BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0263.0022.p (324 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00158967.1| hypothetical protein Avar422101 [Anabaena var... 92 1e-17 ref|ZP_00105816.1| COG1404: Subtilisin-like serine proteases [No... 87 5e-16 ref|NP_782883.1| putative serine-type protease [Clostridium teta... 79 1e-13 ref|NP_349839.1| Subtilisin like protease (two fused domains) [C... 79 2e-13 gb|AAD26142.1| serine-type protease [Aquifex pyrophilus] 76 1e-12 >ref|ZP_00158967.1| hypothetical protein Avar422101 [Anabaena variabilis ATCC 29413] Length = 513 Score = 92.4 bits (228), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 103/357 (28%), Positives = 154/357 (43%), Gaps = 53/357 (14%) Query: 1 VFDEAG--ATPCVINISLGTYGGPHDGTSLVEQGIDGLVMEQTNRAVVIAAGNAFDDRIH 58 +F AG P V+NISLG + HDGT + Q ID + + V AAGN + IH Sbjct: 164 IFRVAGELGRPAVVNISLGNHSDAHDGTDSLSQIID--AESGSGKIVCCAAGNEGNYNIH 221 Query: 59 ASGTITEGSFTDLHWEVFEGDTTNNEVEIWY--SGEDEFRIELLN---RDHQSIFNIGVG 113 I G + + V + WY SG+ E ++ N Q I G Sbjct: 222 GQANIPSGRNRGMRFHVPLNQIGIVWLNAWYASSGQLEVSVQSPNGFVSPWQKIITDGNP 281 Query: 114 EKA----DSP-GFFAAHTKGSNGDHQAMI-FSGSGLA----GVWIVRIHGVKAAHGTFHA 163 ++ DS +NGD+ + G G + GVW +R ++ Sbjct: 282 SQSYNLPDSQVEIVTPGPDPANGDYNVFVQIRGQGKSPVQGGVWQLRSRNTSSSDTRLDV 341 Query: 164 WIERDDNRNLHKFNQSRFQSPVDNTCTLGSICCGQKSIVVGSYNGK------DPSL---- 213 W DD ++ +S V + +GS C + ++ V +Y K D + Sbjct: 342 WT-LDDTSSVFFTGKS-----VKDALKIGSPGCAKSAVTVAAYTTKAKFQDIDGQIQEMG 395 Query: 214 ----PISYFSSAGPTRDGRQKPEISAPGHFIIAAC---------SRTINEPKID-SGTSM 259 I+ FSS GP R+ QKP+++APG I++A S TIN I SGTSM Sbjct: 396 LDLNTIADFSSEGPLRNNTQKPDVAAPGAMIVSALSGYAHSDDRSMTINPQFIAMSGTSM 455 Query: 260 AAPVVTGVIALMLAEAVKYDLKLSIDEIRRLLFSTTKRNPPAEQGWNNQYGNGRVNA 316 AAP +TG+IAL+L D + D +R+ S+ PP ++ ++G G +NA Sbjct: 456 AAPFITGLIALLLQRNPSLDPEAIKDLLRQ--SSSIPGKPPGT--FDPKWGFGLINA 508 >ref|ZP_00105816.1| COG1404: Subtilisin-like serine proteases [Nostoc punctiforme] Length = 350 Score = 87.0 bits (214), Expect = 5e-16, Method: Compositional matrix adjust. Identities = 88/346 (25%), Positives = 137/346 (39%), Gaps = 50/346 (14%) Query: 10 CVINISLGTYGGPHDGTSLVEQGIDGLVMEQTNRAVVIAAGNAFDDRIHASGTITEGSFT 69 V+N+SLG + HDG+ + + ID R V AAGN +D IH I G Sbjct: 15 AVVNLSLGGHADAHDGSDSLSKVIDA--ETGPGRIVCCAAGNEGNDNIHGQAIIPSGRTR 72 Query: 70 DLHWEVFEGDTTNNEVEIWYSGEDEFRIELLNRD-----HQSIFNIG-VGEKADSPG--- 120 + + V + WYS + E + + + + Q I G + P Sbjct: 73 GMRFNVPLNQIGIVWLNAWYSKDSELEVSVRSPNGFVTPFQKIITDGNAAQDYQLPDARV 132 Query: 121 -FFAAHTKGSNGDHQAM-----IFSGSGLAGVWIVRIHGVKAAHGTFHAWIERDDNRNLH 174 +NGDH I + GVW +R+ A W DD ++ Sbjct: 133 QLATPAPDKANGDHNFFVQIRGIDPSPVMGGVWQLRVRNSSATDTRLDVWT-LDDTSSVF 191 Query: 175 KFNQSRFQSPVDNTCTLGSICCGQKSIVVGSYNGKDPSL--------------PISYFSS 220 +S V +T +GS +I V +Y K IS FSS Sbjct: 192 FTGKS-----VKDTLKIGSPGAASSAITVAAYTTKTKYTDIDNQVREMGLELHTISEFSS 246 Query: 221 AGPTRDGRQKPEISAPGHFIIAACSRTINEPK---------IDSGTSMAAPVVTGVIALM 271 GP R+ KP+++APG II+ S N + + +GTSMA P V+G++AL+ Sbjct: 247 EGPLRNDSHKPDVAAPGAMIISTLSADANFDRSSMINSKFVVQAGTSMATPFVSGLVALL 306 Query: 272 LAEAVKYDLKLSIDEIRRLLFSTTKRNPPAEQGWNNQYGNGRVNAV 317 L + D L ++ LL + ++ ++G G +NA+ Sbjct: 307 L----QRDPNLEPTAVKDLLRKNSAIPGKPAGTFDEKWGYGVINAL 348 >ref|NP_782883.1| putative serine-type protease [Clostridium tetani E88] gb|AAO36820.1| putative serine-type protease [Clostridium tetani E88] Length = 978 Score = 79.3 bits (194), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 96/322 (29%), Positives = 141/322 (43%), Gaps = 27/322 (8%) Query: 9 PCVINISLGTYGGPHDGTSLVEQGIDGLVMEQTNRAVVIAAGNAFDDRIHASGTITEGSF 68 P VIN+S T G H G+SL EQ I+ V + V+AAGN DR H G I + Sbjct: 66 PLVINLSFSTNDGSHKGSSLFEQYIN-TVCRLEPISFVVAAGNE-GDRAHHVGDIVKEE- 122 Query: 69 TDLHWEVFEGDTTNNEVEIWYSGEDEFRIELLNRDHQS----IFNIGVGEKADSPGFFAA 124 + V +G+ ++I+ + F IE++N S I + G E Sbjct: 123 QSIPINVSQGE-RGLILQIYKDIINNFTIEIINPSSNSSGKIILDEGYKEVNIGRDKLYI 181 Query: 125 HTKGS-----NGDHQAMIFSGSG--LAGVWIVRIHGVKAAHGTFHAWIERDDNRNLHKFN 177 + G NG+ I + L+G+W + I G + W+ ++ N + Sbjct: 182 YNTGPKPYNMNGEIIITILPNNDFILSGIWKLNITCKDEYKGKYDIWMPISESLNPN--- 238 Query: 178 QSRFQSPVDNTCTLGSICCGQKSIVVGSYNGKDPSLPISYFSSAGPTRD-GRQKPEISAP 236 ++F P + TLG + I VGSYN + IS FS G + KP+I AP Sbjct: 239 -TKFLEP-NVYNTLGIPATVENVISVGSYNYLTNT--ISSFSGRGSLYEKAYLKPDIVAP 294 Query: 237 GHFIIAACSRTINEPKIDSGTSMAAPVVTGVIALMLAEAVKY--DLKLSIDEIRRLLFST 294 G I+++ R + K SGTSMAAP V G+ AL + V D L D ++ L Sbjct: 295 GEEILSSVPRGRFDTK--SGTSMAAPQVAGICALFMQWGVLQGRDPFLYGDRLKYYLLKG 352 Query: 295 TKRNPPAEQGWNNQYGNGRVNA 316 +RN P N +G G + A Sbjct: 353 ARRNRPNLNYPNETWGYGEICA 374 Score = 61.6 bits (148), Expect = 2e-08, Method: Compositional matrix adjust. Identities = 84/319 (26%), Positives = 131/319 (41%), Gaps = 37/319 (11%) Query: 9 PCVINISLGTYGGPHDGTSLVEQGIDGLVMEQTNRAVVIAAGNAFDDRIHASGTITE-GS 67 P VI I LG+ GGPHDG +++E+ ID + AVV GN + H SGT+ + G Sbjct: 643 PMVIYIPLGSNGGPHDGNTILEKYIDN-ISNIRGLAVVTGTGNQGESSTHTSGTLQKSGD 701 Query: 68 FTDLHWEV--FEGDTTNNEVEIWYSGEDEFRIELLNRDHQSIFNIG--VGEKADSPGFFA 123 + +V FE D T IW D+ I + + ++I + + EK + F Sbjct: 702 IKTIELKVGPFEKDLT---FGIWCKKPDKVSIGITSPAGENIEKVSAKIKEKEEIDLVFE 758 Query: 124 AHT--------KGSNGDHQAMIFSGSGLAGVWIVRIHGVKAAHGTFHAWIERDDNRNLHK 175 + GD ++ + G+W + G +G + AW+ ++ + K Sbjct: 759 GSKVFIEYYLPEELTGDELILVKIENIREGIWRFNLIGDLIVNGRYDAWLPQEP--IIDK 816 Query: 176 FNQSRFQSPVDNTCTLGSICCGQKSIVVGSYNGKDPSLPISYFSSAGPTRDGRQKPEISA 235 Q +P T+ + + YN + S I SS G TRDGR P+I+ Sbjct: 817 ETQFLNSTPY---TTIQMPSSSKSILSTSFYNQNNNS--ILGESSRGYTRDGRIVPDITT 871 Query: 236 PGHFI--IAACSRTINEPKIDSGTSMAAPVVTGVIALMLA-------EAVKYDLKLSIDE 286 G I I R + + SG+S A+ + G ALM + Y +KL Sbjct: 872 GGLNIKTIDNSGRVV----MVSGSSAASAIYAGACALMFQWGIVEGNDPTLYAVKLKTYF 927 Query: 287 IRRLLFSTTKRNPPAEQGW 305 IR + P E G+ Sbjct: 928 IRGATRRESDSYPNPEWGY 946 >ref|NP_349839.1| Subtilisin like protease (two fused domains) [Clostridium acetobutylicum] pir||H97298 subtilisin like protease (two fused domains) CAC3245 [imported] - Clostridium acetobutylicum gb|AAK81179.1| Subtilisin like protease (two fused domains) [Clostridium acetobutylicum] Length = 1118 Score = 78.6 bits (192), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 86/319 (26%), Positives = 148/319 (46%), Gaps = 26/319 (8%) Query: 9 PCVINISLGTYGGPHDGTSLVEQGIDGLVMEQTNRAVVIAAGNAFDDRIHASGTITEGSF 68 P VIN+S T G HDGTSL+EQ I+ + + + + VI+AGN D H G + E Sbjct: 207 PLVINLSFSTNDGAHDGTSLLEQYIENICLLE-KISFVISAGNEGDMGHHVGGVLRESQT 265 Query: 69 TDLHWEVFEGDTTNNEVEIWYSGEDEFRIELLNRDHQSIFNIGVGE-----KADSPGFFA 123 ++ + EG+ + ++++ S + +E+ N + S I + E + + +F Sbjct: 266 ISMN--IAEGERS-IILQLYKSFTQDIAVEVKNPANVSTGVINIKEGYNEGRIQNDRYFI 322 Query: 124 AHTKGS----NGDHQAMIFSGSGL--AGVWIVRIHGVKAAHGTFHAWIERDDNRNLHKFN 177 ++ NG+ + S + + G W++ I G + W+ + N Sbjct: 323 YYSGPKPFSLNGEILINLVSDNEILTTGTWMITITKRSKTVGNYDIWMPVTEGLN----P 378 Query: 178 QSRFQSPVDNTCTLGSICCGQKSIVVGSYNGKDPSLPISYFSSAGPTRDGRQKPEISAPG 237 ++RF P + TLG + ++ VGSYN + IS FS G KP++ APG Sbjct: 379 KTRFLRP-NVYDTLGIPGTVRDAVTVGSYNYLTGA--ISSFSGRGRETGNPIKPDVIAPG 435 Query: 238 HFIIAACSRTINEPKIDSGTSMAAPVVTGVIALMLAEAV--KYDLKLSIDEIRRLLFSTT 295 I ++ + SGTSMA+P V+G+ AL++ + K D+ L D ++ L + Sbjct: 436 ENIESSTPGGGYDAL--SGTSMASPTVSGICALLMQWGIVLKKDIYLYGDRLKYYLLNGA 493 Query: 296 KRNPPAEQGWNNQYGNGRV 314 K++ N +G G V Sbjct: 494 KKDREDITYPNPSFGYGAV 512 Score = 73.9 bits (180), Expect = 4e-12, Method: Compositional matrix adjust. Identities = 82/299 (27%), Positives = 123/299 (41%), Gaps = 33/299 (11%) Query: 9 PCVINISLGTYGGPHDGTSLVEQGIDGLVMEQTNRAVVIAAGNAFDDRIHASGTITEGSF 68 P VI I LGT G HDG +L+E+ ID + AVV AGN D H SGT+ + Sbjct: 782 PIVIYIPLGTNEGGHDGNTLIERYIDQ-ISRVRGVAVVCGAGNEGDTNTHTSGTLRNTND 840 Query: 69 TDLHWEVFEGDTTNN-EVEIWYSGEDEFRIELLNRDHQSIFNIGVGEKADSPGFFAAHTK 127 ++ EV G N + IW + D I + + + + I K P + Sbjct: 841 VNV-IEVKSGQNQKNLPINIWINSPDRVSISITSPSGEIVEKI--PPKYKGPSVTRLVYE 897 Query: 128 GS------------NGDHQAMIFSGSGLAGVWIVRIHGVKAAHGTFHAWIERDDNRNLHK 175 GS GD ++ G+W + + G +G ++AW+ + + Sbjct: 898 GSTVTVEYHFPEALTGDELIRVYIQDIKPGIWKLNLTGEYVQNGQYNAWLP----QKVLL 953 Query: 176 FNQSRFQSPVDNTCTLGSICCGQKSIVVGSYNGKDPSLPISYFSSAGPTRDGRQKPEISA 235 + F +P TL Q I YN + +L S S G TRD R P I+A Sbjct: 954 EEGTEFLNP-SQYITLTVPSTSQSVIACSYYNQDNNTLMSS--SGRGYTRDNRISPIIAA 1010 Query: 236 PGHFIIAACSRTINEPKIDSGTSMAAPVVTGVIALMLAEAVKYDLKLSIDEIRRLLFST 294 G+ + +P +G+S AA V+ G + LML + +D R L+ST Sbjct: 1011 GGYMVPTVDGD--GQPVTLTGSSPAAAVLAGAVILMLQWGI-------VDGNDRTLYST 1060 >gb|AAD26142.1| serine-type protease [Aquifex pyrophilus] Length = 619 Score = 75.9 bits (185), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 100/346 (28%), Positives = 150/346 (43%), Gaps = 59/346 (17%) Query: 4 EAGATPCVINISLGTYGGPHDGTSLVEQGIDGLVMEQTNRAVVIAAGNAFDDRIHASGTI 63 +A P VIN+SLGT GPHDGT + + I+ +V VV+AAGN ++ IH + Sbjct: 235 KAYGKPGVINLSLGTPLGPHDGTDPISRFIESIV-NTYKIPVVVAAGNWGNEPIHDKLNL 293 Query: 64 TEGSFTDLHWEVFEGDTTNNEVEIWYSGEDEFRIELLNRDHQSIFNIGVGEKA----DSP 119 + + + F+ + V+IW G D + +N Q++ +I G+ + Sbjct: 294 FQENTSS-----FDVIGSWAYVDIWIMGTDSVELT-INSPCQAV-SIREGDTKLIDLGNC 346 Query: 120 GFFAAHTKGS---NGDHQAMIF---SGSGLAGVWIVRIHGVKAAHGTFHAWIERDDNRNL 173 G A G NGD I W V + G H W E Sbjct: 347 GTLAVSFSGRNPLNGDKNVFIKIDNVNENFRNGWSVSFTPIDVKDGEVHLWGE------- 399 Query: 174 HKFNQSRFQSPVDNTCTLGSICCGQKSIVVGSYNGK-----DPSL---PISYFSSAGPTR 225 F SP D + T+ S+ I VGS+ K +P IS FSS GP+R Sbjct: 400 ----NVAFASP-DYSYTISSLASSSSVISVGSFTSKVNVNINPYSILGSISNFSSIGPSR 454 Query: 226 ------DGRQKPEISAPGHFIIAACSRTINEPKID----------SGTSMAAPVVTGVIA 269 KP+I APG + +A I +P ID GTSM+AP VTG +A Sbjct: 455 RCSYGCKENLKPDIVAPGELVCSAYPLNI-KPLIDLCFYEGFNWMQGTSMSAPAVTGAVA 513 Query: 270 LMLAEAVKYDLKLSIDEIRRLLFSTTKRNPPAEQGWNNQYGNGRVN 315 L+L + + L+ E++ +L + ++ Q NN YG+G+++ Sbjct: 514 LLL----QVEHTLTPSEVKEILINNAYKDSFTTQKPNNVYGHGKLD 555 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.317 0.134 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 528,872,466 Number of extensions: 22889477 Number of successful extensions: 57789 Number of sequences better than 1.0e-05: 39 Number of HSP's gapped: 57937 Number of HSP's successfully gapped: 41 Length of query: 324 Length of database: 757,237,578 Length adjustment: 126 Effective length of query: 198 Effective length of database: 415,135,104 Effective search space: 82196750592 Effective search space used: 82196750592 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 126 (53.1 bits)