BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0264.0047.p (320 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_388338.1| UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6- diam... 269 7e-71 ref|NP_976594.1| UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diami... 266 6e-70 ref|NP_842799.1| UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diami... 265 1e-69 ref|NP_830126.1| UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diami... 259 4e-68 ref|NP_464382.1| UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diami... 242 7e-63 >ref|NP_388338.1| UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6- diaminopimelate-D-alanyl-D-alanyl ligase [Bacillus subtilis] sp|P96613|MURF_BACSU UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase (UDP-MurNAc-pentapeptide synthetase) (D-alanyl-D-alanine-adding enzyme) pir||F69662 UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate-D-al murF - Bacillus subtilis dbj|BAA19294.1| PROBABLE UDP-N-ACETYLMURAMOYLALANYL-D-GLUTAMYL-2, 6-DIAMINOLIGASE (EC 6.3.2.15). [Bacillus subtilis] emb|CAB12264.1| UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6- diaminopimelate-D-alanyl-D-alanine ligase [Bacillus subtilis subsp. subtilis str. 168] Length = 457 Score = 269 bits (687), Expect = 7e-71, Method: Compositional matrix adjust. Identities = 146/294 (49%), Positives = 185/294 (62%), Gaps = 10/294 (3%) Query: 1 LITRTLRNIAAMCGGELSSAYDMDKSIAGVVTDSRKITPACLFVPLIGDHFDGHDYXXXX 60 +I RT++NIA M G L++ I GV TD+RK+ LF+PL G+ FDGH + Sbjct: 1 MIKRTVKNIAEMVKGTLANPQYEQTVIHGVATDTRKLEQHQLFIPLKGERFDGHSFVEQA 60 Query: 61 XXXXXXXTLWQRDKGAAPENGSVILVDDTLTALQKLASAYLSQVAPQVVAVTGSNGKTTT 120 LW R PEN +VILVDDTLTALQ+LA AYL ++ +V+ VTGSNGKTTT Sbjct: 61 FEAGVAAVLWDRSVPNPPENHAVILVDDTLTALQQLAKAYLQELGTRVIGVTGSNGKTTT 120 Query: 121 KDMITALLEGQYKVHKTQGNFNNHIGLPLTILSMSGDVQIAVLEMGMSSRGEIXXXXXXX 180 KDMI A+L QY+VHKT GNFNNHIGLPLT+L+M + +IAVLEMGMS++GEI Sbjct: 121 KDMIHAVLGTQYRVHKTGGNFNNHIGLPLTVLAMPENTEIAVLEMGMSAKGEIDLLSRLA 180 Query: 181 PPDVAVITNVGESHLLQLGSRKEIARAKLEIAEGLKPGGLLIYNGDEPLLSEVMAESAFR 240 PD AVITN+GESH+ LGSR+ IA AKLEI GLK G+LIY GDEPLL ++A+ Sbjct: 181 NPDAAVITNIGESHMQDLGSREGIAEAKLEIINGLKEDGVLIYIGDEPLL-----QNAYS 235 Query: 241 APEGMRTFRFGLSPDNTDYPXXXXXXXXXXXXXSHLHAEHAFTLPLPGRHNVVN 294 +T +G N DY + E+ F +P+ G+HNV+N Sbjct: 236 C----QTKTYGTGTHN-DYQLQDVSQSEEGTHFTIKGIENTFFIPILGKHNVMN 284 >ref|NP_976594.1| UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanyl ligase [Bacillus cereus ATCC 10987] gb|AAS39202.1| UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanyl ligase [Bacillus cereus ATCC 10987] Length = 458 Score = 266 bits (679), Expect = 6e-70, Method: Compositional matrix adjust. Identities = 139/299 (46%), Positives = 185/299 (61%), Gaps = 6/299 (2%) Query: 1 LITRTLRNIAAMCGGE-LSSAYDMDKSIAGVVTDSRKITPACLFVPLIGDHFDGHDYXXX 59 +I RTL+ + M G L+ Y+ +I GV D+RKI L+VP+ G+ FDGH + Sbjct: 1 MINRTLKQVEQMTNGTGLAEQYE-GITIQGVSIDTRKIEKGNLYVPIQGERFDGHAFVDK 59 Query: 60 XXXXXXXXTLWQRDKGAAPENGSVILVDDTLTALQKLASAYLSQVAPQVVAVTGSNGKTT 119 TLW +D PEN VI V+DTL+ALQ LA Y Q+ +V+ VTGSNGKT+ Sbjct: 60 AVENGAVATLWMKDVANPPENLPVIFVEDTLSALQMLAKNYRDQLDVKVIGVTGSNGKTS 119 Query: 120 TKDMITALLEGQYKVHKTQGNFNNHIGLPLTILSMSGDVQIAVLEMGMSSRGEIXXXXXX 179 TKD++T+LL ++KV KT+GNFNNHIGLPLTIL++ + ++AVLEMGMSSRGEI Sbjct: 120 TKDIVTSLLATKFKVQKTEGNFNNHIGLPLTILNLEENTEVAVLEMGMSSRGEIEFLSKL 179 Query: 180 XPPDVAVITNVGESHLLQLGSRKEIARAKLEIAEGLKPGGLLIYNGDEPLLSEVMAESAF 239 P+ A+ITN+GE+HL+ LGSR+ IA AKLEI GL+ GG+ +YNGDEPLL+ + E Sbjct: 180 ARPNAAIITNIGEAHLMDLGSREAIAEAKLEIVTGLQEGGVFVYNGDEPLLTNRVPEMNL 239 Query: 240 RAPEGMRTFRFGLSPDNTDYPXXXXXXXXXXXXXSHLHAEHAFTLPLPGRHNVVNALAA 298 A T FG + N YP + +F LP+ G+HNV N LA+ Sbjct: 240 IA----ETVTFGDARANDYYPTTVTLQATGTHFKMNREENISFYLPVLGKHNVYNTLAS 294 >ref|NP_842799.1| UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanyl ligase [Bacillus anthracis str. Ames] gb|AAP24285.1| UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanyl ligase [Bacillus anthracis str. Ames] Length = 458 Score = 265 bits (677), Expect = 1e-69, Method: Compositional matrix adjust. Identities = 139/299 (46%), Positives = 185/299 (61%), Gaps = 6/299 (2%) Query: 1 LITRTLRNIAAMCGGE-LSSAYDMDKSIAGVVTDSRKITPACLFVPLIGDHFDGHDYXXX 59 +I RTL+ + M G L+ Y+ +I GV D+RKI L+VP+ G+ FDGH + Sbjct: 1 MINRTLKQVEQMINGTGLAEQYE-GITIQGVSIDTRKIEKGNLYVPIQGERFDGHAFVDK 59 Query: 60 XXXXXXXXTLWQRDKGAAPENGSVILVDDTLTALQKLASAYLSQVAPQVVAVTGSNGKTT 119 TLW +D PEN VI V+DTL+ALQ LA Y Q+ +V+ VTGSNGKT+ Sbjct: 60 AVENGAVATLWVKDVANPPENLPVIFVEDTLSALQMLAKNYRDQLDVKVIGVTGSNGKTS 119 Query: 120 TKDMITALLEGQYKVHKTQGNFNNHIGLPLTILSMSGDVQIAVLEMGMSSRGEIXXXXXX 179 TKD++T+LL ++KV KT+GNFNNHIGLPLTIL++ + ++AVLEMGMSSRGEI Sbjct: 120 TKDIVTSLLATKFKVQKTEGNFNNHIGLPLTILNLEENTEVAVLEMGMSSRGEIEFLSKL 179 Query: 180 XPPDVAVITNVGESHLLQLGSRKEIARAKLEIAEGLKPGGLLIYNGDEPLLSEVMAESAF 239 P+ A+ITN+GE+HL+ LGSR+ IA AKLEI GL+ GG+ +YNGDEPLL+ + E Sbjct: 180 ARPNAAIITNIGEAHLMDLGSREAIAEAKLEIVTGLQEGGVFVYNGDEPLLTNRVPEMNL 239 Query: 240 RAPEGMRTFRFGLSPDNTDYPXXXXXXXXXXXXXSHLHAEHAFTLPLPGRHNVVNALAA 298 A T FG + N YP + +F LP+ G+HNV N LA+ Sbjct: 240 IA----ETVTFGDARANDYYPTTVTLQATGTHFKMNREENISFYLPVLGKHNVYNTLAS 294 >ref|NP_830126.1| UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D- alanyl ligase [Bacillus cereus ATCC 14579] gb|AAP07327.1| UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D- alanyl ligase [Bacillus cereus ATCC 14579] Length = 454 Score = 259 bits (663), Expect = 4e-68, Method: Compositional matrix adjust. Identities = 140/298 (46%), Positives = 183/298 (61%), Gaps = 14/298 (4%) Query: 6 LRNIAAMCGG-ELSSAYDMDKSIAGVVTDSRKITPACLFVPLIGDHFDGHDYXXXXXXXX 64 L+ + M G EL+ Y+ +I GV D+RKI L+VP+ G+ FDGH + Sbjct: 2 LKQVEQMVNGTELAEQYE-GITIQGVSIDTRKIEKGNLYVPIQGERFDGHAFVDKAVENG 60 Query: 65 XXXTLWQRDKGAAPENGSVILVDDTLTALQKLASAYLSQVAPQVVAVTGSNGKTTTKDMI 124 TLW +D PEN VI V+DTL+ALQ LA Y Q+ +VV VTGSNGKT+TKD++ Sbjct: 61 AVATLWMKDVANPPENLPVIFVEDTLSALQMLAKNYRDQLDVKVVGVTGSNGKTSTKDIV 120 Query: 125 TALLEGQYKVHKTQGNFNNHIGLPLTILSMSGDVQIAVLEMGMSSRGEIXXXXXXXPPDV 184 T+LL ++KV KT+GNFNNHIGLPLTIL++ + ++AVLEMGMSSRGEI P+ Sbjct: 121 TSLLATKFKVQKTEGNFNNHIGLPLTILNLEENTEVAVLEMGMSSRGEIEFLSKLARPNA 180 Query: 185 AVITNVGESHLLQLGSRKEIARAKLEIAEGLKPGGLLIYNGDEPLLSEVMAESAFRAPEG 244 A+ITN+GE+HL+ LGSR+ IA AKLEI GL+ GG+ +YNGDEPLL+ R P G Sbjct: 181 AIITNIGEAHLMDLGSREAIAEAKLEIVTGLQEGGVFVYNGDEPLLTN-------RVP-G 232 Query: 245 M----RTFRFGLSPDNTDYPXXXXXXXXXXXXXSHLHAEHAFTLPLPGRHNVVNALAA 298 M T FG + N YP + +F LP+ G+HNV N LA+ Sbjct: 233 MNLAAETVTFGDARANDYYPTTVTLQATGTHFKMNRDENISFYLPVLGKHNVYNTLAS 290 >ref|NP_464382.1| UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diamino pimelate-D-alanyl-D-alanyl ligase [Listeria monocytogenes EGD-e] pir||AH1181 UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diamino pimelate-D-alanyl-D-alanyl ligase [imported] - Listeria monocytogenes (strain EGD-e) emb|CAC98934.1| UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diamino pimelate-D-alanyl-D-alanyl ligase [Listeria monocytogenes] Length = 457 Score = 242 bits (618), Expect = 7e-63, Method: Compositional matrix adjust. Identities = 129/297 (43%), Positives = 172/297 (57%), Gaps = 7/297 (2%) Query: 2 ITRTLRNIAAMCGGELSSAYDMDKSIAGVVTDSRKITPACLFVPLIGDHFDGHDYXXXXX 61 + +T+ + AM + D I GV D+R+I LFVP +G+ DGH++ Sbjct: 1 MKKTVGEVVAMLDSSIHVDAFRDVVITGVCFDTRQIKSGDLFVPFVGNVRDGHEFVGQAR 60 Query: 62 XXXXXXTLWQRDKGAAPENGSVILVDDTLTALQKLASAYLSQVAPQVVAVTGSNGKTTTK 121 T WQ++ P + VILV+DTL ALQ+LA+ Y+ QV P+V+A+TGSNGKTTTK Sbjct: 61 EMGAVATFWQKNVPNPPTDFPVILVEDTLLALQELAAKYIQQVKPKVIAITGSNGKTTTK 120 Query: 122 DMITALLEGQYKVHKTQGNFNNHIGLPLTILSMSGDVQIAVLEMGMSSRGEIXXXXXXXP 181 D++ A++E YKVH T GNFNNHIGLP TIL+M D ++AVLEMGM+ R EI Sbjct: 121 DIMAAIVETTYKVHYTGGNFNNHIGLPYTILTMPEDTEVAVLEMGMNHRHEIEVLSKIAK 180 Query: 182 PDVAVITNVGESHLLQLGSRKEIARAKLEIAEGLKPGGLLIYNGDEPLLSEVMAESAFRA 241 PD+A+ITN+GE+H+ LGSR+EIA+AKLEI GL P G+LIY +E LL Sbjct: 181 PDIAIITNIGEAHIEYLGSREEIAKAKLEITAGLNPSGILIYPHEETLL-------LGNI 233 Query: 242 PEGMRTFRFGLSPDNTDYPXXXXXXXXXXXXXSHLHAEHAFTLPLPGRHNVVNALAA 298 R FG S YP ++ E +P+ G HNV N +AA Sbjct: 234 NGDFRQLTFGKSEAAEIYPLEIRAEAEGTSFITNWEPELEIFVPIIGEHNVFNTMAA 290 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.317 0.134 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 399,241,902 Number of extensions: 14568028 Number of successful extensions: 31741 Number of sequences better than 1.0e-05: 582 Number of HSP's gapped: 31457 Number of HSP's successfully gapped: 582 Length of query: 320 Length of database: 757,237,578 Length adjustment: 125 Effective length of query: 195 Effective length of database: 417,850,203 Effective search space: 81480789585 Effective search space used: 81480789585 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 126 (53.1 bits)