BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0274.0006.p (354 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|AAQ87099.1| Hypothetical protein RNGR00073 [Rhizobium sp. NGR... 176 5e-43 ref|ZP_00037457.1| COG4225: Predicted unsaturated glucuronyl hyd... 171 3e-41 ref|NP_814213.1| conserved hypothetical protein [Enterococcus fa... 170 5e-41 ref|NP_535039.1| conserved hypothetical protein [Agrobacterium t... 169 6e-41 ref|NP_356097.1| AGR_L_618p [Agrobacterium tumefaciens] >gi|2552... 169 8e-41 >gb|AAQ87099.1| Hypothetical protein RNGR00073 [Rhizobium sp. NGR234] Length = 366 Score = 176 bits (447), Expect = 5e-43, Method: Compositional matrix adjust. Identities = 114/327 (34%), Positives = 160/327 (48%), Gaps = 16/327 (4%) Query: 22 EHKGHWGMDIDHWDWVPGVGVISLMEYATASGRGEVLDYLLQWVNRNKRKAEGAKVINAL 81 EH G G+ D WDW GVG+ + A A+G + +D L+ W + + IN+ Sbjct: 38 EHAG--GIQFDEWDWEVGVGLYGFLRRAIATGDRKAIDDLVAWYAWQIDRGLPPRQINSS 95 Query: 82 APFALFPELYRQTGDPWFLQKAMSTADWMLNAAPVTREGALEHTVTENVDFPEQVWADTV 141 AP L P F ADW+++ P T +G +H V E ++ E +W DT+ Sbjct: 96 APMLPLVILTEHVDRPDFRALVEDWADWLVHKLPKTEDGGFQHVVKERLNDGE-LWDDTL 154 Query: 142 YMAVLFLARLGGLTGEPRLADAAVXXXXXXXXXXXDPESGLLYHGWNSRAGNHMSAVHWA 201 +MA LFLAR G + D AV DP +GL YHGW ++ + WA Sbjct: 155 FMACLFLARAGVICERRDWIDEAVYQFMIHTRYLSDPATGLWYHGWTFNGRHNFAKAFWA 214 Query: 202 RANAWITLAVPQIVAELREAVAIPEELYSRYRLLAAGLLS-------CQAGNGLWHTVLN 254 R NAWIT+A+P++ EL +P S R L+ L S Q +G++HT++N Sbjct: 215 RGNAWITVAIPELF-EL-----VPSLNPSDKRFLSNVLASQVRALKRHQRDDGMFHTLVN 268 Query: 255 RPDFVQETSGSAGIACGFIKGVEQGLLESSYLDGARQTVQGILPLIAENGEVQGVSGGTP 314 P ETS +AGIA G ++G+E G+L A + + +L I G VQGVS GTP Sbjct: 269 DPTSPVETSATAGIAYGILRGIEAGILRQGDRVHADRALMAVLGQIDAEGVVQGVSDGTP 328 Query: 315 VMPSADAYNTIEIYPTLYGQGLVLQLF 341 + D Y I PT YGQ L + L Sbjct: 329 MGHDLDFYRRIPNLPTPYGQALTMLLL 355 >ref|ZP_00037457.1| COG4225: Predicted unsaturated glucuronyl hydrolase involved in regulation of bacterial surface properties, and related proteins [Enterococcus faecium] Length = 368 Score = 171 bits (433), Expect = 3e-41, Method: Compositional matrix adjust. Identities = 107/350 (30%), Positives = 164/350 (46%), Gaps = 14/350 (4%) Query: 7 LAETLDLLFRYMI-LEEHKGHWGMDIDH----------WDWVPGVGVISLMEYATASGRG 55 + E +DLL + +++ G + +D D W+W GVG+ + + + Sbjct: 14 VKEKIDLLVENLTTIKDDNGEFLLDFDGLKVDDKSWTVWNWPQGVGLYGIYKNYQMTKNP 73 Query: 56 EVLDYLLQWVNRNKRKAEGAKVINALAPFALFPELYRQTGDPWFLQKAMSTADWMLNAAP 115 + +W + K +N +AP LY T D +L A+W++N P Sbjct: 74 RAYQVVNEWFEDRMEEGAPPKNVNTMAPLLTMAYLYEDTKDSRYLPYLEQWAEWVMNDMP 133 Query: 116 VTREGALEHTVTENVDFPEQVWADTVYMAVLFLARLGGLTGEPRLADAAVXXXXXXXXXX 175 T E L+H T + Q+W DT+ M VL LA++G L P + A Sbjct: 134 RTNEDGLQH-ATYGPENKNQLWDDTLMMTVLPLAKIGKLLNRPEYLEEARYQFMIHIKYL 192 Query: 176 XDPESGLLYHGWNSRAGNHMSAVHWARANAWITLAVPQIVA--ELREAVAIPEELYSRYR 233 D +GL YHGW ++ + WAR N W+T+A+P+I+ EL + A+ + L Sbjct: 193 MDKRTGLWYHGWTFEGNHNYAEAFWARGNCWLTIAIPEIIEILELSKEDALRKLLIETLE 252 Query: 234 LLAAGLLSCQAGNGLWHTVLNRPDFVQETSGSAGIACGFIKGVEQGLLESSYLDGARQTV 293 L + Q+ +GLWHT+L+ P E+S +AG A G +K V + L Y + A + + Sbjct: 253 AQVRALKTYQSESGLWHTLLDDPSSYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAI 312 Query: 294 QGILPLIAENGEVQGVSGGTPVMPSADAYNTIEIYPTLYGQGLVLQLFTQ 343 QG+L I E GEVQ VS GT + S D Y I I YGQ L + T+ Sbjct: 313 QGLLEQIDEKGEVQNVSIGTGMGDSLDFYRNIGITAMPYGQSLTVLCLTE 362 >ref|NP_814213.1| conserved hypothetical protein [Enterococcus faecalis V583] gb|AAO80284.1| conserved hypothetical protein [Enterococcus faecalis V583] Length = 370 Score = 170 bits (430), Expect = 5e-41, Method: Compositional matrix adjust. Identities = 108/347 (31%), Positives = 168/347 (48%), Gaps = 14/347 (4%) Query: 10 TLDLLFRYMI-LEEHKGHWGMDIDH----------WDWVPGVGVISLMEYATASGRGEVL 58 T+DLL R + ++++ G + +D D W+W GVG+ + + + + L Sbjct: 19 TIDLLIRNLTTIKDNTGEFLLDFDGLKVDDKSWTIWNWPQGVGLYGIYKNYRNTKSEKAL 78 Query: 59 DYLLQWVNRNKRKAEGAKVINALAPFALFPELYRQTGDPWFLQKAMSTADWMLNAAPVTR 118 + W ++ K +N +AP LY T D ++ A+W++ P T Sbjct: 79 QVVNDWFEGRMQEGAPPKNVNTMAPLLTMAYLYEDTKDSKYIPYLEQWAEWVMEEMPRTN 138 Query: 119 EGALEHTVTENVDFPEQVWADTVYMAVLFLARLGGLTGEPRLADAAVXXXXXXXXXXXDP 178 EG L+H T + Q+W DT+ M VL LA++G L + A D Sbjct: 139 EGGLQH-ATYGPENKNQLWDDTLMMTVLPLAKIGKLLNRLDYLEEAKHQFLIHIKYLQDK 197 Query: 179 ESGLLYHGWNSRAGNHMSAVHWARANAWITLAVPQIVA--ELREAVAIPEELYSRYRLLA 236 +SGL YHGW ++ + WAR N WIT+A+P+I+ EL + ++ E L S Sbjct: 198 KSGLWYHGWTFEGNHNYAEALWARGNCWITIAIPEIIEILELPKGDSLREFLLSTLNAQV 257 Query: 237 AGLLSCQAGNGLWHTVLNRPDFVQETSGSAGIACGFIKGVEQGLLESSYLDGARQTVQGI 296 A L Q +GLWHT++N + E+S +AG A G +K V + + S Y + A + + G+ Sbjct: 258 AALAKYQDESGLWHTLINDSNSYLESSATAGFAYGILKAVHKKYISSEYEEVANKAIAGL 317 Query: 297 LPLIAENGEVQGVSGGTPVMPSADAYNTIEIYPTLYGQGLVLQLFTQ 343 L I E GEVQ VS GT + + D Y TI + YGQ L + T+ Sbjct: 318 LNEIDETGEVQHVSVGTGMGDNLDFYRTIGMTAMPYGQSLTILCLTE 364 >ref|NP_535039.1| conserved hypothetical protein [Agrobacterium tumefaciens str. C58 (U. Washington)] pir||AE3117 conserved hypothetical protein Atu4561 [imported] - Agrobacterium tumefaciens (strain C58, Dupont) gb|AAL45355.1| conserved hypothetical protein [Agrobacterium tumefaciens str. C58 (U. Washington)] Length = 364 Score = 169 bits (429), Expect = 6e-41, Method: Compositional matrix adjust. Identities = 105/315 (33%), Positives = 156/315 (49%), Gaps = 4/315 (1%) Query: 29 MDIDHWDWVPGVGVISLMEYATASGRGEVLDYLLQWVNRNKRKAEGAKVINALAPFALFP 88 + D WDW GVG+ + A A+ + LD L+ W + + IN+ AP Sbjct: 41 IQFDEWDWEVGVGLYGFLRRALAANDQKALDDLVTWYGWQIDRGLPPRQINSSAPMLPLV 100 Query: 89 ELYRQTGDPWFLQKAMSTADWMLNAAPVTREGALEHTVTENVDFPEQVWADTVYMAVLFL 148 L P F ADW+++ P T +G +H V E ++ E +W DT++MA LFL Sbjct: 101 ILTEHVDRPDFRALVEDWADWLVHELPKTEDGGFQHVVKERLNEGE-LWDDTLFMACLFL 159 Query: 149 ARLGGLTGEPRLADAAVXXXXXXXXXXXDPESGLLYHGWNSRAGNHMSAVHWARANAWIT 208 AR G + D AV DP +GL YHGW ++ + WAR N+WIT Sbjct: 160 ARAGVVCKRQDWIDEAVYQFMIHARYLSDPVTGLWYHGWTFNGRHNFADAFWARGNSWIT 219 Query: 209 LAVPQIVAELREAVAIPEELYSRYRLLAA--GLLSCQAGNGLWHTVLNRPDFVQETSGSA 266 +A+P++ EL +A ++ + L + L Q +G++HT+L+ P ETS +A Sbjct: 220 VAIPELF-ELVPTLAGKDKRFLANVLSSQVRSLRKYQREDGMFHTLLDDPTSPVETSATA 278 Query: 267 GIACGFIKGVEQGLLESSYLDGARQTVQGILPLIAENGEVQGVSGGTPVMPSADAYNTIE 326 GIA G ++G+E G+L A + ++ +L I + G V GVS GTP+ D Y I Sbjct: 279 GIAYGILRGIEAGILGEENRPHAERALKAVLGNIDDEGVVHGVSDGTPMGHDLDFYRRIP 338 Query: 327 IYPTLYGQGLVLQLF 341 PT YGQ L + L Sbjct: 339 NLPTPYGQALTMLLL 353 >ref|NP_356097.1| AGR_L_618p [Agrobacterium tumefaciens] pir||H98169 hypothetical protein AGR_L_618 [imported] - Agrobacterium tumefaciens (strain C58, Cereon) gb|AAK88882.1| AGR_L_618p [Agrobacterium tumefaciens str. C58 (Cereon)] Length = 397 Score = 169 bits (429), Expect = 8e-41, Method: Compositional matrix adjust. Identities = 105/315 (33%), Positives = 156/315 (49%), Gaps = 4/315 (1%) Query: 29 MDIDHWDWVPGVGVISLMEYATASGRGEVLDYLLQWVNRNKRKAEGAKVINALAPFALFP 88 + D WDW GVG+ + A A+ + LD L+ W + + IN+ AP Sbjct: 74 IQFDEWDWEVGVGLYGFLRRALAANDQKALDDLVTWYGWQIDRGLPPRQINSSAPMLPLV 133 Query: 89 ELYRQTGDPWFLQKAMSTADWMLNAAPVTREGALEHTVTENVDFPEQVWADTVYMAVLFL 148 L P F ADW+++ P T +G +H V E ++ E +W DT++MA LFL Sbjct: 134 ILTEHVDRPDFRALVEDWADWLVHELPKTEDGGFQHVVKERLNEGE-LWDDTLFMACLFL 192 Query: 149 ARLGGLTGEPRLADAAVXXXXXXXXXXXDPESGLLYHGWNSRAGNHMSAVHWARANAWIT 208 AR G + D AV DP +GL YHGW ++ + WAR N+WIT Sbjct: 193 ARAGVVCKRQDWIDEAVYQFMIHARYLSDPVTGLWYHGWTFNGRHNFADAFWARGNSWIT 252 Query: 209 LAVPQIVAELREAVAIPEELYSRYRLLAA--GLLSCQAGNGLWHTVLNRPDFVQETSGSA 266 +A+P++ EL +A ++ + L + L Q +G++HT+L+ P ETS +A Sbjct: 253 VAIPELF-ELVPTLAGKDKRFLANVLSSQVRSLRKYQREDGMFHTLLDDPTSPVETSATA 311 Query: 267 GIACGFIKGVEQGLLESSYLDGARQTVQGILPLIAENGEVQGVSGGTPVMPSADAYNTIE 326 GIA G ++G+E G+L A + ++ +L I + G V GVS GTP+ D Y I Sbjct: 312 GIAYGILRGIEAGILGEENRPHAERALKAVLGNIDDEGVVHGVSDGTPMGHDLDFYRRIP 371 Query: 327 IYPTLYGQGLVLQLF 341 PT YGQ L + L Sbjct: 372 NLPTPYGQALTMLLL 386 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.319 0.135 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 524,879,810 Number of extensions: 21339997 Number of successful extensions: 43991 Number of sequences better than 1.0e-05: 40 Number of HSP's gapped: 43933 Number of HSP's successfully gapped: 40 Length of query: 354 Length of database: 757,237,578 Length adjustment: 127 Effective length of query: 227 Effective length of database: 412,420,005 Effective search space: 93619341135 Effective search space used: 93619341135 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 126 (53.1 bits)