BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0256.0055.p (340 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_815450.1| glycerol-3-phosphate dehydrogenase (NAD(P)+) [E... 358 1e-97 ref|NP_692717.1| glycerol-3-phosphate dehydrogenase [NAD(P)H] [O... 355 1e-96 ref|NP_831284.1| Glycerol-3-phosphate dehydrogenase [NAD(P)+] [B... 353 2e-96 ref|NP_242506.1| NAD(P)H-dependent glycerol-3-phosphate dehydrog... 352 8e-96 ref|NP_465460.1| similar to NAD(P)H-dependent glycerol-3-phospha... 350 3e-95 >ref|NP_815450.1| glycerol-3-phosphate dehydrogenase (NAD(P)+) [Enterococcus faecalis V583] sp|Q834C1|GPDA_ENTFA Glycerol-3-phosphate dehydrogenase [NAD(P)+] (NAD(P)H-dependent glycerol-3-phosphate dehydrogenase) gb|AAO81520.1| glycerol-3-phosphate dehydrogenase (NAD(P)+) [Enterococcus faecalis V583] Length = 340 Score = 358 bits (919), Expect = 1e-97, Method: Compositional matrix adjust. Identities = 186/329 (56%), Positives = 231/329 (70%), Gaps = 1/329 (0%) Query: 1 VLVAGSWGTALASVLAANHPEVYLWTRKPEQAAEINNSHTNQHFLPGITLPPNIIATTDM 60 VL GSWGTALA VLA N EV +W KPEQ EIN HTN+H+LP + LP +I ATTD+ Sbjct: 7 VLGPGSWGTALAQVLAENGHEVCIWGNKPEQIDEINTKHTNKHYLPELILPTSIQATTDL 66 Query: 61 ETAVKDSKAVVIVAPSSAMRQVAGALKPFWKEGMLCIHATKGFETETVKRMSTVISEELG 120 TA+ D AV+ V P+ A+R VA + K + IHA+KG E T KR+S VI+EE+ Sbjct: 67 ATALVDVDAVLFVVPTKAIRSVAQEVAQHLKTKPIIIHASKGLEQGTHKRISEVIAEEIP 126 Query: 121 CSEGE-IVVLSGPSHAEEVVRLCPTTVVVXXXXXXXXXXXXXLFINNDFRVYTNRDQVGV 179 + + IVVLSGPSHAEEV TT+ LF+N+ FR+YTN D +GV Sbjct: 127 AEKRQGIVVLSGPSHAEEVAVHDITTITAASENLADAVYVQELFMNDYFRIYTNDDVIGV 186 Query: 180 ELAGALKNIIALGAGLSDGLGFGDNAKAALLTRGLAEISRVGVELGANPLTFSGLAGLGD 239 E ALKNIIALGAG GLGFGDNAKAA++TRGLAEISR+GV +GANPLTF GL+G+GD Sbjct: 187 ETGAALKNIIALGAGAIHGLGFGDNAKAAIMTRGLAEISRLGVAMGANPLTFIGLSGVGD 246 Query: 240 LVVTATSKHSRNWRAGSMLGQGKPLAEVLESMGMVVEGIRTTKAAYAISLNLNVQMPITD 299 L+VT TS HSRNWRAG++LG+G L EVLE+MGM+VEG+ TTKAAY ++ L V+MPIT+ Sbjct: 247 LIVTCTSVHSRNWRAGNLLGKGHKLDEVLENMGMIVEGVSTTKAAYELAQQLEVEMPITE 306 Query: 300 QIYHVLFQGRTPRSAVEALMGRDRKTEME 328 IY+VL+ + A + +M RD KTE E Sbjct: 307 TIYNVLYNDEDVQQAAKEIMLRDGKTENE 335 >ref|NP_692717.1| glycerol-3-phosphate dehydrogenase [NAD(P)H] [Oceanobacillus iheyensis HTE831] sp|Q8EQA9|GPDA_OCEIH Glycerol-3-phosphate dehydrogenase [NAD(P)+] (NAD(P)H-dependent glycerol-3-phosphate dehydrogenase) dbj|BAC13752.1| glycerol-3-phosphate dehydrogenase [NAD(P)H] [Oceanobacillus iheyensis HTE831] Length = 344 Score = 355 bits (910), Expect = 1e-96, Method: Compositional matrix adjust. Identities = 179/331 (54%), Positives = 227/331 (68%), Gaps = 1/331 (0%) Query: 1 VLVAGSWGTALASVLAANHPEVYLWTRKPEQAAEINNSHTNQHFLPGITLPPNIIATTDM 60 VL AGSWGTALA VLA N EV LW+ + E A IN +H N +L + +P I A +DM Sbjct: 6 VLGAGSWGTALAIVLADNGHEVRLWSHRKEHAETINETHRNHKYLD-VDIPNEITAYSDM 64 Query: 61 ETAVKDSKAVVIVAPSSAMRQVAGALKPFWKEGMLCIHATKGFETETVKRMSTVISEELG 120 V AVVIV P+ A+R+V L + IHATKG E ++KR+S +I EE+ Sbjct: 65 HQTVSGVDAVVIVVPTKAIREVCSELNKWMDSKTTVIHATKGIEPVSLKRVSEMIEEEMD 124 Query: 121 CSEGEIVVLSGPSHAEEVVRLCPTTVVVXXXXXXXXXXXXXLFINNDFRVYTNRDQVGVE 180 +IVVLSGPSHAEEV PTTV LF ++ FRVYT+ D +G+E Sbjct: 125 IKYEDIVVLSGPSHAEEVALRHPTTVTTSSINLEKAKHAQDLFSSDSFRVYTSDDILGIE 184 Query: 181 LAGALKNIIALGAGLSDGLGFGDNAKAALLTRGLAEISRVGVELGANPLTFSGLAGLGDL 240 L GALKNIIALGAG+SDGLGFGDNAKAAL+TRGLAEI+R+G LGANPL+F GL+G+GDL Sbjct: 185 LGGALKNIIALGAGISDGLGFGDNAKAALITRGLAEIARLGTSLGANPLSFLGLSGVGDL 244 Query: 241 VVTATSKHSRNWRAGSMLGQGKPLAEVLESMGMVVEGIRTTKAAYAISLNLNVQMPITDQ 300 +VT TS HSRNWRAG++LG+G L +VLE MGMVVEG+RT KAA+ + + NV MPIT Sbjct: 245 IVTCTSVHSRNWRAGNLLGKGNQLEDVLEQMGMVVEGVRTVKAAHQFAKDQNVDMPITSG 304 Query: 301 IYHVLFQGRTPRSAVEALMGRDRKTEMEAIS 331 I+ +LF+ + P+ VE LM R ++ EM+ ++ Sbjct: 305 IFQILFENKNPKDVVEQLMNRGKRDEMDDLA 335 >ref|NP_831284.1| Glycerol-3-phosphate dehydrogenase [NAD(P)+] [Bacillus cereus ATCC 14579] sp|Q81FR4|GPDA_BACCR Glycerol-3-phosphate dehydrogenase [NAD(P)+] (NAD(P)H-dependent glycerol-3-phosphate dehydrogenase) gb|AAP08485.1| Glycerol-3-phosphate dehydrogenase [NAD(P)+] [Bacillus cereus ATCC 14579] Length = 340 Score = 353 bits (907), Expect = 2e-96, Method: Compositional matrix adjust. Identities = 187/331 (56%), Positives = 230/331 (69%), Gaps = 1/331 (0%) Query: 1 VLVAGSWGTALASVLAANHPEVYLWTRKPEQAAEINNSHTNQHFLPGITLPPNIIATTDM 60 V+ AGSWGTALA VLA N +V +W +PE EIN H N +LPGITLP I+A + + Sbjct: 6 VVGAGSWGTALAMVLADNGHDVRIWGNRPELMDEINTKHENSRYLPGITLPSTIVAYSSL 65 Query: 61 ETAVKDSKAVVIVAPSSAMRQVAGALKPFWKEGMLCIHATKGFETETVKRMSTVISEELG 120 E A+ D V++V P+ A R V +K E + IHA+KG E T KR+S VI EE+ Sbjct: 66 EEALVDVNTVLLVVPTKAYRDVLQEMKEIVTEPITWIHASKGIEPGTSKRISEVIEEEIP 125 Query: 121 CSE-GEIVVLSGPSHAEEVVRLCPTTVVVXXXXXXXXXXXXXLFINNDFRVYTNRDQVGV 179 ++VVLSGPSHAEEV TTV LF+N+ FRVYTN D VGV Sbjct: 126 EHLIKDVVVLSGPSHAEEVGLRQATTVTSAAKRMEAAEEVQDLFMNSYFRVYTNPDIVGV 185 Query: 180 ELAGALKNIIALGAGLSDGLGFGDNAKAALLTRGLAEISRVGVELGANPLTFSGLAGLGD 239 EL GALKNIIAL AG++DGLG GDNAKAAL+TRGL EI+R+G ++G NPLTF+GL G+GD Sbjct: 186 ELGGALKNIIALAAGITDGLGLGDNAKAALMTRGLTEIARLGRKMGGNPLTFAGLTGMGD 245 Query: 240 LVVTATSKHSRNWRAGSMLGQGKPLAEVLESMGMVVEGIRTTKAAYAISLNLNVQMPITD 299 L+VT TS HSRNWRAG+MLG+G L EVLESMGMVVEG+RTTKAA+ ++ + V+MPIT Sbjct: 246 LIVTCTSVHSRNWRAGNMLGKGHSLEEVLESMGMVVEGVRTTKAAHELAEKMEVEMPITA 305 Query: 300 QIYHVLFQGRTPRSAVEALMGRDRKTEMEAI 330 +Y VLF G + AV +LMGR RK E+EAI Sbjct: 306 ALYDVLFNGNNVKDAVGSLMGRVRKHEVEAI 336 >ref|NP_242506.1| NAD(P)H-dependent glycerol-3-phosphate dehydrogenase [Bacillus halodurans] sp|Q9KCD2|GPDA_BACHD Glycerol-3-phosphate dehydrogenase [NAD(P)+] (NAD(P)H-dependent glycerol-3-phosphate dehydrogenase) pir||H83854 NAD(P)H-dependent glycerol-3-phosphate dehydrogenase gpsA [imported] - Bacillus halodurans (strain C-125) dbj|BAB05359.1| NAD(P)H-dependent glycerol-3-phosphate dehydrogenase [Bacillus halodurans] Length = 345 Score = 352 bits (903), Expect = 8e-96, Method: Compositional matrix adjust. Identities = 184/338 (54%), Positives = 235/338 (69%), Gaps = 2/338 (0%) Query: 1 VLVAGSWGTALASVLAANHPEVYLWTRKPEQAAEINNSHTNQHFLPGITLPPNIIATTDM 60 V+ AGSWGTAL+ VLA N V L R+ E A EIN HTN+ +LP I +P +I+AT M Sbjct: 6 VIGAGSWGTALSLVLADNGHHVTLVARREEIAREINERHTNETYLPSIEIPSSIVATCSM 65 Query: 61 ETAVKDSKAVVIVAPSSAMRQVAGALKPFWKEGMLCIHATKGFETETVKRMSTVISEELG 120 E + D + +V+V P+ A+RQ +L K + +HA+KG E + R+S +I EEL Sbjct: 66 EEII-DVEIIVLVVPTKAIRQAVRSLNDVLKWPVTIVHASKGIEPGSHLRISEMIEEELE 124 Query: 121 CSE-GEIVVLSGPSHAEEVVRLCPTTVVVXXXXXXXXXXXXXLFINNDFRVYTNRDQVGV 179 + E+VVLSGPSHAEEV PTTV V LF+N FRVYTN D +GV Sbjct: 125 PTLLKEVVVLSGPSHAEEVSLRQPTTVTVSSKSLSTTKQIQDLFMNQQFRVYTNEDLIGV 184 Query: 180 ELAGALKNIIALGAGLSDGLGFGDNAKAALLTRGLAEISRVGVELGANPLTFSGLAGLGD 239 E+ GALKNIIAL GL++GLG+GDN KAA++TRGLAEI R+GV+LGA+PLTF+GL+GLGD Sbjct: 185 EIGGALKNIIALACGLTNGLGYGDNTKAAIMTRGLAEIGRLGVKLGASPLTFAGLSGLGD 244 Query: 240 LVVTATSKHSRNWRAGSMLGQGKPLAEVLESMGMVVEGIRTTKAAYAISLNLNVQMPITD 299 L+VT TS HSRNWRAG MLG+GK AEV ESMGMVVEGIRTT+AA+ ++ L ++MPIT Sbjct: 245 LIVTCTSIHSRNWRAGQMLGKGKSPAEVEESMGMVVEGIRTTQAAHELAQKLQIEMPITS 304 Query: 300 QIYHVLFQGRTPRSAVEALMGRDRKTEMEAISQETWEQ 337 +Y VLF+G+ P A + LMGR +K EME + +Q Sbjct: 305 ALYSVLFEGKKPEHAADELMGRVKKHEMENLHDVVSDQ 342 >ref|NP_465460.1| similar to NAD(P)H-dependent glycerol-3-phosphate dehydrogenase [Listeria monocytogenes EGD-e] sp|Q8Y5W9|GPDA_LISMO Glycerol-3-phosphate dehydrogenase [NAD(P)+] (NAD(P)H-dependent glycerol-3-phosphate dehydrogenase) pir||AH1316 NAD(P)H-dependent glycerol-3-phosphate dehydrogenase homolog gpsA [imported] - Listeria monocytogenes (strain EGD-e) emb|CAD00014.1| gpsA [Listeria monocytogenes] Length = 338 Score = 350 bits (897), Expect = 3e-95, Method: Compositional matrix adjust. Identities = 176/330 (53%), Positives = 229/330 (69%), Gaps = 1/330 (0%) Query: 1 VLVAGSWGTALASVLAANHPEVYLWTRKPEQAAEINNSHTNQHFLPGITLPPNIIATTDM 60 +L AGSWGT LA VLA N+ + +W + EIN SHTN H+LP I LP + AT + Sbjct: 8 ILGAGSWGTGLALVLADNNHKPVIWGNLDKIVNEINESHTNSHYLPDIILPTEVKATLSL 67 Query: 61 ETAVKDSKAVVIVAPSSAMRQVAGALKPFWKEGMLCIHATKGFETETVKRMSTVISEELG 120 + A+ ++ VVI P++AMR V L KE + +H +KG E ET RMS VI EE+ Sbjct: 68 DEAIDGAEIVVIAIPTNAMRIVCKQLNEALKEPTILVHVSKGIEPETNLRMSEVIEEEID 127 Query: 121 CSEGE-IVVLSGPSHAEEVVRLCPTTVVVXXXXXXXXXXXXXLFINNDFRVYTNRDQVGV 179 ++ + +VVLSGPSHAEEV PTT+ FINN+ R+YTN D +G Sbjct: 128 AAKRKALVVLSGPSHAEEVALRHPTTLCASCKDLSAAEIVQDRFINNNLRIYTNDDVIGA 187 Query: 180 ELAGALKNIIALGAGLSDGLGFGDNAKAALLTRGLAEISRVGVELGANPLTFSGLAGLGD 239 E+ GALKNIIALGAG+SDGLG+GDNAKAAL+TRG+AEI+R+GV +G+NP TF GL G+GD Sbjct: 188 EIGGALKNIIALGAGISDGLGYGDNAKAALMTRGMAEITRLGVAVGSNPQTFYGLTGIGD 247 Query: 240 LVVTATSKHSRNWRAGSMLGQGKPLAEVLESMGMVVEGIRTTKAAYAISLNLNVQMPITD 299 L+VT TS HSRNWRAG+MLG+G+ L EVLE MGMVVEG+RT KA + + L++ MPIT+ Sbjct: 248 LIVTCTSVHSRNWRAGNMLGKGENLDEVLEKMGMVVEGVRTAKAVHGWAKKLDIDMPITE 307 Query: 300 QIYHVLFQGRTPRSAVEALMGRDRKTEMEA 329 IY +LF+ + R AV+ LMGR++K E E+ Sbjct: 308 SIYAILFENKDAREAVDLLMGREKKIEKES 337 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.316 0.131 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 446,607,425 Number of extensions: 16602384 Number of successful extensions: 48016 Number of sequences better than 1.0e-05: 696 Number of HSP's gapped: 47172 Number of HSP's successfully gapped: 705 Length of query: 340 Length of database: 757,237,578 Length adjustment: 126 Effective length of query: 214 Effective length of database: 415,135,104 Effective search space: 88838912256 Effective search space used: 88838912256 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 126 (53.1 bits)