BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0258.0009.p (714 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_901978.1| conserved hypothetical protein [Chromobacterium... 340 5e-92 ref|NP_953557.1| sensory box/GGDEF family protein [Geobacter sul... 340 1e-91 ref|ZP_00014798.1| COG2200: FOG: EAL domain [Rhodospirillum rubrum] 337 7e-91 ref|ZP_00034273.1| COG2199: FOG: GGDEF domain [Burkholderia fung... 334 5e-90 ref|ZP_00082370.1| COG2200: FOG: EAL domain [Geobacter metallire... 332 1e-89 >ref|NP_901978.1| conserved hypothetical protein [Chromobacterium violaceum ATCC 12472] gb|AAQ59980.1| conserved hypothetical protein [Chromobacterium violaceum ATCC 12472] Length = 679 Score = 340 bits (873), Expect = 5e-92, Method: Compositional matrix adjust. Identities = 185/459 (40%), Positives = 265/459 (57%), Gaps = 21/459 (4%) Query: 267 SRFNRELERVVSIRTRDL---------------RVSKRLYEKLAHYDSLTGIPNRLYFMD 311 S NREL+ V+ RT +L R +RL + LAH+D LTG+PNRL F Sbjct: 204 SETNRELDSRVAARTWELANANARLEDEIQERRRAEERL-DHLAHHDPLTGLPNRLLFQK 262 Query: 312 EFERLLQSAE-PTQTYTLFFFDLNRFKEINDTLGHSIGDQVIKTLANRLKNGKVPFKLFA 370 R L + P + F DL+RFK++NDTLGH IGDQ++ +A RL + + A Sbjct: 263 RLSRALSVPDLPPYKLAMLFIDLDRFKDVNDTLGHYIGDQLLNAVAARLADSIRAEDMLA 322 Query: 371 RTGGDEFVMVFSNLAREHIPA-IAEHISGLISETLLISGAHLSLSTSIGVSLYPEHSLNK 429 R GGDEF+ V ++ + A +A + L + I+G L LS SIG+S + Sbjct: 323 RLGGDEFICVLESIENRDMAADVAGKLLALFRQPFCINGNDLYLSASIGISFAGNDGADV 382 Query: 430 DDLLKFADMSMYQAKSQEDANYFIFDWGLREKLEQKTMIAKYLHSALEREEFVLHYQPQI 489 D LL+ AD++MY AK+ + + E EQ+ ++ + L AL+ +E +HYQPQ+ Sbjct: 383 DTLLRHADIAMYSAKNAGRNRCQHYSPQMSEDAEQRVVLERQLRQALDNDEITVHYQPQV 442 Query: 490 NAVTGKMIGMEALVRWNHPEKGLIGPGTFITAVEEAGLMIQLTDWVLREVCRQLCEWRTM 549 +AVTGK++G EAL RW HP G++ P FI EE GL++ L + +L + RQL W Sbjct: 443 DAVTGKLVGAEALARWRHPILGMVPPARFIPVAEECGLIVALGEQILEKSLRQLASWHEA 502 Query: 550 GLPLLRTSINISNSWFYNRNLIENLLAVLDQYGLETSMLEFEITESTALL-EEHYPLLQQ 608 G PL S+N+S ++ +++ +L +Y LE LE EITES + E+ +L + Sbjct: 503 GFPLPSVSVNLSARQLERQDFPQHVARLLKRYRLEPRHLELEITESVIMATEDAVNILAE 562 Query: 609 MRDHGIVVSIDDFGTKYSSLNYLKHFPVNKIKIDRTFITGIGVSSIDETIIKSIVYVASQ 668 +R GI +SIDDFGT YSSL+YL+ PV +K+DR+FI GIG E I+ +I+ ++S Sbjct: 563 LRAQGIRLSIDDFGTGYSSLSYLRQLPVQVLKVDRSFILGIGKGG--EAIVHAIMALSSS 620 Query: 669 LGYDLIAEGVETQEQLDFLIKHNCPHIQGFLFFPPLPAE 707 LG + +AEGVE QL+FL C IQGFL+ PLPAE Sbjct: 621 LGLETVAEGVEEASQLEFLRSAGCHIIQGFLYAAPLPAE 659 >ref|NP_953557.1| sensory box/GGDEF family protein [Geobacter sulfurreducens PCA] gb|AAR35884.1| sensory box/GGDEF family protein [Geobacter sulfurreducens PCA] Length = 873 Score = 340 bits (871), Expect = 1e-91, Method: Compositional matrix adjust. Identities = 171/436 (39%), Positives = 263/436 (60%), Gaps = 7/436 (1%) Query: 283 DLRVSKRLYEKLAH---YDSLTGIPNRLYFMDEFERLLQSAEPT-QTYTLFFFDLNRFKE 338 D+ +R E+L H +D+LTG+PNR D + + A Q L FDL+ FK Sbjct: 422 DITDRRRFQEELLHQATHDTLTGLPNRSLLEDRLNQAVAGARRRGQMVALLMFDLDNFKV 481 Query: 339 INDTLGHSIGDQVIKTLANRLKNGKVPFKLFARTGGDEFVMVFSNLAR-EHIPAIAEHIS 397 +NDTLGH+ GDQ+++++A R+++ FAR GGDEFV+V +NL R + +AE Sbjct: 482 VNDTLGHTAGDQLLRSVAARIRSFVRNCDTFARLGGDEFVIVLTNLTRMDDAVTVAESFR 541 Query: 398 GLISETLLISGAHLSLSTSIGVSLYPEHSLNKDDLLKFADMSMYQAKSQEDANYFIFDWG 457 L++ + G + ++ S+G+SL+P+ + L+K AD +MY K ++ F Sbjct: 542 NLLAMPFFLGGREIFITASMGISLFPDDGDTMEALIKKADTAMYHIKESGRNSFQFFAEE 601 Query: 458 LREKLEQKTMIAKYLHSALEREEFVLHYQPQINAVTGKMIGMEALVRWNHPEKGLIGPGT 517 + +K+ + I L ALE+ E +LHYQP++ TG ++GMEALVRWN P+ GLI P Sbjct: 602 MNQKVNARLTIETGLRRALEKGEMLLHYQPRLEPGTGAVVGMEALVRWNSPDMGLISPAD 661 Query: 518 FITAVEEAGLMIQLTDWVLREVCRQLCEWRTMGLPLLRTSINISNSWFYNRNLIENLLAV 577 FI E++GL++++ DWVL C Q EW MG LR S+NIS F + ++ ++ + Sbjct: 662 FIPIAEDSGLIVEIGDWVLATACCQALEWHRMGHDSLRVSVNISARQFVGHDFVDRVVRI 721 Query: 578 LDQYGLETSMLEFEITES--TALLEEHYPLLQQMRDHGIVVSIDDFGTKYSSLNYLKHFP 635 ++Q GL +E E+TE+ T ++E ++ ++R+ GI +SIDDFGT YSSLNYLK FP Sbjct: 722 IEQSGLSPHFVELELTETALTHNVDETVKIMNRLRERGITISIDDFGTGYSSLNYLKRFP 781 Query: 636 VNKIKIDRTFITGIGVSSIDETIIKSIVYVASQLGYDLIAEGVETQEQLDFLIKHNCPHI 695 V+ +KID++FI + D I+ +I+ +A + ++AEGVET EQ+ L++ NC I Sbjct: 782 VDVLKIDKSFIDDMVKRREDAAIVATIIGIAHHMHMKVVAEGVETAEQMKLLMEGNCEEI 841 Query: 696 QGFLFFPPLPAEDIHK 711 QGF F PLP E+ + Sbjct: 842 QGFYFSRPLPPEEFER 857 >ref|ZP_00014798.1| COG2200: FOG: EAL domain [Rhodospirillum rubrum] Length = 626 Score = 337 bits (864), Expect = 7e-91, Method: Compositional matrix adjust. Identities = 186/450 (41%), Positives = 268/450 (59%), Gaps = 22/450 (4%) Query: 276 VVSIRTR------------DLRVSKRLYEKL---AHYDSLTGIPNRLYFMDEFERLLQSA 320 V ++RTR D+ KR E + A++D LT +PNR MD E+ + A Sbjct: 150 VTAVRTRAGETTHYVAAITDITKRKRDEEHIRFQANFDMLTHLPNRHLIMDRLEQAMHQA 209 Query: 321 EPT-QTYTLFFFDLNRFKEINDTLGHSIGDQVIKTLANRLKNGKVPFKLFARTGGDEFVM 379 + T + + F DL+RFK+IND+ GHS GD+V+K A RL+N R GGDEF++ Sbjct: 210 QRTGRQVCVMFLDLDRFKQINDSYGHSAGDEVLKLTARRLRNCVRISDSVGRLGGDEFIV 269 Query: 380 VFSNLAREHIPA-IAEHISGLISETLLISGAHLSLSTSIGVSLYPEHSLNKDDLLKFADM 438 + SN+ +H A IAE I +SE I G + S+G++ +P+H DLL+ AD+ Sbjct: 270 ILSNVEDQHAGAYIAEKILYSLSEPFPIGGTEVFCIPSVGITYFPDHGETAPDLLRNADV 329 Query: 439 SMYQAKSQEDANYFIFDWGL-REKLEQKTMIAKYLHSALEREEFVLHYQPQINAVTGKMI 497 +MY AK + Y IF + R L TM + H AL R+EF LH+QP++ A +I Sbjct: 330 AMYHAKQGGERRYAIFTPDMARRSLALLTMESDLRH-ALARDEFELHFQPKVRADL-TLI 387 Query: 498 GMEALVRWNHPEKGLIGPGTFITAVEEAGLMIQLTDWVLREVCRQLCEWRTMGLPLLRTS 557 G EAL+RW HPEKGLI PG FI EE+GL++ + W LRE C ++ WR GL + S Sbjct: 388 GAEALIRWRHPEKGLINPGDFIPLAEESGLILPIGRWTLREACDRVMSWRAEGLTIPSVS 447 Query: 558 INISNSWFYNRNLIENLLAVLDQYGLETSMLEFEITES--TALLEEHYPLLQQMRDHGIV 615 +N+S F ++ L+E + +L + GLE L+ EITE+ T +E L+ ++D G+ Sbjct: 448 VNVSPRQFQDQTLVETVRQILVETGLEPEALDLEITETVMTGDVEHAVGTLRALKDLGVT 507 Query: 616 VSIDDFGTKYSSLNYLKHFPVNKIKIDRTFITGIGVSSIDETIIKSIVYVASQLGYDLIA 675 +SIDDFGT YSSLNYLK FP++ +KID+TF+ + S D I+ +I+ +A LG+ ++A Sbjct: 508 LSIDDFGTGYSSLNYLKTFPIDTLKIDQTFVRDVLHSGKDAAIVSTIIALARNLGFSVVA 567 Query: 676 EGVETQEQLDFLIKHNCPHIQGFLFFPPLP 705 EGVE EQ +FL +C + QGFL+ PLP Sbjct: 568 EGVEEIEQAEFLGSRDCQNFQGFLYSRPLP 597 >ref|ZP_00034273.1| COG2199: FOG: GGDEF domain [Burkholderia fungorum] Length = 1036 Score = 334 bits (856), Expect = 5e-90, Method: Compositional matrix adjust. Identities = 180/434 (41%), Positives = 264/434 (60%), Gaps = 4/434 (0%) Query: 283 DLRVSKRLYEKLAHYDSLTGIPNRLYFMDEFERLLQSAEPT-QTYTLFFFDLNRFKEIND 341 DL + E A+YDSLT +PNR D + L A+ + + F DL+ FK +ND Sbjct: 591 DLMRYQEQLEYQANYDSLTRLPNRNLLRDRLQHALIVAQRHHKGVAVVFIDLDGFKNVND 650 Query: 342 TLGHSIGDQVIKTLANRLKNGKVPFKLFARTGGDEFVMVFSNLAREH-IPAIAEHISGLI 400 +LGHS+GD+++ +A RL AR GGDEFV+V ++ E + A E + I Sbjct: 651 SLGHSVGDRLLSVVAERLARCTRTSDTVARHGGDEFVIVMTDTVDEKSLIAWMERVRVSI 710 Query: 401 SETLLISGAHLSLSTSIGVSLYPEHSLNKDDLLKFADMSMYQAKSQEDANYFIFDWGLRE 460 SE + + G L + S+G SL+P+ + + L+K AD++MY+AK + + + Sbjct: 711 SEPVWLDGTELYVGCSMGASLFPQDGEDAETLMKKADLAMYRAKDMGRNTFQFYQPEMNA 770 Query: 461 KLEQKTMIAKYLHSALEREEFVLHYQPQINAVTGKMIGMEALVRWNHPEKGLIGPGTFIT 520 + + + L AL +F+LHYQPQ++ V+G+++G EALVRW PE GL+ P +FI Sbjct: 771 SAGTRLNLERRLRRALRDNQFLLHYQPQVDIVSGQIVGTEALVRWRDPEVGLVPPSSFIP 830 Query: 521 AVEEAGLMIQLTDWVLREVCRQLCEWRTMGLPLLRTSINISNSWFYNRNLIENLLAVLDQ 580 EE+GL+ L++WVLRE CRQ W+ GLP R S+N+S F R++ + ++ VLD+ Sbjct: 831 VAEESGLIGPLSEWVLREACRQNKAWQDEGLPPARVSVNLSARVFQQRDIAKLVMQVLDE 890 Query: 581 YGLETSMLEFEITESTAL--LEEHYPLLQQMRDHGIVVSIDDFGTKYSSLNYLKHFPVNK 638 GLE LE E+TEST + EE +L ++ GI ++IDDFGT YSSL+YLK FPV++ Sbjct: 891 TGLEPQYLELELTESTIMRNAEEAVSMLNELHALGIGLAIDDFGTGYSSLSYLKRFPVDR 950 Query: 639 IKIDRTFITGIGVSSIDETIIKSIVYVASQLGYDLIAEGVETQEQLDFLIKHNCPHIQGF 698 +KIDR+F++ IG+S DETI +I+ +A L +IAEGVET QLDFL + C +QGF Sbjct: 951 LKIDRSFVSDIGISGDDETITSAIIALAHSLKLQVIAEGVETSAQLDFLKERACDEMQGF 1010 Query: 699 LFFPPLPAEDIHKL 712 F PL + I +L Sbjct: 1011 YFAKPLSTDAISEL 1024 >ref|ZP_00082370.1| COG2200: FOG: EAL domain [Geobacter metallireducens] Length = 821 Score = 332 bits (852), Expect = 1e-89, Method: Compositional matrix adjust. Identities = 178/436 (40%), Positives = 263/436 (60%), Gaps = 6/436 (1%) Query: 283 DLRVSKRLYEK---LAHYDSLTGIPNRLYFMDEFERLLQSAEP-TQTYTLFFFDLNRFKE 338 D+ KR EK LA+YDSLTG+PNR MD + ++ A + L F DL+RFK+ Sbjct: 371 DITDRKRAEEKIRRLAYYDSLTGLPNRPLMMDRLSQAIRHARRHGEVVALLFLDLDRFKD 430 Query: 339 INDTLGHSIGDQVIKTLANRLKNGKVPFKLFARTGGDEFVMVFSNL-AREHIPAIAEHIS 397 +ND+ GH +GD++++ +A RL AR GGDEFV++ + A +A+ I Sbjct: 431 VNDSQGHRVGDRLLQEVAARLSGCTRESDTLARLGGDEFVIIAPGVRAATDASLLADKIL 490 Query: 398 GLISETLLISGAHLSLSTSIGVSLYPEHSLNKDDLLKFADMSMYQAKSQEDANYFIFDWG 457 ++E + L ++TSIG+ L+P N + LL+ ADM+MY AK + +Y F Sbjct: 491 AALAEPFTLDRQTLHIATSIGIVLFPSDGGNGEMLLQRADMAMYAAKDRGGNSYRFFSAE 550 Query: 458 LREKLEQKTMIAKYLHSALEREEFVLHYQPQINAVTGKMIGMEALVRWNHPEKGLIGPGT 517 + + ++ + + + AL EF L YQPQ+N T K++G+EALVRWNHP KG+I P Sbjct: 551 MNTRAVERHELEEDMRQALTNGEFFLMYQPQVNTGTWKVVGVEALVRWNHPRKGVILPSR 610 Query: 518 FITAVEEAGLMIQLTDWVLREVCRQLCEWRTMGLPLLRTSINISNSWFYNRNLIENLLAV 577 FI EE G + +L WVL E CRQ +W+ GLP L+ +N+ F + ++ + V Sbjct: 611 FIPVAEETGFISKLGRWVLEEACRQGAQWQHDGLPPLQMGVNLCARQFTHEEIVGTVSDV 670 Query: 578 LDQYGLETSMLEFEITESTAL-LEEHYPLLQQMRDHGIVVSIDDFGTKYSSLNYLKHFPV 636 L + GL + +LE E+TE+ + E L Q++ G+ ++IDDFGT YSS NYLKHFP+ Sbjct: 671 LQKTGLPSELLEIELTETMLMDSETAVSTLGQLKALGVRLAIDDFGTGYSSFNYLKHFPL 730 Query: 637 NKIKIDRTFITGIGVSSIDETIIKSIVYVASQLGYDLIAEGVETQEQLDFLIKHNCPHIQ 696 ++IKID++FI IG S + II++IV VA LG +++AEGVET+EQL +L+ C IQ Sbjct: 731 DRIKIDQSFIRDIGQSRGNGAIIEAIVAVARSLGPEVLAEGVETEEQLRYLMSITCADIQ 790 Query: 697 GFLFFPPLPAEDIHKL 712 GF F PL +D+ +L Sbjct: 791 GFYFSRPLLPDDVSRL 806 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.321 0.138 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 972,130,663 Number of extensions: 38923108 Number of successful extensions: 100077 Number of sequences better than 1.0e-05: 2736 Number of HSP's gapped: 95634 Number of HSP's successfully gapped: 2744 Length of query: 714 Length of database: 757,237,578 Length adjustment: 133 Effective length of query: 581 Effective length of database: 396,129,411 Effective search space: 230151187791 Effective search space used: 230151187791 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)