BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0264.0044.p (472 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_846291.1| UDP-N-acetylmuramoylalanine--D-glutamate ligase... 379 e-104 ref|NP_833632.1| UDP-N-acetylmuramoylalanine--D-glutamate ligase... 377 e-103 ref|NP_980253.1| UDP-N-acetylmuramoylalanine--D-glutamate ligase... 372 e-102 ref|NP_389403.1| UDP-N-acetylmuramoylalanine D-glutamate ligase ... 370 e-101 ref|NP_243433.1| UDP-N-acetylmuramoylalanine-D-glutamate ligase ... 359 6e-98 >ref|NP_846291.1| UDP-N-acetylmuramoylalanine--D-glutamate ligase [Bacillus anthracis str. Ames] gb|AAP27777.1| UDP-N-acetylmuramoylalanine--D-glutamate ligase [Bacillus anthracis str. Ames] Length = 450 Score = 379 bits (973), Expect = e-104, Method: Compositional matrix adjust. Identities = 206/472 (43%), Positives = 280/472 (59%), Gaps = 23/472 (4%) Query: 1 MEHPDQYRGKEVVVLGLAKSGVQVAKVLHERGADVTVNDKKERDQSPEASELESLGISVI 60 M+ +++ K ++VLG+AKSG A +L + GA+V VND K ++ A+EL++ G+ V+ Sbjct: 1 MKTVTEFQNKNILVLGIAKSGYAAATLLQKLGANVIVNDGKPLAENVLAAELQAKGMDVV 60 Query: 61 CGGHPEGLIHPGVSLVVKNPGIPYSVPPVQKALELGIEVVTEVEVAYHLCAAPMIGITGS 120 CGGHP L+ +SLVVKNPGIPYS P + A E I +VTEVE+AY + AP +GITGS Sbjct: 61 CGGHPLELLERNISLVVKNPGIPYSNPILVAAKEKQIPIVTEVELAYRISEAPFVGITGS 120 Query: 121 NGKXXXXXWVGRMLDAAGMHPIVAGNIGTPLCQAAQEAHADNWMVVELSSFQLKGTKAFR 180 NGK ML HP++AGNIGT C+ AQ+A + +V ELSSFQL G + F+ Sbjct: 121 NGKTTTTMLTFEMLKEGQKHPVIAGNIGTVACEVAQDAKENEVVVTELSSFQLMGVELFQ 180 Query: 181 PKVGCLLNVAETHLDYHGGMEDYIASKAKLFANQGPSDTAVLNWDDPVCRELVPYIKAGI 240 PK+ LN+ E HLDYHG ++Y +KA +F NQ +D +V+N DD L Y K Sbjct: 181 PKIAAFLNLFEAHLDYHGTKKEYGLAKANIFKNQTENDYSVINADDADVMALSAYSKGQK 240 Query: 241 LPFSMTEELVQGVFVRPAFVPDTEDDLKRMIVYRDYSESETEIAAVDSIGLPGRFNVEXX 300 + FS T+E+ G ++ + Y ++E ++ VD I LPG+ N+E Sbjct: 241 VLFSTTKEIEDGACIKDNAL---------------YFKAE-KVVEVDDIVLPGQHNLENI 284 Query: 301 XXXXXXXXXXXXXXXXXXEVLVNFRGVEHRLEYVAEKAGAAYYNNSKATNSKATSMALAS 360 VL F GV+HRLEYV +YN+SKATN AT AL++ Sbjct: 285 LAAMSIAKLLGVSNEAITAVLKRFTGVKHRLEYVTTINNRKFYNDSKATNMLATEKALSA 344 Query: 361 FRKPVVLIAGGLDRGSDYMELLPVLGGVKALVALGQTRDKLAAVAGMAGVKRIISVDNGE 420 F +P VL+AGGLDRG+++ +L+P VKA+V GQT KL A AG+ I SVD Sbjct: 345 FTQPTVLLAGGLDRGNEFDDLIPYFKNVKAIVTFGQTAPKLVRAAEKAGLDTIESVD--- 401 Query: 421 SAAAVLQEAVREASALAEAGDIVLLSPACASWDMFTSYEERGRIFKEAVHNL 472 L EAV +A A + GD++LLSPACASWD F ++EERG IF +AVH L Sbjct: 402 ----TLDEAVVKAYAHSTDGDVILLSPACASWDQFKTFEERGDIFIQAVHKL 449 >ref|NP_833632.1| UDP-N-acetylmuramoylalanine--D-glutamate ligase [Bacillus cereus ATCC 14579] gb|AAP10833.1| UDP-N-acetylmuramoylalanine--D-glutamate ligase [Bacillus cereus ATCC 14579] Length = 450 Score = 377 bits (967), Expect = e-103, Method: Compositional matrix adjust. Identities = 206/472 (43%), Positives = 280/472 (59%), Gaps = 23/472 (4%) Query: 1 MEHPDQYRGKEVVVLGLAKSGVQVAKVLHERGADVTVNDKKERDQSPEASELESLGISVI 60 M+ +++ K ++VLG+AKSG A +L + GA+V VND K ++ A+EL++ G+ V+ Sbjct: 1 MKTVTEFQNKNILVLGIAKSGYAAATLLQKLGANVIVNDGKPLAENVLAAELQAKGMDVV 60 Query: 61 CGGHPEGLIHPGVSLVVKNPGIPYSVPPVQKALELGIEVVTEVEVAYHLCAAPMIGITGS 120 CGGHP L+ +SLVVKNPGIPYS P + A E I +VTEVE+AY + AP +GITGS Sbjct: 61 CGGHPLELLERNISLVVKNPGIPYSNPILVAAKEKQIPIVTEVELAYRISEAPFVGITGS 120 Query: 121 NGKXXXXXWVGRMLDAAGMHPIVAGNIGTPLCQAAQEAHADNWMVVELSSFQLKGTKAFR 180 NGK ML HP++AGNIGT C+ AQ+A + +V ELSSFQL G + F+ Sbjct: 121 NGKTTTTMLTFEMLKEGQKHPVIAGNIGTVACEVAQDAKENEVVVTELSSFQLMGVELFQ 180 Query: 181 PKVGCLLNVAETHLDYHGGMEDYIASKAKLFANQGPSDTAVLNWDDPVCRELVPYIKAGI 240 PK+ LN+ E HLDYHG ++Y +KA +F NQ +D +V+N DD L Y K Sbjct: 181 PKIAAFLNLFEAHLDYHGTKKEYGLAKANIFKNQTENDYSVINADDADVMALSAYSKGQK 240 Query: 241 LPFSMTEELVQGVFVRPAFVPDTEDDLKRMIVYRDYSESETEIAAVDSIGLPGRFNVEXX 300 + FS T+E+ G ++ + Y + E ++ V I LPG+ N+E Sbjct: 241 ILFSTTKEIEDGACIKDNAL---------------YFKGE-KVIEVSDIVLPGQHNLENI 284 Query: 301 XXXXXXXXXXXXXXXXXXEVLVNFRGVEHRLEYVAEKAGAAYYNNSKATNSKATSMALAS 360 VL F GV+HRLEYV +YN+SKATN AT AL++ Sbjct: 285 LAAMSIAKLLGTSNEAITVVLKRFTGVKHRLEYVTTINNRKFYNDSKATNMLATEKALSA 344 Query: 361 FRKPVVLIAGGLDRGSDYMELLPVLGGVKALVALGQTRDKLAAVAGMAGVKRIISVDNGE 420 F +P+VL+AGGLDRG+++ +L+P VKA+V GQT KL A AG+ I SVD Sbjct: 345 FTQPIVLLAGGLDRGNEFDDLIPYFKNVKAIVTFGQTAPKLVRAAEKAGLDIIESVD--- 401 Query: 421 SAAAVLQEAVREASALAEAGDIVLLSPACASWDMFTSYEERGRIFKEAVHNL 472 L EAV +A A ++ GD+VLLSPACASWD F ++EERG IF +AVH L Sbjct: 402 ----TLDEAVVKAYAHSKDGDVVLLSPACASWDQFKTFEERGDIFIQAVHKL 449 >ref|NP_980253.1| UDP-N-acetylmuramoylalanine--D-glutamate ligase [Bacillus cereus ATCC 10987] gb|AAS42861.1| UDP-N-acetylmuramoylalanine--D-glutamate ligase [Bacillus cereus ATCC 10987] Length = 450 Score = 372 bits (956), Expect = e-102, Method: Compositional matrix adjust. Identities = 204/472 (43%), Positives = 277/472 (58%), Gaps = 23/472 (4%) Query: 1 MEHPDQYRGKEVVVLGLAKSGVQVAKVLHERGADVTVNDKKERDQSPEASELESLGISVI 60 M+ +++ K ++VLG+AKSG A +L + GA+V VND K ++ A+EL++ G+ V+ Sbjct: 1 MKTVTEFQNKNILVLGIAKSGYAAATLLQKLGANVIVNDGKPLAENVLAAELQAKGMDVV 60 Query: 61 CGGHPEGLIHPGVSLVVKNPGIPYSVPPVQKALELGIEVVTEVEVAYHLCAAPMIGITGS 120 CGGHP L+ +SLVVKNPGIPYS P + A E I +VTEVE+AY + AP +GITGS Sbjct: 61 CGGHPLELLERNISLVVKNPGIPYSNPILVAAKEKQIPIVTEVELAYRISEAPFVGITGS 120 Query: 121 NGKXXXXXWVGRMLDAAGMHPIVAGNIGTPLCQAAQEAHADNWMVVELSSFQLKGTKAFR 180 NGK ML HP++AGNIGT C+ AQ+A + +V ELSSFQL G + F+ Sbjct: 121 NGKTTTTMLTFEMLKEGQKHPVIAGNIGTVACEVAQDAKENEVVVTELSSFQLMGVELFQ 180 Query: 181 PKVGCLLNVAETHLDYHGGMEDYIASKAKLFANQGPSDTAVLNWDDPVCRELVPYIKAGI 240 PK+ LN+ E HLDYHG ++Y +KA +F N +D +V+N DD L Y K Sbjct: 181 PKIAAFLNLFEAHLDYHGTKKEYGLAKANIFKNXTENDYSVINADDADVMALSAYSKGQK 240 Query: 241 LPFSMTEELVQGVFVRPAFVPDTEDDLKRMIVYRDYSESETEIAAVDSIGLPGRFNVEXX 300 + FS T+E+ G ++ + Y + E ++ V I LPG+ N+E Sbjct: 241 VLFSTTKEIEDGACIKDNAL---------------YFKGE-KVVEVGDIVLPGQHNLENI 284 Query: 301 XXXXXXXXXXXXXXXXXXEVLVNFRGVEHRLEYVAEKAGAAYYNNSKATNSKATSMALAS 360 VL F GV+HRLEYV +YN+SKATN AT AL++ Sbjct: 285 LAAMSIAKLLGVSNEAITAVLKRFTGVKHRLEYVTTINNRKFYNDSKATNMLATEKALSA 344 Query: 361 FRKPVVLIAGGLDRGSDYMELLPVLGGVKALVALGQTRDKLAAVAGMAGVKRIISVDNGE 420 F +P VL+AGGLDRG+++ +L+P VKA+V GQT KL A AG+ I SVD Sbjct: 345 FTQPTVLLAGGLDRGNEFDDLIPYFKNVKAIVTFGQTAPKLVRAAEKAGLDTIESVD--- 401 Query: 421 SAAAVLQEAVREASALAEAGDIVLLSPACASWDMFTSYEERGRIFKEAVHNL 472 L EAV +A A + GD++LLSPACASWD F ++EERG IF +AVH L Sbjct: 402 ----TLDEAVVKAYAHSTDGDVILLSPACASWDQFKTFEERGDIFIQAVHKL 449 >ref|NP_389403.1| UDP-N-acetylmuramoylalanine D-glutamate ligase [Bacillus subtilis] sp|Q03522|MURD_BACSU UDP-N-acetylmuramoylalanine--D-glutamate ligase (UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase) (D-glutamic acid adding enzyme) pir||D47691 UDP-N-acetylmuramoylalanine-D-glutamate ligase (EC 6.3.2.9) murD - Bacillus subtilis emb|CAA78769.1| murD [Bacillus subtilis] emb|CAB13393.1| UDP-N-acetylmuramoylalanyl-D-glutamate ligase [Bacillus subtilis subsp. subtilis str. 168] Length = 451 Score = 370 bits (950), Expect = e-101, Method: Compositional matrix adjust. Identities = 199/473 (42%), Positives = 283/473 (59%), Gaps = 24/473 (5%) Query: 1 MEHPDQYRGKEVVVLGLAKSGVQVAKVLHERGADVTVNDKKERDQSPEASELESLGISVI 60 ME+ + + ++LGLAKSG A +LHE+G V VND+K +++ A +L GI V+ Sbjct: 1 MENDQFLQKQHFLILGLAKSGYAAASILHEKGIYVAVNDQKPFEENEPAQKLSEKGIEVV 60 Query: 61 CGGHPEGLI-HPGVSLVVKNPGIPYSVPPVQKALELGIEVVTEVEVAYHLCAAPMIGITG 119 CG HP L +++++KNPGIPY VQ+A + GI V TE+E+AY+L +A IGITG Sbjct: 61 CGEHPVSLFDQHQITILIKNPGIPYENIMVQEAEKRGIPVWTEIELAYYLTSAKFIGITG 120 Query: 120 SNGKXXXXXWVGRMLDAAGMHPIVAGNIGTPLCQAAQEAHADNWMVVELSSFQLKGTKAF 179 SNGK + ML A ++AGNIGT + A A D W+V ELSSFQL GT AF Sbjct: 121 SNGKTTTTTLIYEMLKADSQKALIAGNIGTVASEVAYHADGDEWIVTELSSFQLMGTHAF 180 Query: 180 RPKVGCLLNVAETHLDYHGGMEDYIASKAKLFANQGPSDTAVLNWDDPVCRELVPYIKAG 239 RP++ +LNV + HLDYH E+Y +K K++ +Q SD A++N DD L KA Sbjct: 181 RPEISLILNVFDAHLDYHHTRENYEKAKQKVYLHQTASDKAIVNQDDETVVRLAEAGKAE 240 Query: 240 ILPFSMTEELVQGVFVRPAFVPDTEDDLKRMIVYRDYSESETEIAAVDSIGLPGRFNVEX 299 I+PFS+++ L QG +V+ + MI++ + I ++ + LPG N+E Sbjct: 241 IVPFSVSKTLEQGAYVKDS-----------MIMFNGEA-----ILPLEEVVLPGAHNLEN 284 Query: 300 XXXXXXXXXXXXXXXXXXXEVLVNFRGVEHRLEYVAEKAGAAYYNNSKATNSKATSMALA 359 +VL +F GV+HRL+YV G +YN+SKATN ATS AL+ Sbjct: 285 ILAAIAVVKTAGASNEAVKKVLTSFTGVKHRLQYVTTVNGRKFYNDSKATNILATSKALS 344 Query: 360 SFRKPVVLIAGGLDRGSDYMELLPVLGGVKALVALGQTRDKLAAVAGMAGVKRIISVDNG 419 +F KPV+L+AGGLDRG+ + +L P + VKA++ GQT KL + G++ + VDN Sbjct: 345 AFDKPVILLAGGLDRGNGFDDLKPYMKHVKAVLTFGQTAPKLEKLGNELGIQHVKRVDN- 403 Query: 420 ESAAAVLQEAVREASALAEAGDIVLLSPACASWDMFTSYEERGRIFKEAVHNL 472 +++AV A AL+ GD++LLSPACASWD F ++EERG +F +AVH L Sbjct: 404 ------VEQAVSAAFALSNEGDVILLSPACASWDQFKTFEERGDMFIDAVHML 450 >ref|NP_243433.1| UDP-N-acetylmuramoylalanine-D-glutamate ligase [Bacillus halodurans] sp|Q9K9S8|MURD_BACHD UDP-N-acetylmuramoylalanine--D-glutamate ligase (UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase) (D-glutamic acid adding enzyme) pir||G83970 UDP-N-acetylmuramoylalanine-D-glutamate ligase murD [imported] - Bacillus halodurans (strain C-125) dbj|BAB06286.1| UDP-N-acetylmuramoylalanine-D-glutamate ligase [Bacillus halodurans] Length = 450 Score = 359 bits (922), Expect = 6e-98, Method: Compositional matrix adjust. Identities = 200/472 (42%), Positives = 278/472 (58%), Gaps = 24/472 (5%) Query: 1 MEHPDQYRGKEVVVLGLAKSGVQVAKVLHERGADVTVNDKKERDQSPEASELESLGISVI 60 M+H +Q+ K ++VLGLAKSG A++LH+ GA VTVND+K +P+A +L+ GI V+ Sbjct: 1 MKHTEQFHQKHILVLGLAKSGEAAARLLHDLGAIVTVNDQKPLADNPQAQKLQKEGIHVV 60 Query: 61 CGGHPEGLIHPGVSLVVKNPGIPYSVPPVQKALELGIEVVTEVEVAYHLCAAPMIGITGS 120 CG HP L+ G LVVKNPGI Y P V++A++ GI VVTEVE+A + A ++ ITGS Sbjct: 61 CGEHPISLL-DGKELVVKNPGIRYDNPIVEEAIKRGISVVTEVELASKVSEAEIVAITGS 119 Query: 121 NGKXXXXXWVGRMLDAAGMHPIVAGNIGTPLCQAAQEAHADNWMVVELSSFQLKGTKAFR 180 NGK V ML + P VAGNIG A+EA AD+ +V+E+SSFQL GT FR Sbjct: 120 NGKTTTTSLVVEMLKGSAREPKVAGNIGVVASDVAREATADDVIVMEVSSFQLMGTSHFR 179 Query: 181 PKVGCLLNVAETHLDYHGGMEDYIASKAKLFANQGPSDTAVLNWDDPVCRELVPYIKAGI 240 PKV LLN+ + HLDYHG E+Y+A+K K+ N D V N DDP+ ++ KA Sbjct: 180 PKVAILLNIFDAHLDYHGSKENYVAAKKKIVENMKEEDYFVYNADDPLVSKVAAETKATP 239 Query: 241 LPFSMTEELVQGVFVRPAFVPDTEDDLKRMIVYRDYSESETEIAAVDSIGLPGRFNVEXX 300 +PFS + + G +V D E + R +I + LPG NV+ Sbjct: 240 IPFSRSTVVKSGAYV------DGETYMFR----------GEKIVEKGDVVLPGDHNVDNV 283 Query: 301 XXXXXXXXXXXXXXXXXXEVLVNFRGVEHRLEYVAEKAGAAYYNNSKATNSKATSMALAS 360 VL F GVEHRL++V +YN+SKATN +T+ A+ S Sbjct: 284 LAAMSAALLMGATKEQIHHVLSTFSGVEHRLQFVGTAFERKWYNDSKATNILSTTAAIQS 343 Query: 361 FRKPVVLIAGGLDRGSDYMELLPVLGGVKALVALGQTRDKLAAVAGMAGVKRIISVDNGE 420 F P+VL+AGGLDRG+ + +L+P L VKA+V G+T+ KLA A AGV+ I+ + Sbjct: 344 FTDPIVLLAGGLDRGNSFDDLIPALQKVKAVVLFGETKHKLAQAAMEAGVETIVEAER-- 401 Query: 421 SAAAVLQEAVREASALAEAGDIVLLSPACASWDMFTSYEERGRIFKEAVHNL 472 +++AVR+A ++ GD++LLSPACASWD + ++EERG F ++ L Sbjct: 402 -----VEDAVRKALDVSANGDVILLSPACASWDQYRTFEERGEAFVTSIEGL 448 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.317 0.134 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 638,742,817 Number of extensions: 24723156 Number of successful extensions: 74361 Number of sequences better than 1.0e-05: 653 Number of HSP's gapped: 73500 Number of HSP's successfully gapped: 762 Length of query: 472 Length of database: 757,237,578 Length adjustment: 129 Effective length of query: 343 Effective length of database: 406,989,807 Effective search space: 139597503801 Effective search space used: 139597503801 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 128 (53.9 bits)