BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0260.0030.p (386 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00033116.1| COG2814: Arabinose efflux permease [Burkholde... 334 2e-90 gb|EAJ36315.1| unknown [environmental sequence] 334 3e-90 ref|NP_825457.1| putative transmembrane efflux protein [Streptom... 326 5e-88 gb|EAK32413.1| unknown [environmental sequence] 323 3e-87 ref|ZP_00083473.1| COG2814: Arabinose efflux permease [Pseudomon... 321 2e-86 >ref|ZP_00033116.1| COG2814: Arabinose efflux permease [Burkholderia fungorum] Length = 388 Score = 334 bits (857), Expect = 2e-90, Method: Compositional matrix adjust. Identities = 172/340 (50%), Positives = 241/340 (70%) Query: 1 LGAFAIGMTEFIIMGLLPNVARDLGVSIPQAGQLITSYALGVAIGAPVLTIFTHRLPQKK 60 + AFAIG TEF+IMGLLP VA DL VSIP AG L++ YALGVA+GAP+L + T ++P+K Sbjct: 10 ISAFAIGTTEFVIMGLLPEVAHDLAVSIPSAGLLVSGYALGVAVGAPLLAVVTSKMPRKL 69 Query: 61 LLMILMCIFIFGNAVSVIAPTYTLLILARILTAFAHGTFLGVGTIIAARLVRPEKRAGAV 120 L +LM +FI GN + IA Y++L++AR++T+FAHG+F G+G ++AA LV EKRA A+ Sbjct: 70 ALQLLMGVFIVGNTLCAIASGYSVLMIARVVTSFAHGSFFGIGAVVAASLVPAEKRASAI 129 Query: 121 SVVLAGLTVANIIGVPFGTFIGQQLGWRASFGAITVLGVVSLFGIIRFIPVIVQEQTANL 180 +++ GLT+AN++GVPFGTFIGQ+ GWRA+F ++ GV+SL GI +P A L Sbjct: 130 ALMFTGLTLANVLGVPFGTFIGQEFGWRAAFWIVSGFGVLSLAGITALLPNRHDAGPAGL 189 Query: 181 SEQVRGLLNPKVLLILLTGALGCASLFSLFTYITPMLEEITGFAEHNVTWILVLFGAGVT 240 +VR L +P+V L LG +F +FTYI P+LE+++GF+ VT ILVLFG G+T Sbjct: 190 GHEVRVLKDPQVWTALAMTVLGFGGVFVVFTYIAPILEQVSGFSPRGVTLILVLFGVGLT 249 Query: 241 LGNLIGGKLADWKLMPSLIVNFAILAIMISLFSWTLHNPVLTIINVFMWGIAAFGIMPGI 300 +GN +GGKLAD LMPSL+ LA+++++F+ T H+ V I +F+WGIAAF +P + Sbjct: 250 IGNTVGGKLADRALMPSLMGILVALAVVMAIFARTSHSQVAAAITIFVWGIAAFATVPPL 309 Query: 301 QLRIMNLAHKAPLLATTSSHSALNLGNATGAYLGGAAITQ 340 Q+R++ A AP LA+T + A N+GNA GA+LGG I Sbjct: 310 QMRVVEKAAAAPNLASTLNIGAFNVGNAGGAWLGGLVINH 349 >gb|EAJ36315.1| unknown [environmental sequence] Length = 415 Score = 334 bits (856), Expect = 3e-90, Method: Compositional matrix adjust. Identities = 174/341 (51%), Positives = 241/341 (70%), Gaps = 2/341 (0%) Query: 1 LGAFAIGMTEFIIMGLLPNVARDLGVSIPQAGQLITSYALGVAIGAPVLTIFTHRLPQKK 60 + AFAIG TEF+IMGLLP VARDL VS+P AG L+T YALGVA GAP+L + T R+P+K Sbjct: 34 ISAFAIGTTEFVIMGLLPEVARDLAVSLPSAGLLVTGYALGVAAGAPLLAVLTSRMPRKA 93 Query: 61 LLMILMCIFIFGNAVSVIAPTYTLLILARILTAFAHGTFLGVGTIIAARLVRPEKRAGAV 120 L +LM IFI GN + A Y +L++AR++T+FAHG+F G+G ++AA LV +KRA A+ Sbjct: 94 ALQLLMAIFIVGNVLCATASGYAMLMVARVVTSFAHGSFFGIGAVVAASLVPADKRASAI 153 Query: 121 SVVLAGLTVANIIGVPFGTFIGQQLGWRASFGAITVLGVVSLFGIIRFIPVIVQEQTANL 180 +++ GLT++N++GVPFGTF+GQ LGWRASF + LGV+SL G+ +P L Sbjct: 154 ALMFTGLTLSNVLGVPFGTFVGQLLGWRASFWIVAALGVLSLGGVAALVPNRHDSGPVGL 213 Query: 181 SEQVRGLLNPKVLLILLTGALGCASLFSLFTYITPMLEEITGFAEHNVTWILVLFGAGVT 240 +VR L P+V L LL LG +F +FTYI P+L+ ++G++ +V ILVLFGAG+T Sbjct: 214 GHEVRVLKEPQVWLALLMTVLGFGGVFVVFTYIAPILQSVSGYSPRSVALILVLFGAGLT 273 Query: 241 LGNLIGGKLADWKLMPSLI-VNFAILAIMISLFSWTLHNPVLTIINVFMWGIAAFGIMPG 299 +GN +GGKLAD LMPSLI + A++A+M+ +F+ T H+PV + VF+WGIAAF +P Sbjct: 274 IGNTLGGKLADRALMPSLIAILVALMAVMV-VFAKTSHHPVAAAVTVFVWGIAAFATVPP 332 Query: 300 IQLRIMNLAHKAPLLATTSSHSALNLGNATGAYLGGAAITQ 340 +Q ++ A AP LA+T + A N+GNA GA+LGG A+ Sbjct: 333 LQAHVVEKAASAPHLASTLNIGAFNVGNALGAWLGGLALEH 373 >ref|NP_825457.1| putative transmembrane efflux protein [Streptomyces avermitilis MA-4680] dbj|BAC71992.1| putative transmembrane efflux protein [Streptomyces avermitilis MA-4680] Length = 402 Score = 326 bits (836), Expect = 5e-88, Method: Compositional matrix adjust. Identities = 160/338 (47%), Positives = 231/338 (68%) Query: 1 LGAFAIGMTEFIIMGLLPNVARDLGVSIPQAGQLITSYALGVAIGAPVLTIFTHRLPQKK 60 +GAF IG TEF+IMG+LP VA D GVSIP AG L+T YALGV GAP++T ++P+K+ Sbjct: 10 IGAFGIGTTEFVIMGVLPEVAGDFGVSIPTAGLLVTGYALGVVFGAPIMTALGTKVPRKR 69 Query: 61 LLMILMCIFIFGNAVSVIAPTYTLLILARILTAFAHGTFLGVGTIIAARLVRPEKRAGAV 120 +LM+LM +F+ GN +S +AP + L+++ R++ + AHG F G+G+++AA LV PEK+AGA+ Sbjct: 70 MLMLLMGLFVLGNLLSAVAPVFGLMLVGRVVASLAHGAFFGIGSVVAAELVAPEKKAGAI 129 Query: 121 SVVLAGLTVANIIGVPFGTFIGQQLGWRASFGAITVLGVVSLFGIIRFIPVIVQEQTANL 180 S++ +GLTVAN++GVP GT IGQ +GWR +F + LGVV L G+ + +P + + L Sbjct: 130 SMMFSGLTVANVVGVPLGTLIGQSIGWRVTFAVVAALGVVGLLGVAKLVPEMPKPPGVRL 189 Query: 181 SEQVRGLLNPKVLLILLTGALGCASLFSLFTYITPMLEEITGFAEHNVTWILVLFGAGVT 240 ++ N +VLL + LG +F+ TYI PM+ + GFA+ +VTW+LVLFG G+ Sbjct: 190 RHELAAFKNVQVLLAMAMTVLGFGGVFAAITYIAPMMTHVAGFADGSVTWLLVLFGLGMV 249 Query: 241 LGNLIGGKLADWKLMPSLIVNFAILAIMISLFSWTLHNPVLTIINVFMWGIAAFGIMPGI 300 GNLIGGK AD LMP L V+ LA++++LF+ T HN VL + + + G F +P + Sbjct: 250 GGNLIGGKFADRALMPMLYVSLGGLAVVLALFTVTAHNKVLAAVTIMLIGALGFATVPPL 309 Query: 301 QLRIMNLAHKAPLLATTSSHSALNLGNATGAYLGGAAI 338 Q R+++ AH AP LA+ + A NLGNA A+LGG I Sbjct: 310 QKRVLDHAHGAPTLASAVNIGAFNLGNALSAWLGGIVI 347 >gb|EAK32413.1| unknown [environmental sequence] Length = 389 Score = 323 bits (829), Expect = 3e-87, Method: Compositional matrix adjust. Identities = 169/338 (50%), Positives = 246/338 (72%) Query: 1 LGAFAIGMTEFIIMGLLPNVARDLGVSIPQAGQLITSYALGVAIGAPVLTIFTHRLPQKK 60 + AF IG TEF+IMGLLP+VARDLGVSIP AG L++ YALGV IGAP+L I T ++P+K+ Sbjct: 10 VAAFGIGTTEFVIMGLLPDVARDLGVSIPAAGMLVSGYALGVTIGAPILAIVTAKMPRKR 69 Query: 61 LLMILMCIFIFGNAVSVIAPTYTLLILARILTAFAHGTFLGVGTIIAARLVRPEKRAGAV 120 LM L+ +FI GN IAP Y +L+ AR++TAF HG F G+G+++A+ LV P +RA A+ Sbjct: 70 ALMGLIGLFIAGNLFCAIAPGYAVLMAARVVTAFCHGAFFGIGSVVASNLVAPNRRAQAI 129 Query: 121 SVVLAGLTVANIIGVPFGTFIGQQLGWRASFGAITVLGVVSLFGIIRFIPVIVQEQTANL 180 +++ GLT+AN++GVP GT +GQ GWRA+F A+T +G+ + + +P + ++ Sbjct: 130 ALMFTGLTLANVLGVPLGTALGQAYGWRATFWAVTGIGIAAAAALAVCLPKNLAMPDTSI 189 Query: 181 SEQVRGLLNPKVLLILLTGALGCASLFSLFTYITPMLEEITGFAEHNVTWILVLFGAGVT 240 + + L +P+VL++L L ASLFS+FTYITP+LE++TGF+ VT++L+LFG G+T Sbjct: 190 TREFSVLKHPQVLMVLGISVLASASLFSVFTYITPILEDVTGFSPRQVTYVLLLFGLGLT 249 Query: 241 LGNLIGGKLADWKLMPSLIVNFAILAIMISLFSWTLHNPVLTIINVFMWGIAAFGIMPGI 300 +G +GGKLADW+ MPSLI A++ ++++LF+ T+H P + +F+WGI AF I+P + Sbjct: 250 VGGTLGGKLADWRRMPSLIATLALIGVVLALFAGTMHLPFAALATIFVWGILAFAIVPPL 309 Query: 301 QLRIMNLAHKAPLLATTSSHSALNLGNATGAYLGGAAI 338 Q+ I++ A AP LA+T + A NLGNATGA+LGG AI Sbjct: 310 QILIVDRASDAPNLASTLNQGAFNLGNATGAWLGGMAI 347 >ref|ZP_00083473.1| COG2814: Arabinose efflux permease [Pseudomonas fluorescens PfO-1] Length = 387 Score = 321 bits (822), Expect = 2e-86, Method: Compositional matrix adjust. Identities = 166/345 (48%), Positives = 238/345 (68%), Gaps = 1/345 (0%) Query: 4 FAIGMTEFIIMGLLPNVARDLGVSIPQAGQLITSYALGVAIGAPVLTIFTHRLPQKKLLM 63 FAIG TEF+IMGLLPNVA DLGVSIP AG L+T YALGVAIGAP + + T +LP+K L+ Sbjct: 13 FAIGTTEFVIMGLLPNVAADLGVSIPGAGWLVTGYALGVAIGAPFMAMATAKLPRKAALV 72 Query: 64 ILMCIFIFGNAVSVIAPTYTLLILARILTAFAHGTFLGVGTIIAARLVRPEKRAGAVSVV 123 LM IFI GN + IA Y +L+ AR++TA HG F G+G+++AA LV P KRA AV+++ Sbjct: 73 TLMGIFIIGNLLCAIATDYNVLMFARVVTALCHGAFFGIGSVVAANLVAPNKRASAVALM 132 Query: 124 LAGLTVANIIGVPFGTFIGQQLGWRASFGAITVLGVVSLFGIIRFIPVIVQEQTANLSEQ 183 GLT+AN++GVP GT +GQ+ GWR++F A+TV+GV++L G+IRF+P E+ ++ + Sbjct: 133 FTGLTLANVLGVPLGTALGQEAGWRSTFWAVTVIGVIALIGLIRFLPAKRDEEKLDMRAE 192 Query: 184 VRGLLNPKVLLILLTGALGCASLFSLFTYITPMLEEITGFAEHNVTWILVLFGAGVTLGN 243 + L + L L AL AS+F+LFTY+ P+L ++TG + VTW L+L G G+T+GN Sbjct: 193 LAALKGAGIWLSLSMTALFAASVFTLFTYVAPLLGDVTGVSPRGVTWTLMLIGLGLTVGN 252 Query: 244 LIGGKLADWKLMPSLIVNFAILAIMISLFSWTLHNPVLTIINVFMWGIAAFGIMPGIQLR 303 +IGGKLAD L +LI F +A+ ++ +WT + T I +F+W A F +P +Q+ Sbjct: 253 IIGGKLADKGLAATLIGVFIAMAVTSTVLTWTSIALIPTEITLFLWATACFAAVPALQVN 312 Query: 304 IMNLAHKAPLLATTSSHSALNLGNATGAYLGGAAITQ-WGLSSIP 347 ++ AP L +T + A N+GNA GA++GG+ I +GL+S+P Sbjct: 313 VVTYGQAAPNLVSTLNIGAFNVGNALGAWVGGSVIAHGYGLTSVP 357 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.327 0.141 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 500,600,101 Number of extensions: 20115258 Number of successful extensions: 82416 Number of sequences better than 1.0e-05: 896 Number of HSP's gapped: 82483 Number of HSP's successfully gapped: 897 Length of query: 386 Length of database: 757,237,578 Length adjustment: 127 Effective length of query: 259 Effective length of database: 412,420,005 Effective search space: 106816781295 Effective search space used: 106816781295 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 127 (53.5 bits)