BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0271.0012.p (114 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_578837.1| putative alpha-dextrin endo-1, 6-alpha-glucosid... 93 2e-18 sp|P38939|APU_THEET Amylopullulanase precursor (Alpha-amylase/pu... 77 2e-13 sp|P16950|APU_THETY Amylopullulanase precursor (Alpha-amylase/pu... 74 1e-12 gb|AAB00841.1| pullulanase [Thermoanaerobacterium thermosulfurig... 66 2e-10 sp|P38536|APU_THETU Amylopullulanase precursor (Alpha-amylase/pu... 66 2e-10 >ref|NP_578837.1| putative alpha-dextrin endo-1, 6-alpha-glucosidase [Pyrococcus furiosus DSM 3638] gb|AAL81232.1| putative alpha-dextrin endo-1, 6-alpha-glucosidase [Pyrococcus furiosus DSM 3638] Length = 404 Score = 92.8 bits (229), Expect = 2e-18, Method: Composition-based stats. Identities = 45/102 (44%), Positives = 61/102 (59%), Gaps = 5/102 (4%) Query: 14 SVTFRVSVPGTTPANGPLYLTGSFNSWNAADPAYQLTKGSDGVYSVTVSLPAGTAVQYKI 73 +VTF VSVP TP N +Y+ G FN+WN D Y+L K DG + +T++ P G +Q+K Sbjct: 47 TVTFIVSVPEYTPENDSIYIAGDFNNWNPKDERYKLVKLPDGRWKITLTFPYGKTIQFKF 106 Query: 74 TRGGWDTVETSSSGADIANR--TLTPAGGAQTVNLTVQRWKD 113 TRG W+TVE +G +I NR T T +G T V W+D Sbjct: 107 TRGSWETVEKGINGEEIPNRRFTFTKSG---TYEFKVHNWRD 145 >sp|P38939|APU_THEET Amylopullulanase precursor (Alpha-amylase/pullulanase) [Includes: Alpha-amylase (1,4-alpha-D-glucan glucanohydrolase); Pullulanase (1,4-alpha-D-glucan glucanohydrolase) (Alpha-dextrin endo-1,6-alpha-glucosidase)] pir||S28669 pullulanase (EC 3.2.1.41) - Thermoanaerobacter ethanolicus (strain 39E) gb|AAA23201.1| amylopullulanase Length = 1481 Score = 76.6 bits (187), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 44/105 (41%), Positives = 60/105 (57%), Gaps = 8/105 (7%) Query: 14 SVTFRVSVPGTTPANGPLYLTGSFNS--WNAADPAYQLTKGSDGVYSVTVSLPAGTAVQY 71 V F V+VP TP +G + G+F+ WN + A+Q+TK YS+T++L GT ++Y Sbjct: 1256 KVIFNVTVPDYTPDDGA-NIAGNFHDAFWNPS--AHQMTKTGPNTYSITLTLNEGTQLEY 1312 Query: 72 KITRGGWDTVETSSSGADIANRTLT---PAGGAQTVNLTVQRWKD 113 K RG WD VE G +IANR +T VN TVQRW+D Sbjct: 1313 KYARGSWDKVEKGEYGEEIANRKITVVNQGSNTMVVNDTVQRWRD 1357 >sp|P16950|APU_THETY Amylopullulanase precursor (Alpha-amylase/pullulanase) [Includes: Alpha-amylase (1,4-alpha-D-glucan glucanohydrolase); Pullulanase (1,4-alpha-D-glucan glucanohydrolase) (Alpha-dextrin endo-1,6-alpha-glucosidase)] pir||A44765 alpha-amylase (EC 3.2.1.1) / pullulanase (EC 3.2.1.41) precursor - Thermoanaerobacter thermohydrosulfuricus gb|AAA23205.1| alpha-amylase-pullulanase precursor (gtg start codon) Length = 1475 Score = 73.9 bits (180), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 43/105 (40%), Positives = 58/105 (55%), Gaps = 8/105 (7%) Query: 14 SVTFRVSVPGTTPANGPLYLTGSFNS--WNAADPAYQLTKGSDGVYSVTVSLPAGTAVQY 71 VTF V++P TP +G + + G+F WN A Q+TK YS+T++L GT ++Y Sbjct: 1261 KVTFNVTIPDYTPDDG-VNIAGNFPDAFWNPN--ANQMTKAGSNTYSITLTLNEGTQIEY 1317 Query: 72 KITRGGWDTVETSSSGADIANRTLT---PAGGAQTVNLTVQRWKD 113 K RG WD VE G +I NR +T VN TVQRW+D Sbjct: 1318 KYARGSWDKVEKGEYGNEIDNRKITVVNQGSNTMVVNDTVQRWRD 1362 >gb|AAB00841.1| pullulanase [Thermoanaerobacterium thermosulfurigenes] Length = 1861 Score = 66.2 bits (160), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 43/106 (40%), Positives = 58/106 (54%), Gaps = 12/106 (11%) Query: 15 VTFRVSVPGTTPANGPLYLTGSF--NSWNAADP-AYQLTKGSDGVYSVTVSLPAGTAVQY 71 V F V+VP TP + L G+F +W DP A Q+TK + YS+T++L GT ++Y Sbjct: 1254 VIFNVTVPDYTP--DAVNLAGTFPNATW---DPSAQQMTKIDNNTYSITLTLDEGTQIEY 1308 Query: 72 KITRGGWDTVETSSSGADIA-NRTLT---PAGGAQTVNLTVQRWKD 113 K RG WD VE G + A NR +T T+N TV RW+D Sbjct: 1309 KYARGSWDKVEKDEYGNEFASNRKVTIVNQGNNEMTINDTVYRWRD 1354 >sp|P38536|APU_THETU Amylopullulanase precursor (Alpha-amylase/pullulanase) (Pullulanase type II) [Includes: Alpha-amylase (1,4-alpha-D-glucan glucanohydrolase); Pullulanase (1,4-alpha-D-glucan glucanohydrolase) (Alpha-dextrin endo-1,6-alpha-glucosidase)] Length = 1861 Score = 66.2 bits (160), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 43/106 (40%), Positives = 58/106 (54%), Gaps = 12/106 (11%) Query: 15 VTFRVSVPGTTPANGPLYLTGSF--NSWNAADP-AYQLTKGSDGVYSVTVSLPAGTAVQY 71 V F V+VP TP + L G+F +W DP A Q+TK + YS+T++L GT ++Y Sbjct: 1254 VIFNVTVPDYTP--DAVNLAGTFPNATW---DPSAQQMTKIDNNTYSITLTLDEGTQIEY 1308 Query: 72 KITRGGWDTVETSSSGADIA-NRTLT---PAGGAQTVNLTVQRWKD 113 K RG WD VE G + A NR +T T+N TV RW+D Sbjct: 1309 KYARGSWDKVEKDEYGNEFASNRKVTIVNQGNNEMTINDTVYRWRD 1354 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.309 0.126 0.373 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 177,572,718 Number of extensions: 6704896 Number of successful extensions: 15434 Number of sequences better than 1.0e-05: 6 Number of HSP's gapped: 15448 Number of HSP's successfully gapped: 6 Length of query: 114 Length of database: 757,237,578 Length adjustment: 79 Effective length of query: 35 Effective length of database: 542,744,757 Effective search space: 18996066495 Effective search space used: 18996066495 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.7 bits) S2: 120 (50.8 bits)