BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0271.0019.p (370 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00061449.1| COG1060: Thiamine biosynthesis enzyme ThiH an... 422 e-117 ref|NP_349523.1| Thiamine biosynthesis enzyme, thiH [Clostridium... 388 e-106 ref|ZP_00130167.1| COG1060: Thiamine biosynthesis enzyme ThiH an... 377 e-103 ref|NP_562516.1| thiamin biosynthesis protein [Clostridium perfr... 369 e-101 ref|NP_602569.1| ThiH protein [Fusobacterium nucleatum subsp. nu... 309 6e-83 >ref|ZP_00061449.1| COG1060: Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes [Clostridium thermocellum ATCC 27405] Length = 369 Score = 422 bits (1086), Expect = e-117, Method: Compositional matrix adjust. Identities = 206/366 (56%), Positives = 261/366 (71%), Gaps = 3/366 (0%) Query: 1 MSFYESLTRLEQLPYESLWQQFQVEDVKRALCKDR-LDESDLLALLSPAAEPCLEEIARR 59 MSFYE + +E+ + Q D+ + KDR L E + L LLS A LE +A++ Sbjct: 1 MSFYERYLEYKNFDFENFFDQVTDRDIINIINKDRRLSELEFLMLLSKKAVKYLEPLAQK 60 Query: 60 AQRLTRTHFGHVMQLFTPMYLADFCVNHCTYCSFSSIYDFPRKRLTPEEVRQEAIAIAST 119 A R+T +FG V+ L+TPMYLA++CVN C YC F+ + R++LT +EV +EA AI+ST Sbjct: 61 ANRITVQNFGKVIFLYTPMYLANYCVNQCIYCGFNITNNIKRRKLTLDEVEKEAYAISST 120 Query: 120 GLRHLLVLTGESRSASPASYVKECVEILHEYFPSVSIEVNPLSTAEYRELREAGVDGLTL 179 GLRH+L+LTGESR SP Y+K+CV+IL +YF S+SIEV PL EY EL EAGVDGLT+ Sbjct: 121 GLRHILILTGESRKESPVQYIKDCVKILQKYFRSISIEVYPLEENEYAELIEAGVDGLTI 180 Query: 180 YQEVYHQETYRKLHVKGPKRVFRNRLDAPERGCQAGFRAVNIGALLGMYDWRQEAFMTAM 239 YQEVY +E Y+ LH+KGPKR + RLDAPER C+A R VNIGALLG++DWR EAF T + Sbjct: 181 YQEVYDEEKYKALHLKGPKRNYLYRLDAPERACKASMRNVNIGALLGLHDWRTEAFYTGL 240 Query: 240 HARYLQDKYPEXXXXLSAPRFRPY-LGEFNPDSDVTDRALVQIILAYRLFLPRSGITLST 298 HA YLQ+KYP+ LS PR RP+ G F PD V DR LVQI++AYRLF+PR+GI +ST Sbjct: 241 HADYLQNKYPDVEIGLSLPRIRPHPCGSFVPDCKVEDRDLVQIMIAYRLFMPRAGIAIST 300 Query: 299 REPAPLRNHLIHLGITKMSAGVSTEVGGHT-QDGGTPQFEISDERSVEEITDMLQANGLQ 357 RE LRN+LI LG+TKMSA STEVGGHT D QF+++D R VEE+ M+ + G Q Sbjct: 301 RERESLRNNLIGLGVTKMSAASSTEVGGHTLGDKSDGQFDVNDRRGVEEMRQMIYSKGYQ 360 Query: 358 PVFKDW 363 PVFKDW Sbjct: 361 PVFKDW 366 >ref|NP_349523.1| Thiamine biosynthesis enzyme, thiH [Clostridium acetobutylicum] pir||D97259 thiamine biosynthesis enzyme, thiH [imported] - Clostridium acetobutylicum gb|AAK80863.1| Thiamine biosynthesis enzyme, thiH [Clostridium acetobutylicum] Length = 368 Score = 388 bits (996), Expect = e-106, Method: Compositional matrix adjust. Identities = 179/365 (49%), Positives = 261/365 (71%), Gaps = 1/365 (0%) Query: 1 MSFYESLTRLEQLPYESLWQQFQVEDVKRALCKDRLDESDLLALLSPAAEPCLEEIARRA 60 MSFY+ L + + ++ + + D+++ LCKD L E D L LLSPAAE LE +A++A Sbjct: 1 MSFYKKLQQYKDFDFDDFFSKVTARDIEKILCKDILHEMDFLKLLSPAAEKYLEHMAQKA 60 Query: 61 QRLTRTHFGHVMQLFTPMYLADFCVNHCTYCSFSSIYDFPRKRLTPEEVRQEAIAIASTG 120 + L+ +FG + L+TP+Y+A++CVN C YC ++ RK+LT EE+ +EA AI S+G Sbjct: 61 RELSLKNFGKTVVLYTPIYIANYCVNGCAYCGYNVKNKIKRKQLTMEEIEEEARAIYSSG 120 Query: 121 LRHLLVLTGESRSASPASYVKECVEILHEYFPSVSIEVNPLSTAEYRELREAGVDGLTLY 180 +R++++LTGES+ +P SY+K+ +++L +YF S+ IE+ PL EYREL EAG D LT+Y Sbjct: 121 MRNIILLTGESKVQTPVSYIKDAIKLLKKYFSSICIEIYPLEVNEYRELVEAGADSLTIY 180 Query: 181 QEVYHQETYRKLHVKGPKRVFRNRLDAPERGCQAGFRAVNIGALLGMYDWRQEAFMTAMH 240 QE Y++E Y K+H+ GPKR F+ RLDAPER C+AG ++ GALLG+Y WR EAF T +H Sbjct: 181 QETYNEEKYSKVHLSGPKRNFKFRLDAPERVCEAGIHSIGTGALLGLYKWRSEAFFTGLH 240 Query: 241 ARYLQDKYPEXXXXLSAPRFRPYLGEFNPDSDVTDRALVQIILAYRLFLPRSGITLSTRE 300 A Y+Q+K+P +SAPR RP+ G F+ +V D+ +VQ+ILAY++FLPR+G ++TRE Sbjct: 241 ASYIQEKFPSVEISMSAPRIRPHAGSFDDIYEVNDKNIVQVILAYKMFLPRAGTNITTRE 300 Query: 301 PAPLRNHLIHLGITKMSAGVSTEVGGH-TQDGGTPQFEISDERSVEEITDMLQANGLQPV 359 P R+ LI +G+TKMSAGVSTEVGGH +D G QF+ +D+RSV E+ + ++ G PV Sbjct: 301 PKEFRDKLIPIGVTKMSAGVSTEVGGHGCKDKGEGQFDTNDKRSVSEVYNRIKELGYNPV 360 Query: 360 FKDWD 364 FKD++ Sbjct: 361 FKDFE 365 >ref|ZP_00130167.1| COG1060: Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes [Desulfovibrio desulfuricans G20] Length = 401 Score = 377 bits (968), Expect = e-103, Method: Compositional matrix adjust. Identities = 187/364 (51%), Positives = 245/364 (67%), Gaps = 2/364 (0%) Query: 2 SFYESLTRLEQLPYESLWQQFQVEDVKRALCKDRLDESDLLALLSPAAEPCLEEIARRAQ 61 FYE + +L+ + ++ V++AL KD L D LALLSPAA P +E +A +A+ Sbjct: 29 GFYEVVRQLQDVDVQAACMAADGYRVRQALAKDTLSPEDFLALLSPAARPYMESMAHKAR 88 Query: 62 RLTRTHFGHVMQLFTPMYLADFCVNHCTYCSFSSIYDFPRKRLTPEEVRQEAIAIASTGL 121 +T FG +QLFTP+YL+++C N C YCSF++ R +L V +E AIA+TGL Sbjct: 89 DVTVRQFGRTIQLFTPLYLSNWCTNRCVYCSFNACSGIDRMQLDAAGVLREGQAIAATGL 148 Query: 122 RHLLVLTGESRSASPASYVKECVEILHEYFPSVSIEVNPLSTAEYRELREAGVDGLTLYQ 181 RHLL+LTGE+ + + Y+++CV +L FPS+SIEV L+ EYR L AGVDG+TL+Q Sbjct: 149 RHLLLLTGEAPAKASVDYIRDCVRVLRPLFPSLSIEVYALTEPEYRTLAVAGVDGMTLFQ 208 Query: 182 EVYHQETYRKLHVKGPKRVFRNRLDAPERGCQAGFRAVNIGALLGMYDWRQEAFMTAMHA 241 E Y++ Y LH GPK + RL APER C+AG R VNIGALLG+ W+++AFMT MHA Sbjct: 209 ETYNEALYPSLHPAGPKSNYHFRLGAPERACRAGMRNVNIGALLGLDQWQRDAFMTGMHA 268 Query: 242 RYLQDKYPEXXXXLSAPRFRPYLGEFNPDSDVTDRALVQIILAYRLFLPRSGITLSTREP 301 +LQ +YP +S PR RPY G F P DV DRALVQI+LA RLFLPR GIT+STRE Sbjct: 269 LWLQHRYPGVDIAVSLPRMRPYAGSFQPVCDVDDRALVQILLAMRLFLPRCGITISTRER 328 Query: 302 APLRNHLIHLGITKMSAGVSTEVGGHTQD--GGTPQFEISDERSVEEITDMLQANGLQPV 359 R++LI LG+T+MSAGVST VGGH ++ QFEISD RSV+E+ + + G Q V Sbjct: 329 PAFRDNLIPLGVTRMSAGVSTAVGGHAENEHSSVGQFEISDPRSVDEVVEAVSRAGYQAV 388 Query: 360 FKDW 363 FKDW Sbjct: 389 FKDW 392 >ref|NP_562516.1| thiamin biosynthesis protein [Clostridium perfringens] dbj|BAB81306.1| thiamin biosynthesis protein [Clostridium perfringens str. 13] Length = 367 Score = 369 bits (946), Expect = e-101, Method: Compositional matrix adjust. Identities = 168/364 (46%), Positives = 252/364 (69%), Gaps = 1/364 (0%) Query: 1 MSFYESLTRLEQLPYESLWQQFQVEDVKRALCKDRLDESDLLALLSPAAEPCLEEIARRA 60 MSFY+ + + + + + EDV R++ + DLL L+SP E LEE+A+ A Sbjct: 1 MSFYDVVEKYRDFDFYGYFDSVKKEDVLRSIYERNKRPEDLLNLISPMGEELLEEMAQEA 60 Query: 61 QRLTRTHFGHVMQLFTPMYLADFCVNHCTYCSFSSIYDFPRKRLTPEEVRQEAIAIASTG 120 + L+ +FG + L+TPMY++++CVN C+YC ++ RK+L EE+ +E AI+ G Sbjct: 61 RNLSLKYFGRTILLYTPMYISNYCVNKCSYCGYNVENKICRKKLNQEEIEKEGEAISKEG 120 Query: 121 LRHLLVLTGESRSASPASYVKECVEILHEYFPSVSIEVNPLSTAEYRELREAGVDGLTLY 180 +H+L+LTGES +P Y++E ++ L E FPS++IE+ P++ Y+++ EAG +GLT+Y Sbjct: 121 FKHILILTGESEYHTPVEYIEESIKTLKEKFPSITIEIYPMTEEGYKKVVEAGAEGLTVY 180 Query: 181 QEVYHQETYRKLHVKGPKRVFRNRLDAPERGCQAGFRAVNIGALLGMYDWRQEAFMTAMH 240 QE Y ++ Y ++HV GPK+ ++ RL+APERG +AG R+++IGALLG+ D+R +AF TAMH Sbjct: 181 QETYDEKVYDRVHVAGPKKNYKFRLEAPERGAEAGMRSISIGALLGLADFRIDAFFTAMH 240 Query: 241 ARYLQDKYPEXXXXLSAPRFRPYLGEFNPDSDVTDRALVQIILAYRLFLPRSGITLSTRE 300 +YL+DKYP S PR RP G ++V DR LVQI+LAYRLF P+ GI +STRE Sbjct: 241 GKYLRDKYPHIDISYSVPRIRPCEGGLKKLNEVDDRELVQILLAYRLFDPQGGINISTRE 300 Query: 301 PAPLRNHLIHLGITKMSAGVSTEVGGHT-QDGGTPQFEISDERSVEEITDMLQANGLQPV 359 R +LI LG++K+SAGVSTEVGGH+ ++ GT QF+I+DE SV E+ +++++ G QP+ Sbjct: 301 GKDFRRNLIPLGVSKISAGVSTEVGGHSLKEKGTSQFDINDESSVSEVKELIKSQGYQPI 360 Query: 360 FKDW 363 FKDW Sbjct: 361 FKDW 364 >ref|NP_602569.1| ThiH protein [Fusobacterium nucleatum subsp. nucleatum ATCC 25586] gb|AAL93868.1| ThiH protein [Fusobacterium nucleatum subsp. nucleatum ATCC 25586] Length = 376 Score = 309 bits (792), Expect = 6e-83, Method: Compositional matrix adjust. Identities = 149/349 (42%), Positives = 219/349 (62%), Gaps = 4/349 (1%) Query: 19 WQQFQVEDVKRALCKDRLDESDLLALLSPAAEPCLEEIARRAQRLTRTHFGHVMQLFTPM 78 + F EDV+ AL KD L D ALLSP A LEE A +A+ +FG+ + +FTP+ Sbjct: 23 YNSFSNEDVEEALNKDYLSVKDFQALLSPKAINYLEEKAEKAKEFKERYFGNSVYIFTPL 82 Query: 79 YLADFCVNHCTYCSFSSIYDFPRKRLTPEEVRQEAIAIASTGLRHLLVLTGESRSASPAS 138 Y++++C N+C YC F+S R +L E++ QE IA +GL+ +L+LTGES S Sbjct: 83 YISNYCDNYCVYCGFNSHNKIKRAKLDFEQIEQELKEIAKSGLQEILILTGESERYSNIE 142 Query: 139 YVKECVEILHEYFPSVSIEVNPLSTAEYRELREAGVDGLTLYQEVYHQETYRKLHVKGPK 198 Y+ E ++ +YF +V IE+ P++ +Y+ L G D +T++QE Y+ E Y+KLH++G K Sbjct: 143 YIGEACKLARKYFNNVGIEIYPVNVEDYKYLNSCGADYVTIFQETYNNEKYKKLHLEGHK 202 Query: 199 RVFRNRLDAPERGCQAGFRAVNIGALLGMYDWRQEAFMTAMHARYLQDKYPEXXXXLSAP 258 +VF R ++ ER G R V GALLG+ D+R++AF T HA LQ KYP +S P Sbjct: 203 KVFSYRFNSQERALMGGMRGVAFGALLGLDDFRKDAFSTGYHAYLLQKKYPYAEISISCP 262 Query: 259 RFRPYLGEFNPDSD-VTDRALVQIILAYRLFLPRSGITLSTREPAPLRNHLIHLGITKMS 317 R RP + + + VT+R L QI+ AYRLFLP + IT+STRE + R+++I + TK+S Sbjct: 263 RLRPVINNLKVEKEIVTERELFQIVCAYRLFLPFANITISTRENSIFRDNIIKIAATKIS 322 Query: 318 AGVSTEVGGHTQ---DGGTPQFEISDERSVEEITDMLQANGLQPVFKDW 363 AGV T +G HT+ G QFEI+D+R+V EI + +++ LQPV D+ Sbjct: 323 AGVDTGIGAHTECSTKKGDEQFEIADKRTVAEIFEKVKSEDLQPVMNDY 371 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.321 0.136 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 536,484,246 Number of extensions: 21585904 Number of successful extensions: 44389 Number of sequences better than 1.0e-05: 70 Number of HSP's gapped: 44299 Number of HSP's successfully gapped: 70 Length of query: 370 Length of database: 757,237,578 Length adjustment: 127 Effective length of query: 243 Effective length of database: 412,420,005 Effective search space: 100218061215 Effective search space used: 100218061215 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 127 (53.5 bits)