BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0271.0021.p (67 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_654679.1| DUF170, Uncharacterized ACR, COG2104 [Bacillus ... 72 5e-12 ref|NP_830538.1| ThiS protein [Bacillus cereus ATCC 14579] >gi|2... 69 3e-11 ref|ZP_00183341.1| hypothetical protein Exigu127101 [Exiguobacte... 65 6e-10 ref|NP_249071.1| conserved hypothetical protein [Pseudomonas aer... 65 7e-10 ref|ZP_00089135.1| COG2104: Sulfur transfer protein involved in ... 64 1e-09 >ref|NP_654679.1| DUF170, Uncharacterized ACR, COG2104 [Bacillus anthracis A2012] ref|NP_843256.1| conserved hypothetical protein [Bacillus anthracis str. Ames] ref|NP_977125.1| conserved hypothetical protein [Bacillus cereus ATCC 10987] gb|AAP24742.1| conserved hypothetical protein [Bacillus anthracis str. Ames] gb|AAS39733.1| conserved hypothetical protein [Bacillus cereus ATCC 10987] Length = 67 Score = 71.6 bits (174), Expect = 5e-12, Method: Compositional matrix adjust. Identities = 32/67 (47%), Positives = 48/67 (71%) Query: 1 MNLIINGKALEIAEDCRTVADLLSRPEWSGRLVIVERNGEIVNREHYAGTSFSEGDRLEL 60 MNL ING +E+ E +TVA+LL+ E R+V+VERN +I+ ++ + TS +GD++E+ Sbjct: 1 MNLKINGNQIEVPESVKTVAELLTHLELDNRIVVVERNKDILQKDDHTDTSVFDGDQIEI 60 Query: 61 VHFVGGG 67 V FVGGG Sbjct: 61 VTFVGGG 67 >ref|NP_830538.1| ThiS protein [Bacillus cereus ATCC 14579] gb|AAP07739.1| ThiS protein [Bacillus cereus ATCC 14579] Length = 67 Score = 69.3 bits (168), Expect = 3e-11, Method: Compositional matrix adjust. Identities = 31/67 (46%), Positives = 47/67 (70%) Query: 1 MNLIINGKALEIAEDCRTVADLLSRPEWSGRLVIVERNGEIVNREHYAGTSFSEGDRLEL 60 MNL ING +E+ +TVA+LL+ E R+V+VERN +I+ ++ + TS +GD++E+ Sbjct: 1 MNLKINGNQIEVPASVKTVAELLTHLELDNRIVVVERNKDILQKDDHTDTSVFDGDQIEI 60 Query: 61 VHFVGGG 67 V FVGGG Sbjct: 61 VTFVGGG 67 >ref|ZP_00183341.1| hypothetical protein Exigu127101 [Exiguobacterium sp. 255-15] Length = 67 Score = 64.7 bits (156), Expect = 6e-10, Method: Compositional matrix adjust. Identities = 29/67 (43%), Positives = 44/67 (65%) Query: 1 MNLIINGKALEIAEDCRTVADLLSRPEWSGRLVIVERNGEIVNREHYAGTSFSEGDRLEL 60 MN+ +NGK + E+ T+ DLL G++VI+E+N +I++R Y T+ GD +E+ Sbjct: 1 MNIRLNGKDYALIEETTTLHDLLQHLAIDGKIVIIEQNRQIIDRSRYPDTTVQPGDVVEI 60 Query: 61 VHFVGGG 67 VHFVGGG Sbjct: 61 VHFVGGG 67 >ref|NP_249071.1| conserved hypothetical protein [Pseudomonas aeruginosa PA01] ref|ZP_00140816.1| COG2104: Sulfur transfer protein involved in thiamine biosynthesis [Pseudomonas aeruginosa UCBPP-PA14] pir||D83599 conserved hypothetical protein PA0380 [imported] - Pseudomonas aeruginosa (strain PAO1) gb|AAG03769.1| conserved hypothetical protein [Pseudomonas aeruginosa PAO1] Length = 66 Score = 64.7 bits (156), Expect = 7e-10, Method: Compositional matrix adjust. Identities = 32/67 (47%), Positives = 46/67 (68%), Gaps = 1/67 (1%) Query: 1 MNLIINGKALEIAEDCRTVADLLSRPEWSGRLVIVERNGEIVNREHYAGTSFSEGDRLEL 60 M + +NG+ E+ D ++VADLL+R E GR V VE N +IV R +A T+ +GDR+E+ Sbjct: 1 MQIQLNGEPFELP-DAQSVADLLARLELGGRRVAVELNLDIVPRSQHASTALKDGDRVEI 59 Query: 61 VHFVGGG 67 VH +GGG Sbjct: 60 VHAIGGG 66 >ref|ZP_00089135.1| COG2104: Sulfur transfer protein involved in thiamine biosynthesis [Azotobacter vinelandii] Length = 66 Score = 63.5 bits (153), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 33/67 (49%), Positives = 48/67 (71%), Gaps = 1/67 (1%) Query: 1 MNLIINGKALEIAEDCRTVADLLSRPEWSGRLVIVERNGEIVNREHYAGTSFSEGDRLEL 60 M + +NG+ E+A D +T+ADLL+R + +GR V VE N +IV R +A T+ EGDR+E+ Sbjct: 1 MRIQLNGEPYELA-DGQTIADLLARLDLAGRRVAVELNLDIVPRSLHAATALREGDRVEV 59 Query: 61 VHFVGGG 67 VH +GGG Sbjct: 60 VHAIGGG 66 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.319 0.140 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 99,959,579 Number of extensions: 3137784 Number of successful extensions: 7085 Number of sequences better than 1.0e-05: 24 Number of HSP's gapped: 7111 Number of HSP's successfully gapped: 24 Length of query: 67 Length of database: 757,237,578 Length adjustment: 38 Effective length of query: 29 Effective length of database: 654,063,816 Effective search space: 18967850664 Effective search space used: 18967850664 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 120 (50.8 bits)