BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0271.0017.p (557 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_241760.1| fumarate hydratase class I (fumarase) [Bacillus... 497 e-139 sp|Q04718|FUMA_BACST Fumarate hydratase class I, aerobic (Fumara... 472 e-132 ref|NP_976866.1| fumarate hydratase, class I [Bacillus cereus AT... 449 e-124 ref|NP_830305.1| Fumarate hydratase [Bacillus cereus ATCC 14579]... 447 e-124 ref|NP_654360.1| hypothetical protein predicted by GeneMark [Bac... 446 e-124 >ref|NP_241760.1| fumarate hydratase class I (fumarase) [Bacillus halodurans] pir||F83761 fumarate hydratase class I (fumarase) BH0894 [imported] - Bacillus halodurans (strain C-125) dbj|BAB04613.1| fumarate hydratase class I (fumarase) [Bacillus halodurans] Length = 512 Score = 497 bits (1280), Expect = e-139, Method: Compositional matrix adjust. Identities = 232/299 (77%), Positives = 264/299 (88%) Query: 1 MQHFEDSVYDLIVETSTNLPGDVRRAVAKGRALEDRATRSGLALTTIAQNIGMAELQVSP 60 M+ ++S+Y LIVETSTNLP DVRRA+A+ +A E+ TR+ L+L+TI +NI MA+ +SP Sbjct: 1 MEKLQESMYQLIVETSTNLPNDVRRAIAEAKAKENAGTRAALSLSTITENIKMADENISP 60 Query: 61 ICQDTGMPTFIIHTPVGVNQIEMKKDIHNAIIRATINGKLRPNSVDSLTGENSGNNLGAG 120 ICQDTGMPTF I PVGVNQIEMKK I+ AI +AT +GKLRPNSVDSLTG+NSGNNLG G Sbjct: 61 ICQDTGMPTFEIKVPVGVNQIEMKKAIYAAIEQATKDGKLRPNSVDSLTGKNSGNNLGEG 120 Query: 121 TPVIHFEQWEETGVDVRLILKGGGCENKNIQYSLPAELEGLGKAGRDLDGIRKCILHSVY 180 TPVIHFEQWEE +D RLILKGGGCENKNIQYSLPAELEGLG+AGRDLDGIRKCI+HSVY Sbjct: 121 TPVIHFEQWEEDYIDARLILKGGGCENKNIQYSLPAELEGLGRAGRDLDGIRKCIMHSVY 180 Query: 181 QAQGQGCSAGFIGVGIGGDRTTGYELAKKQLFREVEDVNPNDDLAKLEEYIMENANKLGI 240 QAQGQGCSAGFIGVGIGGDRTT Y LAK+QLFR+V+DVNPNDDL KLEEY++ENANKLGI Sbjct: 181 QAQGQGCSAGFIGVGIGGDRTTSYSLAKEQLFRKVDDVNPNDDLRKLEEYVLENANKLGI 240 Query: 241 GTMGFGGEVTLLGCKIGVMNRLPASFFVSVAYNCWAFRRQGILVDPSTGHIQEWLYESG 299 GTMGFGGE TLLGCK+G MNRLPASFFVSVAYNCWAFRR G+ +D TG I++WLY+ G Sbjct: 241 GTMGFGGEATLLGCKVGAMNRLPASFFVSVAYNCWAFRRLGVELDAETGEIKKWLYKDG 299 Score = 330 bits (846), Expect = 5e-89, Method: Compositional matrix adjust. Identities = 154/192 (80%), Positives = 173/192 (90%), Gaps = 1/192 (0%) Query: 366 REVRLTTPVSEEDIRSLRVGDVVILSGEMHTGRDALHKYLMDHEAPVDLNGAVIYHCGPV 425 REV L PV+EE IR L+VGDVVI++G +HTGRDA+H +LMDH+AP+DL+G +IYHCGPV Sbjct: 321 REVVLEAPVTEEQIRELKVGDVVIINGMLHTGRDAIHHHLMDHDAPIDLDGQIIYHCGPV 380 Query: 426 MLKDEEG-WHVKAAGPTTSIREEPYQGDIIKKFGIRAVIGKGGMGPKTLKALQEHGGVYL 484 ML+D+ G WHVKAAGPTTSIREEPYQGDI+KKFGIRAVIGKGGMGPKTLKALQEHGGVYL Sbjct: 381 MLQDDAGEWHVKAAGPTTSIREEPYQGDIMKKFGIRAVIGKGGMGPKTLKALQEHGGVYL 440 Query: 485 NAIGGAAQYYAECIKKVNGVDFMEFGIPEAMWHLQVDGFAAIVTMDAHGNSLHADVEKDS 544 NAIGGAAQYYA+CIKKV GVD MEFGIPEAMWHL+V+GFAAIVTMD+HGNSLH DV+K S Sbjct: 441 NAIGGAAQYYADCIKKVKGVDLMEFGIPEAMWHLEVEGFAAIVTMDSHGNSLHEDVDKSS 500 Query: 545 AAKLAQFREPVF 556 KL QF E VF Sbjct: 501 LEKLEQFAEKVF 512 >sp|Q04718|FUMA_BACST Fumarate hydratase class I, aerobic (Fumarase) pir||A47692 fumarate hydratase (EC 4.2.1.2), iron-dependent - Bacillus stearothermophilus gb|AAA72317.1| fumarase Length = 514 Score = 472 bits (1215), Expect = e-132, Method: Compositional matrix adjust. Identities = 219/302 (72%), Positives = 259/302 (85%) Query: 1 MQHFEDSVYDLIVETSTNLPGDVRRAVAKGRALEDRATRSGLALTTIAQNIGMAELQVSP 60 M+ +S+Y LIVETST LP DVRRA+ K + E+ TR+ ++L TI +NI MA+ VSP Sbjct: 1 MEKLYESMYQLIVETSTKLPKDVRRAILKAKQRENAGTRAAMSLATITENIKMADENVSP 60 Query: 61 ICQDTGMPTFIIHTPVGVNQIEMKKDIHNAIIRATINGKLRPNSVDSLTGENSGNNLGAG 120 ICQDTG+PTF I PVG+NQI++K+ I AI +AT +GKLRPNSVDSLTGENSG+NLG G Sbjct: 61 ICQDTGLPTFKIKVPVGINQIQIKETIKKAIAQATKDGKLRPNSVDSLTGENSGDNLGEG 120 Query: 121 TPVIHFEQWEETGVDVRLILKGGGCENKNIQYSLPAELEGLGKAGRDLDGIRKCILHSVY 180 PV+ FEQWE+ +DVRLILKGGGCENKNIQYSLP ELEGLG+AGRDLDGIRKCILH+VY Sbjct: 121 LPVVKFEQWEKDYMDVRLILKGGGCENKNIQYSLPCELEGLGRAGRDLDGIRKCILHAVY 180 Query: 181 QAQGQGCSAGFIGVGIGGDRTTGYELAKKQLFREVEDVNPNDDLAKLEEYIMENANKLGI 240 QAQGQGCSAGFIGVGIGGDR+ GY+LAK+QLFREV+DVNPN++L +LEEYIMENANKLGI Sbjct: 181 QAQGQGCSAGFIGVGIGGDRSAGYDLAKEQLFREVDDVNPNEELRQLEEYIMENANKLGI 240 Query: 241 GTMGFGGEVTLLGCKIGVMNRLPASFFVSVAYNCWAFRRQGILVDPSTGHIQEWLYESGT 300 GTMGFGGE TLLGCK+G M+R+PASFFVSVAYNCWAFRR G+ +DP+TG I +WLY+ G Sbjct: 241 GTMGFGGETTLLGCKVGAMHRIPASFFVSVAYNCWAFRRLGVHIDPNTGEIIKWLYQDGE 300 Query: 301 GI 302 + Sbjct: 301 DV 302 Score = 315 bits (807), Expect = 2e-84, Method: Compositional matrix adjust. Identities = 145/192 (75%), Positives = 169/192 (88%), Gaps = 1/192 (0%) Query: 366 REVRLTTPVSEEDIRSLRVGDVVILSGEMHTGRDALHKYLMDHEAPVDLNGAVIYHCGPV 425 R + L P++EE IR L+VGDVV ++G ++TGRDA+HKYLMDH+APVDLNG +IYHCGPV Sbjct: 322 RVITLQAPITEEQIRELKVGDVVRINGIIYTGRDAIHKYLMDHDAPVDLNGQIIYHCGPV 381 Query: 426 MLKDEEG-WHVKAAGPTTSIREEPYQGDIIKKFGIRAVIGKGGMGPKTLKALQEHGGVYL 484 MLKDE G W VKAAGPTTSIREEPYQGDI+KKFGIRAV+GKGGMG KTL+AL+EHGGVYL Sbjct: 382 MLKDENGNWEVKAAGPTTSIREEPYQGDIMKKFGIRAVMGKGGMGQKTLQALKEHGGVYL 441 Query: 485 NAIGGAAQYYAECIKKVNGVDFMEFGIPEAMWHLQVDGFAAIVTMDAHGNSLHADVEKDS 544 NAIGGAAQYYA+CI+ V GVD +EFGIPEAMWHL+V F A+VTMD+HGNSLH D+EK S Sbjct: 442 NAIGGAAQYYADCIEAVEGVDLLEFGIPEAMWHLRVKDFTAVVTMDSHGNSLHEDIEKSS 501 Query: 545 AAKLAQFREPVF 556 KL+QF+EPVF Sbjct: 502 LEKLSQFKEPVF 513 >ref|NP_976866.1| fumarate hydratase, class I [Bacillus cereus ATCC 10987] gb|AAS39474.1| fumarate hydratase, class I [Bacillus cereus ATCC 10987] Length = 506 Score = 449 bits (1154), Expect = e-124, Method: Compositional matrix adjust. Identities = 217/299 (72%), Positives = 256/299 (85%) Query: 1 MQHFEDSVYDLIVETSTNLPGDVRRAVAKGRALEDRATRSGLALTTIAQNIGMAELQVSP 60 M+ ++S+Y LIVETSTNLP DVRRA+ + + E+ TRS +AL TI NI MA+ +SP Sbjct: 1 MEKLQESMYQLIVETSTNLPKDVRRAIQQAKERENAGTRSAMALGTITNNIKMADDNISP 60 Query: 61 ICQDTGMPTFIIHTPVGVNQIEMKKDIHNAIIRATINGKLRPNSVDSLTGENSGNNLGAG 120 ICQDTGMPTF I+TPVGVNQ+++K+ I+NA+ RAT +GKLRPNSVDSL G+NSGNNLG G Sbjct: 61 ICQDTGMPTFKIYTPVGVNQLKLKEAIYNALERATKDGKLRPNSVDSLFGDNSGNNLGPG 120 Query: 121 TPVIHFEQWEETGVDVRLILKGGGCENKNIQYSLPAELEGLGKAGRDLDGIRKCILHSVY 180 TPVI FEQWE+ +D RLILKGGGCENKNIQYSLP ELEGLG+AGRDL+GIRKC+LH+VY Sbjct: 121 TPVIKFEQWEKDYIDARLILKGGGCENKNIQYSLPCELEGLGRAGRDLEGIRKCLLHAVY 180 Query: 181 QAQGQGCSAGFIGVGIGGDRTTGYELAKKQLFREVEDVNPNDDLAKLEEYIMENANKLGI 240 QAQGQGCSAG IGVGIGGDRT+GYELAK QLFR ++DVNP +L +LEEY++ENANKLGI Sbjct: 181 QAQGQGCSAGVIGVGIGGDRTSGYELAKNQLFRTLDDVNPIPELQQLEEYVLENANKLGI 240 Query: 241 GTMGFGGEVTLLGCKIGVMNRLPASFFVSVAYNCWAFRRQGILVDPSTGHIQEWLYESG 299 GTMGFGGE TLLGCKIGV NRLPASF+VSVAYNCWA+RR G+ + P TG I +WLY+ G Sbjct: 241 GTMGFGGETTLLGCKIGVYNRLPASFYVSVAYNCWAYRRLGVTIHPETGEIMDWLYQEG 299 Score = 320 bits (819), Expect = 7e-86, Method: Compositional matrix adjust. Identities = 146/194 (75%), Positives = 172/194 (88%), Gaps = 1/194 (0%) Query: 365 SREVRLTTPVSEEDIRSLRVGDVVILSGEMHTGRDALHKYLMDHEAPVDLNGAVIYHCGP 424 RE+ L P++EE IR LRVGDVV ++G M+TGRDA+HK+LMD++ PVDLNG +IYHCGP Sbjct: 313 QREIVLQAPITEEQIRELRVGDVVTINGMMYTGRDAIHKHLMDNDCPVDLNGQIIYHCGP 372 Query: 425 VMLKDE-EGWHVKAAGPTTSIREEPYQGDIIKKFGIRAVIGKGGMGPKTLKALQEHGGVY 483 V++KDE + W +KAAGPTTSIREEPYQGDI+KKFGIRAVIGKGGMG KTL AL+EHGGVY Sbjct: 373 VVVKDENDNWQIKAAGPTTSIREEPYQGDIMKKFGIRAVIGKGGMGAKTLAALEEHGGVY 432 Query: 484 LNAIGGAAQYYAECIKKVNGVDFMEFGIPEAMWHLQVDGFAAIVTMDAHGNSLHADVEKD 543 LNAIGGAAQYYAECIK+V VDF++FGIPEAMWHL++DGF A+VTMD+HGNSLHADV+K Sbjct: 433 LNAIGGAAQYYAECIKEVKDVDFLQFGIPEAMWHLRIDGFKAVVTMDSHGNSLHADVDKT 492 Query: 544 SAAKLAQFREPVFK 557 S KLA F+EPVFK Sbjct: 493 SLEKLASFKEPVFK 506 >ref|NP_830305.1| Fumarate hydratase [Bacillus cereus ATCC 14579] gb|AAP07506.1| Fumarate hydratase [Bacillus cereus ATCC 14579] Length = 506 Score = 447 bits (1149), Expect = e-124, Method: Compositional matrix adjust. Identities = 217/299 (72%), Positives = 255/299 (85%) Query: 1 MQHFEDSVYDLIVETSTNLPGDVRRAVAKGRALEDRATRSGLALTTIAQNIGMAELQVSP 60 M+ ++S+Y LIVETSTNLP DVRRA+ + + E+ TRS +AL TI NI MA+ +SP Sbjct: 1 MEKLQESMYQLIVETSTNLPKDVRRAIQQAKERENAGTRSAMALGTITNNIKMADDNISP 60 Query: 61 ICQDTGMPTFIIHTPVGVNQIEMKKDIHNAIIRATINGKLRPNSVDSLTGENSGNNLGAG 120 ICQDTGMPTF I+TPVGVNQ+++K+ I+NA+ RAT +GKLRPNSVDSL G+NSGNNLG G Sbjct: 61 ICQDTGMPTFKIYTPVGVNQLKLKEAIYNALERATKDGKLRPNSVDSLFGDNSGNNLGPG 120 Query: 121 TPVIHFEQWEETGVDVRLILKGGGCENKNIQYSLPAELEGLGKAGRDLDGIRKCILHSVY 180 TPVI FEQWE+ +D RLILKGGGCENKNIQYSLP ELEGLG+AGRDL+GIRKC+LH+VY Sbjct: 121 TPVIKFEQWEKDYIDARLILKGGGCENKNIQYSLPCELEGLGRAGRDLEGIRKCLLHAVY 180 Query: 181 QAQGQGCSAGFIGVGIGGDRTTGYELAKKQLFREVEDVNPNDDLAKLEEYIMENANKLGI 240 QAQGQGCSAG IGVGIGGDRT+GYELAK QLFR ++DVNP +L LEEY++ENANKLGI Sbjct: 181 QAQGQGCSAGVIGVGIGGDRTSGYELAKNQLFRTLDDVNPVPELQHLEEYVLENANKLGI 240 Query: 241 GTMGFGGEVTLLGCKIGVMNRLPASFFVSVAYNCWAFRRQGILVDPSTGHIQEWLYESG 299 GTMGFGGE TLLGCKIGV NRLPASF+VSVAYNCWA+RR G+ + P TG I +WLY+ G Sbjct: 241 GTMGFGGETTLLGCKIGVYNRLPASFYVSVAYNCWAYRRLGVKIHPETGDIMDWLYQEG 299 Score = 319 bits (818), Expect = 1e-85, Method: Compositional matrix adjust. Identities = 146/194 (75%), Positives = 172/194 (88%), Gaps = 1/194 (0%) Query: 365 SREVRLTTPVSEEDIRSLRVGDVVILSGEMHTGRDALHKYLMDHEAPVDLNGAVIYHCGP 424 RE+ L P++EE IR LRVGDVV ++G M+TGRDA+HK+LMD++ PVDLNG +IYHCGP Sbjct: 313 QREIVLQAPITEEQIRELRVGDVVTINGMMYTGRDAIHKHLMDNDCPVDLNGQIIYHCGP 372 Query: 425 VMLKDE-EGWHVKAAGPTTSIREEPYQGDIIKKFGIRAVIGKGGMGPKTLKALQEHGGVY 483 V++KDE + W +KAAGPTTSIREEPYQGDI+KKFGIRAVIGKGGMG KTL AL+EHGGVY Sbjct: 373 VVVKDENDNWQIKAAGPTTSIREEPYQGDIMKKFGIRAVIGKGGMGAKTLAALEEHGGVY 432 Query: 484 LNAIGGAAQYYAECIKKVNGVDFMEFGIPEAMWHLQVDGFAAIVTMDAHGNSLHADVEKD 543 LNAIGGAAQYYAECIK+V VDF++FGIPEAMWHL++DGF A+VTMD+HGNSLHADV+K Sbjct: 433 LNAIGGAAQYYAECIKEVKDVDFLQFGIPEAMWHLRIDGFKAVVTMDSHGNSLHADVDKT 492 Query: 544 SAAKLAQFREPVFK 557 S KLA F+EPVFK Sbjct: 493 SLEKLASFKEPVFK 506 >ref|NP_654360.1| hypothetical protein predicted by GeneMark [Bacillus anthracis A2012] ref|NP_842966.1| fumarate hydratase, class I [Bacillus anthracis str. Ames] gb|AAP24452.1| fumarate hydratase, class I [Bacillus anthracis str. Ames] Length = 506 Score = 446 bits (1148), Expect = e-124, Method: Compositional matrix adjust. Identities = 216/299 (72%), Positives = 256/299 (85%) Query: 1 MQHFEDSVYDLIVETSTNLPGDVRRAVAKGRALEDRATRSGLALTTIAQNIGMAELQVSP 60 M+ ++S+Y LIVETSTNLP DVRRA+ + + E+ TRS +AL TI NI MA+ +SP Sbjct: 1 MEKLQESMYQLIVETSTNLPKDVRRAIQQAKERENAGTRSAMALGTITNNIKMADDNISP 60 Query: 61 ICQDTGMPTFIIHTPVGVNQIEMKKDIHNAIIRATINGKLRPNSVDSLTGENSGNNLGAG 120 ICQDTGMPTF I+TPVGVNQ+++K+ I++A+ RAT +GKLRPNSVDSL G+NSGNNLG G Sbjct: 61 ICQDTGMPTFKIYTPVGVNQLKLKEAIYHALERATKDGKLRPNSVDSLFGDNSGNNLGPG 120 Query: 121 TPVIHFEQWEETGVDVRLILKGGGCENKNIQYSLPAELEGLGKAGRDLDGIRKCILHSVY 180 TPVI FEQWE+ +D RLILKGGGCENKNIQYSLP ELEGLG+AGRDL+GIRKC+LH+VY Sbjct: 121 TPVIKFEQWEKDYIDARLILKGGGCENKNIQYSLPCELEGLGRAGRDLEGIRKCLLHAVY 180 Query: 181 QAQGQGCSAGFIGVGIGGDRTTGYELAKKQLFREVEDVNPNDDLAKLEEYIMENANKLGI 240 QAQGQGCSAG IGVGIGGDRT+GYELAK QLFR ++DVNP +L +LEEY++ENANKLGI Sbjct: 181 QAQGQGCSAGVIGVGIGGDRTSGYELAKNQLFRTLDDVNPIPELQQLEEYVLENANKLGI 240 Query: 241 GTMGFGGEVTLLGCKIGVMNRLPASFFVSVAYNCWAFRRQGILVDPSTGHIQEWLYESG 299 GTMGFGGE TLLGCKIGV NRLPASF+VSVAYNCWA+RR G+ + P TG I +WLY+ G Sbjct: 241 GTMGFGGETTLLGCKIGVYNRLPASFYVSVAYNCWAYRRLGVTIHPETGEIMDWLYQEG 299 Score = 318 bits (815), Expect = 2e-85, Method: Compositional matrix adjust. Identities = 146/194 (75%), Positives = 172/194 (88%), Gaps = 1/194 (0%) Query: 365 SREVRLTTPVSEEDIRSLRVGDVVILSGEMHTGRDALHKYLMDHEAPVDLNGAVIYHCGP 424 RE+ L P++EE IR LRVGDVV ++G M+TGRDA+HK+LMD++ PVDLNG VIYHCGP Sbjct: 313 QREIVLQAPITEEQIRKLRVGDVVTINGMMYTGRDAIHKHLMDNDCPVDLNGQVIYHCGP 372 Query: 425 VMLKDE-EGWHVKAAGPTTSIREEPYQGDIIKKFGIRAVIGKGGMGPKTLKALQEHGGVY 483 V++KDE + W +KAAGPTTSIREEPYQGDI+KKFGIRAVIGKGGMG KTL AL+EHGGVY Sbjct: 373 VVVKDENDNWQIKAAGPTTSIREEPYQGDIMKKFGIRAVIGKGGMGAKTLAALEEHGGVY 432 Query: 484 LNAIGGAAQYYAECIKKVNGVDFMEFGIPEAMWHLQVDGFAAIVTMDAHGNSLHADVEKD 543 LNAIGGAAQYYAECIK+V VDF++FGIPEAMWHL+++GF A+VTMD+HGNSLHADV+K Sbjct: 433 LNAIGGAAQYYAECIKEVKDVDFLQFGIPEAMWHLRIEGFKAVVTMDSHGNSLHADVDKT 492 Query: 544 SAAKLAQFREPVFK 557 S KLA F+EPVFK Sbjct: 493 SLEKLASFKEPVFK 506 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.138 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 784,137,004 Number of extensions: 33996909 Number of successful extensions: 77833 Number of sequences better than 1.0e-05: 213 Number of HSP's gapped: 77355 Number of HSP's successfully gapped: 256 Length of query: 557 Length of database: 757,237,578 Length adjustment: 131 Effective length of query: 426 Effective length of database: 401,559,609 Effective search space: 171064393434 Effective search space used: 171064393434 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)