BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0264.0004.p (495 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00118948.1| COG4124: Beta-mannanase [Cytophaga hutchinsonii] 158 4e-37 gb|AAC44232.1| beta-1,4-mannanase 154 5e-36 gb|AAB82454.1| beta-mannanase [Dictyoglomus thermophilum] 149 2e-34 gb|AAK19890.1| beta-mannanase [Polyangium cellulosum] 133 1e-29 ref|ZP_00060158.1| COG4124: Beta-mannanase [Clostridium thermoce... 125 2e-27 >ref|ZP_00118948.1| COG4124: Beta-mannanase [Cytophaga hutchinsonii] Length = 815 Score = 158 bits (399), Expect = 4e-37, Method: Compositional matrix adjust. Identities = 91/251 (36%), Positives = 135/251 (53%), Gaps = 20/251 (7%) Query: 115 SAIRWHQAG---GIVTMSYHENLPGTAPAWSNVSMSLSEA-DFAKYITPGTVQYKALIAE 170 +AI W+ + G+V +H + P A +N + S A D K +T GT +Y A+I + Sbjct: 250 AAIDWYNSTCQKGMVAFHWHWHSPSGGQAGTNTFYTNSTAFDVTKAVTQGTPEYTAVIRD 309 Query: 171 LDKTAQSLKKLSNAGVPVLWRPYHEMNGGWFWWGQKSSFS--KLWNLMFDRYTAYHKLHN 228 +D A LK+L AGVPVLWRP HE G WFWWG K + L++++++R YH +HN Sbjct: 310 IDSIATQLKRLQAAGVPVLWRPLHEAGGAWFWWGAKGPAAALALYDMVYNRIVNYHNIHN 369 Query: 229 LLWVWSPNAKNEWSDEPADYYPGAGKVDVLALDIYEADFKQSHH----DAMWNLGRG-KL 283 L+W +WS A++YPG KVD+L D Y + D ++ + G K+ Sbjct: 370 LIW--------QWSTPEANWYPGNSKVDMLGYDSYPGAYNYGTQKLIFDQLYTIVNGQKI 421 Query: 284 IAIGENGELPSPAVLAKTQHKWSYQMSWGKLLYEKNSDAVIKAFMNDSFVLTRKEYAAKA 343 +A+ ENG +P P + + KW+Y MSW L+ ++NS A + N + VLT E A Sbjct: 422 LAMTENGPIPDPDLCFSSDAKWAYVMSWSDLVTQQNSSAQLNLVYNHAKVLTLDE-VTTA 480 Query: 344 AQYASMPEAGP 354 A + AGP Sbjct: 481 TNTAQLTAAGP 491 >gb|AAC44232.1| beta-1,4-mannanase Length = 911 Score = 154 bits (389), Expect = 5e-36, Method: Compositional matrix adjust. Identities = 101/314 (32%), Positives = 157/314 (50%), Gaps = 34/314 (10%) Query: 47 RKLLQTLYRLQGQGMISGQHDYLESPDEFSARLNTTSGQYAALHGYELGAIENXXXXXXX 106 +KL++ L + G+ ++SGQ + E + T +Y A+ G++ ++ Sbjct: 600 QKLMKYLASIYGEKILSGQQSSGDGK-EIQMIFDVTK-RYPAVRGFDF--MDYSPSRVEH 655 Query: 107 XXXXAVVDSAIRWHQAGGIVTMSYHENLPG---TAPA---WSNVSMSLSEADFAKYI-TP 159 V+ AI+W ++GGIV +H N P P W + D K + P Sbjct: 656 GTKGTDVEEAIKWWKSGGIVAFCWHWNAPTGLIDQPGKEWWRGFYTEATTFDIKKAMDNP 715 Query: 160 GTVQYKALIAELDKTAQSLKKLSNAGVPVLWRPYHEMNGGWFWWGQK--SSFSKLWNLMF 217 + +YK ++ ++D A+ LK+L GVPVL+RP HE +GGWFWWG K + KLW LMF Sbjct: 716 NSTEYKLILRDIDAIAEQLKRLQKEGVPVLFRPLHEASGGWFWWGAKGPEPYIKLWKLMF 775 Query: 218 DRYTAYHKLHNLLWVWSPNAKNEWSDEPADYYPGAGKVDVLALDIYEADFKQSHH----- 272 DR YHKL+NL+WV W+ + A +YPG VD++ DIYE + S + Sbjct: 776 DRLVNYHKLNNLIWV--------WNGQDAAWYPGDNYVDIIGEDIYEEKAQYSPYADRFA 827 Query: 273 DAMWNLGRGKLIAIGENGELPSPAVLAKTQHKWS--------YQMSWGKLLYEKNSDAVI 324 A+ K+IA+ E G +P PAVL + WS + M+ K E N + ++ Sbjct: 828 KALKYTNARKMIALTECGTIPDPAVLKQEGISWSWFSVWAGNFVMTGSKYNDEWNDNHML 887 Query: 325 KAFMNDSFVLTRKE 338 + N +V+T+ E Sbjct: 888 RKIYNSDYVITKDE 901 >gb|AAB82454.1| beta-mannanase [Dictyoglomus thermophilum] Length = 398 Score = 149 bits (375), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 96/308 (31%), Positives = 149/308 (48%), Gaps = 27/308 (8%) Query: 47 RKLLQTLYRLQGQGMISGQHDYLESPDEFSARLNTTSGQYAALHGYELGAIENXXXXXXX 106 +KL+ L + G+ +SGQ Y ++ + + ++ A+ G+++ ++ Sbjct: 94 QKLMDYLVSIYGKYTLSGQMGYKDA-----FWIWNITDKFPAICGFDM--MDYSPSRVER 146 Query: 107 XXXXAVVDSAIRWHQAGGIVTMSYHENLPG---TAPA---WSNVSMSLSEADFAKYIT-P 159 V+ AI W GGIV +H N P P W + + D + P Sbjct: 147 GASSRDVEDAIDWWNMGGIVQFQWHWNAPKGLYDTPGKEWWRGFYTNATSFDIEYALNHP 206 Query: 160 GTVQYKALIAELDKTAQSLKKLSNAGVPVLWRPYHEMNGGWFWWGQK--SSFSKLWNLMF 217 + YK +I ++D A LK+L A VP+LWRP HE G WFWWG K + KLW L+F Sbjct: 207 ESEDYKLIIRDIDAIAVQLKRLQEAKVPILWRPLHEAEGRWFWWGAKGPEACKKLWRLLF 266 Query: 218 DRYTAYHKLHNLLWVWSPNAKNEWSDEPADYYPGAGKVDVLALDIYEADFKQSHHDAMWN 277 DR YHK++NL+WVW+ S + +YPG VD++ DIY D S M+ Sbjct: 267 DRLVNYHKINNLIWVWTTTD----SPDALKWYPGDEYVDIVGADIYLKDKDYSPSTGMFY 322 Query: 278 -----LGRGKLIAIGENGELPSPAVLAKTQHKWSYQMSWGKLLYEKNSDAV--IKAFMND 330 G KL+A+ ENG +P P ++ + + W + M+W + N + + IK N Sbjct: 323 NIVKLFGGKKLVALTENGIIPDPDLMKEQKAYWVWFMTWSGFENDPNKNEISHIKKVFNH 382 Query: 331 SFVLTRKE 338 FV+T+ E Sbjct: 383 PFVITKDE 390 >gb|AAK19890.1| beta-mannanase [Polyangium cellulosum] Length = 502 Score = 133 bits (334), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 91/304 (29%), Positives = 145/304 (47%), Gaps = 34/304 (11%) Query: 47 RKLLQTLYRLQGQGMISGQHD--YLESPDEFSARLNTTSGQYAALHGYELGAIENXXXXX 104 R+LL +Y + G ++SGQH+ ++ P+ ++ +G+Y A+ G + G Sbjct: 216 RRLLSYIYSVYGNHILSGQHESTWVGGPEYEMNYIHDRTGKYPAIRGLDFG--------- 266 Query: 105 XXXXXXAVVDSAIRWHQAGGIVTMSYHENLPGTAPAWSNVSMSLSEADFAKYITPGTVQY 164 + AI W AGGI + YH P ++ M++S +T GT + Sbjct: 267 --DSGTSAASRAIAWWNAGGIPMIGYHMGAPTKPDGYAGSQMTVS---INAVLTSGTAEN 321 Query: 165 KALIAELDKTAQSLKKLSNAGVPVLWRPYHEMNGGWFWWGQK--SSFSKLWNLMFDRYTA 222 ++ LD A L+KL +A V VLWRP+HE G WFWW ++ + + +LW MF+ +T Sbjct: 322 RSFHQRLDFAAGELQKLEDANVAVLWRPFHEAGGTWFWWSKEGGAQYVRLWRYMFNYFTR 381 Query: 223 YHKLHNLLWVWSPNAKNEWSDEP-ADYYPGAGKVDVLALDIYEADFKQSHHDAMWNLGRG 281 L NL+W+ + +S +P A +YPG VD+ D Y +D + AM++ RG Sbjct: 382 DKGLTNLVWL------HPYSGDPQASFYPGKEFVDITGADTYVSD--HNPLKAMFDRVRG 433 Query: 282 KL-----IAIGENGELPSPAVLAKTQHKWSYQMSWGK--LLYEKNSDAVIKAFMNDSFVL 334 IA+ ENG +P P L +W +W L N D ++ +V+ Sbjct: 434 IAGTTIPIALHENGPIPDPDQLEAQGARWVLFNTWHTDWLTGGSNPDDFLRKVYGHRYVV 493 Query: 335 TRKE 338 TR E Sbjct: 494 TRDE 497 >ref|ZP_00060158.1| COG4124: Beta-mannanase [Clostridium thermocellum ATCC 27405] Length = 597 Score = 125 bits (314), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 83/269 (30%), Positives = 135/269 (50%), Gaps = 22/269 (8%) Query: 56 LQGQGMISGQHDYLESPDEFSARLNTTSGQYAALHGYELGAIENXXXXXXXXXXXAVVDS 115 L GQ I G H+Y S EF+ + +G+ A+ G++ + Sbjct: 210 LSGQQEICGSHNYPGSEAEFT-YIQEKTGKLPAIRGFDFMNYRGNGLMWDDQ----CAER 264 Query: 116 AIRWH-QAGGIVTMSYHENLPGTAPAWSNVSMSLSEADFA--KYITPGTVQYKALIAELD 172 I W+ + GGI T+ +H PG ++ S F+ + +TPGT + AL+ ++D Sbjct: 265 VIEWYKEKGGIPTVCWHWFSPGDIGKKADNSFYTESTTFSISRALTPGTEENIALLNDID 324 Query: 173 KTAQSLKKLSNAGVPVLWRPYHEMNGGWFWWGQK--SSFSKLWNLMFDRYTAYHKLHNLL 230 A+ LK++ +AGVPVL+RP HE GGWFWWG + +L+ L++D++T + L+NL+ Sbjct: 325 TMARKLKQVQDAGVPVLFRPLHEAEGGWFWWGAEGPEPCVRLYRLLYDKFTNEYGLNNLI 384 Query: 231 WVWSPNAKNEWSDEPADYYPGAGKVDVLALDIYEADFKQSHHDAM----WNLGR----GK 282 WVW+ + A +YPG VD++ D Y A + + A+ +NL + K Sbjct: 385 WVWTSYD----YETSAAWYPGDDVVDIIGYDKYNAKDGKPNGSAISSTFYNLVKLTNGKK 440 Query: 283 LIAIGENGELPSPAVLAKTQHKWSYQMSW 311 L+A+ EN +P + L + W Y W Sbjct: 441 LVAMTENDTIPRVSNLVNEKAGWLYFCPW 469 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.317 0.131 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 739,077,191 Number of extensions: 29791385 Number of successful extensions: 58785 Number of sequences better than 1.0e-05: 63 Number of HSP's gapped: 58689 Number of HSP's successfully gapped: 67 Length of query: 495 Length of database: 757,237,578 Length adjustment: 130 Effective length of query: 365 Effective length of database: 404,274,708 Effective search space: 147560268420 Effective search space used: 147560268420 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)