BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0273.0005.p (150 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00076658.1| COG0346: Lactoylglutathione lyase and related... 61 7e-09 gb|EAI86177.1| unknown [environmental sequence] 53 2e-06 ref|NP_275900.1| S-D-lactoylglutathione methylglyoxal lyase [Met... 53 3e-06 ref|NP_635242.1| conserved protein [Methanosarcina mazei Goe1] >... 52 3e-06 ref|NP_785963.1| ring-cleaving dioxygenase (putative) [Lactobaci... 52 4e-06 >ref|ZP_00076658.1| COG0346: Lactoylglutathione lyase and related lyases [Methanosarcina barkeri] Length = 138 Score = 61.2 bits (147), Expect = 7e-09, Method: Compositional matrix adjust. Identities = 39/112 (34%), Positives = 58/112 (51%), Gaps = 4/112 (3%) Query: 39 LRVRDLEASLNFYHGILGLPIERRFESRGKQIVMLGTAEQPKVELIQANDQAVKPECGVS 98 L + DL ++L FY +LG P RFE + L A+ + LI +D+A+KP Sbjct: 28 LYINDLNSALEFYEELLGSPAAMRFEIPQ---IGLELAQIDNILLIAGSDEALKPFRSTQ 84 Query: 99 IGFEVKSLDEAIEYLKSRGIPVLRGPVTPNPQLRFFYILDPDGFEVQLAEHS 150 F + SLDE +L+ +G +LRGP P R + +PDG ++ EHS Sbjct: 85 ATFLIDSLDEFKAHLEEKGAEILRGP-NKVPTGRNMTVKNPDGSVIEYVEHS 135 >gb|EAI86177.1| unknown [environmental sequence] Length = 139 Score = 52.8 bits (125), Expect = 2e-06, Method: Compositional matrix adjust. Identities = 41/118 (34%), Positives = 58/118 (49%), Gaps = 13/118 (11%) Query: 37 ITLRVRDLEASLNFYHGILGLPIE---RRFESRGKQ---IVMLGTAEQPKVELIQANDQA 90 I L V+DLE S+ FYH ILG+PI+ R + G+Q I+ L A I + Sbjct: 9 IILEVKDLENSIKFYHEILGMPIKNERRNWVDLGQQSGGILSLHPAS------ITTSHTG 62 Query: 91 VKPECGVSIGFEVKSLDEAIEYLKSRGIPVLRGPVTPNPQLRFFYILDPDGFEVQLAE 148 E G+ IG V L+ A+E L + I + R + + ILDPDG+ + L E Sbjct: 63 PSKENGILIGLTVGDLNSAMEELSAAKIEIFR-DIQERQAGKNAIILDPDGYLISLFE 119 >ref|NP_275900.1| S-D-lactoylglutathione methylglyoxal lyase [Methanothermobacter thermautotrophicus] pir||C69201 S-D-lactoylglutathione methylglyoxal lyase - Methanobacterium thermoautotrophicum (strain Delta H) gb|AAB85261.1| S-D-lactoylglutathione methylglyoxal lyase [Methanothermobacter thermautotrophicus str. Delta H] Length = 116 Score = 52.8 bits (125), Expect = 3e-06, Method: Compositional matrix adjust. Identities = 38/120 (31%), Positives = 63/120 (52%), Gaps = 7/120 (5%) Query: 32 LSLNWITLRVRDLEASLNFYHGILGLPIERRFE--SRGKQIVMLGTAEQPKVELIQANDQ 89 + + + T+ V ++E S+ FY ++G ++ ++ + G+ ++ G E VELI+ + Sbjct: 1 MKIKYATIIVDEMEESVKFYRDVMGFTVDSHYDLGASGEITILRGEGEA-MVELIK--NP 57 Query: 90 AVKPECGVSIGFEVKSLDEAIEYLKSRGIPVLRGPVTPNPQLRFFYILDPDGFEVQLAEH 149 KP SIG EV L+ + L+SRG VL P TP +I DP+G + L EH Sbjct: 58 TDKPGF-FSIGMEVDDLEATLNDLRSRGARVLMEP-TPITVGLLAFIEDPNGVRIALIEH 115 >ref|NP_635242.1| conserved protein [Methanosarcina mazei Goe1] gb|AAM32914.1| conserved protein [Methanosarcina mazei Goe1] Length = 120 Score = 52.4 bits (124), Expect = 3e-06, Method: Compositional matrix adjust. Identities = 36/114 (31%), Positives = 56/114 (49%), Gaps = 8/114 (7%) Query: 39 LRVRDLEASLNFYHGILGLPIERRFE--SRGKQIVMLGTAEQPKVELIQANDQAVKPECG 96 L V DL +L FY +L P+ RFE G ++ + T + LI +++A+KP Sbjct: 10 LYVADLNPALEFYEELLETPVAMRFEIPQTGVELAQIST-----ILLIAGSEEALKPFRN 64 Query: 97 VSIGFEVKSLDEAIEYLKSRGIPVLRGPVTPNPQLRFFYILDPDGFEVQLAEHS 150 F V SLD+ +L+ G ++RGP + P R + DG ++ EHS Sbjct: 65 TQATFLVDSLDKFKTFLEENGAEIIRGP-SKVPTGRNMTVRHSDGSVIEYVEHS 117 >ref|NP_785963.1| ring-cleaving dioxygenase (putative) [Lactobacillus plantarum WCFS1] emb|CAD64814.1| ring-cleaving dioxygenase (putative) [Lactobacillus plantarum WCFS1] Length = 149 Score = 52.0 bits (123), Expect = 4e-06, Method: Compositional matrix adjust. Identities = 40/142 (28%), Positives = 67/142 (47%), Gaps = 16/142 (11%) Query: 18 QNRIFIEGNHKEMILSLNWITLRVRDLEASLNFYHGILGLPI-----ERRFESRGKQIVM 72 N +F++G I ++ +TL V D+ SL FYH + LPI +R+ GKQ + Sbjct: 13 NNELFLKGFELVNIRDIDHLTLTVTDIARSLRFYHEVFDLPIVTFDGDRQAVLVGKQKIN 72 Query: 73 LGTAEQPKVELIQANDQAVKPECGVS--IGFEVKSLDEAIEYLKSRGIPVLRGPVT---P 127 T QP + A KP G + ++D+ +LKS + V+ GPV Sbjct: 73 FQTTNQPHQPI------AAKPTPGSADLCLIAKDNIDDIQHHLKSYFVDVITGPVERTGA 126 Query: 128 NPQLRFFYILDPDGFEVQLAEH 149 + +L Y+ DPD ++++ + Sbjct: 127 HGKLTSLYVRDPDNNLIEISNY 148 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.322 0.142 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 222,487,406 Number of extensions: 8198128 Number of successful extensions: 21822 Number of sequences better than 1.0e-05: 6 Number of HSP's gapped: 21913 Number of HSP's successfully gapped: 6 Length of query: 150 Length of database: 757,237,578 Length adjustment: 109 Effective length of query: 41 Effective length of database: 461,291,787 Effective search space: 18912963267 Effective search space used: 18912963267 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 120 (50.8 bits)