCC 10987] Length = 557 Score = 665 bits (1715), Expect = 0.0, Method: Compositional matrix adjust. Identities = 325/562 (57%), Positives = 423/562 (75%), Gaps = 8/562 (1%) Query: 2 SKKNNNDKLTIFALGGVGEIGKNMYVVQYGNDIVVVDAGLKFPEEDMLGIDIVIPDISYL 61 S KNN ++I ALGGV EIGKNMYV+Q+ NDIVV+D G KFP+E +LGID++IPD++YL Sbjct: 3 SLKNN---VSIVALGGVNEIGKNMYVIQFENDIVVIDCGSKFPDESLLGIDLIIPDVTYL 59 Query: 62 TENRDKVRGIVLTHGHEDHIGGLSYVLKNLNVPVYGTRLTLGLVENKLKEANLLGETKRI 121 EN++K+RG+V+THGHEDHIGG+ Y LK LNVP+Y T+LTLGL+E KLKE NL T+ I Sbjct: 60 QENKEKIRGLVVTHGHEDHIGGIPYFLKQLNVPIYATKLTLGLIEIKLKEHNLQDNTELI 119 Query: 122 LINEDSEIELGSSLKVTFFRTNHSIPDSVGVCIETPEGNVVHTGDFKFDHTPVNGQFANL 181 +I+ +SEI+ G+ +K TFF+TNHSIPD +G+ TPEG +VHTGDFKFD TPVN Q ++ Sbjct: 120 VIHSESEIDFGA-IKTTFFKTNHSIPDCLGIVFHTPEGTIVHTGDFKFDLTPVNNQHPDI 178 Query: 182 HRMAEIGQRGVLALMSDSTNAEKPGFTPSEKNVGIVLEDIFRKAEQRVVVATFASNVHRI 241 H+MA+IG GVLAL+S+STNAE+PGFTPSE++VG +E+IF KA ++V+++TFASNV+R+ Sbjct: 179 HKMAKIGSEGVLALLSESTNAERPGFTPSERSVGERIEEIFMKANRKVIISTFASNVNRV 238 Query: 242 QQVVNAAESTGRKITVIGRSMVNVVSIASELGYLHVPDGMLIEPEEMNRMAGNRVVVLCX 301 QQ+V A T RK+ ++GRSMVNVV +A E GYL++PDGMLIE E+N + +V +LC Sbjct: 239 QQIVEACIKTNRKLALLGRSMVNVVEVALEKGYLNIPDGMLIEANEVNNVDPKQVAILC- 297 Query: 302 XXXGSQGEPMSALTRMARSSHRKVDILPGDTVIIAATPVPGNEKYVGRTIDELFRLGANV 361 GSQGEPM+AL R+A ++R+VD+LP DTVIIAATP+PGNE+ V R ID LF LGA V Sbjct: 298 --TGSQGEPMAALARLASGNYRQVDVLPEDTVIIAATPIPGNERNVSRIIDNLFALGARV 355 Query: 362 IY-XXXXXXXXXXXXXXQEELKLMLNLMKPKYFLPIHGEFRMQRRHALLAESVGVEPANI 420 IY QEELKLML LMKPKYF+PIHGEFRM H+LLAES+GVE NI Sbjct: 356 IYGSGSSTGVHVSGHAYQEELKLMLTLMKPKYFIPIHGEFRMLHHHSLLAESIGVEKENI 415 Query: 421 FITELGEVIEISGGSARRAGKVTAXXXXXXXXXXXXXXXXXLRDRKLLSQDGILVVVVTL 480 FI G+V++IS A ++ K+ A LRDRK LS+DG+LV+V+T Sbjct: 416 FIVRNGDVVDISNQVAIQSRKIQAGNIYVDGLGIGDVGNALLRDRKQLSEDGMLVIVITF 475 Query: 481 SKQNGAIVSGPDIISRGFVYVRESEGLLDEANRIVSGTLQRLMSEKVNEWASLKTSVKDS 540 +K +G I+SGPDIISRGFVYVR+SE L E N++ T+ + EKVN W LK V+++ Sbjct: 476 NKVDGEIISGPDIISRGFVYVRDSEEFLKELNKLAVITINNVKKEKVNSWGILKREVREA 535 Query: 541 LGRFLYEQTRRRPMILPIIMEV 562 LG+++Y T+R+PMILPII+EV Sbjct: 536 LGKYIYTSTKRKPMILPIIIEV 557 >ref|NP_622873.1| predicted hydrolase of the metallo-beta-lactamase superfamily [Thermoanaerobacter tengcongensis] gb|AAM24477.1| predicted hydrolase of the metallo-beta-lactamase superfamily [Thermoanaerobacter tengcongensis] Length = 554 Score = 658 bits (1698), Expect = 0.0, Method: Compositional matrix adjust. Identities = 315/554 (56%), Positives = 419/554 (75%), Gaps = 5/554 (0%) Query: 9 KLTIFALGGVGEIGKNMYVVQYGNDIVVVDAGLKFPEEDMLGIDIVIPDISYLTENRDKV 68 KL I LGG+ EIGKNM V +YGNDI+VVD GL FP+++MLGID+VIPDI+YL +NR+KV Sbjct: 6 KLKIIPLGGLNEIGKNMTVFEYGNDIIVVDCGLAFPDDEMLGIDLVIPDITYLLKNREKV 65 Query: 69 RGIVLTHGHEDHIGGLSYVLKNLNVPVYGTRLTLGLVENKLKEANLLGETKRILINEDSE 128 + IVLTHGHEDHIG L YVLK LNVP+YGTRLT+GLVE KLKE LL ++K I + Sbjct: 66 KAIVLTHGHEDHIGALPYVLKQLNVPIYGTRLTIGLVEYKLKEHGLLRDSKLITVKPRET 125 Query: 129 IELGSSLKVTFFRTNHSIPDSVGVCIETPEGNVVHTGDFKFDHTPVNGQFANLHRMAEIG 188 +E+G KV F RT+HSI DS + I TP G V H+GDFK D TP++G+ +LHR A++G Sbjct: 126 VEIGQ-FKVEFIRTSHSIADSAALAIHTPVGTVFHSGDFKIDFTPIDGEVPDLHRFAQLG 184 Query: 189 QRGVLALMSDSTNAEKPGFTPSEKNVGIVLEDIFRKAEQRVVVATFASNVHRIQQVVNAA 248 ++GVL ++ DSTN E+PG+T SE+ VG +++FRKAEQR++VATFASN+HR+QQ++N+A Sbjct: 185 EKGVLVMLCDSTNIERPGYTMSERTVGETFDNLFRKAEQRIIVATFASNIHRVQQIINSA 244 Query: 249 ESTGRKITVIGRSMVNVVSIASELGYLHVPDGMLIEPEEMNRMAGNRVVVLCXXXXGSQG 308 + GRK+ + GRSMVNVV++A ELGYL VP+G+LI+ +E N++ N+VV++ GSQG Sbjct: 245 QKYGRKVAISGRSMVNVVNVAMELGYLTVPEGLLIDLDEANKLPHNKVVIIT---TGSQG 301 Query: 309 EPMSALTRMARSSHRKVDILPGDTVIIAATPVPGNEKYVGRTIDELFRLGANVIYXXXXX 368 EPMSALTRMA S H+KV+I+PGDTVII+A+P+PGNE+ + R I++LF+ GANVIY Sbjct: 302 EPMSALTRMASSEHKKVEIVPGDTVIISASPIPGNERLISRVINQLFKKGANVIY-EALA 360 Query: 369 XXXXXXXXXQEELKLMLNLMKPKYFLPIHGEFRMQRRHALLAESVGVEPANIFITELGEV 428 QEE+KL+ L+KPK+F+P+HGE+R +HA LAES+G+ P NIF+ + G V Sbjct: 361 DIHVSGHACQEEIKLLHTLIKPKFFIPVHGEYRHLVQHAKLAESLGMPPENIFVVDNGTV 420 Query: 429 IEISGGSARRAGKVTAXXXXXXXXXXXXXXXXXLRDRKLLSQDGILVVVVTLSKQNGAIV 488 +E + S R AG VTA LRDRK L+QDG+LVVVVT+SK+ GA++ Sbjct: 421 LEFTKDSGRIAGTVTAGKVLVDGLGVGDVGNIVLRDRKHLAQDGLLVVVVTISKETGAVI 480 Query: 489 SGPDIISRGFVYVRESEGLLDEANRIVSGTLQRLMSEKVNEWASLKTSVKDSLGRFLYEQ 548 +GPDIISRGFVYVRESE L++E+ IV L + E++NEWA++K+ +KD+L FLYE+ Sbjct: 481 AGPDIISRGFVYVRESEDLMEESKNIVREVLAKCEKEEINEWATIKSMIKDNLSNFLYEK 540 Query: 549 TRRRPMILPIIMEV 562 T+R PMILPIIME+ Sbjct: 541 TKRNPMILPIIMEI 554 >ref|NP_844087.1| metallo-beta-lactamase family protein [Bacillus anthracis str. Ames] gb|AAP25573.1| metallo-beta-lactamase family protein [Bacillus anthracis str. Ames] Length = 549 Score = 652 bits (1682), Expect = 0.0, Method: Compositional matrix adjust. Identities = 321/551 (58%), Positives = 413/551 (74%), Gaps = 8/551 (1%) Query: 2 SKKNNNDKLTIFALGGVGEIGKNMYVVQYGNDIVVVDAGLKFPEEDMLGIDIVIPDISYL 61 S KNN L+I ALGGV EIGKNMY +QY NDIVV+D G KFP+E +LGID++IPD++YL Sbjct: 3 SLKNN---LSIVALGGVNEIGKNMYAIQYENDIVVIDCGSKFPDESLLGIDLIIPDVTYL 59 Query: 62 TENRDKVRGIVLTHGHEDHIGGLSYVLKNLNVPVYGTRLTLGLVENKLKEANLLGETKRI 121 EN++K+RG+V+THGHEDHIGG+ Y LK LNVP+Y TRLTLGL+E KLKE NL +T+ I Sbjct: 60 QENKEKIRGLVVTHGHEDHIGGIPYFLKQLNVPIYATRLTLGLIEIKLKEHNLQNDTELI 119 Query: 122 LINEDSEIELGSSLKVTFFRTNHSIPDSVGVCIETPEGNVVHTGDFKFDHTPVNGQFANL 181 +I+ +SEI+ GS +K TFF+TNHSIPD +G+ TPEG VVHTGDFKFD TPVN Q ++ Sbjct: 120 VIHSESEIDFGS-IKTTFFKTNHSIPDCLGIAFHTPEGTVVHTGDFKFDLTPVNNQQPDI 178 Query: 182 HRMAEIGQRGVLALMSDSTNAEKPGFTPSEKNVGIVLEDIFRKAEQRVVVATFASNVHRI 241 H+MA+IG GVLAL+S+STNAE+PGFTPSE++VG +E+IF KA ++V+++TFASNV+R+ Sbjct: 179 HKMAKIGSEGVLALLSESTNAERPGFTPSERSVGERIEEIFMKANRKVIISTFASNVNRV 238 Query: 242 QQVVNAAESTGRKITVIGRSMVNVVSIASELGYLHVPDGMLIEPEEMNRMAGNRVVVLCX 301 QQ+V A T RK+ ++GRSMVNVV +A E GYL++PDGMLIE E+NR+ +V +LC Sbjct: 239 QQIVEACIKTNRKLALLGRSMVNVVEVALEKGYLNIPDGMLIEASEVNRLDPKQVAILC- 297 Query: 302 XXXGSQGEPMSALTRMARSSHRKVDILPGDTVIIAATPVPGNEKYVGRTIDELFRLGANV 361 GSQGEPM+AL R+A ++R+VD+LP DTVIIAATP+PGNE+ V R ID LF LGA V Sbjct: 298 --TGSQGEPMAALARLASGNYRQVDVLPEDTVIIAATPIPGNERNVSRIIDNLFALGAKV 355 Query: 362 IY-XXXXXXXXXXXXXXQEELKLMLNLMKPKYFLPIHGEFRMQRRHALLAESVGVEPANI 420 IY QEELKLML LMKPKYF+PIHGEFRM H+LLAES+GVE NI Sbjct: 356 IYGSGSSTGVHVSGHAYQEELKLMLALMKPKYFIPIHGEFRMLHHHSLLAESIGVEKENI 415 Query: 421 FITELGEVIEISGGSARRAGKVTAXXXXXXXXXXXXXXXXXLRDRKLLSQDGILVVVVTL 480 FI G+V++IS A ++ K+ A LRDRK LS+DG+LV+V+T Sbjct: 416 FIVRNGDVVDISNEVAIQSRKIQAGNIYVDGLGIGDVGNALLRDRKQLSEDGMLVIVITF 475 Query: 481 SKQNGAIVSGPDIISRGFVYVRESEGLLDEANRIVSGTLQRLMSEKVNEWASLKTSVKDS 540 +K +G I+SGPDIISRGFVYVR+SE L E N++ T+ L EKVN W LK V+++ Sbjct: 476 NKADGDIISGPDIISRGFVYVRDSEEFLKELNKLAVITINNLKKEKVNSWGILKREVREA 535 Query: 541 LGRFLYEQTRR 551 LG+++Y + R+ Sbjct: 536 LGKYIYIRIRK 546 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.137 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 753,536,145 Number of extensions: 30193334 Number of successful extensions: 78618 Number of sequences better than 1.0e-05: 555 Number of HSP's gapped: 77320 Number of HSP's successfully gapped: 556 Length of query: 562 Length of database: 757,237,578 Length adjustment: 131 Effective length of query: 431 Effective length of database: 401,559,609 Effective search space: 173072191479 Effective search space used: 173072191479 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)