BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0270.0001.p (237 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_657221.1| hypothetical protein predicted by GeneMark [Bac... 149 6e-35 dbj|BAC02729.1| hypothetical protein [Bacillus licheniformis] 141 1e-32 ref|NP_391611.1| similar to hypothetical proteins [Bacillus subt... 138 9e-32 ref|ZP_00123323.1| hypothetical protein [Haemophilus somnus 129PT] 128 1e-28 ref|ZP_00133299.1| COG0477: Permeases of the major facilitator s... 127 3e-28 >ref|NP_657221.1| hypothetical protein predicted by GeneMark [Bacillus anthracis A2012] ref|NP_845648.1| membrane protein, putative [Bacillus anthracis str. Ames] gb|AAP27134.1| membrane protein, putative [Bacillus anthracis str. Ames] Length = 240 Score = 149 bits (375), Expect = 6e-35, Method: Compositional matrix adjust. Identities = 87/226 (38%), Positives = 125/226 (55%) Query: 1 MKRFIPNQHGAWSMLVLPFLLGMAASQAKLLHIPLFLCWLLIYLFIFPLLQGVKTRRFDR 60 MK IP QHGAW+MLV+PFLL + + + HIPLF+ W IYL +P L +K +R Sbjct: 1 MKLVIPKQHGAWAMLVIPFLLSVILGKPTIYHIPLFIAWFFIYLATYPFLMYIKQKRKKE 60 Query: 61 YGPPLKMYGLLLLPFAAYLIVTEPKXXXXXXXXXXXXXVNLYYARTKNERALLNDIAGIL 120 Y +Y ++ F ++ E + VN+YYAR KNERAL NDI+ I+ Sbjct: 61 YLHAAIVYFIIAFVFGMISLLYEWRILLFVAVMIPFFIVNMYYARQKNERALRNDISAII 120 Query: 121 LFCLMVFPVFYIGGGTDWGIAGELFILALLYFVGTALYVKTIIREKNNPRYYYASVIYHG 180 +FC+ +Y A + +++ LYF+G+ YVKT+IREKNNP+Y + S YH Sbjct: 121 VFCIGGLVSYYFSMKLIDKTALFIALISFLYFLGSTFYVKTMIREKNNPKYRFISWGYHI 180 Query: 181 LFLLAGVVLFPSLTVPLIILLARAASLPKTKITAKRTGILEIGFSV 226 L ++ + P ++ I + RA L KI+ + GILEI SV Sbjct: 181 LLMIIVFAINPWCSLIFIPSVIRAIILYGKKISIIKVGILEIVNSV 226 >dbj|BAC02729.1| hypothetical protein [Bacillus licheniformis] Length = 240 Score = 141 bits (356), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 79/179 (44%), Positives = 104/179 (58%) Query: 1 MKRFIPNQHGAWSMLVLPFLLGMAASQAKLLHIPLFLCWLLIYLFIFPLLQGVKTRRFDR 60 MK FIP QHGAW+MLV+PF+LGM A A + HIPLF WL +YL FP VK + Sbjct: 1 MKVFIPKQHGAWAMLVIPFVLGMFAGGADVKHIPLFCGWLFLYLAAFPATMMVKKKNIVY 60 Query: 61 YGPPLKMYGLLLLPFAAYLIVTEPKXXXXXXXXXXXXXVNLYYARTKNERALLNDIAGIL 120 Y + +YG+ + F + EP+ +NLYYA+ N+RALLNDIA IL Sbjct: 61 YQKWVLIYGIPAIVFLLISVWLEPRLVFLGLPLFPLFLINLYYAKRNNDRALLNDIAAIL 120 Query: 121 LFCLMVFPVFYIGGGTDWGIAGELFILALLYFVGTALYVKTIIREKNNPRYYYASVIYH 179 +F F++G G G A +FI + L+F G+A YVK++IREK N R+ S YH Sbjct: 121 VFSAGGLASFWMGAGKLDGEALFVFIQSALFFTGSAFYVKSMIREKKNNRFKLYSWGYH 179 >ref|NP_391611.1| similar to hypothetical proteins [Bacillus subtilis] sp|P46909|YWIC_BACSU Hypothetical protein ywiC pir||S60083 conserved hypothetical protein ywiC - Bacillus subtilis emb|CAA90043.1| unknown [Bacillus subtilis] emb|CAB15758.1| ywiC [Bacillus subtilis subsp. subtilis str. 168] Length = 239 Score = 138 bits (348), Expect = 9e-32, Method: Compositional matrix adjust. Identities = 72/179 (40%), Positives = 105/179 (58%) Query: 1 MKRFIPNQHGAWSMLVLPFLLGMAASQAKLLHIPLFLCWLLIYLFIFPLLQGVKTRRFDR 60 MK IP QHGAW+ML++PFLLGM + HIPLFL WL +YL ++P+ +K ++ Sbjct: 1 MKALIPKQHGAWAMLLIPFLLGMVKGGPVIWHIPLFLGWLFLYLAVYPVTLALKKKQSKP 60 Query: 61 YGPPLKMYGLLLLPFAAYLIVTEPKXXXXXXXXXXXXXVNLYYARTKNERALLNDIAGIL 120 Y + YG F + +P +++Y+AR KNERALLND+AG+L Sbjct: 61 YQKWMCYYGFPTCCFLMISVFHKPPLIWVGVSLLPLFLIHMYFARRKNERALLNDVAGVL 120 Query: 121 LFCLMVFPVFYIGGGTDWGIAGELFILALLYFVGTALYVKTIIREKNNPRYYYASVIYH 179 FC F ++G GT G A +F+ + L+F+G++ YVK++IRE+ N + Y S YH Sbjct: 121 FFCSGGFASCWLGMGTLDGWAWFIFLQSALFFIGSSFYVKSVIRERKNRAFAYWSWGYH 179 >ref|ZP_00123323.1| hypothetical protein [Haemophilus somnus 129PT] Length = 240 Score = 128 bits (321), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 85/242 (35%), Positives = 129/242 (53%), Gaps = 17/242 (7%) Query: 1 MKRFIPNQHGAWSMLVLPFLLGMAASQAKLLHIPLFLCWLLIYLFIFPLLQGVKTRR--F 58 MK I NQHGA M ++PFL GM ++ H LFL W +YL +P K ++ F Sbjct: 1 MKMLISNQHGATLMALMPFLYGMLLAKPTWQHSFLFLAWFSLYLMSYPFFHLFKGKKSNF 60 Query: 59 DRYGPPLKMYGLLLLPFAAYLIVTEPKXXXXXXXXXXXXXVNLYYARTKNERALLNDIAG 118 +Y +YG L FA + +N+Y+ + KNERAL+ND+AG Sbjct: 61 IKYQKYTLIYGTTSLIFALPVFYYNWHILYFLIAMLPFGFINIYFVKQKNERALINDLAG 120 Query: 119 ILLFCLMVFPVFYIGGGTDWGIAGELFILAL---LYFVGTALYVKTIIREKNNPRYYYAS 175 I++F + +Y T +++++AL LYF+GT LYVK+ +RE+ NP+Y AS Sbjct: 121 IIIFAIAGMASYYFSTFT---FTQQIWLVALLPSLYFIGTTLYVKSCLRERKNPKYLLAS 177 Query: 176 VIYHGLFLLAGVVLFPS-----LTVPLIILLARAASLPKTKITAKRTGILEIGFSVMLYI 230 ++H A VVLF + L +I RA LPK KI+AK+ G++EI +++ +I Sbjct: 178 YLWH----FACVVLFVAIQQFYLAFAFVIPAIRAYFLPKMKISAKQLGLIEIAVALVFFI 233 Query: 231 SV 232 + Sbjct: 234 QL 235 >ref|ZP_00133299.1| COG0477: Permeases of the major facilitator superfamily [Haemophilus somnus 2336] Length = 240 Score = 127 bits (318), Expect = 3e-28, Method: Compositional matrix adjust. Identities = 85/242 (35%), Positives = 129/242 (53%), Gaps = 17/242 (7%) Query: 1 MKRFIPNQHGAWSMLVLPFLLGMAASQAKLLHIPLFLCWLLIYLFIFPLLQGVKTRR--F 58 MK I NQHGA M ++PFL GM ++ H LFL W +YL +P K ++ F Sbjct: 1 MKLLISNQHGATLMALMPFLYGMLLAKPTWQHSFLFLAWFSLYLMSYPFFHLFKGKKSNF 60 Query: 59 DRYGPPLKMYGLLLLPFAAYLIVTEPKXXXXXXXXXXXXXVNLYYARTKNERALLNDIAG 118 +Y +YG L FA + +N+Y+ + KNERAL+ND AG Sbjct: 61 IKYQKYTLIYGTTSLIFALPVFYYNWHILYFLIAMLPFGFINIYFVKQKNERALINDSAG 120 Query: 119 ILLFCLMVFPVFYIGGGTDWGIAGELFILAL---LYFVGTALYVKTIIREKNNPRYYYAS 175 I++F + +Y T +++++AL LYF+GT LYVK+ +RE+ NP+Y AS Sbjct: 121 IIIFAIAGMASYYFSTFT---FTQQIWLVALLPSLYFIGTTLYVKSCLRERKNPKYLLAS 177 Query: 176 VIYHGLFLLAGVVLFPS-----LTVPLIILLARAASLPKTKITAKRTGILEIGFSVMLYI 230 ++H +A VVLF + L +I RA LPK KI+AK+ G++EI +++ +I Sbjct: 178 YLWH----VACVVLFVAIQQFYLAFAFVIPAIRAYFLPKMKISAKQLGLIEIAVALVFFI 233 Query: 231 SV 232 + Sbjct: 234 QL 235 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.333 0.149 0.467 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 324,621,921 Number of extensions: 12317628 Number of successful extensions: 38115 Number of sequences better than 1.0e-05: 14 Number of HSP's gapped: 38091 Number of HSP's successfully gapped: 14 Length of query: 237 Length of database: 757,237,578 Length adjustment: 122 Effective length of query: 115 Effective length of database: 425,995,500 Effective search space: 48989482500 Effective search space used: 48989482500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (22.0 bits) S2: 124 (52.4 bits)