BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0258.0001.p (669 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00021458.1| COG2200: FOG: EAL domain [Ralstonia metallidu... 402 e-110 ref|ZP_00092157.1| COG2200: FOG: EAL domain [Azotobacter vinelan... 389 e-106 ref|NP_953093.1| sensory box/GGDEF family protein [Geobacter sul... 361 3e-98 ref|ZP_00060071.1| COG2200: FOG: EAL domain [Clostridium thermoc... 357 4e-97 ref|ZP_00080135.1| COG2200: FOG: EAL domain [Geobacter metallire... 356 1e-96 >ref|ZP_00021458.1| COG2200: FOG: EAL domain [Ralstonia metallidurans] Length = 709 Score = 402 bits (1032), Expect = e-110, Method: Compositional matrix adjust. Identities = 233/654 (35%), Positives = 353/654 (53%), Gaps = 22/654 (3%) Query: 37 SAGRVRRLWLLSSACVLGSGIWAMHFVGVMASYLPFKVSYHPGIAGCSLLISMCACYLSL 96 ++GR RLWL A +G GIW+MHF+G+++ LP + Y P I SLLI++ A +L Sbjct: 54 ASGRYARLWLAGGAVAMGLGIWSMHFLGMLSFSLPIPLGYDPAITAVSLLIAVVASAFAL 113 Query: 97 WVATMSPLRTGRXXXXXXXXXXXXXXXHYMGMYSMKLQAEIHYETLTQGLXXXXXXXXXX 156 + + L R HY GM ++++Q I Y+ L Sbjct: 114 RLVCLEHLPRRRLALGALVLGGAVASMHYTGMAALRMQPGIQYDPWLFLLSILIAVGASG 173 Query: 157 XXXFLFHKFKDY-AGFSRWKLYSAVFIGLAVTGMHYISLRASILEVDNWTGTR------- 208 ++ + + + + + +A+ +G AV MHY + A+ + + G Sbjct: 174 AALWIAFRLRRHMPRVHQLRFGAALVMGGAVAAMHYTGMAAARFPIGSVCGAAGGGLQGN 233 Query: 209 ----PLLIESDXXXXXXXXXXXXFM-----LAVSCGAVFLDRHVLERMAYHDPLTELPNR 259 P+L+ + M AV A+ + LE +A HD LT LPNR Sbjct: 234 TLALPILVITICVLAVALITSVLDMRLEMRTAVLADALGVANEELEFLALHDKLTHLPNR 293 Query: 260 HGLERYFKGEFFGG--HSG--AVFFVDLDRFKSINDTLGHDIGDMLLQEVAGRLRGVISA 315 LE F H G AV FVDLD FK +NDT GH +GD LL E+A RLR +S+ Sbjct: 294 VLLEDRFTLAIQAAARHHGRFAVLFVDLDGFKGVNDTYGHQVGDGLLVEIANRLRASVSS 353 Query: 316 KGKVFRLGGDEFLIALPVCTMEDAREEAQHILRELKKAYSIEGNELYVTASVGISMTPLH 375 + + R+GGDEF++ + V EDA A ++ L++ ++ G+ ++V+ S+GI++ P+ Sbjct: 354 EDTIARVGGDEFVLLVHVDEPEDAGVVAGKLIDVLREPANVAGHMVHVSGSIGIAIYPVD 413 Query: 376 GTDRSALMKAADTALYTSKDSGKNKFSVFDQEMNRHQLRRMSLEKDLRKALARSEFMVVY 435 G D+ ALM AD A+Y +K SG+N F++ MN + L +DLR AL + + Y Sbjct: 414 GQDQDALMTNADAAMYHAKASGRNASYFFERSMNHQARAQQMLIQDLRAALRNGQLQLHY 473 Query: 436 QPKWDSLMNVTVGLEALLRWRHPEHGIISPAEFIPIAEETGLIVPITYWMLHDVCGQNKL 495 QPK+ + NV VG EALLRW HP G++ P +FI +AE+TG IVPI W+L++ C Q L Sbjct: 474 QPKFSAADNVLVGAEALLRWNHPVQGLLMPDQFITLAEKTGAIVPIGTWVLNEACRQLAL 533 Query: 496 WHKACVANVAVSINMSARMFEGGSLYDVVEEALVRSGLEPHFLELEITESIAMNNMEETV 555 WH A+ ++++N+SA F + + V EAL R +EP L LEITE+ AM ++E T+ Sbjct: 534 WHDMGHAHWSMAVNLSALQFCHAGMVESVAEALARHKVEPRRLTLEITETTAMRDVETTL 593 Query: 556 AQLSKLRNLGVRVSLDDFGTGFSSLGNLDEIPVNTLKIDQVFIRK-SKMHXXXXXXXXXX 614 A +++L +GVR+++DDFGTG+SSL +L IP + LKID+ F+R ++ Sbjct: 594 AIMTQLDEMGVRIAIDDFGTGYSSLLHLKRIPASELKIDRGFVRDLAEDSEDAAIVSAIV 653 Query: 615 XXXXXLNMEVVAEGVETPEQIELLQSLGCRVMQGFYYGRPMPVSELGQWFIENT 668 LN++VVAEGVET Q L LGC +QG+ GRPMP ++ + + + Sbjct: 654 ALGRTLNLQVVAEGVETTAQRSFLAGLGCDALQGYLLGRPMPAAQFTELALADV 707 >ref|ZP_00092157.1| COG2200: FOG: EAL domain [Azotobacter vinelandii] Length = 716 Score = 389 bits (999), Expect = e-106, Method: Compositional matrix adjust. Identities = 233/665 (35%), Positives = 348/665 (52%), Gaps = 37/665 (5%) Query: 38 AGRV-------RRLWLLSSACVLGSGIWAMHFVGVMASYLPFKVSYHPGIAGCSLLISMC 90 AGRV R WL A +G+GIW+MHFVG++A LP ++ Y + SLLI++ Sbjct: 56 AGRVSTAQRGARLAWLAGGALAMGTGIWSMHFVGMLAFSLPIELGYDLWLTSASLLIAIA 115 Query: 91 ACYLSLWVATMSPLRTGRXXXXXXXXXXXXXXXHYMGMYSMKLQAEIHYETLTQGLXXXX 150 + +LW+ L R HY GM +M++ I Y+ L Sbjct: 116 SSTFALWLVCQRELPWPRLAMGASLMGAGIAAMHYTGMEAMRMVPGIVYDPAWFALSIVV 175 Query: 151 XXXXXXXXXFLFHKFKDYAGFSRWKLYS----AVFIGLAVTGMHYISLRASILEVDNWTG 206 ++ + + + S W ++S +V +G A+ GMHY + AS ++ G Sbjct: 176 AVLASGAALWIAFRLRWH---STWVVFSRAGASVAMGCAIVGMHYTGMAASQFPEGSYCG 232 Query: 207 TRPLLIESDXXXXXXXXXXXXFMLAVSCGAVFLDRHVLER-----------------MAY 249 I++ M A + LD + +R +A Sbjct: 233 AANSGIDTGWLSILVIMAALSVM-ATTLIISVLDSRLEQRTASLASALSKANSELMQLAL 291 Query: 250 HDPLTELPNRHGLERYFKGEFFGGHSG----AVFFVDLDRFKSINDTLGHDIGDMLLQEV 305 +D LT+LPNR L AV F+DLD FK+IND GH +GD+LL EV Sbjct: 292 YDNLTKLPNRMLLADRLGQAIEKARREHKVFAVLFIDLDGFKAINDAYGHHLGDLLLVEV 351 Query: 306 AGRLRGVISAKGKVFRLGGDEFLIALPVCTMEDAREEAQHILRELKKAYSIEGNELYVTA 365 R+RG + + + RLGGDE ++ + DA + A ++ +++ YSIE +EL+V+A Sbjct: 352 TQRIRGSVRGQDTIARLGGDELILLTEIGEPTDAAKIAGKLIETIRQPYSIEHHELFVSA 411 Query: 366 SVGISMTPLHGTDRSALMKAADTALYTSKDSGKNKFSVFDQEMNRHQLRRMSLEKDLRKA 425 SVGI++ P G L+ AD A+Y +K+ G++ + F+ MN + ++ L +DLR A Sbjct: 412 SVGIAVYPDDGLAPHELLVNADAAMYHAKEQGRDGYCFFENSMNANAQEQLRLLQDLRHA 471 Query: 426 LARSEFMVVYQPKWDSLMNVTVGLEALLRWRHPEHGIISPAEFIPIAEETGLIVPITYWM 485 L R E + YQPK + +G+EALLRW+HPE G++SP +F+P+AE+TGLIVPI W+ Sbjct: 472 LERDELRLHYQPKLIAPDGPIIGVEALLRWQHPERGLLSPDQFLPLAEKTGLIVPIGNWV 531 Query: 486 LHDVCGQNKLWHKACVANVAVSINMSARMFEGGSLYDVVEEALVRSGLEPHFLELEITES 545 L + C Q + W A + +++N+SA F L D V AL R GLE H L LEITES Sbjct: 532 LDEACAQIQRWRAAGHTSWNIAVNLSASQFAHAKLIDSVRTALQRHGLEAHNLTLEITES 591 Query: 546 IAMNNMEETVAQLSKLRNLGVRVSLDDFGTGFSSLGNLDEIPVNTLKIDQVFIRK-SKMH 604 AM + + ++A L +L +GV +S+DDFGTG+SSL L +P N LKID+ F+ + + Sbjct: 592 TAMRDADSSLAVLEQLAAMGVGISIDDFGTGYSSLLYLKRLPANELKIDRGFVNELGQGG 651 Query: 605 XXXXXXXXXXXXXXXLNMEVVAEGVETPEQIELLQSLGCRVMQGFYYGRPMPVSELGQWF 664 L +++VAEGVETPEQ + L LGC ++QG+ GRPMP +L + + Sbjct: 652 DDAAIVSAIIALGRTLGLKIVAEGVETPEQQKHLTRLGCNLLQGYLLGRPMPAEQLIECY 711 Query: 665 IENTA 669 E A Sbjct: 712 GETPA 716 >ref|NP_953093.1| sensory box/GGDEF family protein [Geobacter sulfurreducens PCA] gb|AAR35420.1| sensory box/GGDEF family protein [Geobacter sulfurreducens PCA] Length = 762 Score = 361 bits (927), Expect = 3e-98, Method: Compositional matrix adjust. Identities = 190/430 (44%), Positives = 263/430 (61%), Gaps = 7/430 (1%) Query: 242 HVLERMAYHDPLTELPNRHGLERYFKGEFFGGHSG----AVFFVDLDRFKSINDTLGHDI 297 + +MAY+D LT LPNR + +V F+DLDRFK INDTLGH + Sbjct: 319 ETIRQMAYYDSLTGLPNRRLFNDRLRQSLALAQRNKRLLSVLFLDLDRFKLINDTLGHAV 378 Query: 298 GDMLLQEVAGRLRGVISAKGK-VFRLGGDEFLIALPVCT-MEDAREEAQHILRELKKAYS 355 GD LL +VA RL+GV G V R GGDEF+I L V + ++DA + AQ I+ L+ + Sbjct: 379 GDKLLMQVAKRLKGVCRRDGDTVARQGGDEFIINLSVISDVKDALKVAQKIIDSLRTPFV 438 Query: 356 IEGNELYVTASVGISMTPLHGTDRSALMKAADTALYTSKDSGKNKFSVFDQEMNRHQLRR 415 I+G+EL++T S+GIS+ P G AL+K AD A+Y +K+ G+N ++ EMN R Sbjct: 439 IDGHELFITTSIGISVFPYDGRTSDALVKNADFAMYRAKEQGRNNCQLYTPEMNSKAFER 498 Query: 416 MSLEKDLRKALARSEFMVVYQPKWDSLMNVTVGLEALLRWRHPEHGIISPAEFIPIAEET 475 +SLE +LRK L R E ++ YQPK + +EAL+RW+HP G++ PA FIP+AEET Sbjct: 499 LSLENNLRKGLEREELVLYYQPKVNVEDGQISCMEALVRWQHPTMGLVPPARFIPLAEET 558 Query: 476 GLIVPITYWMLHDVCGQNKLWHKACVANVAVSINMSARMFEGGSLYDVVEEALVRSGLEP 535 GLIVP+ W+L C QN+ W KA + V++N+S R + G+L DVVE AL SGL P Sbjct: 559 GLIVPLGEWVLRTACRQNREWQKAGYPPMQVAVNISPRQLQQGNLVDVVERALSDSGLAP 618 Query: 536 HFLELEITESIAMNNMEETVAQLSKLRNLGVRVSLDDFGTGFSSLGNLDEIPVNTLKIDQ 595 +L LE+TESI M+ E T+A L +G+ +++DDFGTG+SSL L + P++ LKID+ Sbjct: 619 RWLVLEVTESIMMDGTESTLATFRVLERMGIHIAIDDFGTGYSSLHYLKKFPIHALKIDR 678 Query: 596 VFIRKSKMHXXXXXXXXXXXXXXX-LNMEVVAEGVETPEQIELLQSLGCRVMQGFYYGRP 654 FIR + LN++VVAEGVET EQ+E+L++L C MQG + RP Sbjct: 679 SFIRDITTNPDDEAITSAVITMAKGLNLKVVAEGVETVEQLEVLRALQCTHMQGHLFSRP 738 Query: 655 MPVSELGQWF 664 P + F Sbjct: 739 APAEQFAPVF 748 >ref|ZP_00060071.1| COG2200: FOG: EAL domain [Clostridium thermocellum ATCC 27405] Length = 862 Score = 357 bits (917), Expect = 4e-97, Method: Compositional matrix adjust. Identities = 177/425 (41%), Positives = 265/425 (62%), Gaps = 6/425 (1%) Query: 242 HVLERMAYHDPLTELPNRHGLERYFKGEFFGGHSG----AVFFVDLDRFKSINDTLGHDI 297 +L M Y+DPLT LPNR K A+ +DLD FK+INDTLGH + Sbjct: 420 EILYNMEYYDPLTGLPNRSLFSDRLKMALAQSKRNNEMCALIILDLDNFKAINDTLGHSV 479 Query: 298 GDMLLQEVAGRLRGVISAKGKVFRLGGDEFLIALP-VCTMEDAREEAQHILRELKKAYSI 356 GDMLL++VA +++G + V R+GGDEF+I P + + DA A IL++ ++ + + Sbjct: 480 GDMLLKQVAEKIKGYLREVDTVARIGGDEFIIIQPQIKDINDATRAADRILKKFQQPWIL 539 Query: 357 EGNELYVTASVGISMTPLHGTDRSALMKAADTALYTSKDSGKNKFSVFDQEMNRHQLRRM 416 EG E Y+TAS+GI + P G D + K ADTALY +K+ G+N + ++ + MN+ L+R+ Sbjct: 540 EGKEYYITASMGIGIYPNGGEDPQTIFKNADTALYRAKELGRNNYQLYTESMNQKVLQRL 599 Query: 417 SLEKDLRKALARSEFMVVYQPKWDSLMNVTVGLEALLRWRHPEHGIISPAEFIPIAEETG 476 +E LR+A+ + EF++ YQP+ D VG EALLRW HP++G++ P EFIP+AE++G Sbjct: 600 DIENSLRRAIEKEEFVLFYQPQIDIKTGKIVGFEALLRWYHPDYGLMPPMEFIPVAEDSG 659 Query: 477 LIVPITYWMLHDVCGQNKLWHKACVANVAVSINMSARMFEGGSLYDVVEEALVRSGLEPH 536 LIV I W+L C QNK W + + +S+N+SAR F+ ++ +V++ +GL P Sbjct: 660 LIVVIGEWVLETACRQNKKWIECGLEPHLISVNLSARQFQRSNIVEVIDRIRSSTGLAPE 719 Query: 537 FLELEITESIAMNNMEETVAQLSKLRNLGVRVSLDDFGTGFSSLGNLDEIPVNTLKIDQV 596 LELEITES AM ++ T+ L++LR G+RVSLDDFGTG+SSL L ++P++TLKID+ Sbjct: 720 LLELEITESTAMQDLSFTIDVLNQLRKKGIRVSLDDFGTGYSSLNYLRQLPIDTLKIDKS 779 Query: 597 FIRKSKMHXXXXXXXXXXXXXX-XLNMEVVAEGVETPEQIELLQSLGCRVMQGFYYGRPM 655 F++ + + L++ VVAEGVET EQ+ L+ C QG+ + +P+ Sbjct: 780 FVQDIRANSKEEAIAKTVISLAHKLDLTVVAEGVETKEQLLFLKKEKCDKAQGYLFSKPL 839 Query: 656 PVSEL 660 P E+ Sbjct: 840 PAEEI 844 >ref|ZP_00080135.1| COG2200: FOG: EAL domain [Geobacter metallireducens] Length = 633 Score = 356 bits (914), Expect = 1e-96, Method: Compositional matrix adjust. Identities = 186/424 (43%), Positives = 257/424 (60%), Gaps = 7/424 (1%) Query: 244 LERMAYHDPLTELPNRHGLERYFKGEFFGGHSG----AVFFVDLDRFKSINDTLGHDIGD 299 + +MAY+D LT LPNR + AV F+DLDRFK INDTLGH +GD Sbjct: 192 IRQMAYYDSLTGLPNRRLFNDRLRQSLALAQRHKRPLAVMFLDLDRFKLINDTLGHAVGD 251 Query: 300 MLLQEVAGRLRGVISAKGK-VFRLGGDEFLIAL-PVCTMEDAREEAQHILRELKKAYSIE 357 LL VA RLR +G V R GGDEF+I L + ++DA AQ I+ + + I+ Sbjct: 252 RLLMAVAKRLRNCCRREGDTVARQGGDEFIINLSAISDVKDAFTVAQKIIDVFRTPFVID 311 Query: 358 GNELYVTASVGISMTPLHGTDRSALMKAADTALYTSKDSGKNKFSVFDQEMNRHQLRRMS 417 G EL++T S+GIS+ P G AL+K AD A+Y +K+ G+N +F EMN R+S Sbjct: 312 GYELFITTSIGISVYPHDGRSGDALVKNADVAMYRAKEQGRNNCQLFAPEMNARAFERLS 371 Query: 418 LEKDLRKALARSEFMVVYQPKWDSLMNVTVGLEALLRWRHPEHGIISPAEFIPIAEETGL 477 LE D+R+AL R EF++ YQPK + +EAL+RW+HP G++ P+ FIP+AEETGL Sbjct: 372 LENDMRRALERDEFVLHYQPKVNVEHGQIACMEALVRWQHPSMGLVPPSRFIPLAEETGL 431 Query: 478 IVPITYWMLHDVCGQNKLWHKACVANVAVSINMSARMFEGGSLYDVVEEALVRSGLEPHF 537 IVP+ W+L C QN+ W A + V++N+S R G L + V L +GL P + Sbjct: 432 IVPLGEWVLRTACQQNRAWQDAGYPTMQVAVNLSPRQLRQGDLSETVARVLAETGLAPGW 491 Query: 538 LELEITESIAMNNMEETVAQLSKLRNLGVRVSLDDFGTGFSSLGNLDEIPVNTLKIDQVF 597 L +E+TESI M++ EET+ +L +GVR+S+DDFGTG+SSL L + PV+TLKID+ F Sbjct: 492 LVIEVTESILMDDAEETIRTFRRLDRMGVRISIDDFGTGYSSLHYLKKFPVHTLKIDRSF 551 Query: 598 IRK-SKMHXXXXXXXXXXXXXXXLNMEVVAEGVETPEQIELLQSLGCRVMQGFYYGRPMP 656 +R+ + LN+ VVAEGVET EQ+E+L++L CR MQGF + P P Sbjct: 552 VREIASNPDDEAIAGAVISMAKGLNLGVVAEGVETVEQMEVLRALNCRYMQGFLFSEPAP 611 Query: 657 VSEL 660 + Sbjct: 612 AEQF 615 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.324 0.138 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 855,842,164 Number of extensions: 31702044 Number of successful extensions: 96693 Number of sequences better than 1.0e-05: 2773 Number of HSP's gapped: 91351 Number of HSP's successfully gapped: 2794 Length of query: 669 Length of database: 757,237,578 Length adjustment: 132 Effective length of query: 537 Effective length of database: 398,844,510 Effective search space: 214179501870 Effective search space used: 214179501870 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.5 bits) S2: 129 (54.3 bits)