BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0260.0023.p (311 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00186137.1| hypothetical protein Rxyl306701 [Rubrobacter ... 237 2e-61 ref|NP_242866.1| 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase [... 226 4e-58 ref|NP_869236.1| 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-... 214 2e-54 gb|EAK21152.1| unknown [environmental sequence] 211 1e-53 ref|NP_991359.1| CGI-105 protein [Bos taurus] >gi|37780029|gb|AA... 209 6e-53 >ref|ZP_00186137.1| hypothetical protein Rxyl306701 [Rubrobacter xylanophilus DSM 9941] Length = 270 Score = 237 bits (605), Expect = 2e-61, Method: Compositional matrix adjust. Identities = 123/216 (56%), Positives = 154/216 (71%), Gaps = 10/216 (4%) Query: 90 DPGKIICIGLNYRRHAEETGMPLPEYPILFSKFSNSLSGHGMEVPLPYSTQKVDYEAELG 149 +P KII IGLNY HA ETG P+PE PI+F+K++N+L G G EV +P T + DYEAEL Sbjct: 59 NPQKIIAIGLNYEDHANETGAPIPEKPIVFAKYANTLVGAGEEVRIPPITSQADYEAELA 118 Query: 150 IMIGRTAKNVSEEEALDAVFGYFAANDLSARDLQ-TRTSQWLGGEXXXXAGKSCDRFAPV 208 ++IGR AKNVSEEEAL+ VFGY ND+S+RDLQ + QW KS D F P+ Sbjct: 119 VVIGREAKNVSEEEALEYVFGYTNCNDVSSRDLQFSEGGQW-------TRSKSLDTFCPL 171 Query: 209 GPYVVTEDEVGDPGLLRISCSVNGEVRQDSNTSDMIFDCRQIISYISSCMTLSPGDIILT 268 GPYV T DE+ DP LRI +NGEV QDSNTS MIF ++IS++S+ MTL PGDII T Sbjct: 172 GPYVATRDEIPDPQSLRIRSVLNGEVMQDSNTSKMIFPVARLISFLSTGMTLVPGDIIAT 231 Query: 269 GTPEGVMMGYPPEKQVYLQPGDEVTVEIEKLGRLTN 304 GTP GV G+ + +V+L+ GDE+++EIE LGRLTN Sbjct: 232 GTPAGV--GFARDPKVFLKDGDEISIEIEGLGRLTN 265 >ref|NP_242866.1| 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase [Bacillus halodurans] pir||H83899 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase BH2000 [imported] - Bacillus halodurans (strain C-125) dbj|BAB05719.1| 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase [Bacillus halodurans] Length = 319 Score = 226 bits (577), Expect = 4e-58, Method: Compositional matrix adjust. Identities = 126/318 (39%), Positives = 187/318 (58%), Gaps = 23/318 (7%) Query: 1 MKLMNFWESGNLRLGVKKEEGILDVXXX---------XXXXXXXXXXXVPATLREVLDGG 51 MK++N+ ++R+G ++D+ VPA L+GG Sbjct: 1 MKIINYRSKDHVRIGCVIGNYVIDLNRAYAAMLEAQGLAKANEIADAVVPANSLSFLEGG 60 Query: 52 EAALDSLRKFADSLPYHD---EGAKAFYRDETLLEFAPCTADPGKIICIGLNYRRHAEET 108 E K S+ ++ + +K F+ E E AP + KIIC+G NYR H E Sbjct: 61 EHTFLEASKAVQSIQKNELTPDQSKVFHEREVKKE-APIL-NSKKIICVGHNYREHILEM 118 Query: 109 GMPLPEYPILFSKFSNSLSGHGMEVPLPYSTQKVDYEAELGIMIGRTAKNVSEEEALDAV 168 +P+YP++F+KF+N++ G VPLP T+++DYEAEL +IG+ AK+V++E+ALD V Sbjct: 119 KREIPKYPVIFAKFANTIIGPEDNVPLPSITEQLDYEAELAFVIGKKAKDVAKEDALDYV 178 Query: 169 FGYFAANDLSARDLQTRTSQWLGGEXXXXAGKSCDRFAPVGPYVVTEDEVGDPGLLRISC 228 GY ND++ARDLQ RT QWL GK+ D P+GP++VT+DE+ +P L I+ Sbjct: 179 AGYTIVNDITARDLQRRTIQWL-------QGKTLDGSLPMGPWLVTKDEIPNPHQLEITL 231 Query: 229 SVNGEVRQDSNTSDMIFDCRQIISYISSCMTLSPGDIILTGTPEGVMMGYPPEKQVYLQP 288 SVNGE RQ SNT +++F + ++ ++S MTL PGD++ TGTP GV G E Q++LQ Sbjct: 232 SVNGEERQRSNTKNLVFSIQDLVVFLSGIMTLEPGDVVCTGTPGGV--GVAREPQLFLQD 289 Query: 289 GDEVTVEIEKLGRLTNRM 306 GD V +E+EK+G L NR+ Sbjct: 290 GDVVRIELEKVGTLENRV 307 >ref|NP_869236.1| 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase [Pirellula sp. 1] emb|CAD76622.1| 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase [Pirellula sp.] Length = 342 Score = 214 bits (545), Expect = 2e-54, Method: Compositional matrix adjust. Identities = 119/249 (47%), Positives = 151/249 (60%), Gaps = 15/249 (6%) Query: 63 DSLPYHDEGAKAFYRDETLLEFAPCTADPGKIICIGLNYRRHAEETGMPLPEYPILFSKF 122 D LP E D+ L P P KI+CIGLNY HA ETG P P++FSKF Sbjct: 106 DQLPTPTEAQWIDAPDQLL----PPVTCPEKILCIGLNYLDHAIETGAEKPSLPVVFSKF 161 Query: 123 SNSLSGHGMEVPLPYSTQKVDYEAELGIMIGRTAKNVSEEEALDAVFGYFAANDLSARDL 182 +++L GHG ++ LP + KVDYEAEL ++IG+T ++V EA+DAVFGY +D+S+RD Sbjct: 162 NSALVGHGQDIVLPKISDKVDYEAELVVVIGKTVRHVDANEAMDAVFGYAVGHDVSSRDW 221 Query: 183 QTRT--SQWLGGEXXXXAGKSCDRFAPVGPYVVTEDEVGDPGLLRISCSVNGEVRQDSNT 240 Q QWL GKS D FAP+GP VVT DE+ DPG L I +NGE Q+S T Sbjct: 222 QKGRPGGQWL-------VGKSFDTFAPLGPAVVTADEISDPGNLPIRLHINGETLQESKT 274 Query: 241 SDMIFDCRQIISYISSCMTLSPGDIILTGTPEGVMMGYPPEKQVYLQPGDEVTVEIEKLG 300 +IFD +I+++S MTL PGD+I TGTP GV P + YL PGD VEI+ +G Sbjct: 275 DQLIFDIPALIAHLSKFMTLKPGDLIFTGTPSGVGDARTPPR--YLAPGDRCVVEIDGIG 332 Query: 301 RLTNRMVAE 309 RL N AE Sbjct: 333 RLENTCQAE 341 >gb|EAK21152.1| unknown [environmental sequence] Length = 282 Score = 211 bits (538), Expect = 1e-53, Method: Compositional matrix adjust. Identities = 117/258 (45%), Positives = 160/258 (62%), Gaps = 23/258 (8%) Query: 55 LDSLRKFAD-SLPYHDEGAKAFYRDETLLEFAPCTADP--GKIICIGLNYRRHAEETGMP 111 LD ++KF + +LP ET +APC GK ICIGLNY HAEETG Sbjct: 43 LDEVKKFDNITLPLV----------ETHDGYAPCVCKKSVGKFICIGLNYSDHAEETGQK 92 Query: 112 LPEYPILFSKFSNSLSGHGMEVPLPYSTQKVDYEAELGIMIGRTAKNVSEEEALDAVFGY 171 +P+ PI+FSK ++++ G +V +P ++ K D+E ELGI+IG+ AK +SE ++ + GY Sbjct: 93 VPKEPIIFSKATSAIIGPNDDVEIPKNSTKSDWEVELGIIIGKEAKYISESDSQSHIAGY 152 Query: 172 FAANDLSARDLQTRTS-QWLGGEXXXXAGKSCDRFAPVGPYVVTEDEVGDPGLLRISCSV 230 NDLS R+ Q S QW+ GKSCD F P+GPY+VT DEV DP L++ V Sbjct: 153 CVINDLSEREFQIEHSGQWV-------KGKSCDTFGPIGPYIVTTDEVPDPQNLKMWLDV 205 Query: 231 NGEVRQDSNTSDMIFDCRQIISYISSCMTLSPGDIILTGTPEGVMMGYPPEKQVYLQPGD 290 NG+ Q+ +TS M+F I+SY+S M+L PGDII TGTP GV MG P QV+L+PGD Sbjct: 206 NGKRMQNGSTSTMVFGVNFIVSYLSQFMSLQPGDIISTGTPPGVGMGMKP--QVFLKPGD 263 Query: 291 EVTVEIEKLGRLTNRMVA 308 E+ + IE LG T + ++ Sbjct: 264 EIKLGIEGLGEQTQKTIS 281 >ref|NP_991359.1| CGI-105 protein [Bos taurus] gb|AAP33277.1| CGI-105 protein [Bos taurus] Length = 314 Score = 209 bits (532), Expect = 6e-53, Method: Compositional matrix adjust. Identities = 116/272 (42%), Positives = 165/272 (60%), Gaps = 23/272 (8%) Query: 40 VPATLREVLDGGEAALDSLRK-FADSLPYHDEGAKAFYRDETLLEFAPCTADPGKIICIG 98 +P T+ E L+ GEA L +R+ A LP F AP T P K++C+G Sbjct: 62 LPKTMVEFLEQGEATLSVVRRALATQLPVLPRSEVTF--------LAPVT-RPDKVVCVG 112 Query: 99 LNYRRHAEETGMPLPEYPILFSKFSNSLSGHGMEVPLPYSTQKVDYEAELGIMIGRTAKN 158 +NY H E +P+P+ PI+FSKF++++ G + LP +QKVD+E EL ++IG+ K Sbjct: 113 MNYADHCREQNVPVPKEPIIFSKFASAIVGPYDNIILPPESQKVDWEVELAVVIGKRGKY 172 Query: 159 VSEEEALDAVFGYFAANDLSARDLQT--RTSQWLGGEXXXXAGKSCDRFAPVGPYVVTED 216 + +A+ V G+ A+D+SARD Q QWL GK+ D F P+GP +VT+D Sbjct: 173 IKATDAMAHVAGFTVAHDVSARDWQMGRNGKQWL-------LGKTFDTFCPLGPALVTKD 225 Query: 217 EVGDPGLLRISCSVNGEVRQDSNTSDMIFDCRQIISYISSCMTLSPGDIILTGTPEGV-M 275 V DP L+I C VNGEV Q SNT+ M+F ++I+++S +TL PGDIILTGTP GV + Sbjct: 226 SVADPHNLKICCRVNGEVMQSSNTNQMVFKTEELITWVSQFVTLYPGDIILTGTPPGVGV 285 Query: 276 MGYPPEKQVYLQPGDEVTVEIEKLGRLTNRMV 307 PP V+L+ GDEV EIE+LG + N++V Sbjct: 286 FRKPP---VFLKKGDEVQCEIEELGVIINKVV 314 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.317 0.136 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 460,126,898 Number of extensions: 18930861 Number of successful extensions: 38255 Number of sequences better than 1.0e-05: 874 Number of HSP's gapped: 36784 Number of HSP's successfully gapped: 883 Length of query: 311 Length of database: 757,237,578 Length adjustment: 125 Effective length of query: 186 Effective length of database: 417,850,203 Effective search space: 77720137758 Effective search space used: 77720137758 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 126 (53.1 bits)