BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0263.0003.p (570 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|AAQ05801.1| N-acetyl-beta-glucosaminidase [Cellulomonas fimi] 603 e-171 ref|ZP_00123196.1| COG1472: Beta-glucosidase-related glycosidase... 469 e-131 ref|NP_561070.1| probable beta-hexosamidase A [Clostridium perfr... 443 e-123 ref|NP_735128.1| Unknown [Streptococcus agalactiae NEM316] >gi|2... 395 e-108 ref|NP_687720.1| glycosyl hydrolase, family 3 [Streptococcus aga... 395 e-108 >gb|AAQ05801.1| N-acetyl-beta-glucosaminidase [Cellulomonas fimi] Length = 564 Score = 603 bits (1555), Expect = e-171, Method: Compositional matrix adjust. Identities = 292/562 (51%), Positives = 380/562 (67%), Gaps = 1/562 (0%) Query: 9 LREKPFYLSEEDVKWVRAAFGQMTVQEKIGQLFFMVGYKQDEPFLKHIAQEIGIGGLMCR 68 L PF L ++ + WVR +M EK+GQLF ++ Y D +L ++ + + +GG+M R Sbjct: 4 LTAAPFSLDDDGIAWVRTTLAEMGEDEKLGQLFCLITYTSDPEYLGYLTRGLHVGGVMLR 63 Query: 69 SMAKEEVVATVATLQRSARIPLLIAANLEAGGQGITRDGTKIGSNMAIAATGSSDLAYQL 128 +M + ATV TLQ +A +PLLI+ANLE G ++ T +GSNMA+AATGS+D + Sbjct: 64 TMTAADAAATVTTLQSTATVPLLISANLEGGASQTVQEATHVGSNMALAATGSTDHVRRA 123 Query: 129 GKICAVEGGSVGANYAFAPVVDIDYNFRNPITNTRTFGSSPQVVMEMGAAYVKGCQEQGM 188 + E ++G N+AF PVVDID NFRNPITNTRTFG+ V MGA YV+ Q QG+ Sbjct: 124 ATVIGREARALGINWAFTPVVDIDLNFRNPITNTRTFGADAATVAAMGAEYVEAIQAQGL 183 Query: 189 AASVKHFPGDGVDERDQHLVTSVNSLTPEAWDDTYGMVYRHLIEEGAKTVMVGHIALPEY 248 AAS KHFPGDGVDERDQHL+ SVN+++ E WDD++G+VYR I G KTVMVGHI LP Y Sbjct: 184 AASAKHFPGDGVDERDQHLLASVNTMSVEEWDDSFGVVYRAAIAAGVKTVMVGHIMLPAY 243 Query: 249 SKLLNPSLRDEEILPASLASXXXXXXXXXXXXFNGLIITDATTMAGMNIPLPRNELVPRA 308 S+ L P + D +ILP +A FNGL+++D+TTMAG+ LPR++ VPR Sbjct: 244 SRALRPGVADRDILPGVVAEELLNDLLRDRLGFNGLVVSDSTTMAGLASVLPRSQAVPRV 303 Query: 309 IENGCDMFLFTKNLEEDVRFMKEGLEAGWLSPGRLDDAVLRILGLKASLKLHERENVPVL 368 I GCDMFLFTKNL+ED +M+ G+ G ++P RLD+AV RIL LKASL LH N+P Sbjct: 304 IAAGCDMFLFTKNLDEDFGYMRAGIRDGVITPERLDEAVTRILALKASLGLHRGTNLPAQ 363 Query: 369 AESSAVLRQDSHLEIARAVADQSITLVKEEAGVLPLSPGRYPRVLLYDIESEANALGYSK 428 ++ VL H AR VA SITLVKEE GVLP++ RYPRVL+YD+++ + +G Sbjct: 364 G-AAGVLADPDHSATAREVAASSITLVKEEPGVLPITRERYPRVLVYDLQNGGSPIGQGA 422 Query: 429 TNGLAAAFIPLLENEGFKVTRFEPAGVYEGLQSSFTEMKESYDLILYLCNLATKSNQTTV 488 G F+ L G VTRFEP G +EG+ + T++ E +DL+LYL NL+T+SNQT V Sbjct: 423 RAGAVEQFVDALVEAGHDVTRFEPGGGWEGMAAPTTDVTERHDLVLYLANLSTRSNQTVV 482 Query: 489 RIEWLNPMGLNVPNYIHSIPTLFISTENPYHLLDVPRVKTYINTYGVNDYTLPQLLAKLM 548 RIEW PMG NVP Y+HS+PT+F+S ENPYHL DVPRV+T INTYG + L LLA L Sbjct: 483 RIEWAEPMGANVPAYVHSVPTVFVSFENPYHLFDVPRVRTLINTYGSSPVVLETLLAALQ 542 Query: 549 GRSQFTGKSPIDPFCGKWDTRL 570 G++ F G SP+D FCG+WDT L Sbjct: 543 GKAPFAGSSPVDAFCGQWDTHL 564 >ref|ZP_00123196.1| COG1472: Beta-glucosidase-related glycosidases [Haemophilus somnus 129PT] Length = 586 Score = 469 bits (1208), Expect = e-131, Method: Compositional matrix adjust. Identities = 246/585 (42%), Positives = 347/585 (59%), Gaps = 26/585 (4%) Query: 9 LREKPFYLSEEDVKWVRAAFGQMTVQEKIGQLFFMVGYKQDEPFLKHIAQEIGIGGLMCR 68 L +KP+YL +ED++WV+ MT++EKIGQLF +G + E +L + IG + Sbjct: 5 LTKKPYYLKDEDIQWVKNTIANMTLEEKIGQLFVNMGSSRSEEYLTDVLNRYHIGAVRYN 64 Query: 69 SMAKEEVVATVATLQRSARIPLLIAANLEAGGQGITRDGTKIGSNMAIAATGSSDLAYQL 128 EEV LQ ++IPLLIAAN EAGG G DGT+IG + I AT + AY++ Sbjct: 65 PGPAEEVYDQNYILQTKSKIPLLIAANTEAGGNGACSDGTEIGLQVKIGATDDAKYAYEM 124 Query: 129 GKICAVEGGSVGANYAFAPVVDIDYNFRNPITNTRTFGSSPQVVMEMGAAYVKGCQEQGM 188 G++ +E ++G N++FAP+VDI YN+RNPI + R FGS+P+ V+EM AY+KG QE G+ Sbjct: 125 GRVAGIEAAAIGCNWSFAPIVDISYNWRNPIISNRVFGSNPEKVLEMSLAYMKGIQESGI 184 Query: 189 AASVKHFPGDGVDERDQHLVTSVNSLTPEAWDDTYGMVYRHLIEEGAKTVMVGHIALPEY 248 A + KHFPGDGVDERDQHL SVNS + E WD+T+G VY+ LI+ G ++M GHI LP Y Sbjct: 185 APAAKHFPGDGVDERDQHLSFSVNSFSCEEWDNTFGKVYQGLIDAGLPSLMAGHIHLPAY 244 Query: 249 SKLLNPSLRDEEILPASLASXXXXXXXXXXXXFNGLIITDATTMAGMNIPLPRNELVPRA 308 + NP+L E+ LPA+L+ FNG+++TDA+ M M + R+E++P A Sbjct: 245 EQHFNPNLAYEDCLPATLSKPILTDLLRGKLGFNGVVVTDASHMVAMTSAMKRSEMLPTA 304 Query: 309 IENGCDMFLFTKNLEEDVRFMKEGLEAGWLSPGRLDDAVLRILGLKASLKLHERENVPVL 368 I GCD+FLF + +ED +M EG + G ++ RL DA+ RILGLKA L LH R +L Sbjct: 305 IAAGCDLFLFFNDPDEDFGYMMEGYKNGIITEERLHDALTRILGLKAKLGLHNRPRETLL 364 Query: 369 AESSAVLRQ---DSHLEIARAVADQSITLVKEEAGVLPLSPGRYPRVLLYDIESEANALG 425 L + H I R VAD++ITLVK + + P++ R+PR+LL +++ G Sbjct: 365 EPKEQALAKIGLPEHKAIFRDVADKAITLVKHKQDIFPINVERFPRILLVNVKGTDGGFG 424 Query: 426 YSKTNGLAAAFIPL---LENEGFKVTRF-------------EPAGVYEGLQSS---FTEM 466 +A L LE +GF V+ + E S T + Sbjct: 425 KMVAGSQRSATEILKEKLEEKGFTVSIYTSPMDNILKMSDEEQVKTIRNEYSQKRPITTL 484 Query: 467 KESYDLILYLCNLATKSNQTTVRIEWLNPMGL-NVPNYIHSIPTLFISTENPYHLLDVPR 525 + YDLI+ N+A T RI W G ++P Y+H +PT+F+S + P+HL+DVP+ Sbjct: 485 TDHYDLII---NVANVQMSTVQRIVWQATKGTPDIPFYVHEVPTIFVSVQCPFHLVDVPQ 541 Query: 526 VKTYINTYGVNDYTLPQLLAKLMGRSQFTGKSPIDPFCGKWDTRL 570 VKTYIN Y + T+ L+ KLMG S+F G SP+D FCG DTR+ Sbjct: 542 VKTYINAYDGKEPTIELLVEKLMGNSEFKGISPVDAFCGYKDTRI 586 >ref|NP_561070.1| probable beta-hexosamidase A [Clostridium perfringens] dbj|BAB79860.1| probable beta-hexosamidase A [Clostridium perfringens str. 13] Length = 589 Score = 443 bits (1139), Expect = e-123, Method: Compositional matrix adjust. Identities = 235/589 (39%), Positives = 342/589 (58%), Gaps = 32/589 (5%) Query: 9 LREKPFYLSEEDVKWVRAAFGQMTVQEKIGQLFFMVGYKQDEPFLKHIAQEIGIGGLMCR 68 L+ KP+ L EE +KWV M+++EKIGQLF +G +DE +LK + IG + Sbjct: 4 LKVKPYNLDEESIKWVEDTIKNMSIEEKIGQLFVNMGASRDEEYLKSVLNNYHIGAVRYN 63 Query: 69 SMAKEEVVATVATLQRSARIPLLIAANLEAGGQGITRDGTKIGSNMAIAATGSSDLAYQL 128 EEV LQ +++IPLLIAAN EAGG G DGT +G+ + IAAT AY+L Sbjct: 64 PGKAEEVYEQNRILQENSKIPLLIAANTEAGGNGACTDGTYVGNEVKIAATQDPHYAYEL 123 Query: 129 GKICAVEGGSVGANYAFAPVVDIDYNFRNPITNTRTFGSSPQVVMEMGAAYVKGCQEQGM 188 G+I +E ++G N++FAP+VDI+ N+RNPI +TRT+ + +E+ Y++G E G+ Sbjct: 124 GRISGIEASAIGCNWSFAPIVDINRNWRNPIISTRTWSADADQTLELSLEYMRGIMESGI 183 Query: 189 AASVKHFPGDGVDERDQHLVTSVNSLTPEAWDDTYGMVYRHLIEEGAKTVMVGHIALPEY 248 + KHFPGDG+DERDQHL + N+L+ E W+ T+G VY LIE G ++M GHIALPEY Sbjct: 184 LPAAKHFPGDGIDERDQHLSFAPNTLSVEEWNATFGKVYSGLIEAGLPSIMAGHIALPEY 243 Query: 249 SKLLNPSLRDEEI-LPASLASXXXXXXXXXXXXFNGLIITDATTMAGMNIPLPRNELVPR 307 K NP +E+ +PA+L+ FNGL++TDA+ M M + R +++P Sbjct: 244 VKYFNPDATIKEMNMPATLSKYILTDLLKGQMGFNGLVVTDASHMVAMTSAMKRKDMLPT 303 Query: 308 AIENGCDMFLFTKNLEEDVRFMKEGLEAGWLSPGRLDDAVLRILGLKASLKLHERENVPV 367 AI G D+FLF + +ED ++M EG + G ++ RL DA+ RILGLKASL LH++ + Sbjct: 304 AIAAGSDLFLFFNDPDEDFQWMMEGYKNGVITEERLHDALTRILGLKASLGLHKKAKTEI 363 Query: 368 LA---ESSAVLRQDSHLEIARAVADQSITLVKEEAGVLPLSPGRYPRVLLYDIESEANAL 424 L E+ A + + + + A +AD++ITLVK + P+SP + RVLL ++ Sbjct: 364 LQPKEEAMAKIGLEENKKAALEIADKAITLVKNNEEIFPISPDKTKRVLLVKVKGFDGGF 423 Query: 425 G------YSKTNGLAAAFIPLLENEGFKVTRFEP-----------------AGVYEGLQS 461 G G LLE EGF+V +E +Y G + Sbjct: 424 GKFLALMQGAQKGPVDIMKELLEQEGFQVEIYESPVDKIMKLPKEEMGAAIGNIYAG-KR 482 Query: 462 SFTEMKESYDLILYLCNLATKSNQTTVRIEWLNPMGL-NVPNYIHSIPTLFISTENPYHL 520 E+ YDLI+ L ++ +Q R++W G ++P Y++ IPT+FIS + P+HL Sbjct: 483 PIAELTSKYDLIINLADVGASVDQ---RLQWPPSKGTPDIPFYVNEIPTIFISLQCPFHL 539 Query: 521 LDVPRVKTYINTYGVNDYTLPQLLAKLMGRSQFTGKSPIDPFCGKWDTR 569 DVP+VKTYIN Y ++ + L+ K+MGRS+F G S +D FCG DTR Sbjct: 540 ADVPQVKTYINAYDSKEFNIKALVDKMMGRSEFKGVSSVDAFCGLKDTR 588 >ref|NP_735128.1| Unknown [Streptococcus agalactiae NEM316] emb|CAD46322.1| Unknown [Streptococcus agalactiae NEM316] Length = 596 Score = 395 bits (1016), Expect = e-108, Method: Compositional matrix adjust. Identities = 220/590 (37%), Positives = 331/590 (56%), Gaps = 32/590 (5%) Query: 9 LREKPFYLSEEDVKWVRAAFGQMTVQEKIGQLFFMVGYKQDEPFLKHIAQEIGIGGLMCR 68 L +KPF L++E ++W+ +MT+ EKIGQLFF +G + E +L + I + Sbjct: 7 LTKKPFNLNQEAIEWIEKTINEMTLDEKIGQLFFNMGASRSEEYLTDVLDRYHIAAVRYN 66 Query: 69 SMAKEEVVATVATLQRSARIPLLIAANLEAGGQGITRDGTKIGSNMAIAATGSSDLAYQL 128 + E+ LQ +++P+LIAAN EAGG G DGTK+G + +AAT AY++ Sbjct: 67 RGSSSEIYDQNLILQTKSKLPMLIAANTEAGGDGAVTDGTKVGDEIKVAATNDPKYAYEM 126 Query: 129 GKICAVEGGSVGANYAFAPVVDIDYNFRNPITNTRTFGSSPQVVMEMGAAYVKGCQEQGM 188 G+I +E +VG N +FAP+VD+ N+RNPI +R +G++ ++ + Y+KG + + Sbjct: 127 GRIAGMEASAVGCNASFAPIVDLTRNWRNPIIASRNWGANVDQIISLSKEYMKGIMQYNI 186 Query: 189 AASVKHFPGDGVDERDQHLVTSVNSLTPEAWDDTYGMVYRHLIEEGAKTVMVGHIALPEY 248 KHFPGDG+DERD HL + N ++ E W T+G +Y L + G VM GHI LP Sbjct: 187 VPFAKHFPGDGIDERDHHLSFASNPMSKEEWMSTFGRIYGELADAGLPGVMAGHIHLPNV 246 Query: 249 SKLLNPSLRDEEILPASLASXXXXXXXXXXXXFNGLIITDATTMAGMNIPLPRNELVPRA 308 K ++P +++LPASL +NG I+TDA+ M GM + R +L+P A Sbjct: 247 EKEMHPERDLDDMLPASLNKTLLDELLRGELGYNGAIVTDASHMVGMTASMSRRDLLPTA 306 Query: 309 IENGCDMFLFTKNLEEDVRFMKEGLEAGWLSPGRLDDAVLRILGLKASLKLHEREN---- 364 IE GCD+FLF + +ED+++MKEG E G L+ RL DA+ R LGLKA L LH E Sbjct: 307 IEAGCDLFLFFNDPDEDIQWMKEGYEKGILTEERLHDALRRTLGLKAKLGLHNYEGRRQE 366 Query: 365 --VPVLAESSAVLRQDSHLEIARAVADQSITLVKE-EAGVLPLSPGRYPRVLLYDIESEA 421 +P ++ A++ +IA VAD+++TLVK+ + + P++P RY +LL ++E Sbjct: 367 LFMPK-DKAMALINTSESQKIADEVADKAVTLVKDKQKDIFPVNPERYRHILLVNVEGYK 425 Query: 422 NALG--YSKTNGLAAAFIP-LLENEGFKVTRFEP-----------------AGVYEGLQS 461 G + A+ ++ LLE G +VT +E A VY Q Sbjct: 426 GGFGAMIAGNKQRASDYMKELLEARGHEVTVWESTEERIMKLPQEERAAAIANVYAQKQ- 484 Query: 462 SFTEMKESYDLILYLCNLATKSNQTTVRIEWLNPMGL-NVPNYIHSIPTLFISTENPYHL 520 + + YDLI+ L ++ + TT RI W G + P Y+H IP++ IS ++ + L Sbjct: 485 PIANLTDRYDLIINLVDV--NAGGTTQRIIWPAAKGTPDQPFYVHEIPSIVISVQHAFAL 542 Query: 521 LDVPRVKTYINTYGVNDYTLPQLLAKLMGRSQFTGKSPIDPFCGKWDTRL 570 D+P+V TYIN Y T+ ++AKL G S+FTG SP+D +CG DT L Sbjct: 543 ADMPQVGTYINAYDGLPSTISAVVAKLAGESEFTGVSPVDAYCGLIDTHL 592 >ref|NP_687720.1| glycosyl hydrolase, family 3 [Streptococcus agalactiae 2603V/R] gb|AAM99592.1| glycosyl hydrolase, family 3 [Streptococcus agalactiae 2603V/R] Length = 596 Score = 395 bits (1014), Expect = e-108, Method: Compositional matrix adjust. Identities = 220/590 (37%), Positives = 331/590 (56%), Gaps = 32/590 (5%) Query: 9 LREKPFYLSEEDVKWVRAAFGQMTVQEKIGQLFFMVGYKQDEPFLKHIAQEIGIGGLMCR 68 L +KPF L++E ++W+ +MT+ EKIGQLFF +G + E +L + I + Sbjct: 7 LTKKPFNLNQEAIEWIEKTINEMTLDEKIGQLFFNMGASRSEEYLTDVLDRYHIAAVRYN 66 Query: 69 SMAKEEVVATVATLQRSARIPLLIAANLEAGGQGITRDGTKIGSNMAIAATGSSDLAYQL 128 + E+ LQ +++P+LIAAN EAGG G DGTK+G + +AAT AY++ Sbjct: 67 RGSSSEIYDQNLILQTKSKLPMLIAANTEAGGDGAVTDGTKVGDEIKVAATNDPKYAYEM 126 Query: 129 GKICAVEGGSVGANYAFAPVVDIDYNFRNPITNTRTFGSSPQVVMEMGAAYVKGCQEQGM 188 G+I +E +VG N +F+P+VD+ N+RNPI +R +G++ ++ + Y+KG + + Sbjct: 127 GRIAGMEASAVGCNASFSPIVDLTRNWRNPIIASRNWGANVDQIISLSKEYMKGIMQYNI 186 Query: 189 AASVKHFPGDGVDERDQHLVTSVNSLTPEAWDDTYGMVYRHLIEEGAKTVMVGHIALPEY 248 KHFPGDG+DERD HL + N ++ E W T+G +Y L + G VM GHI LP Sbjct: 187 VPFAKHFPGDGIDERDHHLSFASNPMSKEEWMSTFGRIYGELADAGLPGVMAGHIHLPNV 246 Query: 249 SKLLNPSLRDEEILPASLASXXXXXXXXXXXXFNGLIITDATTMAGMNIPLPRNELVPRA 308 K ++P +++LPASL +NG I+TDA+ M GM + R +L+P A Sbjct: 247 EKEMHPERDLDDMLPASLNKTLLDELLRGELGYNGAIVTDASHMVGMTASMARRDLLPTA 306 Query: 309 IENGCDMFLFTKNLEEDVRFMKEGLEAGWLSPGRLDDAVLRILGLKASLKLHEREN---- 364 IE GCD+FLF + +ED+++MKEG E G L+ RL DA+ R LGLKA L LH E Sbjct: 307 IEAGCDLFLFFNDPDEDIQWMKEGYEKGILTEERLHDALRRTLGLKAKLGLHNYEGRRQE 366 Query: 365 --VPVLAESSAVLRQDSHLEIARAVADQSITLVKE-EAGVLPLSPGRYPRVLLYDIESEA 421 +P ++ A++ +IA VAD+++TLVK+ + + P++P RY +LL ++E Sbjct: 367 LFMPK-DKAMALINTLESQKIADEVADKAVTLVKDKQKDIFPVNPERYRHILLVNVEGYK 425 Query: 422 NALG--YSKTNGLAAAFIP-LLENEGFKVTRFEP-----------------AGVYEGLQS 461 G + A+ ++ LLE G +VT +E A VY Q Sbjct: 426 GGFGAMIAGNKQRASDYMKELLEARGHEVTVWESTEERIMKLPQEERAAAIANVYAQKQ- 484 Query: 462 SFTEMKESYDLILYLCNLATKSNQTTVRIEWLNPMGL-NVPNYIHSIPTLFISTENPYHL 520 + E YDLI+ L ++ + TT RI W G + P Y+H IP++ IS ++ + L Sbjct: 485 PIANLTEHYDLIINLVDV--NAGGTTQRIIWPAAKGTPDQPFYVHEIPSIVISVQHAFAL 542 Query: 521 LDVPRVKTYINTYGVNDYTLPQLLAKLMGRSQFTGKSPIDPFCGKWDTRL 570 D+P+V TYIN Y T+ ++AKL G S+FTG SP+D +CG DT L Sbjct: 543 ADMPQVGTYINAYDGLPSTISAVVAKLAGESEFTGVSPVDAYCGLIDTHL 592 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.136 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 833,123,126 Number of extensions: 34487310 Number of successful extensions: 84976 Number of sequences better than 1.0e-05: 379 Number of HSP's gapped: 84606 Number of HSP's successfully gapped: 379 Length of query: 570 Length of database: 757,237,578 Length adjustment: 131 Effective length of query: 439 Effective length of database: 401,559,609 Effective search space: 176284668351 Effective search space used: 176284668351 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)