BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0264.0045.p (307 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value sp|Q9K9S6|MRAY_BACHD Phospho-N-acetylmuramoyl-pentapeptide-trans... 298 8e-80 ref|NP_389402.1| phospho-N-acetylmuramoyl-pentapeptide transfera... 285 8e-76 ref|NP_833633.1| Phospho-N-acetylmuramoyl-pentapeptide-transfera... 283 3e-75 ref|NP_980254.1| phospho-N-acetylmuramoyl-pentapeptide-transfera... 283 5e-75 ref|NP_657883.1| Glycos_transf_4, Glycosyl transferase [Bacillus... 280 2e-74 >sp|Q9K9S6|MRAY_BACHD Phospho-N-acetylmuramoyl-pentapeptide-transferase (UDP-MurNAc-pentapeptide phosphotransferase) Length = 325 Score = 298 bits (764), Expect = 8e-80, Method: Compositional matrix adjust. Identities = 146/301 (48%), Positives = 207/301 (68%), Gaps = 5/301 (1%) Query: 1 MDYQLLLLTIAVSFILAVIAAPLFIPLLRRMKFGQQVRDDGPQTHLKKAGTPTMGGIIIM 60 M ++LLLT+ +SF++ VI +P+FIP LRR+KFGQ +R++GP++H KK+GTPTMGG++I+ Sbjct: 1 MLERVLLLTLILSFVITVILSPIFIPFLRRLKFGQSIREEGPKSHQKKSGTPTMGGLMIL 60 Query: 61 VAFTLSFL----KFSVVNSDFYVLLVAALGYGLIGFLDDYIKIAFKRSLGLTPRQKLLGQ 116 ++ +S L + S+ + D +LL+ +G+G++GF+DD+IK+ KR+LGLT +QKL+GQ Sbjct: 61 LSILVSSLFVSFQLSIFSMDVLLLLLVTIGFGVLGFIDDFIKVVMKRNLGLTSKQKLIGQ 120 Query: 117 LLVGAVMCGLLINAGHNTGISIPGTSISFDWGGWFYYPFIIIMMMAVTNAVNFTDGVDGL 176 L+V + L N G +T ++IP TS+S D+G WFY P +I+M++ +NAVN TDG+DGL Sbjct: 121 LVVAVLFYLGLRNMGLSTEVTIPATSLSIDFG-WFYLPLVIVMLVGASNAVNLTDGLDGL 179 Query: 177 LSGXXXXXXXXXXXXXXXXTSXXXXXXXXXXXXXXLGFLVFNAHPAKVFMGDFGSFXXXX 236 ++G LGFLVFN+HPAKVFMGD GS Sbjct: 180 VAGTGAIAFGAFAIIAWATNYFEVAIFSAAVVGAVLGFLVFNSHPAKVFMGDTGSLALGG 239 Query: 237 XXXXXXXVTKSELLFVVIGGVFVIEMLSVVLQVASFKTRGKRIFRMSPIHHHFELGGWSE 296 + K E+L ++IGGVFVIE LSV++QV SFKTRGKRIF+MSP+HHH+EL GWSE Sbjct: 240 AIAAIAIMAKQEILLIIIGGVFVIETLSVIIQVISFKTRGKRIFKMSPLHHHYELSGWSE 299 Query: 297 W 297 W Sbjct: 300 W 300 >ref|NP_389402.1| phospho-N-acetylmuramoyl-pentapeptide transferase [Bacillus subtilis] sp|Q03521|MRAY_BACSU Phospho-N-acetylmuramoyl-pentapeptide-transferase (UDP-MurNAc-pentapeptide phosphotransferase) pir||C47691 phospho-N-acetylmuramoyl-pentapeptide-transferase (EC 2.7.8.13) mraY - Bacillus subtilis emb|CAA78768.1| mraY [Bacillus subtilis] emb|CAB13392.1| phospho-N-acetylmuramoyl-pentapeptide transferase [Bacillus subtilis subsp. subtilis str. 168] Length = 324 Score = 285 bits (729), Expect = 8e-76, Method: Compositional matrix adjust. Identities = 145/301 (48%), Positives = 198/301 (65%), Gaps = 5/301 (1%) Query: 1 MDYQLLLLTIAVSFILAVIAAPLFIPLLRRMKFGQQVRDDGPQTHLKKAGTPTMGGIIIM 60 M Q++L TI + F+++V+ +P+ IP LRR+KFGQ +R++GP++H KK+GTPTMGG++I+ Sbjct: 1 MLEQVILFTILMGFLISVLLSPILIPFLRRLKFGQSIREEGPKSHQKKSGTPTMGGVMII 60 Query: 61 VAFTLSFL----KFSVVNSDFYVLLVAALGYGLIGFLDDYIKIAFKRSLGLTPRQKLLGQ 116 ++ ++ + KFS ++ + +LL LGYGL+GFLDDYIK+ KR+LGLT +QKL+GQ Sbjct: 61 LSIIVTTIVMTQKFSEISPEMVLLLFVTLGYGLLGFLDDYIKVVMKRNLGLTSKQKLIGQ 120 Query: 117 LLVGAVMCGLLINAGHNTGISIPGTSISFDWGGWFYYPFIIIMMMAVTNAVNFTDGVDGL 176 +++ V + T I IPGT +SFD G W Y+ ++ M++ +NAVN TDG+DGL Sbjct: 121 IIIAVVFYAVYHYYNFATDIRIPGTDLSFDLG-WAYFILVLFMLVGGSNAVNLTDGLDGL 179 Query: 177 LSGXXXXXXXXXXXXXXXXTSXXXXXXXXXXXXXXLGFLVFNAHPAKVFMGDFGSFXXXX 236 LSG + LGFLVFN PAKVFMGD GS Sbjct: 180 LSGTAAIAFGAFAILAWNQSQYDVAIFSVAVVGAVLGFLVFNRDPAKVFMGDTGSLALGG 239 Query: 237 XXXXXXXVTKSELLFVVIGGVFVIEMLSVVLQVASFKTRGKRIFRMSPIHHHFELGGWSE 296 +TK E+L V+IGGVFVIE LSV+LQV SFKT GKRIF+MSP+HHH+EL GWSE Sbjct: 240 AIVTIAILTKLEILLVIIGGVFVIETLSVILQVISFKTTGKRIFKMSPLHHHYELVGWSE 299 Query: 297 W 297 W Sbjct: 300 W 300 >ref|NP_833633.1| Phospho-N-acetylmuramoyl-pentapeptide-transferase [Bacillus cereus ATCC 14579] gb|AAP10834.1| Phospho-N-acetylmuramoyl-pentapeptide-transferase [Bacillus cereus ATCC 14579] Length = 324 Score = 283 bits (725), Expect = 3e-75, Method: Compositional matrix adjust. Identities = 149/303 (49%), Positives = 199/303 (65%), Gaps = 9/303 (2%) Query: 1 MDYQLLLLTIAVSFILAVIAAPLFIPLLRRMKFGQQVRDDGPQTHLKKAGTPTMGGIIIM 60 M Q LL+T V+F+++V +PLFIP LR++KFGQ +RD+GP++H KK+GTPTMGGI+I Sbjct: 1 MLEQGLLVTAGVAFLISVALSPLFIPFLRKLKFGQSIRDEGPKSHQKKSGTPTMGGIVIY 60 Query: 61 VAFTLSFL----KFSVVNSDFYVLLVAALGYGLIGFLDDYIKIAFKRSLGLTPRQKLLGQ 116 V+ ++ L KF+ + ++ +LL+ GYGLIGFLDDYIK+ KR+LGLT +QKL+GQ Sbjct: 61 VSMMVTSLIMAIKFNYLGAEVSLLLLVTFGYGLIGFLDDYIKVVKKRNLGLTSKQKLVGQ 120 Query: 117 LLVGAVMCGLLINAGH--NTGISIPGTSISFDWGGWFYYPFIIIMMMAVTNAVNFTDGVD 174 L++ + LI G +T I IPGT + F+ G W Y+ ++ M++ +NAVN TDG+D Sbjct: 121 LVIA--IAFFLIGKGQAFHTYIMIPGTDVKFELG-WAYFVLVLFMLIGGSNAVNLTDGLD 177 Query: 175 GLLSGXXXXXXXXXXXXXXXXTSXXXXXXXXXXXXXXLGFLVFNAHPAKVFMGDFGSFXX 234 GLLSG LGFLVFNA+PAKVFMGD GS Sbjct: 178 GLLSGTAAIAFGAFSIIAVAQEQFGVAIFCMAVVGAVLGFLVFNANPAKVFMGDTGSLAL 237 Query: 235 XXXXXXXXXVTKSELLFVVIGGVFVIEMLSVVLQVASFKTRGKRIFRMSPIHHHFELGGW 294 + K ELL V+IGGVFV+E LSV++QV SFKT GKR+F+MSP+HHH+EL GW Sbjct: 238 GGAIAAVAILLKQELLLVIIGGVFVMETLSVIIQVISFKTTGKRVFKMSPLHHHYELCGW 297 Query: 295 SEW 297 SEW Sbjct: 298 SEW 300 >ref|NP_980254.1| phospho-N-acetylmuramoyl-pentapeptide-transferase [Bacillus cereus ATCC 10987] gb|AAS42862.1| phospho-N-acetylmuramoyl-pentapeptide-transferase [Bacillus cereus ATCC 10987] Length = 324 Score = 283 bits (723), Expect = 5e-75, Method: Compositional matrix adjust. Identities = 149/303 (49%), Positives = 199/303 (65%), Gaps = 9/303 (2%) Query: 1 MDYQLLLLTIAVSFILAVIAAPLFIPLLRRMKFGQQVRDDGPQTHLKKAGTPTMGGIIIM 60 M Q LL+T V+F+++V +PLFIP LR++KFGQ +RD+GP++H KK+GTPTMGGI+I Sbjct: 1 MLEQGLLVTAGVAFLISVALSPLFIPFLRKLKFGQSIRDEGPKSHQKKSGTPTMGGIVIY 60 Query: 61 VAFTLSFL----KFSVVNSDFYVLLVAALGYGLIGFLDDYIKIAFKRSLGLTPRQKLLGQ 116 V+ ++ L KF+ + ++ +LL+ GYGLIGFLDDYIK+ KR+LGLT +QKL+GQ Sbjct: 61 VSMMVTSLIMAIKFNHLGAEVSLLLLVTFGYGLIGFLDDYIKVVKKRNLGLTSKQKLVGQ 120 Query: 117 LLVGAVMCGLLINAGH--NTGISIPGTSISFDWGGWFYYPFIIIMMMAVTNAVNFTDGVD 174 L++ + LI G +T I IPGT + F+ G W Y+ ++ M++ +NAVN TDG+D Sbjct: 121 LVIA--IAFFLIGKGQAFHTYIMIPGTDVKFELG-WAYFVLVLFMLIGGSNAVNLTDGLD 177 Query: 175 GLLSGXXXXXXXXXXXXXXXXTSXXXXXXXXXXXXXXLGFLVFNAHPAKVFMGDFGSFXX 234 GLLSG LGFLVFNA+PAKVFMGD GS Sbjct: 178 GLLSGTAAIAFGAFSIIAVAQEQFGVAIFCMAVVGAVLGFLVFNANPAKVFMGDTGSLAL 237 Query: 235 XXXXXXXXXVTKSELLFVVIGGVFVIEMLSVVLQVASFKTRGKRIFRMSPIHHHFELGGW 294 + K ELL V+IGGVFV+E LSV++QV SFKT GKR+F+MSP+HHH+EL GW Sbjct: 238 GGAIAAVAILLKQELLLVIIGGVFVMETLSVIIQVISFKTTGKRVFKMSPLHHHYELCGW 297 Query: 295 SEW 297 SEW Sbjct: 298 SEW 300 >ref|NP_657883.1| Glycos_transf_4, Glycosyl transferase [Bacillus anthracis A2012] ref|NP_846292.1| phospho-N-acetylmuramoyl-pentapeptide-transferase [Bacillus anthracis str. Ames] gb|AAP27778.1| phospho-N-acetylmuramoyl-pentapeptide-transferase [Bacillus anthracis str. Ames] Length = 324 Score = 280 bits (717), Expect = 2e-74, Method: Compositional matrix adjust. Identities = 145/301 (48%), Positives = 196/301 (65%), Gaps = 5/301 (1%) Query: 1 MDYQLLLLTIAVSFILAVIAAPLFIPLLRRMKFGQQVRDDGPQTHLKKAGTPTMGGIIIM 60 M Q LL+T V+F+++V +PLFIP LR++KFGQ +RD+GP++H KK+GTPTMGGI+I Sbjct: 1 MLEQGLLVTAGVAFLISVALSPLFIPFLRKLKFGQSIRDEGPKSHQKKSGTPTMGGIVIY 60 Query: 61 VAFTLSFL----KFSVVNSDFYVLLVAALGYGLIGFLDDYIKIAFKRSLGLTPRQKLLGQ 116 V+ ++ L KF+ + ++ +LL+ GYGLIGFLDDYIK+ KR+LGLT +QKL+GQ Sbjct: 61 VSMMVTSLIMAIKFNHLGAEVSLLLLVTFGYGLIGFLDDYIKVVKKRNLGLTSKQKLVGQ 120 Query: 117 LLVGAVMCGLLINAGHNTGISIPGTSISFDWGGWFYYPFIIIMMMAVTNAVNFTDGVDGL 176 L++ + +T I IPGT + F+ G W Y+ ++ M++ +NAVN TDG+DGL Sbjct: 121 LVIAIAFFFIGKGQAFHTYIMIPGTDVKFELG-WAYFVLVLFMLIGGSNAVNLTDGLDGL 179 Query: 177 LSGXXXXXXXXXXXXXXXXTSXXXXXXXXXXXXXXLGFLVFNAHPAKVFMGDFGSFXXXX 236 LSG LGFLVFNA+PA+VFMGD GS Sbjct: 180 LSGTAAIAFGAFSIIAVAQEQFGVAIFCMAVVGAVLGFLVFNANPAEVFMGDTGSLALGG 239 Query: 237 XXXXXXXVTKSELLFVVIGGVFVIEMLSVVLQVASFKTRGKRIFRMSPIHHHFELGGWSE 296 + K ELL V+IGGVFV+E LSV++QV SFKT GKR+F+MSP+HHH+EL GWSE Sbjct: 240 AIAAVAILLKQELLLVIIGGVFVMETLSVIIQVISFKTTGKRVFKMSPLHHHYELCGWSE 299 Query: 297 W 297 W Sbjct: 300 W 300 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.330 0.146 0.448 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 368,601,140 Number of extensions: 13273649 Number of successful extensions: 42494 Number of sequences better than 1.0e-05: 794 Number of HSP's gapped: 41331 Number of HSP's successfully gapped: 1032 Length of query: 307 Length of database: 757,237,578 Length adjustment: 125 Effective length of query: 182 Effective length of database: 417,850,203 Effective search space: 76048736946 Effective search space used: 76048736946 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 125 (52.8 bits)