BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0280.0021.p (287 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_832505.1| Cell wall hydrolase cwlJ [Bacillus cereus ATCC ... 254 1e-66 ref|NP_979085.1| spore cortex-lytic enzyme prepeptide [Bacillus ... 254 2e-66 ref|NP_656634.1| PG_binding_1, Putative peptidoglycan binding do... 252 5e-66 ref|NP_692727.1| sporulation specific N-acetylmuramoyl-L-alanine... 239 6e-62 ref|NP_390174.1| spore cortex-lytic enzyme [Bacillus subtilis] >... 228 8e-59 >ref|NP_832505.1| Cell wall hydrolase cwlJ [Bacillus cereus ATCC 14579] sp|P70874|SLEB_BACCR Spore cortex-lytic enzyme precursor (Germination-specific amidase) (SCLE) dbj|BAA09800.1| spore cortex-lytic enzyme prepeptide [Bacillus cereus] gb|AAP09706.1| Cell wall hydrolase cwlJ [Bacillus cereus ATCC 14579] Length = 259 Score = 254 bits (649), Expect = 1e-66, Method: Compositional matrix adjust. Identities = 133/246 (54%), Positives = 165/246 (67%), Gaps = 6/246 (2%) Query: 41 SVMTEGIVPEEEALPVFGTTPLKVGSSGQDVYELQGRLKHLGYYAGAIDSQFGAKTRNAV 100 S+M I + + + F ++ G+SG+DV ELQ RLK+ G+Y G +D FG T A+ Sbjct: 19 SLMVSSI--QLKNVEAFSNQVIQRGASGEDVIELQSRLKYNGFYTGKVDGVFGWGTYWAL 76 Query: 101 TWFQWKFGMEADGIVGAKTKLKLYNATKAWRPTEPSTSTANKTEXXXXXXXXXXXXXXXX 160 FQ KFG+ DG+ GAKTK L ATK + T +T NK Sbjct: 77 RNFQEKFGLPVDGLAGAKTKQMLVKATKYDKSTANKGTTTNKGNSGGTAQENKPPQNKGT 136 Query: 161 ELSSGNTMGLSENDLRIMANAVYGESRGEPFEGQVAVAAVILNRVKSPNFPNTPSGVIFQ 220 + +G S+ND+++MANAVYGESRGEP+ GQVAVAAVILNRV S +FPNT SGVIF+ Sbjct: 137 NVPNG----YSQNDIQLMANAVYGESRGEPYLGQVAVAAVILNRVTSASFPNTVSGVIFE 192 Query: 221 PGAFTAVADGQIYLEPNEQARKAVQQALNGWDPSGGCIYYFNPKTATSKWIWSRPQVKTI 280 P AFTAVADGQIYL PNE A+KAV A+NGWDP+G +YYFNP TATSKWIW+RPQ+K I Sbjct: 193 PRAFTAVADGQIYLTPNETAKKAVLDAINGWDPTGNALYYFNPDTATSKWIWTRPQIKKI 252 Query: 281 GQHIFC 286 G+HIFC Sbjct: 253 GKHIFC 258 >ref|NP_979085.1| spore cortex-lytic enzyme prepeptide [Bacillus cereus ATCC 10987] gb|AAS41693.1| spore cortex-lytic enzyme prepeptide [Bacillus cereus ATCC 10987] Length = 259 Score = 254 bits (648), Expect = 2e-66, Method: Compositional matrix adjust. Identities = 130/230 (56%), Positives = 158/230 (68%), Gaps = 4/230 (1%) Query: 57 FGTTPLKVGSSGQDVYELQGRLKHLGYYAGAIDSQFGAKTRNAVTWFQWKFGMEADGIVG 116 F ++ G+SG+DV ELQ RLK+ G+Y G +D FG T A+ FQ KFG+ DG+ G Sbjct: 33 FSNQVIQRGASGEDVIELQSRLKYNGFYTGKVDGVFGWGTYWALRNFQEKFGLPVDGLAG 92 Query: 117 AKTKLKLYNATKAWRPTEPSTSTANKTEXXXXXXXXXXXXXXXXELSSGNTMGLSENDLR 176 AKTK L ATK + T +T NK + +G S+ND++ Sbjct: 93 AKTKQMLVKATKYDKSTANKGTTTNKGNSGGTAQENKPPQNKGTNVPNG----YSQNDIQ 148 Query: 177 IMANAVYGESRGEPFEGQVAVAAVILNRVKSPNFPNTPSGVIFQPGAFTAVADGQIYLEP 236 +MANAVYGESRGEP+ GQVAVAAVILNRV S +FPNT SGVIF+P AFTAVADGQIYL P Sbjct: 149 LMANAVYGESRGEPYLGQVAVAAVILNRVTSASFPNTVSGVIFEPRAFTAVADGQIYLTP 208 Query: 237 NEQARKAVQQALNGWDPSGGCIYYFNPKTATSKWIWSRPQVKTIGQHIFC 286 NE A+KAV A+NGWDP+G +YYFNP TATSKWIW+RPQ+K IG+HIFC Sbjct: 209 NETAKKAVLDAINGWDPTGNALYYFNPDTATSKWIWTRPQIKKIGKHIFC 258 >ref|NP_656634.1| PG_binding_1, Putative peptidoglycan binding domain [Bacillus anthracis A2012] ref|NP_845098.1| spore cortex-lytic enzyme prepeptide [Bacillus anthracis str. Ames] gb|AAP26584.1| spore cortex-lytic enzyme prepeptide [Bacillus anthracis str. Ames] Length = 253 Score = 252 bits (644), Expect = 5e-66, Method: Compositional matrix adjust. Identities = 134/246 (54%), Positives = 164/246 (66%), Gaps = 12/246 (4%) Query: 41 SVMTEGIVPEEEALPVFGTTPLKVGSSGQDVYELQGRLKHLGYYAGAIDSQFGAKTRNAV 100 S+M I + + + F ++ G+SG+DV ELQ RLK+ G+Y G +D FG T A+ Sbjct: 19 SLMVSSI--QLKNVEAFSNQVIQRGASGEDVIELQSRLKYNGFYTGKVDGVFGWGTYWAL 76 Query: 101 TWFQWKFGMEADGIVGAKTKLKLYNATKAWRPTEPSTSTANKTEXXXXXXXXXXXXXXXX 160 FQ KFG+ DG+ GAKTK L ATK STANK Sbjct: 77 RNFQEKFGLPVDGLAGAKTKQMLVKATKY------DKSTANKGNSGGTAQENKPSQNKGT 130 Query: 161 ELSSGNTMGLSENDLRIMANAVYGESRGEPFEGQVAVAAVILNRVKSPNFPNTPSGVIFQ 220 + +G S+ND+++MANAVYGESRGEP+ GQVAVAAVILNRV S +FPNT SGVIF+ Sbjct: 131 NVPNG----YSQNDIQLMANAVYGESRGEPYLGQVAVAAVILNRVTSASFPNTVSGVIFE 186 Query: 221 PGAFTAVADGQIYLEPNEQARKAVQQALNGWDPSGGCIYYFNPKTATSKWIWSRPQVKTI 280 P AFTAVADGQIYL PNE A+KAV A+NGWDP+G +YYFNP TATSKWIW+RPQ+K I Sbjct: 187 PRAFTAVADGQIYLTPNETAKKAVLDAINGWDPTGNALYYFNPDTATSKWIWTRPQIKKI 246 Query: 281 GQHIFC 286 G+HIFC Sbjct: 247 GKHIFC 252 >ref|NP_692727.1| sporulation specific N-acetylmuramoyl-L-alanine amidase [Oceanobacillus iheyensis HTE831] sp|P59105|SLEB_OCEIH Spore cortex-lytic enzyme precursor (SCLE) dbj|BAC13762.1| sporulation specific N-acetylmuramoyl-L-alanine amidase (spore cortex-lytic enzyme) [Oceanobacillus iheyensis HTE831] Length = 276 Score = 239 bits (609), Expect = 6e-62, Method: Compositional matrix adjust. Identities = 127/245 (51%), Positives = 159/245 (64%), Gaps = 17/245 (6%) Query: 57 FGTTPLKVGSSGQDVYELQGRLKHLGYYAGAIDSQFGAKTRNAVTWFQWKFGMEADGIVG 116 F ++ G+ G+DV ELQ RL++LG+Y G ID FG T A+ FQ++FGME DG+ G Sbjct: 33 FSDQVIQQGAVGEDVIELQARLQYLGFYNGKIDGVFGWGTYWALRNFQYEFGMEIDGLAG 92 Query: 117 AKTKLKLYNATKAWRPTEPSTSTANKT---------EXXXXXXXXXXXXXXXXELSSGNT 167 TK KL A+ E T NK + +G T Sbjct: 93 QTTKDKLVQASN--YDKEYVTEQINKGNQFTHYGGGDRNTDGQSNSGGGSGNSGEDTGGT 150 Query: 168 ------MGLSENDLRIMANAVYGESRGEPFEGQVAVAAVILNRVKSPNFPNTPSGVIFQP 221 G S+ND+++MANAVYGE+RGEP+EGQVAVAAVILNR+ S FP+T SGVIF+P Sbjct: 151 PSVNVPQGYSQNDIQLMANAVYGEARGEPYEGQVAVAAVILNRLNSALFPDTVSGVIFEP 210 Query: 222 GAFTAVADGQIYLEPNEQARKAVQQALNGWDPSGGCIYYFNPKTATSKWIWSRPQVKTIG 281 AFTAVADGQI+LEPN++AR+AV A+NGWDPSG +YYFNP TATS WIW+RPQ+K IG Sbjct: 211 RAFTAVADGQIWLEPNDKAREAVLDAINGWDPSGNALYYFNPDTATSGWIWTRPQIKQIG 270 Query: 282 QHIFC 286 +HIFC Sbjct: 271 KHIFC 275 >ref|NP_390174.1| spore cortex-lytic enzyme [Bacillus subtilis] sp|P50739|SLEB_BACSU Spore cortex-lytic enzyme precursor (SCLE) pir||C69708 spore cortex-lytic enzyme prepeptide [imported] - Bacillus subtilis gb|AAC83957.1| spore cortex lytic enzyme [Bacillus subtilis] dbj|BAA11473.1| spore-cortex-lytic enzyme prepeptide [Bacillus subtilis] emb|CAB14209.1| spore cortex-lytic enzyme [Bacillus subtilis subsp. subtilis str. 168] Length = 305 Score = 228 bits (582), Expect = 8e-59, Method: Compositional matrix adjust. Identities = 125/280 (44%), Positives = 160/280 (57%), Gaps = 45/280 (16%) Query: 52 EALPVFGTTPLKVGSSGQDVYELQGRLKHLGYYAGAIDSQFGAKTRNAVTWFQWKFGM-E 110 E + F ++ G++G DV ELQ RL++ GYY G ID +G T AV FQ +FG+ E Sbjct: 25 ETISAFSNQVIQRGATGDDVVELQARLQYNGYYNGKIDGVYGWGTYWAVRNFQDQFGLKE 84 Query: 111 ADGIVGAKTKLKLYNATKAWRP-------------------------------------- 132 DG+VGAKTK L +K +R Sbjct: 85 VDGLVGAKTKQTLICKSKYYREYVMEQLNKGNTFTHYGKIPLKYQTKPSKAATQKARQQA 144 Query: 133 ----TEPSTSTANKTEXXXXXXXXXXXXXXXXELSSGNTM--GLSENDLRIMANAVYGES 186 +P+ T K + + + M G S ND+R++A AVYGE+ Sbjct: 145 EARQKQPAEKTTQKPKANANKQQNNTPAKARKQDAVAANMPGGFSNNDIRLLAQAVYGEA 204 Query: 187 RGEPFEGQVAVAAVILNRVKSPNFPNTPSGVIFQPGAFTAVADGQIYLEPNEQARKAVQQ 246 RGEP+EGQVA+AAVILNR+ SP FPN+ +GVIF+P AFTAVADGQIY++PNE AR+AV Sbjct: 205 RGEPYEGQVAIAAVILNRLNSPLFPNSVAGVIFEPLAFTAVADGQIYMQPNETAREAVLD 264 Query: 247 ALNGWDPSGGCIYYFNPKTATSKWIWSRPQVKTIGQHIFC 286 A+NGWDPS +YYFNP TATS WIW RPQ+K IG+HIFC Sbjct: 265 AINGWDPSEEALYYFNPDTATSPWIWGRPQIKRIGKHIFC 304 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.315 0.131 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 404,387,583 Number of extensions: 15198675 Number of successful extensions: 23271 Number of sequences better than 1.0e-05: 78 Number of HSP's gapped: 23277 Number of HSP's successfully gapped: 88 Length of query: 287 Length of database: 757,237,578 Length adjustment: 124 Effective length of query: 163 Effective length of database: 420,565,302 Effective search space: 68552144226 Effective search space used: 68552144226 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 125 (52.8 bits)