BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0264.0063.p (216 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_391642.1| alternate gene name: ipa-92r~similar to hypothe... 150 1e-35 ref|NP_653850.1| hypothetical protein predicted by GeneMark [Bac... 138 1e-31 ref|NP_658429.1| hypothetical protein predicted by GeneMark [Bac... 137 2e-31 ref|NP_980776.1| prespore-specific transcriptional regulator rsf... 135 5e-31 ref|NP_242122.1| BH1256~unknown conserved protein in B. subtilis... 135 9e-31 >ref|NP_391642.1| alternate gene name: ipa-92r~similar to hypothetical proteins from B. subtilis [Bacillus subtilis] sp|P39650|RSFA_BACSU Prespore specific transcriptional regulator rsfA pir||S39747 ywfN protein - Bacillus subtilis emb|CAA51648.1| ipa-92r [Bacillus subtilis] emb|CAB15789.1| probable transcriptional regulatory protein [Bacillus subtilis subsp. subtilis str. 168] Length = 258 Score = 150 bits (380), Expect = 1e-35, Method: Compositional matrix adjust. Identities = 90/261 (34%), Positives = 127/261 (48%), Gaps = 51/261 (19%) Query: 1 MTAVRQDAWSAEDDLILAEITLRHIREGSTQLAAFEEVGEKIGRTSAACGFRWNSCVRKS 60 MT RQDAWS E+DL+LAE LRH+REGSTQL AFEEVG+K+ RTSAACGFRWN+ VR Sbjct: 1 MTKQRQDAWSEENDLLLAETVLRHVREGSTQLNAFEEVGDKLNRTSAACGFRWNAVVRHQ 60 Query: 61 YEDAIGIAKSQRQKR----------------------------------------SYLKK 80 YE A+ +AK QR++R S + Sbjct: 61 YEKALQLAKKQRKQRMRALGNGQPAKKRLLYQPPAVDPEIIQETAAEEPVKTETPSVENE 120 Query: 81 QPSARGAQVAGLI------LGDIXXXXXXXXXXXXNSLSIDAVIRFLRQWKGSFQEAGRQ 134 QP G + + L + ++++ VIRFL+ ++G+ +++ Sbjct: 121 QPLMSGEHMPFVDESFKEELASLSHLLSPQTPAAAPEMTMNDVIRFLQNYEGNHEQSS-A 179 Query: 135 LKMXXXXXXXXXXXXXXXXXXXXXXSKEVNLAQSDYRVVNDDYKALIQIMDRARRLAFLN 194 LKM EV + D + + +DY+ L++IM+RAR+L Sbjct: 180 LKMENERLKKENQELQNKTEQLEA---EVQKLEKDQKTIQEDYETLVKIMNRARKLVLF- 235 Query: 195 EEEEEMKTRFKMDANGNLERI 215 EE+E FKMD NGNLE++ Sbjct: 236 EEDEHASPSFKMDRNGNLEKL 256 >ref|NP_653850.1| hypothetical protein predicted by GeneMark [Bacillus anthracis A2012] ref|NP_847783.1| conserved hypothetical protein [Bacillus anthracis str. Ames] gb|AAP29269.1| conserved hypothetical protein [Bacillus anthracis str. Ames] Length = 230 Score = 138 bits (347), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 88/237 (37%), Positives = 117/237 (49%), Gaps = 36/237 (15%) Query: 4 VRQDAWSAEDDLILAEITLRHIREGSTQLAAFEEVGEKIGRTSAACGFRWNSCVRKSYED 63 VRQDAW+ EDDL+LAE LRH+REGSTQL AFEEVG+K+ RTSAACGFRWN+ VR SYE Sbjct: 3 VRQDAWTDEDDLLLAETVLRHVREGSTQLNAFEEVGDKLNRTSAACGFRWNAVVRYSYEQ 62 Query: 64 AIGIAKSQR----------QKRSYLKKQPSARGA---------QVAGLILGDIXXXXXXX 104 A+ +AK R Q + L P A GA + + + Sbjct: 63 ALQLAKKHRKDKMRAASGEQAKKRLLYTPPASGAITDYDELVQEETTVQYKEAIPYQEST 122 Query: 105 XXXXXNSLSIDAVIRFLRQWKGS------FQEAGRQLKMXXXXXXXXXXXXXXXXXXXXX 158 S+++ VI FL+ S + +LK Sbjct: 123 VPAAKGSMTMQDVIYFLQTVGSSNIKVTALENENTRLKQEMKAQILRNEELEKKLEKLEK 182 Query: 159 XSKEVNLAQSDYRVVNDDYKALIQIMDRARRLAFLNEEEEEMKTRFKMDANGNLERI 215 S V +DY+ L+ IM+RAR+LA +++EE +T F+MD NGNLE+I Sbjct: 183 QS----------HTVQEDYETLMNIMNRARKLAVMDDEERS-QTSFRMDRNGNLEKI 228 >ref|NP_658429.1| hypothetical protein predicted by GeneMark [Bacillus anthracis A2012] ref|NP_846847.1| prespore-specific transcriptional regulator rsfA, putative [Bacillus anthracis str. Ames] gb|AAP28333.1| prespore-specific transcriptional regulator rsfA, putative [Bacillus anthracis str. Ames] Length = 213 Score = 137 bits (344), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 81/216 (37%), Positives = 114/216 (52%), Gaps = 14/216 (6%) Query: 4 VRQDAWSAEDDLILAEITLRHIREGSTQLAAFEEVGEKIGRTSAACGFRWNSCVRKSYED 63 RQDAW+ EDDL+LAE LRHIR GSTQ+ AF+EVG+ + RTSAACGFRWN+ VR +YED Sbjct: 3 TRQDAWTKEDDLLLAETVLRHIRSGSTQIKAFDEVGDALNRTSAACGFRWNAEVRPNYED 62 Query: 64 AIGIAKSQRQ--KRSYLK--KQPSARGAQVAGLILGDIXXXXXXXXXXXXNSLSIDAVIR 119 A+ +AK QR+ KRS K K+ + Q+ I L++ VI Sbjct: 63 AVQLAKKQRKELKRSEAKIEKEHFTKTKQLV------IDAEFSEDIAPSKQELTMQNVIS 116 Query: 120 FLRQWKGSFQEAGRQLKMXXXXXXXXXXXXXXXXXXXXXXSKEVNLAQSDYRVVNDDYKA 179 FL+ + + L ++ + + + +DY Sbjct: 117 FLQ----NLEHTNPSLAKLQTENDVLQAQLTSLQKTNDELETKLAVLTKKQQAIEEDYAM 172 Query: 180 LIQIMDRARRLAFLNEEEEEMKTRFKMDANGNLERI 215 L++IMDRAR+L + E+EE++ FK D NGNL+ I Sbjct: 173 LVRIMDRARKLVSVEEQEEQIAPIFKTDQNGNLDII 208 >ref|NP_980776.1| prespore-specific transcriptional regulator rsfA, putative [Bacillus cereus ATCC 10987] gb|AAS43384.1| prespore-specific transcriptional regulator rsfA, putative [Bacillus cereus ATCC 10987] Length = 218 Score = 135 bits (341), Expect = 5e-31, Method: Compositional matrix adjust. Identities = 83/219 (37%), Positives = 116/219 (52%), Gaps = 14/219 (6%) Query: 1 MTAVRQDAWSAEDDLILAEITLRHIREGSTQLAAFEEVGEKIGRTSAACGFRWNSCVRKS 60 + RQDAW+ EDDL+LAE LRHIR GSTQ+ AF+EVG+ + RTSAACGFRWN+ VR + Sbjct: 5 LMKTRQDAWTKEDDLLLAETVLRHIRSGSTQIKAFDEVGDALNRTSAACGFRWNAEVRPN 64 Query: 61 YEDAIGIAKSQRQ--KRSYLK--KQPSARGAQVAGLILGDIXXXXXXXXXXXXNSLSIDA 116 YEDA+ +AK QR+ KRS K K + Q+ I L++ Sbjct: 65 YEDAVQLAKKQRKELKRSEAKIEKNQFTKTKQLV------IDAEFSEDIVPSKQELTMQN 118 Query: 117 VIRFLRQWKGSFQEAGRQLKMXXXXXXXXXXXXXXXXXXXXXXSKEVNLAQSDYRVVNDD 176 VI FL+ + + K+ +K L + + + +D Sbjct: 119 VISFLQNLEHNNPSIA---KLQTENEVLQTQLTSLQKTNDELETKLAVLTKKQ-QAIEED 174 Query: 177 YKALIQIMDRARRLAFLNEEEEEMKTRFKMDANGNLERI 215 Y L++IMDRAR+L + E+EE++ FK D NGNL+ I Sbjct: 175 YAMLVRIMDRARKLVSVEEQEEQIAPIFKTDQNGNLDII 213 >ref|NP_242122.1| BH1256~unknown conserved protein in B. subtilis [Bacillus halodurans] pir||H83806 hypothetical protein BH1256 [imported] - Bacillus halodurans (strain C-125) dbj|BAB04975.1| BH1256~unknown conserved protein in B. subtilis [Bacillus halodurans] Length = 203 Score = 135 bits (339), Expect = 9e-31, Method: Compositional matrix adjust. Identities = 84/217 (38%), Positives = 120/217 (55%), Gaps = 23/217 (10%) Query: 4 VRQDAWSAEDDLILAEITLRHIREGSTQLAAFEEVGEKIGRTSAACGFRWNSCVRKSYED 63 VRQDAWS EDD+ LAE L+HI EGSTQL AF+EVG+ + RTSAACGFRWN+ VR+ Y Sbjct: 3 VRQDAWSHEDDVFLAETVLKHIEEGSTQLRAFDEVGDVLNRTSAACGFRWNAVVRQRYVK 62 Query: 64 AI----GIAKSQRQKRSY-LKKQPSARGAQVAGLILGDIXXXXXXXXXXXXNSLSIDAVI 118 I K +++ SY + P+A +Q ++ G+ SL++ VI Sbjct: 63 QIAEAKKERKRRKRAASYAYQLYPTA--SQQPVVLTGE------------ATSLTLPMVI 108 Query: 119 RFLRQWKGSFQEAGRQLKMXXXXXXXXXXXXXXXXXXXXXXSKEVNLAQSDYRVVNDDYK 178 FL+Q + Q A Q + KE+ + ++ ++ +DY+ Sbjct: 109 EFLQQL-ATHQVADGQTR---KEQEELMKQNEQLQERNKELEKELQKIKQEHSIIEEDYQ 164 Query: 179 ALIQIMDRARRLAFLNEEEEEMKTRFKMDANGNLERI 215 ++IQIM+RARR+A L ++E FKMD NGNLE+I Sbjct: 165 SMIQIMNRARRMAILQDDEPASTQAFKMDKNGNLEKI 201 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.131 0.368 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 233,141,295 Number of extensions: 7001810 Number of successful extensions: 16353 Number of sequences better than 1.0e-05: 14 Number of HSP's gapped: 16350 Number of HSP's successfully gapped: 16 Length of query: 216 Length of database: 757,237,578 Length adjustment: 121 Effective length of query: 95 Effective length of database: 428,710,599 Effective search space: 40727506905 Effective search space used: 40727506905 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 123 (52.0 bits)