BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0274.0007.p (577 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00123196.1| COG1472: Beta-glucosidase-related glycosidase... 243 7e-63 ref|NP_561070.1| probable beta-hexosamidase A [Clostridium perfr... 235 2e-60 ref|NP_735128.1| Unknown [Streptococcus agalactiae NEM316] >gi|2... 206 2e-51 gb|AAQ05801.1| N-acetyl-beta-glucosaminidase [Cellulomonas fimi] 205 2e-51 ref|NP_687720.1| glycosyl hydrolase, family 3 [Streptococcus aga... 205 3e-51 >ref|ZP_00123196.1| COG1472: Beta-glucosidase-related glycosidases [Haemophilus somnus 129PT] Length = 586 Score = 243 bits (621), Expect = 7e-63, Method: Compositional matrix adjust. Identities = 180/584 (30%), Positives = 268/584 (45%), Gaps = 48/584 (8%) Query: 7 LTREEQDWVERTLTSMSLREKIGQTMQDHAGRLPFKGNDEEALAAYLKTYPVGSFFIGGE 66 L E+ WV+ T+ +M+L EKIGQ + EE L L Y +G+ Sbjct: 12 LKDEDIQWVKNTIANMTLEEKIGQLFVNMGS-----SRSEEYLTDVLNRYHIGA------ 60 Query: 67 VIQKAAGKAEEYRDWAEQLQRISKYPLLFSGDLEFGAGSAVKSLTAFPPXXXXXXXXXXX 126 ++ G AEE D LQ SK PLL + + E G A T Sbjct: 61 -VRYNPGPAEEVYDQNYILQTKSKIPLLIAANTEAGGNGACSDGTEIGLQVKIGATDDAK 119 Query: 127 XXXXXXKFTALEGRAAGFTWALAPGTDLLLNWMNPVITTRCXXXXXXXXXXXXXXVMRGM 186 + +E A G W+ AP D+ NW NP+I+ R M+G+ Sbjct: 120 YAYEMGRVAGIEAAAIGCNWSFAPIVDISYNWRNPIISNRVFGSNPEKVLEMSLAYMKGI 179 Query: 187 QDHGLAACAKHFPGDGVDYRDQHIVTTINGLSEEEWFAAYGQVSQHMIDQGVLSYMTGHI 246 Q+ G+A AKHFPGDGVD RDQH+ ++N S EEW +G+V Q +ID G+ S M GHI Sbjct: 180 QESGIAPAAKHFPGDGVDERDQHLSFSVNSFSCEEWDNTFGKVYQGLIDAGLPSLMAGHI 239 Query: 247 ALPWLEG----GTQEEKPVPATVSERITTGLLRERMGFAGVVLSDALDMGGFLAWGSYEK 302 LP E E +PAT+S+ I T LLR ++GF GVV++DA M + + Sbjct: 240 HLPAYEQHFNPNLAYEDCLPATLSKPILTDLLRGKLGFNGVVVTDASHMVAMTSAMKRSE 299 Query: 303 RMVDCFNSGTDVLLWPG--VEYFGVMEQAVSEGTVLMERLDASVRRVLELKALLGVH-RV 359 + +G D+ L+ E FG M + G + ERL ++ R+L LKA LG+H R Sbjct: 300 MLPTAIAAGCDLFLFFNDPDEDFGYMMEGYKNGIITEERLHDALTRILGLKAKLGLHNRP 359 Query: 360 DQKGRDPQAVPLLNDRLPPKLDLEARQLSRTIAERCITLVRNRGGILPLDPQTTRRVLVV 419 + +P+ L LP E + + R +A++ ITLV+++ I P++ + R+L+V Sbjct: 360 RETLLEPKEQALAKIGLP-----EHKAIFRDVADKAITLVKHKQDIFPINVERFPRILLV 414 Query: 420 LLN-------KVTEGRKYERMNVFVELLRARGLQVDI----------LDEFEPLGTLRKW 462 + K+ G + + E L +G V I + + E + T+R Sbjct: 415 NVKGTDGGFGKMVAGSQRSATEILKEKLEEKGFTVSIYTSPMDNILKMSDEEQVKTIRN- 473 Query: 463 ELSGIRWDAAFAPYFLPLHGMMN-----TARPVGEAAKAIWAMQ-HAETIRPIGISFATP 516 E S R ++ + + N R V +A K + + + I +S P Sbjct: 474 EYSQKRPITTLTDHYDLIINVANVQMSTVQRIVWQATKGTPDIPFYVHEVPTIFVSVQCP 533 Query: 517 YLLQDMPFLDALINAYSLHEETVELTVKALFGEVPFQGQSPVKA 560 + L D+P + INAY E T+EL V+ L G F+G SPV A Sbjct: 534 FHLVDVPQVKTYINAYDGKEPTIELLVEKLMGNSEFKGISPVDA 577 >ref|NP_561070.1| probable beta-hexosamidase A [Clostridium perfringens] dbj|BAB79860.1| probable beta-hexosamidase A [Clostridium perfringens str. 13] Length = 589 Score = 235 bits (600), Expect = 2e-60, Method: Compositional matrix adjust. Identities = 178/589 (30%), Positives = 270/589 (45%), Gaps = 54/589 (9%) Query: 7 LTREEQDWVERTLTSMSLREKIGQTMQDHAGRLPFKGNDEEALAAYLKTYPVGSFFIGGE 66 L E WVE T+ +MS+ EKIGQ + DEE L + L Y +G+ Sbjct: 11 LDEESIKWVEDTIKNMSIEEKIGQLFVNMGA-----SRDEEYLKSVLNNYHIGA------ 59 Query: 67 VIQKAAGKAEEYRDWAEQLQRISKYPLLFSGDLEFGAGSAVKSLTAFPPXXXXXXXXXXX 126 ++ GKAEE + LQ SK PLL + + E G A T Sbjct: 60 -VRYNPGKAEEVYEQNRILQENSKIPLLIAANTEAGGNGACTDGTYVGNEVKIAATQDPH 118 Query: 127 XXXXXXKFTALEGRAAGFTWALAPGTDLLLNWMNPVITTRCXXXXXXXXXXXXXXVMRGM 186 + + +E A G W+ AP D+ NW NP+I+TR MRG+ Sbjct: 119 YAYELGRISGIEASAIGCNWSFAPIVDINRNWRNPIISTRTWSADADQTLELSLEYMRGI 178 Query: 187 QDHGLAACAKHFPGDGVDYRDQHIVTTINGLSEEEWFAAYGQVSQHMIDQGVLSYMTGHI 246 + G+ AKHFPGDG+D RDQH+ N LS EEW A +G+V +I+ G+ S M GHI Sbjct: 179 MESGILPAAKHFPGDGIDERDQHLSFAPNTLSVEEWNATFGKVYSGLIEAGLPSIMAGHI 238 Query: 247 ALP-----WLEGGTQEEKPVPATVSERITTGLLRERMGFAGVVLSDALDMGGFLAWGSYE 301 ALP + T +E +PAT+S+ I T LL+ +MGF G+V++DA M + + Sbjct: 239 ALPEYVKYFNPDATIKEMNMPATLSKYILTDLLKGQMGFNGLVVTDASHMVAMTSAMKRK 298 Query: 302 KRMVDCFNSGTDVLLWPG--VEYFGVMEQAVSEGTVLMERLDASVRRVLELKALLGVHRV 359 + +G+D+ L+ E F M + G + ERL ++ R+L LKA LG+H+ Sbjct: 299 DMLPTAIAAGSDLFLFFNDPDEDFQWMMEGYKNGVITEERLHDALTRILGLKASLGLHK- 357 Query: 360 DQKGRDPQAVPLLNDRLPPKLDLEA-RQLSRTIAERCITLVRNRGGILPLDPQTTRRVLV 418 K + P + K+ LE ++ + IA++ ITLV+N I P+ P T+RVL+ Sbjct: 358 --KAKTEILQP--KEEAMAKIGLEENKKAALEIADKAITLVKNNEEIFPISPDKTKRVLL 413 Query: 419 VLLN----------KVTEGRKYERMNVFVELLRARGLQVDILDE-FEPLGTLRKWEL--- 464 V + + +G + +++ ELL G QV+I + + + L K E+ Sbjct: 414 VKVKGFDGGFGKFLALMQGAQKGPVDIMKELLEQEGFQVEIYESPVDKIMKLPKEEMGAA 473 Query: 465 -----SGIRWDAAFAPYFLPLHGMMNTARPVGEAAKAIWAMQ--------HAETIRPIGI 511 +G R A + + + + V + + W + I I I Sbjct: 474 IGNIYAGKRPIAELTSKYDLIINLADVGASVDQRLQ--WPPSKGTPDIPFYVNEIPTIFI 531 Query: 512 SFATPYLLQDMPFLDALINAYSLHEETVELTVKALFGEVPFQGQSPVKA 560 S P+ L D+P + INAY E ++ V + G F+G S V A Sbjct: 532 SLQCPFHLADVPQVKTYINAYDSKEFNIKALVDKMMGRSEFKGVSSVDA 580 >ref|NP_735128.1| Unknown [Streptococcus agalactiae NEM316] emb|CAD46322.1| Unknown [Streptococcus agalactiae NEM316] Length = 596 Score = 206 bits (523), Expect = 2e-51, Method: Compositional matrix adjust. Identities = 161/593 (27%), Positives = 257/593 (43%), Gaps = 62/593 (10%) Query: 7 LTREEQDWVERTLTSMSLREKIGQTMQDHAGRLPFKGNDEEALAAYLKTYPVGSFFIGGE 66 L +E +W+E+T+ M+L EKIGQ + EE L L Y + + Sbjct: 14 LNQEAIEWIEKTINEMTLDEKIGQLFFNMGA-----SRSEEYLTDVLDRYHIAA------ 62 Query: 67 VIQKAAGKAEEYRDWAEQLQRISKYPLLFSGDLEFGAGSAVKSLTAFPPXXXXXXXXXXX 126 ++ G + E D LQ SK P+L + + E G AV T Sbjct: 63 -VRYNRGSSSEIYDQNLILQTKSKLPMLIAANTEAGGDGAVTDGTKVGDEIKVAATNDPK 121 Query: 127 XXXXXXKFTALEGRAAGFTWALAPGTDLLLNWMNPVITTRCXXXXXXXXXXXXXXVMRGM 186 + +E A G + AP DL NW NP+I +R M+G+ Sbjct: 122 YAYEMGRIAGMEASAVGCNASFAPIVDLTRNWRNPIIASRNWGANVDQIISLSKEYMKGI 181 Query: 187 QDHGLAACAKHFPGDGVDYRDQHIVTTINGLSEEEWFAAYGQVSQHMIDQGVLSYMTGHI 246 + + AKHFPGDG+D RD H+ N +S+EEW + +G++ + D G+ M GHI Sbjct: 182 MQYNIVPFAKHFPGDGIDERDHHLSFASNPMSKEEWMSTFGRIYGELADAGLPGVMAGHI 241 Query: 247 ALPWLEGGTQEEKP----VPATVSERITTGLLRERMGFAGVVLSDALDMGGFLAWGSYEK 302 LP +E E+ +PA++++ + LLR +G+ G +++DA M G A S Sbjct: 242 HLPNVEKEMHPERDLDDMLPASLNKTLLDELLRGELGYNGAIVTDASHMVGMTASMSRRD 301 Query: 303 RMVDCFNSGTDVLLWPG--VEYFGVMEQAVSEGTVLMERLDASVRRVLELKALLGVHRVD 360 + +G D+ L+ E M++ +G + ERL ++RR L LKA LG+H + Sbjct: 302 LLPTAIEAGCDLFLFFNDPDEDIQWMKEGYEKGILTEERLHDALRRTLGLKAKLGLHNYE 361 Query: 361 QKGRD-----PQAVPLLNDRLPPKLDLEARQLSRTIAERCITLVRNR-GGILPLDPQTTR 414 + ++ +A+ L+N E+++++ +A++ +TLV+++ I P++P+ R Sbjct: 362 GRRQELFMPKDKAMALINTS-------ESQKIADEVADKAVTLVKDKQKDIFPVNPERYR 414 Query: 415 RVLVVLLN-------KVTEGRKYERMNVFVELLRARGLQVDILDEFEPLGTLRKWELSGI 467 +L+V + + G K + ELL ARG +V + + E R +L Sbjct: 415 HILLVNVEGYKGGFGAMIAGNKQRASDYMKELLEARGHEVTVWESTEE----RIMKLPQE 470 Query: 468 RWDAAFAPYFLPLHGMMN------------TARPVGEAAKAIWAMQHAETIRP------- 508 AA A + + N G + IW +P Sbjct: 471 ERAAAIANVYAQKQPIANLTDRYDLIINLVDVNAGGTTQRIIWPAAKGTPDQPFYVHEIP 530 Query: 509 -IGISFATPYLLQDMPFLDALINAYSLHEETVELTVKALFGEVPFQGQSPVKA 560 I IS + L DMP + INAY T+ V L GE F G SPV A Sbjct: 531 SIVISVQHAFALADMPQVGTYINAYDGLPSTISAVVAKLAGESEFTGVSPVDA 583 >gb|AAQ05801.1| N-acetyl-beta-glucosaminidase [Cellulomonas fimi] Length = 564 Score = 205 bits (522), Expect = 2e-51, Method: Compositional matrix adjust. Identities = 172/564 (30%), Positives = 242/564 (42%), Gaps = 43/564 (7%) Query: 14 WVERTLTSMSLREKIGQTMQDHAGRLPFKGNDEEALAAYLKTYPVGSFFIGGEVIQKAAG 73 WV TL M EK+GQ L +D E L Y +GG +++ Sbjct: 18 WVRTTLAEMGEDEKLGQLFC-----LITYTSDPEYLG-----YLTRGLHVGGVMLRTMTA 67 Query: 74 KAEEYRDWAEQLQRISKYPLLFSGDLEFGAGSAVKSLTAFPPXXXXXXXXXXXXXXXXXK 133 LQ + PLL S +LE GA V+ T Sbjct: 68 ADAAAT--VTTLQSTATVPLLISANLEGGASQTVQEATHVGSNMALAATGSTDHVRRAAT 125 Query: 134 FTALEGRAAGFTWALAPGTDLLLNWMNPVITTRCXXXXXXXXXXXXXXVMRGMQDHGLAA 193 E RA G WA P D+ LN+ NP+ TR + +Q GLAA Sbjct: 126 VIGREARALGINWAFTPVVDIDLNFRNPITNTRTFGADAATVAAMGAEYVEAIQAQGLAA 185 Query: 194 CAKHFPGDGVDYRDQHIVTTINGLSEEEWFAAYGQVSQHMIDQGVLSYMTGHIALP---- 249 AKHFPGDGVD RDQH++ ++N +S EEW ++G V + I GV + M GHI LP Sbjct: 186 SAKHFPGDGVDERDQHLLASVNTMSVEEWDDSFGVVYRAAIAAGVKTVMVGHIMLPAYSR 245 Query: 250 WLEGGTQEEKPVPATVSERITTGLLRERMGFAGVVLSDALDMGGFLAWGSYEKRMVDCFN 309 L G + +P V+E + LLR+R+GF G+V+SD+ M G + + + Sbjct: 246 ALRPGVADRDILPGVVAEELLNDLLRDRLGFNGLVVSDSTTMAGLASVLPRSQAVPRVIA 305 Query: 310 SGTDVLLWPGV--EYFGVMEQAVSEGTVLMERLDASVRRVLELKALLGVHRVDQKGRDPQ 367 +G D+ L+ E FG M + +G + ERLD +V R+L LKA LG+HR Sbjct: 306 AGCDMFLFTKNLDEDFGYMRAGIRDGVITPERLDEAVTRILALKASLGLHRGTNLPAQGA 365 Query: 368 AVPLLNDRLPPKLDLEARQLSRTIAERCITLVRNRGGILPLDPQTTRRVLVVLLNK---- 423 A L D + +R +A ITLV+ G+LP+ + RVLV L Sbjct: 366 AGVL--------ADPDHSATAREVAASSITLVKEEPGVLPITRERYPRVLVYDLQNGGSP 417 Query: 424 VTEGRKYERMNVFVELLRARGLQVDILDEFEPLGTLRKWELSGIRWDAAFAPYFLPLHGM 483 + +G + + FV+ L G V FEP G WE + L L+ Sbjct: 418 IGQGARAGAVEQFVDALVEAGHDV---TRFEPGG---GWEGMAAPTTDVTERHDLVLYLA 471 Query: 484 MNTARPVGEAAKAIWAM-------QHAETIRPIGISFATPYLLQDMPFLDALINAYSLHE 536 + R + WA + ++ + +SF PY L D+P + LIN Y Sbjct: 472 NLSTRSNQTVVRIEWAEPMGANVPAYVHSVPTVFVSFENPYHLFDVPRVRTLINTYGSSP 531 Query: 537 ETVELTVKALFGEVPFQGQSPVKA 560 +E + AL G+ PF G SPV A Sbjct: 532 VVLETLLAALQGKAPFAGSSPVDA 555 >ref|NP_687720.1| glycosyl hydrolase, family 3 [Streptococcus agalactiae 2603V/R] gb|AAM99592.1| glycosyl hydrolase, family 3 [Streptococcus agalactiae 2603V/R] Length = 596 Score = 205 bits (521), Expect = 3e-51, Method: Compositional matrix adjust. Identities = 160/593 (26%), Positives = 258/593 (43%), Gaps = 62/593 (10%) Query: 7 LTREEQDWVERTLTSMSLREKIGQTMQDHAGRLPFKGNDEEALAAYLKTYPVGSFFIGGE 66 L +E +W+E+T+ M+L EKIGQ + EE L L Y + + Sbjct: 14 LNQEAIEWIEKTINEMTLDEKIGQLFFNMGA-----SRSEEYLTDVLDRYHIAA------ 62 Query: 67 VIQKAAGKAEEYRDWAEQLQRISKYPLLFSGDLEFGAGSAVKSLTAFPPXXXXXXXXXXX 126 ++ G + E D LQ SK P+L + + E G AV T Sbjct: 63 -VRYNRGSSSEIYDQNLILQTKSKLPMLIAANTEAGGDGAVTDGTKVGDEIKVAATNDPK 121 Query: 127 XXXXXXKFTALEGRAAGFTWALAPGTDLLLNWMNPVITTRCXXXXXXXXXXXXXXVMRGM 186 + +E A G + +P DL NW NP+I +R M+G+ Sbjct: 122 YAYEMGRIAGMEASAVGCNASFSPIVDLTRNWRNPIIASRNWGANVDQIISLSKEYMKGI 181 Query: 187 QDHGLAACAKHFPGDGVDYRDQHIVTTINGLSEEEWFAAYGQVSQHMIDQGVLSYMTGHI 246 + + AKHFPGDG+D RD H+ N +S+EEW + +G++ + D G+ M GHI Sbjct: 182 MQYNIVPFAKHFPGDGIDERDHHLSFASNPMSKEEWMSTFGRIYGELADAGLPGVMAGHI 241 Query: 247 ALPWLEGGTQEEKP----VPATVSERITTGLLRERMGFAGVVLSDALDMGGFLAWGSYEK 302 LP +E E+ +PA++++ + LLR +G+ G +++DA M G A + Sbjct: 242 HLPNVEKEMHPERDLDDMLPASLNKTLLDELLRGELGYNGAIVTDASHMVGMTASMARRD 301 Query: 303 RMVDCFNSGTDVLLWPG--VEYFGVMEQAVSEGTVLMERLDASVRRVLELKALLGVHRVD 360 + +G D+ L+ E M++ +G + ERL ++RR L LKA LG+H + Sbjct: 302 LLPTAIEAGCDLFLFFNDPDEDIQWMKEGYEKGILTEERLHDALRRTLGLKAKLGLHNYE 361 Query: 361 QKGRD-----PQAVPLLNDRLPPKLDLEARQLSRTIAERCITLVRNR-GGILPLDPQTTR 414 + ++ +A+ L+N LE+++++ +A++ +TLV+++ I P++P+ R Sbjct: 362 GRRQELFMPKDKAMALIN-------TLESQKIADEVADKAVTLVKDKQKDIFPVNPERYR 414 Query: 415 RVLVVLLN-------KVTEGRKYERMNVFVELLRARGLQVDILDEFEPLGTLRKWELSGI 467 +L+V + + G K + ELL ARG +V + + E R +L Sbjct: 415 HILLVNVEGYKGGFGAMIAGNKQRASDYMKELLEARGHEVTVWESTEE----RIMKLPQE 470 Query: 468 RWDAAFAPYFLPLHGMMNTARPV------------GEAAKAIWAMQHAETIRP------- 508 AA A + + N G + IW +P Sbjct: 471 ERAAAIANVYAQKQPIANLTEHYDLIINLVDVNAGGTTQRIIWPAAKGTPDQPFYVHEIP 530 Query: 509 -IGISFATPYLLQDMPFLDALINAYSLHEETVELTVKALFGEVPFQGQSPVKA 560 I IS + L DMP + INAY T+ V L GE F G SPV A Sbjct: 531 SIVISVQHAFALADMPQVGTYINAYDGLPSTISAVVAKLAGESEFTGVSPVDA 583 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.320 0.137 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 819,079,958 Number of extensions: 32221591 Number of successful extensions: 76406 Number of sequences better than 1.0e-05: 245 Number of HSP's gapped: 76119 Number of HSP's successfully gapped: 245 Length of query: 577 Length of database: 757,237,578 Length adjustment: 131 Effective length of query: 446 Effective length of database: 401,559,609 Effective search space: 179095585614 Effective search space used: 179095585614 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)