BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0264.0028.p (64 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_243414.1| BH2548~unknown conserved protein [Bacillus halo... 76 2e-13 ref|ZP_00182134.1| hypothetical protein Exigu257601 [Exiguobacte... 72 6e-12 ref|NP_833618.1| Integral membrane protein [Bacillus cereus ATCC... 71 1e-11 ref|NP_980238.1| ylmG protein [Bacillus cereus ATCC 10987] >gi|4... 69 4e-11 ref|NP_389423.1| similar to hypothetical proteins [Bacillus subt... 66 2e-10 >ref|NP_243414.1| BH2548~unknown conserved protein [Bacillus halodurans] pir||D83968 hypothetical protein BH2548 [imported] - Bacillus halodurans (strain C-125) dbj|BAB06267.1| BH2548~unknown conserved protein [Bacillus halodurans] Length = 84 Score = 76.3 bits (186), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 36/59 (61%), Positives = 44/59 (74%), Gaps = 1/59 (1%) Query: 1 MSWLPNVRDSFIGELLGKLVEPYLTPFRRIIPALFGTIDISPIIALFVLEFAKNGLQYI 59 MSW PN R+S G+ LG +VEPYL PFR+IIP L G IDISPI+A+F L FA+ G+ I Sbjct: 25 MSWFPNARESSFGQFLGSIVEPYLAPFRKIIPPL-GMIDISPIVAIFALTFARYGVHAI 82 >ref|ZP_00182134.1| hypothetical protein Exigu257601 [Exiguobacterium sp. 255-15] Length = 90 Score = 71.6 bits (174), Expect = 6e-12, Method: Compositional matrix adjust. Identities = 32/59 (54%), Positives = 43/59 (72%) Query: 1 MSWLPNVRDSFIGELLGKLVEPYLTPFRRIIPALFGTIDISPIIALFVLEFAKNGLQYI 59 +SW PN R+S G++L LVEP+L PFRRIIP + G +DISPI+A VL A+ G++ I Sbjct: 30 LSWFPNARESRFGQVLAMLVEPFLAPFRRIIPPIGGMLDISPIVAFLVLNLAQAGIRAI 88 >ref|NP_833618.1| Integral membrane protein [Bacillus cereus ATCC 14579] gb|AAP10819.1| Integral membrane protein [Bacillus cereus ATCC 14579] Length = 87 Score = 70.9 bits (172), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 33/62 (53%), Positives = 44/62 (70%), Gaps = 1/62 (1%) Query: 1 MSWLPNVRDSFIGELLGKLVEPYLTPFRRIIPALFGTIDISPIIALFVLEFAKNGLQYIV 60 +SW P ++S GE L ++ EPYL PFRR IP L G IDISPI+A+F L+ AK GL ++ Sbjct: 25 LSWFPGAKESSFGEFLARICEPYLEPFRRFIPPL-GMIDISPIVAIFALKLAKTGLFSLL 83 Query: 61 AY 62 +Y Sbjct: 84 SY 85 >ref|NP_980238.1| ylmG protein [Bacillus cereus ATCC 10987] gb|AAS42846.1| ylmG protein [Bacillus cereus ATCC 10987] Length = 87 Score = 68.6 bits (166), Expect = 4e-11, Method: Compositional matrix adjust. Identities = 32/62 (51%), Positives = 41/62 (66%), Gaps = 1/62 (1%) Query: 1 MSWLPNVRDSFIGELLGKLVEPYLTPFRRIIPALFGTIDISPIIALFVLEFAKNGLQYIV 60 +SW P ++S GE L ++ EPYL PFRR IP L G IDISP++A+F L A NGL + Sbjct: 25 LSWFPGAKESTFGEFLARICEPYLEPFRRFIPPL-GMIDISPLVAIFALRLATNGLVSLF 83 Query: 61 AY 62 Y Sbjct: 84 GY 85 >ref|NP_389423.1| similar to hypothetical proteins [Bacillus subtilis] pir||G69876 conserved hypothetical protein ylmG [imported] - Bacillus subtilis emb|CAB13414.1| ylmG [Bacillus subtilis subsp. subtilis str. 168] Length = 90 Score = 66.2 bits (160), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 29/56 (51%), Positives = 40/56 (71%), Gaps = 1/56 (1%) Query: 1 MSWLPNVRDSFIGELLGKLVEPYLTPFRRIIPALFGTIDISPIIALFVLEFAKNGL 56 MSW+P+ R++ +G L + EPYL PFR+IIP + +DISPI+A+ VL FA GL Sbjct: 27 MSWVPSTRETAVGRFLASICEPYLEPFRKIIPPI-AMLDISPIVAILVLRFATTGL 81 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.331 0.151 0.462 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 97,276,255 Number of extensions: 3159067 Number of successful extensions: 8025 Number of sequences better than 1.0e-05: 15 Number of HSP's gapped: 8063 Number of HSP's successfully gapped: 15 Length of query: 64 Length of database: 757,237,578 Length adjustment: 35 Effective length of query: 29 Effective length of database: 662,209,113 Effective search space: 19204064277 Effective search space used: 19204064277 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 120 (50.8 bits)