BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0258.0005.p (324 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_535381.1| NAD binding oxidoreductase [Agrobacterium tumef... 239 4e-62 ref|NP_395943.1| AGR_pAT_7p [Agrobacterium tumefaciens] >gi|1516... 239 5e-62 sp|P49305|YMO1_RHIME Hypothetical 36.4 kDa protein in mocC-mocA ... 226 5e-58 gb|EAD61742.1| unknown [environmental sequence] 224 3e-57 gb|EAJ12734.1| unknown [environmental sequence] 223 7e-57 >ref|NP_535381.1| NAD binding oxidoreductase [Agrobacterium tumefaciens str. C58 (U. Washington)] pir||AC3160 NAD binding oxidoreductase Atu5004 [imported] - Agrobacterium tumefaciens (strain C58, Dupont) plasmid AT gb|AAD41367.1| putative oxidoreductase [Agrobacterium tumefaciens] gb|AAL45697.1| NAD binding oxidoreductase [Agrobacterium tumefaciens str. C58 (U. Washington)] Length = 335 Score = 239 bits (611), Expect = 4e-62, Method: Compositional matrix adjust. Identities = 123/266 (46%), Positives = 170/266 (63%), Gaps = 8/266 (3%) Query: 1 MGCAQIATGSVMPAIQESETGIIQAVASRGLDKSSAVAEEFGVAQAYGSYEELLADPEVD 60 +GCA IA +V+PAIQ S G + A+ASR K+ + FG+A++YGSYE+LLADPE+D Sbjct: 10 LGCAGIAAKAVIPAIQSSRLGRVVAIASRDAAKAKEMTARFGIARSYGSYEDLLADPEID 69 Query: 61 AVYIPLPNHLHREWVIRAAEAGKHVLCEKPIALNSREAAEMVEACRRAGVHLAEAYMYRH 120 A+Y PLPNHLH I+A E GK VLCEKPIAL++ +A E+ A + A + +AEA+M RH Sbjct: 70 AIYNPLPNHLHVPMTIKALEQGKPVLCEKPIALDAAQARELATAQKAASLPVAEAFMVRH 129 Query: 121 HPRIAEVQEIIASGEIGDLRSIRGTFTYNDAGDTSNIRFKSAWGGGSLYDVGCYPLSAAR 180 HP+ + +I G +G++R+I+ F+Y D N+R + GGG L+DVGCY ++ AR Sbjct: 130 HPQWKKAHALITEGRLGEVRAIQTIFSYY-LDDPGNVRNQVDIGGGGLFDVGCYAINTAR 188 Query: 181 LLFGAEPEAATVQAIFSPEHDNV---DMMASGLVEFPGGVSLIFDCGMWAYNRQLLEVLG 237 LF AEP ++AI E D V D + SGL+ FP G L F C Q + VLG Sbjct: 189 FLFDAEP----LRAIALMERDPVFGTDRLTSGLLAFPEGKQLAFTCSTQLSLTQKVTVLG 244 Query: 238 TEGRIELPMPFNARFDDAEFFVYSGG 263 T GR+E+P+PFNA D+ ++ G Sbjct: 245 TRGRLEIPIPFNAPADEPTVLIFDDG 270 >ref|NP_395943.1| AGR_pAT_7p [Agrobacterium tumefaciens] gb|AAK90384.1| AGR_pAT_7p [Agrobacterium tumefaciens str. C58 (Cereon)] Length = 373 Score = 239 bits (611), Expect = 5e-62, Method: Compositional matrix adjust. Identities = 123/266 (46%), Positives = 170/266 (63%), Gaps = 8/266 (3%) Query: 1 MGCAQIATGSVMPAIQESETGIIQAVASRGLDKSSAVAEEFGVAQAYGSYEELLADPEVD 60 +GCA IA +V+PAIQ S G + A+ASR K+ + FG+A++YGSYE+LLADPE+D Sbjct: 48 LGCAGIAAKAVIPAIQSSRLGRVVAIASRDAAKAKEMTARFGIARSYGSYEDLLADPEID 107 Query: 61 AVYIPLPNHLHREWVIRAAEAGKHVLCEKPIALNSREAAEMVEACRRAGVHLAEAYMYRH 120 A+Y PLPNHLH I+A E GK VLCEKPIAL++ +A E+ A + A + +AEA+M RH Sbjct: 108 AIYNPLPNHLHVPMTIKALEQGKPVLCEKPIALDAAQARELATAQKAASLPVAEAFMVRH 167 Query: 121 HPRIAEVQEIIASGEIGDLRSIRGTFTYNDAGDTSNIRFKSAWGGGSLYDVGCYPLSAAR 180 HP+ + +I G +G++R+I+ F+Y D N+R + GGG L+DVGCY ++ AR Sbjct: 168 HPQWKKAHALITEGRLGEVRAIQTIFSYY-LDDPGNVRNQVDIGGGGLFDVGCYAINTAR 226 Query: 181 LLFGAEPEAATVQAIFSPEHDNV---DMMASGLVEFPGGVSLIFDCGMWAYNRQLLEVLG 237 LF AEP ++AI E D V D + SGL+ FP G L F C Q + VLG Sbjct: 227 FLFDAEP----LRAIALMERDPVFGTDRLTSGLLAFPEGKQLAFTCSTQLSLTQKVTVLG 282 Query: 238 TEGRIELPMPFNARFDDAEFFVYSGG 263 T GR+E+P+PFNA D+ ++ G Sbjct: 283 TRGRLEIPIPFNAPADEPTVLIFDDG 308 >sp|P49305|YMO1_RHIME Hypothetical 36.4 kDa protein in mocC-mocA intergenic region (ORF334) pir||S51570 hypothetical protein 334 - Rhizobium meliloti emb|CAA55267.1| unnamed protein product [Sinorhizobium meliloti] Length = 334 Score = 226 bits (576), Expect = 5e-58, Method: Compositional matrix adjust. Identities = 117/263 (44%), Positives = 165/263 (62%), Gaps = 2/263 (0%) Query: 1 MGCAQIATGSVMPAIQESETGIIQAVASRGLDKSSAVAEEFGVAQAYGSYEELLADPEVD 60 +G A IA V+P++ +E + A+ASR LD++ A A FG+ ++YGSY+E+LADPE++ Sbjct: 9 IGTAAIAVEKVIPSMLSAEGLEVVAIASRDLDRARAAATRFGIGRSYGSYDEILADPEIE 68 Query: 61 AVYIPLPNHLHREWVIRAAEAGKHVLCEKPIALNSREAAEMVEACRRAGVHLAEAYMYRH 120 AVYIPLPNHLH W IRAAEAGKHVLCEKP+AL+ E + +++ R G + EA M R Sbjct: 69 AVYIPLPNHLHVHWAIRAAEAGKHVLCEKPLALDVEELSRLIDCRDRTGRRIQEAVMIRA 128 Query: 121 HPRIAEVQEIIASGEIGDLRSIRGTFTYNDAGDTSNIRFKSAWGGGSLYDVGCYPLSAAR 180 HP+ E+ +I+ASGEIG++R+I+G FT + D +I ++ GGG+LYD+G YP++AAR Sbjct: 129 HPQWDEIFDIVASGEIGEVRAIQGVFTEVNL-DPKSIVNDASIGGGALYDLGVYPIAAAR 187 Query: 181 LLFGAEPEAATVQAIFSPEHDNVDMMASGLVEFPGGVSLIFDCGMWAYNRQLLEVLGTEG 240 ++F AEPE + P +D + S ++ FPGG R +E+ GT Sbjct: 188 MVFAAEPERVFAVSDLDPVF-GIDRLTSAVLLFPGGRQATLVVSTQLALRHNVEIFGTRK 246 Query: 241 RIELPMPFNARFDDAEFFVYSGG 263 I L PFN DD V G Sbjct: 247 SISLKNPFNPTPDDHCRIVLDNG 269 >gb|EAD61742.1| unknown [environmental sequence] Length = 275 Score = 224 bits (570), Expect = 3e-57, Method: Compositional matrix adjust. Identities = 123/251 (49%), Positives = 171/251 (68%), Gaps = 5/251 (1%) Query: 1 MGCAQIATGSVMPAIQESETGIIQAVASRGLDKSSAVAEEFGVAQAYGSYEELLADPEVD 60 + A+I T V+PA+Q+SETG I A+ASR + S V E G+ +AYGSYEELLADPE+D Sbjct: 9 ISSAKIGTEKVIPAMQQSETGEIMALASRNEETSKRVCENLGIPKAYGSYEELLADPEID 68 Query: 61 AVYIPLPNHLHREWVIRAAEAGKHVLCEKPIALNSREAAEMVEACRRAGVHLAEAYMYRH 120 A+Y PLPNHLH W I+AA++GKHVLCEKPIALN+ EA++++E + GV + EA+M R Sbjct: 69 AIYNPLPNHLHVPWTIKAAKSGKHVLCEKPIALNAEEASKLLEIQKDTGVLIVEAFMVRD 128 Query: 121 HPRIAEVQEIIASGEIGDLRSIRGTFTYNDAGDTSNIRFKSAWGGGSLYDVGCYPLSAAR 180 +P+ VQE + GEIG+LR+I+ F+Y + D NIR GGG +YD+GCYP+ +R Sbjct: 129 NPQWHWVQEQVKCGEIGELRAIQSAFSYMNR-DPQNIRNMPDIGGGGIYDIGCYPVFISR 187 Query: 181 LLFGAEPEAATVQAIFSPEHD-NVDMMASGLVEFPGGVSLIFDCGMWAYNRQLLEVLGTE 239 ++FG EP V A+ + D D +ASG+++FP G S F C Q +++ GT+ Sbjct: 188 MVFGEEP--LEVSALIEKDPDFQTDRLASGMMKFPRGQSS-FICSTQLVPYQRVQIFGTK 244 Query: 240 GRIELPMPFNA 250 RIE+ +PFNA Sbjct: 245 KRIEVEVPFNA 255 >gb|EAJ12734.1| unknown [environmental sequence] Length = 323 Score = 223 bits (567), Expect = 7e-57, Method: Compositional matrix adjust. Identities = 122/248 (49%), Positives = 170/248 (68%), Gaps = 5/248 (2%) Query: 4 AQIATGSVMPAIQESETGIIQAVASRGLDKSSAVAEEFGVAQAYGSYEELLADPEVDAVY 63 A+I T V+PA+Q+SE+G I A+ASR + S V E G+ +AYGSYEELLADPE+DA+Y Sbjct: 2 AKIGTEKVIPAMQQSESGEIMALASRSKETSKRVCENLGIPKAYGSYEELLADPEIDAIY 61 Query: 64 IPLPNHLHREWVIRAAEAGKHVLCEKPIALNSREAAEMVEACRRAGVHLAEAYMYRHHPR 123 PLPNHLH W I+AA++GKHVLCEKPIALN+ EA++++E + V + EA+M R +P+ Sbjct: 62 NPLPNHLHVPWTIKAAKSGKHVLCEKPIALNADEASQLLEVQKDTRVLIVEAFMVRDNPQ 121 Query: 124 IAEVQEIIASGEIGDLRSIRGTFTYNDAGDTSNIRFKSAWGGGSLYDVGCYPLSAARLLF 183 VQE + SGEIG+LR+I+ F+Y + D NIR GGG +YD+GCYP+ +R++F Sbjct: 122 WLWVQEQVNSGEIGELRAIQSAFSYMNR-DPQNIRNMPDIGGGGIYDIGCYPVFISRMVF 180 Query: 184 GAEPEAATVQAIFSPEHD-NVDMMASGLVEFPGGVSLIFDCGMWAYNRQLLEVLGTEGRI 242 G EP V A+ + D D +ASG+++FP G S F C Q +++ GT+ RI Sbjct: 181 GEEP--LKVCALIEKDPDFQTDRLASGMMKFPSGQS-SFICSTQLVPYQRVQIFGTKKRI 237 Query: 243 ELPMPFNA 250 E+ +PFNA Sbjct: 238 EVEVPFNA 245 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.319 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 435,308,085 Number of extensions: 16910539 Number of successful extensions: 39566 Number of sequences better than 1.0e-05: 1176 Number of HSP's gapped: 39175 Number of HSP's successfully gapped: 1176 Length of query: 324 Length of database: 757,237,578 Length adjustment: 126 Effective length of query: 198 Effective length of database: 415,135,104 Effective search space: 82196750592 Effective search space used: 82196750592 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 126 (53.1 bits)