BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0280.0020.p (412 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_980119.1| conserved hypothetical protein [Bacillus cereus... 306 5e-82 ref|NP_657754.1| hypothetical protein predicted by GeneMark [Bac... 304 3e-81 ref|NP_243259.1| BH2393~unknown conserved protein [Bacillus halo... 304 3e-81 ref|NP_833510.1| Zinc protease [Bacillus cereus ATCC 14579] >gi|... 302 1e-80 ref|NP_623254.1| predicted Zn-dependent peptidase [Thermoanaerob... 285 1e-75 >ref|NP_980119.1| conserved hypothetical protein [Bacillus cereus ATCC 10987] gb|AAS42727.1| conserved hypothetical protein [Bacillus cereus ATCC 10987] Length = 424 Score = 306 bits (785), Expect = 5e-82, Method: Compositional matrix adjust. Identities = 162/414 (39%), Positives = 249/414 (60%), Gaps = 12/414 (2%) Query: 1 MRIHVLPTKAFKTFAISLYAGVPLDEHTVTPTALIPFVLRRGTANYPETTQFRERLEELY 60 +R+H++PT +KT PL+E TVT AL+P+VL+ T P + R+ LEELY Sbjct: 14 LRVHIIPTDKYKTNTFVFRLKAPLNEETVTERALLPYVLQSATEKLPSVIRLRQYLEELY 73 Query: 61 GAGFGFDIYKRGDYQIVQFRMDTINDSFVQSKESLLGESFAFLGEVLTKPLLEDGSFRAS 120 G+ D+ K+G+ I+ +D N+ ++ L ++ + L +++ P E F S Sbjct: 74 GSSLAVDVSKKGEDHIISIYVDIANEVYLHDAPPLFEKALSMLSDIVLHPATEGNGFLPS 133 Query: 121 YVATERETVRKKLEAIVNDKIRYAAERCIEEMCRQEPYRLHPLGQRADLDGITPESLYQS 180 V +E+ + +++EA +DK+RYA ER IEEMC+ EPYRL G++ + IT ESLYQ Sbjct: 134 IVESEKRALLQRIEATYDDKMRYANERLIEEMCKVEPYRLSANGKKESVSSITNESLYQY 193 Query: 181 YHSWLGGAILDLYVVGDTTPEEVEKLVTRHFGRT-----HGNAAGYSSNFSPVAVTEVRT 235 Y L +DLY++GD + V+ LV+++F + N + N E + Sbjct: 194 YQKVLAEDEMDLYIIGDISENAVD-LVSKYFSISARPARERNVLLHKRN------NEEKE 246 Query: 236 VEEKLDVNQGKLNMGLRTPITYKDDRYASALMYNGILGGYPHSKLFVNVREKESLAYYAS 295 V EK ++ Q KL++G RT ITYKD+ Y + ++NG+ GG+ HSKLFVNVREK SLAYYA+ Sbjct: 247 VVEKQELKQSKLHIGYRTFITYKDEDYFALQLFNGLFGGFSHSKLFVNVREKNSLAYYAA 306 Query: 296 SRYDGHKGIGTIQSGIETQNYSKAVDIIRKQLDELQAGNISDLELSQTKAMIRNLLSEIQ 355 SR++ HKG+ + SGIE +NY KAV+II++Q+ +Q G+ S+ E+ QTK++IRN + E Sbjct: 307 SRFESHKGLLFVMSGIEAKNYEKAVEIIKEQMLAMQNGDFSEEEMHQTKSVIRNQILEAI 366 Query: 356 DSAFEMISFDFNRQLSGKDRSAQELLAQVDSTGAEDVKAAAGTFELDTIYFLTG 409 D+ + ++ +S + R +E L ++S E++ A ELDTIYFL G Sbjct: 367 DTPRGFVEMLYHGIISDRTRPVEEWLTGIESVTKEEIVKVAKNIELDTIYFLQG 420 >ref|NP_657754.1| hypothetical protein predicted by GeneMark [Bacillus anthracis A2012] ref|NP_846170.1| conserved hypothetical protein [Bacillus anthracis str. Ames] gb|AAP27656.1| conserved hypothetical protein [Bacillus anthracis str. Ames] Length = 424 Score = 304 bits (779), Expect = 3e-81, Method: Compositional matrix adjust. Identities = 161/414 (38%), Positives = 249/414 (60%), Gaps = 12/414 (2%) Query: 1 MRIHVLPTKAFKTFAISLYAGVPLDEHTVTPTALIPFVLRRGTANYPETTQFRERLEELY 60 +R+H++PT +KT PL+E TVT AL+P+VL+ T P + R+ LEELY Sbjct: 14 LRVHIIPTDKYKTNTFVFRLKAPLNEETVTERALLPYVLQSATEKLPSVIRLRQYLEELY 73 Query: 61 GAGFGFDIYKRGDYQIVQFRMDTINDSFVQSKESLLGESFAFLGEVLTKPLLEDGSFRAS 120 G+ D+ K+G+ I+ +D N+ ++ L ++ + L +++ P E F S Sbjct: 74 GSSLAVDVSKKGEDHIISIYVDIANEVYLHDAPPLFEKALSMLSDIVLHPATEGNGFLPS 133 Query: 121 YVATERETVRKKLEAIVNDKIRYAAERCIEEMCRQEPYRLHPLGQRADLDGITPESLYQS 180 V +E+ + +++EA +DK+RYA ER IEEMC+ EPYRL G++ + IT ESLYQ Sbjct: 134 IVESEKRALLQRIEATYDDKMRYANERLIEEMCKVEPYRLSANGKKESVASITNESLYQY 193 Query: 181 YHSWLGGAILDLYVVGDTTPEEVEKLVTRHFGRT-----HGNAAGYSSNFSPVAVTEVRT 235 Y L +DLY++GD + V+ LV+++F + N + N E + Sbjct: 194 YQKVLAEDEMDLYIIGDISENAVD-LVSKYFSISARPVRERNVLLHKRN------NEEKE 246 Query: 236 VEEKLDVNQGKLNMGLRTPITYKDDRYASALMYNGILGGYPHSKLFVNVREKESLAYYAS 295 V EK ++ Q KL++G RT ITYKD+ Y + ++NG+ GG+ HSKLFVNVREK SLAYYA+ Sbjct: 247 VVEKQELKQSKLHIGYRTFITYKDEDYFALQLFNGLFGGFSHSKLFVNVREKNSLAYYAA 306 Query: 296 SRYDGHKGIGTIQSGIETQNYSKAVDIIRKQLDELQAGNISDLELSQTKAMIRNLLSEIQ 355 SR++ HKG+ + SGIE +NY KAV+II++Q+ +Q G+ S+ E+ QTK++I+N + E Sbjct: 307 SRFESHKGLLFVMSGIEAKNYEKAVEIIKEQMLAMQNGDFSEEEIHQTKSVIQNQILEAI 366 Query: 356 DSAFEMISFDFNRQLSGKDRSAQELLAQVDSTGAEDVKAAAGTFELDTIYFLTG 409 D+ + ++ +S + R +E L ++S E++ A ELDTIYFL G Sbjct: 367 DTPRGFVEMLYHGIISDRTRPVEEWLTGIESVTKEEIVKVAKNIELDTIYFLQG 420 >ref|NP_243259.1| BH2393~unknown conserved protein [Bacillus halodurans] pir||A83949 hypothetical protein BH2393 [imported] - Bacillus halodurans (strain C-125) dbj|BAB06112.1| BH2393~unknown conserved protein [Bacillus halodurans] Length = 431 Score = 304 bits (778), Expect = 3e-81, Method: Compositional matrix adjust. Identities = 158/415 (38%), Positives = 242/415 (58%), Gaps = 5/415 (1%) Query: 1 MRIHVLPTKAFKTFAISLYAGVPLDEHTVTPTALIPFVLRRGTANYPETTQFRERLEELY 60 + +H+L T +KT + L PL++ TV AL+ VL+ TA P + R RL++LY Sbjct: 14 LHVHLLKTDKYKTNTLMLMVKAPLEKETVAKRALLANVLQNSTAKSPTRSDLRNRLDDLY 73 Query: 61 GAGFGFDIYKRGDYQIVQFRMDTINDSFVQSKESLLGESFAFLGEVLTKPLLEDGSFRAS 120 GA D+ K+G+ I+ R+D N+ F+ L + + L EVL +P + F + Sbjct: 74 GATLATDVQKKGEQHIITIRLDVANEEFLSDSTPLFEKGLSLLAEVLLEPKVSGNKFDEA 133 Query: 121 YVATERETVRKKLEAIVNDKIRYAAERCIEEMCRQEPYRLHPLGQRADLDGITPESLYQS 180 V +E+ ++ +++E++ +DK+RY++ R EEMC+ EP+ L G R ++ ++ E LY+ Sbjct: 134 VVKSEKRSLLERIESLYDDKMRYSSLRVTEEMCKDEPFGLSVYGTREQVEQLSAEELYRY 193 Query: 181 YHSWLGGAILDLYVVGDTTPEEVEKLVTRHFGRTHGNAAGYSSNFSPVA-----VTEVRT 235 Y L +DL+V+G+ + V F + ++ P + V E R Sbjct: 194 YEQMLHSDRIDLFVLGEKGDPSLMSTVEDTFSKLATYQKEAKTDSVPASIPSPEVKEARE 253 Query: 236 VEEKLDVNQGKLNMGLRTPITYKDDRYASALMYNGILGGYPHSKLFVNVREKESLAYYAS 295 V EK DV QGKLN+G R TY D Y + + NG+ GG+ HSKLF+NVREKESLAYYA+ Sbjct: 254 VIEKQDVKQGKLNIGFRAYTTYGDPDYVAMQVMNGLFGGFSHSKLFINVREKESLAYYAA 313 Query: 296 SRYDGHKGIGTIQSGIETQNYSKAVDIIRKQLDELQAGNISDLELSQTKAMIRNLLSEIQ 355 SR++ HKG+ + SGIE NY KAV II++QL+E++ G +D EL QT+AM++N L E Sbjct: 314 SRFESHKGLLIVMSGIEFTNYEKAVTIIKEQLEEMKNGRFTDKELEQTRAMLKNQLLETA 373 Query: 356 DSAFEMISFDFNRQLSGKDRSAQELLAQVDSTGAEDVKAAAGTFELDTIYFLTGK 410 D I ++ +S +R+ ++ LA++D+ E V AAA LDTIYFL G+ Sbjct: 374 DVPRGQIEVMYHNIVSNHERTLEKWLAEIDAITKEQVVAAANKVVLDTIYFLKGE 428 >ref|NP_833510.1| Zinc protease [Bacillus cereus ATCC 14579] gb|AAP10711.1| Zinc protease [Bacillus cereus ATCC 14579] Length = 424 Score = 302 bits (774), Expect = 1e-80, Method: Compositional matrix adjust. Identities = 160/414 (38%), Positives = 249/414 (60%), Gaps = 12/414 (2%) Query: 1 MRIHVLPTKAFKTFAISLYAGVPLDEHTVTPTALIPFVLRRGTANYPETTQFRERLEELY 60 +R+H++PT +KT PL+E TVT AL+P+VL+ T P + R+ LEELY Sbjct: 14 LRVHIIPTDKYKTNTFVFRFKAPLNEETVTERALLPYVLQSATEKLPSVIRLRQYLEELY 73 Query: 61 GAGFGFDIYKRGDYQIVQFRMDTINDSFVQSKESLLGESFAFLGEVLTKPLLEDGSFRAS 120 G+ D+ K+G+ I+ +D N+ ++ L ++ + L +++ P E F +S Sbjct: 74 GSSLAVDVSKKGEDHIISIYVDIANEVYLHDAPPLFEKALSMLSDIVLHPATEGNGFLSS 133 Query: 121 YVATERETVRKKLEAIVNDKIRYAAERCIEEMCRQEPYRLHPLGQRADLDGITPESLYQS 180 V +E+ + +++EA +DK+RYA ER IEEMC+ EPYRL G++ + IT ESLYQ Sbjct: 134 IVESEKRALLQRIEATYDDKMRYANERLIEEMCKVEPYRLSANGKKESVMSITNESLYQY 193 Query: 181 YHSWLGGAILDLYVVGDTTPEEVEKLVTRHFGRT-----HGNAAGYSSNFSPVAVTEVRT 235 Y L +DLY++GD + V+ LV+++F + N + N E + Sbjct: 194 YQKVLAEDEMDLYIIGDISENAVD-LVSKYFSISTRPVRERNVLLHRRN------NEEKE 246 Query: 236 VEEKLDVNQGKLNMGLRTPITYKDDRYASALMYNGILGGYPHSKLFVNVREKESLAYYAS 295 V EK ++ Q KL++G RT +TYKD+ Y + ++NG+ GG+ HSKLFVNVREK SLAYYA+ Sbjct: 247 VVEKQELKQSKLHIGYRTFVTYKDEDYFALQLFNGLFGGFSHSKLFVNVREKNSLAYYAA 306 Query: 296 SRYDGHKGIGTIQSGIETQNYSKAVDIIRKQLDELQAGNISDLELSQTKAMIRNLLSEIQ 355 SR++ HKG+ + SGIE +NY KAV+II++Q+ +Q G S+ E+ QTK++I+N + E Sbjct: 307 SRFESHKGLLFVMSGIEAKNYEKAVEIIKEQMLAMQNGEFSEEEIHQTKSVIQNQILEAI 366 Query: 356 DSAFEMISFDFNRQLSGKDRSAQELLAQVDSTGAEDVKAAAGTFELDTIYFLTG 409 D+ + ++ +S + R +E L ++S E++ A ELDTIYFL G Sbjct: 367 DTPRGFVEMLYHGIISDRTRPVEEWLTGIESVTKEEIVKVAKNIELDTIYFLQG 420 >ref|NP_623254.1| predicted Zn-dependent peptidase [Thermoanaerobacter tengcongensis] gb|AAM24858.1| predicted Zn-dependent peptidase [Thermoanaerobacter tengcongensis] Length = 421 Score = 285 bits (730), Expect = 1e-75, Method: Compositional matrix adjust. Identities = 151/412 (36%), Positives = 238/412 (57%), Gaps = 3/412 (0%) Query: 1 MRIHVLPTKAFKTFAISLYAGVPLDEHTVTPTALIPFVLRRGTANYPETTQFRERLEELY 60 + +++ T FKT ++LY L + T ALIP VL+RGT+ + LE LY Sbjct: 12 INLYLDVTDKFKTVTVNLYVQNKLGDEA-TWYALIPSVLKRGTSALKTYKDMVKYLEMLY 70 Query: 61 GAGFGFDIYKRGDYQIVQFRMDTINDSFVQSKESLLGESFAFLGEVLTKPLLEDGSFRAS 120 G +YK+G+ Q+R++ + +V E++ + FL E++ +P +DG+F Sbjct: 71 GTTMAVSVYKKGERHFQQYRLELPQEEYVS--ENVFEKGIKFLYELVFEPFTQDGAFLKE 128 Query: 121 YVATERETVRKKLEAIVNDKIRYAAERCIEEMCRQEPYRLHPLGQRADLDGITPESLYQS 180 YV E+E + +E+ +NDK +YA +RC EEMC+ EP+ + LG++ DLD I ++L++ Sbjct: 129 YVLQEKEVQKNLIESRINDKTKYAVDRCYEEMCKGEPFAIFELGKKEDLDLIDEKNLFEY 188 Query: 181 YHSWLGGAILDLYVVGDTTPEEVEKLVTRHFGRTHGNAAGYSSNFSPVAVTEVRTVEEKL 240 Y + +D+YVVG+ PE E++ ++F V EV+ V E+L Sbjct: 189 YKKCIDTLPVDIYVVGNVNPEYAEEVFRKYFNLRRKEVLEIPFTDVRKEVKEVKYVTEEL 248 Query: 241 DVNQGKLNMGLRTPITYKDDRYASALMYNGILGGYPHSKLFVNVREKESLAYYASSRYDG 300 DVNQGKL +G RT + + Y L+Y+ +LGG P SKLF+NVREK SLAYYA SR + Sbjct: 249 DVNQGKLTLGFRTNVPPDSEEYYPLLVYSTVLGGGPFSKLFINVREKASLAYYAYSRLER 308 Query: 301 HKGIGTIQSGIETQNYSKAVDIIRKQLDELQAGNISDLELSQTKAMIRNLLSEIQDSAFE 360 KG+ I SGIE +NYSKA+DII K++ E++ GNISD E K + L+ I+D+A Sbjct: 309 FKGLMVISSGIEVENYSKALDIILKEVGEMEKGNISDYEFDSAKKSLYTSLNAIKDNATS 368 Query: 361 MISFDFNRQLSGKDRSAQELLAQVDSTGAEDVKAAAGTFELDTIYFLTGKGE 412 + +++++G + +E + +++ EDV + +LDT+YF+ K E Sbjct: 369 KADYYLSQKIAGTNLGIEEFIKKIEKVSKEDVVEVSKKVKLDTVYFMRNKKE 420 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.317 0.135 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 597,317,591 Number of extensions: 24997578 Number of successful extensions: 63206 Number of sequences better than 1.0e-05: 106 Number of HSP's gapped: 63259 Number of HSP's successfully gapped: 106 Length of query: 412 Length of database: 757,237,578 Length adjustment: 128 Effective length of query: 284 Effective length of database: 409,704,906 Effective search space: 116356193304 Effective search space used: 116356193304 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 127 (53.5 bits)