BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0264.0055.p (423 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00098522.1| COG0499: S-adenosylhomocysteine hydrolase [De... 560 e-158 ref|ZP_00061499.1| COG0499: S-adenosylhomocysteine hydrolase [Cl... 553 e-156 ref|NP_127177.1| adenosylhomocysteinase [Pyrococcus abyssi] >gi|... 529 e-149 ref|NP_578072.1| s-adenosylhomocysteinase [Pyrococcus furiosus D... 528 e-149 ref|NP_142509.1| S-adenosyl-L-homocysteine hydrolase [Pyrococcus... 519 e-146 >ref|ZP_00098522.1| COG0499: S-adenosylhomocysteine hydrolase [Desulfitobacterium hafniense] Length = 420 Score = 560 bits (1443), Expect = e-158, Method: Compositional matrix adjust. Identities = 276/420 (65%), Positives = 329/420 (78%), Gaps = 2/420 (0%) Query: 1 MSSLSKEN--SIVADMSLAPEGHLKIDWVRQHMPVLNRIREQFEAEQPFKGLKVSITLHL 58 M+ LS++N S + D+ LAP+G LKIDWV+ HMPVLN +RE+FE QPF+G KV+I LHL Sbjct: 1 MTWLSQDNMESTIRDIGLAPQGQLKIDWVQAHMPVLNTLREEFEKTQPFQGKKVTICLHL 60 Query: 59 EAKTAYLAKVVQAGGAEVTITGSNPLSTQDDVCAALVEDGITVFAKYNPSPEEFKALNIK 118 EAKTAYLAKVVQAGGAEVT+ SNPLSTQDDV AALV G+ A Y + EE+ K Sbjct: 61 EAKTAYLAKVVQAGGAEVTVAASNPLSTQDDVVAALVAGGVHAHAWYGATDEEYHQHLHK 120 Query: 119 ALESKPDLIIDDGGDFATLLHSERPDLMENIRGGAEETTTGIIRLKALQKQGILKFPMVA 178 AL+ +PD IIDDGGD + LH ER +L+ + GGAEETTTGI+RL+A++K G L FPM+A Sbjct: 121 ALDFEPDYIIDDGGDLVSTLHKERRELLPKVLGGAEETTTGILRLRAMEKNGELAFPMMA 180 Query: 179 VNDAYCKYLFDNRYGTGQSAWDGIVRTTNLIVAGKTVVVVGYGWCGKGVAMRAKGLGANV 238 VNDA KYLFDNRYGTGQS WDGI+RTTNL+VAGKT+ VVGYGWCGKGVA+RAKGLGA V Sbjct: 181 VNDAEMKYLFDNRYGTGQSVWDGIMRTTNLVVAGKTICVVGYGWCGKGVALRAKGLGARV 240 Query: 239 IVTEVDAIKAVEAHMDGFHVMPMLEAAKRGDFFVTVTGNRYVIRGEHYDVMKDGAIMCNA 298 I+ EV+ IKA EA MDGF VMPML AA GDFFVTVTGN+ VI GEH+ VMKDGAI+ NA Sbjct: 241 IICEVNPIKANEAWMDGFEVMPMLAAAPLGDFFVTVTGNKNVISGEHFQVMKDGAILANA 300 Query: 299 GHFDVEVNKPELSDRSVSQRTVRKNIEEYQLKDGRKLYLLAEGRLVNLGAADGHPAEIMD 358 GHFDVEVNK +L+ + S VR+NIEEY L+DGRK+YLLAEGRLVNL A DGHPAE+MD Sbjct: 301 GHFDVEVNKVQLAAMAQSCHEVRRNIEEYLLQDGRKIYLLAEGRLVNLAAGDGHPAEVMD 360 Query: 359 TTFALQALSLKYVNDNYKTIGVKVENVPYDLDEQVARFKLESLGISIDSLTPAQVEYLDS 418 TFALQAL+L Y+ + KV +VP ++D +VA KL++LG+SID L+ Q YL S Sbjct: 361 MTFALQALALHYLATAKTPLEPKVYSVPTEMDMRVAELKLKTLGLSIDQLSEEQKSYLAS 420 >ref|ZP_00061499.1| COG0499: S-adenosylhomocysteine hydrolase [Clostridium thermocellum ATCC 27405] Length = 414 Score = 553 bits (1424), Expect = e-156, Method: Compositional matrix adjust. Identities = 263/412 (63%), Positives = 330/412 (80%), Gaps = 2/412 (0%) Query: 9 SIVADMSLAPEGHLKIDWVRQHMPVLNRIREQFEAEQPFKGLKVSITLHLEAKTAYLAKV 68 S++ D+SLA G KI+WVR++MP+L + E+F +PF G+++++++HLEAKTAYLAK+ Sbjct: 3 SVIRDISLAKSGRQKINWVRKNMPLLRSLEEEFAKTKPFDGIRITVSVHLEAKTAYLAKL 62 Query: 69 VQAGGAEVTITGSNPLSTQDDVCAALVEDGITVFAKYNPSPEEFKA-LNIKALESKPDLI 127 GG +V++TGSNPLSTQDDV AAL EDG+ V+A YN + +E+K LN+ AL+ +P+++ Sbjct: 63 FAIGGGQVSVTGSNPLSTQDDVAAALAEDGLNVYAWYNATEKEYKEHLNL-ALDGRPNIV 121 Query: 128 IDDGGDFATLLHSERPDLMENIRGGAEETTTGIIRLKALQKQGILKFPMVAVNDAYCKYL 187 IDDGGD LLH+ER +L+ ++ GG EETTTG+IRLKA++K+G LKFPMVAVN+AYCKYL Sbjct: 122 IDDGGDLVHLLHTERTELLSDVMGGCEETTTGVIRLKAMEKEGALKFPMVAVNNAYCKYL 181 Query: 188 FDNRYGTGQSAWDGIVRTTNLIVAGKTVVVVGYGWCGKGVAMRAKGLGANVIVTEVDAIK 247 FDNRYGTGQS WDGI RTTNLIVAGK VVVVGYGWCGKG+AMRAKGLGA+VIV EVD IK Sbjct: 182 FDNRYGTGQSVWDGINRTTNLIVAGKNVVVVGYGWCGKGIAMRAKGLGASVIVCEVDPIK 241 Query: 248 AVEAHMDGFHVMPMLEAAKRGDFFVTVTGNRYVIRGEHYDVMKDGAIMCNAGHFDVEVNK 307 A EA MDG+ VMPM+EAAK GD F+T TG VI EH+ VMKDGAI+CNAGHFDVEV+ Sbjct: 242 AAEAVMDGYKVMPMMEAAKIGDLFITATGCSRVIHREHFKVMKDGAILCNAGHFDVEVSV 301 Query: 308 PELSDRSVSQRTVRKNIEEYQLKDGRKLYLLAEGRLVNLGAADGHPAEIMDTTFALQALS 367 +L + +V + RKNI Y ++DGR + LLAEGRLVNL A DGHPAEIMD +FALQALS Sbjct: 302 KDLEEMAVRKEEQRKNIMGYMMEDGRWINLLAEGRLVNLAAGDGHPAEIMDMSFALQALS 361 Query: 368 LKYVNDNYKTIGVKVENVPYDLDEQVARFKLESLGISIDSLTPAQVEYLDSW 419 KYV +N+ +G KV +VP ++D +VA KLES GI+ID LT Q +YLDSW Sbjct: 362 AKYVLENHSRLGKKVIDVPEEIDRRVALMKLESWGITIDELTEEQKKYLDSW 413 >ref|NP_127177.1| adenosylhomocysteinase [Pyrococcus abyssi] sp|Q9UYK5|SAHH_PYRAB Adenosylhomocysteinase (S-adenosyl-L-homocysteine hydrolase) (AdoHcyase) pir||B75064 adenosylhomocysteinase (ahcy) PAB1372 - Pyrococcus abyssi (strain Orsay) emb|CAB50407.1| ahcY adenosylhomocysteinase (EC 3.3.1.1) [Pyrococcus abyssi] Length = 422 Score = 529 bits (1363), Expect = e-149, Method: Compositional matrix adjust. Identities = 248/410 (60%), Positives = 323/410 (78%) Query: 11 VADMSLAPEGHLKIDWVRQHMPVLNRIREQFEAEQPFKGLKVSITLHLEAKTAYLAKVVQ 70 V D+SLAPEG KIDWV + MPVL IR +FE ++PFKG++++ TLHLE KTA+L ++ Sbjct: 10 VKDISLAPEGMKKIDWVSRFMPVLQHIRREFEEKKPFKGVRIAATLHLEMKTAFLLLTLK 69 Query: 71 AGGAEVTITGSNPLSTQDDVCAALVEDGITVFAKYNPSPEEFKALNIKALESKPDLIIDD 130 AGGA+V+ SNPLSTQDDV AAL ++G+ V+A S EE+ KAL+ +P++IIDD Sbjct: 70 AGGAKVSAAASNPLSTQDDVVAALAKEGVKVYAIRGESKEEYYEFMNKALDIRPNIIIDD 129 Query: 131 GGDFATLLHSERPDLMENIRGGAEETTTGIIRLKALQKQGILKFPMVAVNDAYCKYLFDN 190 G D +L+H+ER +L++ I G +EETTTG+IRL+A+++ +L+FP++AVND+Y KYLFDN Sbjct: 130 GADMVSLVHTERKELLDEIWGASEETTTGVIRLRAMERDKVLRFPVIAVNDSYMKYLFDN 189 Query: 191 RYGTGQSAWDGIVRTTNLIVAGKTVVVVGYGWCGKGVAMRAKGLGANVIVTEVDAIKAVE 250 RYGTGQS WDGI+R TNL+VAGK VVVVGYGWCG+G+AMRA+GLGA VIV EVD IKA+E Sbjct: 190 RYGTGQSTWDGIMRATNLLVAGKNVVVVGYGWCGRGIAMRARGLGATVIVVEVDPIKALE 249 Query: 251 AHMDGFHVMPMLEAAKRGDFFVTVTGNRYVIRGEHYDVMKDGAIMCNAGHFDVEVNKPEL 310 A MDGF VM M EAAK GD FVT TGN IR EH+++MKDGAIM NAGHFDVE+ KP+L Sbjct: 250 ARMDGFLVMSMKEAAKIGDIFVTATGNIKCIRREHFELMKDGAIMANAGHFDVEIWKPDL 309 Query: 311 SDRSVSQRTVRKNIEEYQLKDGRKLYLLAEGRLVNLGAADGHPAEIMDTTFALQALSLKY 370 + +V R N+ EY+LKDGR+LYLLA+GRLVNL AADGHPAEIMD +FALQA + +Y Sbjct: 310 EELAVEISNPRPNVTEYKLKDGRRLYLLADGRLVNLVAADGHPAEIMDMSFALQAKAAEY 369 Query: 371 VNDNYKTIGVKVENVPYDLDEQVARFKLESLGISIDSLTPAQVEYLDSWN 420 + +N++ + +V +P ++DE VAR KL+S+GI+I+ LT Q +YL+SW Sbjct: 370 IKENHERLEPRVYVLPREIDEMVARIKLQSMGINIEELTEEQKKYLESWQ 419 >ref|NP_578072.1| s-adenosylhomocysteinase [Pyrococcus furiosus DSM 3638] sp|P50251|SAHH_PYRFU Adenosylhomocysteinase (S-adenosyl-L-homocysteine hydrolase) (AdoHcyase) gb|AAL80467.1| s-adenosylhomocysteinase [Pyrococcus furiosus DSM 3638] Length = 421 Score = 528 bits (1361), Expect = e-149, Method: Compositional matrix adjust. Identities = 252/409 (61%), Positives = 320/409 (78%) Query: 11 VADMSLAPEGHLKIDWVRQHMPVLNRIREQFEAEQPFKGLKVSITLHLEAKTAYLAKVVQ 70 V D+SLA EG KIDWV + MPVL I+ +FE ++PFKG++++ TLHLE KTA+L ++ Sbjct: 9 VKDLSLAEEGWKKIDWVSRFMPVLQYIKREFEEKKPFKGVRIAATLHLEMKTAFLLLTLK 68 Query: 71 AGGAEVTITGSNPLSTQDDVCAALVEDGITVFAKYNPSPEEFKALNIKALESKPDLIIDD 130 AGGAEV+ SNPLSTQDDV AAL + G+ V+A S E++ KAL+ +P++IIDD Sbjct: 69 AGGAEVSAAASNPLSTQDDVVAALAKAGVKVYAIRGESREQYYEFMHKALDIRPNIIIDD 128 Query: 131 GGDFATLLHSERPDLMENIRGGAEETTTGIIRLKALQKQGILKFPMVAVNDAYCKYLFDN 190 G D +L+H ER ++++ I GG+EETTTG+IRL+A++K GILKFP++AVND+Y KYLFDN Sbjct: 129 GADMISLVHKERQEMLDEIWGGSEETTTGVIRLRAMEKAGILKFPVIAVNDSYMKYLFDN 188 Query: 191 RYGTGQSAWDGIVRTTNLIVAGKTVVVVGYGWCGKGVAMRAKGLGANVIVTEVDAIKAVE 250 RYGTGQS WDGI+R TNL++AGK VVVVGYGWCG+G+AMRA+GLGA VIV EVD IKA+E Sbjct: 189 RYGTGQSTWDGIMRATNLLIAGKNVVVVGYGWCGRGIAMRARGLGATVIVVEVDPIKALE 248 Query: 251 AHMDGFHVMPMLEAAKRGDFFVTVTGNRYVIRGEHYDVMKDGAIMCNAGHFDVEVNKPEL 310 A MDGF VM M EAAK GD FVT TGN IR EH+++MKDGAIM NAGHFDVE+ KP+L Sbjct: 249 ARMDGFLVMDMKEAAKIGDIFVTATGNIKCIRREHFELMKDGAIMANAGHFDVEIWKPDL 308 Query: 311 SDRSVSQRTVRKNIEEYQLKDGRKLYLLAEGRLVNLGAADGHPAEIMDTTFALQALSLKY 370 +V R N+ EY+LKDGR+LYLLA+GRLVNL AADGHPAEIMD +FALQA + +Y Sbjct: 309 EKLAVEINNPRPNVTEYKLKDGRRLYLLADGRLVNLVAADGHPAEIMDMSFALQAKAAEY 368 Query: 371 VNDNYKTIGVKVENVPYDLDEQVARFKLESLGISIDSLTPAQVEYLDSW 419 + DN++ + KV +P ++DE VAR KLES+GI I+ LT Q +YL+SW Sbjct: 369 IKDNHERLEPKVYILPREIDEMVARIKLESMGIKIEELTEEQKKYLESW 417 >ref|NP_142509.1| S-adenosyl-L-homocysteine hydrolase [Pyrococcus horikoshii] pir||H71167 probable S-adenosyl-L-homocysteine hydrolase - Pyrococcus horikoshii dbj|BAA29629.1| 425aa long hypothetical S-adenosyl-L-homocysteine hydrolase [Pyrococcus horikoshii] Length = 425 Score = 519 bits (1337), Expect = e-146, Method: Compositional matrix adjust. Identities = 245/409 (59%), Positives = 318/409 (77%) Query: 11 VADMSLAPEGHLKIDWVRQHMPVLNRIREQFEAEQPFKGLKVSITLHLEAKTAYLAKVVQ 70 V D+SLA EG KIDWV + MPVL IR +FE ++PFKG++++ TLHLE KTA+L ++ Sbjct: 13 VKDLSLADEGWKKIDWVSRFMPVLQHIRREFEEKKPFKGVRIAATLHLEMKTAFLLLTLK 72 Query: 71 AGGAEVTITGSNPLSTQDDVCAALVEDGITVFAKYNPSPEEFKALNIKALESKPDLIIDD 130 AGGAEV+ SNPLSTQDDV AAL + G+ V+A S E++ KAL+ +P++IIDD Sbjct: 73 AGGAEVSAAASNPLSTQDDVVAALAKAGVKVYAIRGESKEQYYEFMHKALDIRPNIIIDD 132 Query: 131 GGDFATLLHSERPDLMENIRGGAEETTTGIIRLKALQKQGILKFPMVAVNDAYCKYLFDN 190 G D +L+H ER +L++ I GG+EETTTG+IRL+A+++ G+L+F ++AVND+Y KYLFDN Sbjct: 133 GADMISLVHKERQELLDEIWGGSEETTTGVIRLRAMERDGVLRFSVIAVNDSYMKYLFDN 192 Query: 191 RYGTGQSAWDGIVRTTNLIVAGKTVVVVGYGWCGKGVAMRAKGLGANVIVTEVDAIKAVE 250 RYGTGQS WDG++R TNL++AGK VVVVGYGWCG+G+AMRA+GLGA VIV EVD IKA+E Sbjct: 193 RYGTGQSTWDGVMRATNLLIAGKNVVVVGYGWCGRGIAMRARGLGATVIVVEVDPIKALE 252 Query: 251 AHMDGFHVMPMLEAAKRGDFFVTVTGNRYVIRGEHYDVMKDGAIMCNAGHFDVEVNKPEL 310 A MDGF VM M EAAK GD F+T TG+ IR EH+++MKDGAIM NAGHFDVE+ KP+L Sbjct: 253 ARMDGFLVMNMKEAAKIGDIFITATGDIKCIRREHFELMKDGAIMANAGHFDVEIWKPDL 312 Query: 311 SDRSVSQRTVRKNIEEYQLKDGRKLYLLAEGRLVNLGAADGHPAEIMDTTFALQALSLKY 370 + +V R N+ EY+LKDGR+LYLLA+GRLVNL AADGHPAEIMD +FALQA + +Y Sbjct: 313 EELAVEISNPRPNVTEYKLKDGRRLYLLADGRLVNLVAADGHPAEIMDMSFALQAKAAEY 372 Query: 371 VNDNYKTIGVKVENVPYDLDEQVARFKLESLGISIDSLTPAQVEYLDSW 419 + DN+ + +V +P ++DE VAR KL S+GI I+ LT Q +YL+SW Sbjct: 373 IKDNHGKLEPRVYILPREIDEMVARIKLASMGIEIEELTEEQKKYLESW 421 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.317 0.135 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 619,099,091 Number of extensions: 25740334 Number of successful extensions: 78707 Number of sequences better than 1.0e-05: 992 Number of HSP's gapped: 77438 Number of HSP's successfully gapped: 1092 Length of query: 423 Length of database: 757,237,578 Length adjustment: 128 Effective length of query: 295 Effective length of database: 409,704,906 Effective search space: 120862947270 Effective search space used: 120862947270 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 127 (53.5 bits)