BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0260.0022.p (207 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_978918.1| regucalcin family protein [Bacillus cereus ATCC... 196 2e-49 ref|NP_832293.1| hypothetical Membrane Associated Protein [Bacil... 193 3e-48 ref|NP_344027.1| Regucalcin homolog [Sulfolobus solfataricus] >g... 189 4e-47 sp|Q9TTJ6|SM30_RABIT Senescence marker protein-30 (SMP-30) (Regu... 186 2e-46 ref|NP_004674.1| regucalcin; senescence marker protein-30 [Homo ... 180 2e-44 >ref|NP_978918.1| regucalcin family protein [Bacillus cereus ATCC 10987] gb|AAS41526.1| regucalcin family protein [Bacillus cereus ATCC 10987] Length = 300 Score = 196 bits (499), Expect = 2e-49, Method: Compositional matrix adjust. Identities = 95/207 (45%), Positives = 125/207 (60%), Gaps = 3/207 (1%) Query: 2 ELLAHV---EKDLPGNRLNDAKCDSKGRLWFGTMSMEDKRDAGSLYVMEPGGEVRKVLSG 58 E L H+ E L NR ND KCD GR W GT AGSLY + +V K +S Sbjct: 84 EKLTHIFDPEPHLFDNRFNDGKCDPAGRFWAGTTDTYGINAAGSLYCLNNSFKVEKKVSH 143 Query: 59 IGISNGLAWDDSCGLMYYIDTLTRGVDVMDYDLQNGTVSGRRTVVRFPEGAGSPDGMTID 118 + SNG+AW +YYIDT T+ V Y++ G + V+ FPE G PDGMTID Sbjct: 144 VNTSNGIAWSPDYTYLYYIDTPTKKVVRFHYNICTGEIRNPNDVIIFPENDGLPDGMTID 203 Query: 119 SEGMIWVAHWNGGCVSRWNPHTGEQIGKIEVPAPFVTSCAFGGGHLNELYITSARGSMSP 178 EG +W+AHW G ++RWNP TGE+I I +PA +VTSC FGG +L +LY+T+AR M+ Sbjct: 204 KEGCLWIAHWGGSKITRWNPSTGERILSIPIPALYVTSCTFGGPNLTDLYVTTARTRMTN 263 Query: 179 EELQRWPLAGGLFKANTGIRGVPANCF 205 EL+ +P AGG+F+ T I+G P + F Sbjct: 264 NELKEYPHAGGIFRIQTNIKGCPTHSF 290 >ref|NP_832293.1| hypothetical Membrane Associated Protein [Bacillus cereus ATCC 14579] gb|AAP09494.1| hypothetical Membrane Associated Protein [Bacillus cereus ATCC 14579] Length = 300 Score = 193 bits (490), Expect = 3e-48, Method: Compositional matrix adjust. Identities = 93/209 (44%), Positives = 122/209 (58%), Gaps = 3/209 (1%) Query: 2 ELLAHV---EKDLPGNRLNDAKCDSKGRLWFGTMSMEDKRDAGSLYVMEPGGEVRKVLSG 58 E L H+ E L NR ND KCD GR W G+ AGSLY + V K +S Sbjct: 84 EKLTHIFDPEPHLIENRFNDGKCDPAGRFWAGSTDTYGMNAAGSLYCLHHNLSVEKKVSH 143 Query: 59 IGISNGLAWDDSCGLMYYIDTLTRGVDVMDYDLQNGTVSGRRTVVRFPEGAGSPDGMTID 118 + SNG+AW Y+IDT T+ V Y+++ G + V+ F E G PDGMTID Sbjct: 144 VNTSNGIAWSPDHTYFYFIDTPTKKVVRYQYNIRTGDIHNPSDVINFSENDGLPDGMTID 203 Query: 119 SEGMIWVAHWNGGCVSRWNPHTGEQIGKIEVPAPFVTSCAFGGGHLNELYITSARGSMSP 178 EG +W+AHW G ++RWNP TGEQI I +PA +VTSC FGG +L +LY+T+AR MS Sbjct: 204 EEGCLWIAHWGGSKITRWNPFTGEQILSIPIPALYVTSCTFGGPNLTDLYVTTARTRMSD 263 Query: 179 EELQRWPLAGGLFKANTGIRGVPANCFAG 207 EL+ +P AGG+F+ T ++G P F Sbjct: 264 NELKEYPHAGGIFRIQTNVKGCPTYSFCN 292 >ref|NP_344027.1| Regucalcin homolog [Sulfolobus solfataricus] pir||B90445 regucalcin homolog [imported] - Sulfolobus solfataricus gb|AAK42817.1| Regucalcin homolog [Sulfolobus solfataricus] Length = 285 Score = 189 bits (479), Expect = 4e-47, Method: Compositional matrix adjust. Identities = 91/200 (45%), Positives = 124/200 (62%), Gaps = 8/200 (4%) Query: 1 LELLAHVEKDLPGNRLNDAKCDSKGRLWFGTMSMEDKRDAGSLYVMEPGGEVRKVLSGIG 60 ++ +A VE D+ NR ND KCD GR W GTM+M +++ GS Y ++ G ++ K+L G+ Sbjct: 86 IQKIAEVETDMESNRFNDGKCDKLGRYWAGTMNMNERKPTGSFYKLDTG-KITKILEGLT 144 Query: 61 ISNGLAWDDSCGLMYYIDTLTRGVDVMDYDLQNGTVSGRRTVVRFPEGAGSPDGMTIDSE 120 +SNGL WD M+ ID+ R V V D+DL G + RR V F G+PDGM +D E Sbjct: 145 VSNGLGWDPDNTQMFLIDSPVRKVFVFDFDLNKGEIYNRRVAVDFGNEPGNPDGMAVDEE 204 Query: 121 GMIWVAHWNGGCVSRWNPHTGEQIGKIEVPAPFVTSCAFGGGHLNELYITSARGSMSPEE 180 G IWVAHW GG VSRWNP TG+++ +I+VPA +VTS FG L++L+IT+A S Sbjct: 205 GYIWVAHWGGGKVSRWNPKTGKKLFEIKVPATYVTSVTFGTQELDQLFITTAGKSQD--- 261 Query: 181 LQRWPLAGGLFKANTGIRGV 200 PLAG F +RG+ Sbjct: 262 ----PLAGKTFTTKANVRGL 277 >sp|Q9TTJ6|SM30_RABIT Senescence marker protein-30 (SMP-30) (Regucalcin) (RC) dbj|BAA88079.1| regucalcin [Oryctolagus cuniculus] Length = 299 Score = 186 bits (473), Expect = 2e-46, Method: Compositional matrix adjust. Identities = 92/210 (43%), Positives = 127/210 (60%), Gaps = 6/210 (2%) Query: 4 LAHVEKDLPGNRLNDAKCDSKGRLWFGTMSMED-----KRDAGSLYVMEPGGEVRKVLSG 58 LA V+KD NR ND K D GR + GTM+ E +R GSLY + P +V+K Sbjct: 90 LATVDKDKKNNRFNDGKVDPAGRYFAGTMAEETAPAVLERHQGSLYALFPDHQVKKYFDQ 149 Query: 59 IGISNGLAWDDSCGLMYYIDTLTRGVDVMDYDLQNGTVSGRRTVVRFPEGAGSPDGMTID 118 + ISNGL W + YYID+L VD DYDLQ G +S RR++ + + PDGM ID Sbjct: 150 VDISNGLDWSLDHKIFYYIDSLAYSVDAFDYDLQTGQISNRRSIYKLEKEEQIPDGMCID 209 Query: 119 SEGMIWVAHWNGGCVSRWNPHTGEQIGKIEVPAPFVTSCAFGGGHLNELYITSARGSMSP 178 +EG +WVA +NGG V R +P TG+++ +++P TSC FGG +E+Y+T AR + P Sbjct: 210 TEGKLWVACYNGGRVIRLDPETGKRLQTVKLPVDKTTSCCFGGKDYSEMYVTCARDGLDP 269 Query: 179 EELQRWPLAGGLFKAN-TGIRGVPANCFAG 207 + L R P AGG+FK G++G+P +AG Sbjct: 270 DSLSRQPEAGGIFKITGLGVKGIPPYSYAG 299 >ref|NP_004674.1| regucalcin; senescence marker protein-30 [Homo sapiens] ref|NP_690608.1| regucalcin; senescence marker protein-30 [Homo sapiens] sp|Q15493|SM30_HUMAN Senescence marker protein-30 (SMP-30) (Regucalcin) (RC) pir||S60035 senescence marker protein 30 - human dbj|BAA06602.1| SMP-30 [Homo sapiens] dbj|BAA78693.1| regucalcin [Homo sapiens] dbj|BAA84082.1| regucalcin [Homo sapiens] Length = 299 Score = 180 bits (457), Expect = 2e-44, Method: Compositional matrix adjust. Identities = 92/211 (43%), Positives = 127/211 (60%), Gaps = 6/211 (2%) Query: 3 LLAHVEKDLPGNRLNDAKCDSKGRLWFGTMSMED-----KRDAGSLYVMEPGGEVRKVLS 57 +LA V+ D NR ND K D GR + GTM+ E +R G+LY + P V+K Sbjct: 89 VLATVDNDKKNNRFNDGKVDPAGRYFAGTMAEETAPAVLERHQGALYSLFPDHHVKKYFD 148 Query: 58 GIGISNGLAWDDSCGLMYYIDTLTRGVDVMDYDLQNGTVSGRRTVVRFPEGAGSPDGMTI 117 + ISNGL W + YYID+L+ VD DYDLQ G +S RR+V + + PDGM I Sbjct: 149 QVDISNGLDWSLDHKIFYYIDSLSYSVDAFDYDLQTGQISNRRSVYKLEKEEQIPDGMCI 208 Query: 118 DSEGMIWVAHWNGGCVSRWNPHTGEQIGKIEVPAPFVTSCAFGGGHLNELYITSARGSMS 177 D+EG +WVA +NGG V R +P TG+++ +++P TSC FGG + +E+Y+T AR M Sbjct: 209 DAEGKLWVACYNGGRVIRLDPVTGKRLQTVKLPVDKTTSCCFGGKNYSEMYVTCARDGMD 268 Query: 178 PEELQRWPLAGGLFKAN-TGIRGVPANCFAG 207 PE L R P AGG+FK G++G+ +AG Sbjct: 269 PEGLLRQPEAGGIFKITGLGVKGIAPYSYAG 299 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.138 0.444 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 352,870,033 Number of extensions: 15804325 Number of successful extensions: 29162 Number of sequences better than 1.0e-05: 238 Number of HSP's gapped: 28791 Number of HSP's successfully gapped: 238 Length of query: 207 Length of database: 757,237,578 Length adjustment: 120 Effective length of query: 87 Effective length of database: 431,425,698 Effective search space: 37534035726 Effective search space used: 37534035726 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 123 (52.0 bits)