BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0256.0039.p (317 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value sp|P55785|HEP2_BACST Heptaprenyl diphosphate synthase component ... 349 5e-95 ref|NP_242519.1| heptaprenyl diphosphate synthase component II (... 341 1e-92 ref|NP_390155.1| heptaprenyl diphosphate synthase component II [... 327 3e-88 gb|AAB41847.1| spore germination protein C3 326 5e-88 ref|NP_692709.1| heptaprenyl diphosphate synthase component II [... 324 2e-87 >sp|P55785|HEP2_BACST Heptaprenyl diphosphate synthase component II (HEPPP synthase subunit 2) dbj|BAA08725.1| component II of heptaprenyl diphosphate synthase [Geobacillus stearothermophilus] Length = 320 Score = 349 bits (895), Expect = 5e-95, Method: Compositional matrix adjust. Identities = 172/322 (53%), Positives = 235/322 (72%), Gaps = 12/322 (3%) Query: 1 MFGLLNKDMDQIEKELYRSVQGDDDLLTETSLHLLKAGGKRLRPVFVLMGGKFGQYDLEK 60 M+ L+ D+ +E+EL R+VQ + L E +LHLL+AGGKR+RPVFVL+ +FGQYDLE+ Sbjct: 6 MYSFLSDDLAAVEEELERAVQSEYGPLGEAALHLLQAGGKRIRPVFVLLAARFGQYDLER 65 Query: 61 LKRVAIPLELIHSASLVHDDVIDDAELRRGELTVKAKWGDKIAMYTGDYIYAKALVMTSE 120 +K VA+ LELIH ASLVHDDVIDDA+LRRG T+KAKW ++ AMYTGDY++A++L +E Sbjct: 66 MKHVAVALELIHMASLVHDDVIDDADLRRGRPTIKAKWSNRFAMYTGDYLFARSLERMAE 125 Query: 121 LGNPRIHQILSKAMVEMSIGEMEQIRDFFNSEQSVRHYLRRIRRKTALLIAISCQLGALA 180 LGNPR HQ+L+K +VE+ GE+EQI+D + +Q +R YLRRIRRKTALLIA SCQLGALA Sbjct: 126 LGNPRAHQVLAKTIVEVCRGEIEQIKDKYRFDQPLRTYLRRIRRKTALLIAASCQLGALA 185 Query: 181 ADAEPNTARLLYDYGYNVGMAFQIRDDLLDLSGTEKQIGKPPGSDMRQGNITLPVIYSLE 240 A A + LY +G+ VGM+FQI DD+LD +GTE+Q+GKP GSD+ QGN+TLPV+Y+L Sbjct: 186 AGAPEPIVKRLYWFGHYVGMSFQITDDILDFTGTEEQLGKPAGSDLLQGNVTLPVLYALS 245 Query: 241 DQRLRSALL-----TELASIREGNSGVGRAIDLILSGEGIARAEELASRYIAKALEALDQ 295 D+R+++A+ T++A + S + R + I R+ L+ RY+ KAL LD Sbjct: 246 DERVKAAIAAVGPETDVAEMAAVISAIKRT-------DAIERSYALSDRYLDKALHLLDG 298 Query: 296 LPNNRTKRNLKDIAFFVTGRSY 317 LP N + L+D+A ++ R Y Sbjct: 299 LPMNEARGLLRDLALYIGKRDY 320 >ref|NP_242519.1| heptaprenyl diphosphate synthase component II (spore germination protein C3) [Bacillus halodurans] pir||E83856 heptaprenyl diphosphate synthase component II (spore germination protein C3) gerCC [imported] - Bacillus halodurans (strain C-125) dbj|BAB05372.1| heptaprenyl diphosphate synthase component II (spore germination protein C3) [Bacillus halodurans] Length = 323 Score = 341 bits (875), Expect = 1e-92, Method: Compositional matrix adjust. Identities = 166/318 (52%), Positives = 229/318 (72%), Gaps = 1/318 (0%) Query: 1 MFGLLNKDMDQIEKELYRSVQGDDDLLTETSLHLLKAGGKRLRPVFVLMGGKFGQYDLEK 60 ++ +D+ IE+EL +VQ D+L + S+HLLKAGGKR+RPVFVL+ GKFG YD+ Sbjct: 6 IYAHFQEDIFTIERELEEAVQARHDILRKASVHLLKAGGKRIRPVFVLLAGKFGTYDVNV 65 Query: 61 LKRVAIPLELIHSASLVHDDVIDDAELRRGELTVKAKWGDKIAMYTGDYIYAKALVMTSE 120 LK +A+PLELIH ASLVHDDVIDDA+LRRG+ T+K++W ++IAMYTGDY++AKA+ + Sbjct: 66 LKHIAVPLELIHMASLVHDDVIDDADLRRGKKTIKSQWDNRIAMYTGDYMFAKAVQTATY 125 Query: 121 LGNPRIHQILSKAMVEMSIGEMEQIRDFFNSEQSVRHYLRRIRRKTALLIAISCQLGALA 180 +P IHQI+S A+ EM +GE+EQIRD +N Q +R Y RRI+RKTALLIA+SCQLGALA Sbjct: 126 FDDPNIHQIISNAINEMCLGEVEQIRDQYNPNQHLRIYFRRIKRKTALLIAVSCQLGALA 185 Query: 181 ADAEPNTARLLYDYGYNVGMAFQIRDDLLDLSGTEKQIGKPPGSDMRQGNITLPVIYSLE 240 A A+P + LY +GY VGM+FQI DD+LD GTE+Q+GKP GSD+ QGN+TLP +Y+L Sbjct: 186 AKADPAIVQKLYYFGYYVGMSFQITDDVLDFVGTEEQLGKPAGSDLLQGNVTLPALYALH 245 Query: 241 DQRLRSALLTELASIREGNS-GVGRAIDLILSGEGIARAEELASRYIAKALEALDQLPNN 299 L+ ++ + R NS + I I+S GI A ++ Y+ KA+ LD LP++ Sbjct: 246 HDMHAKRLIEQVLADRGNNSVHMEEVIAAIVSSGGIDYALSISDLYLKKAMRMLDHLPDD 305 Query: 300 RTKRNLKDIAFFVTGRSY 317 K+ L++IA ++ R + Sbjct: 306 EPKQFLQEIANYIGRRKF 323 >ref|NP_390155.1| heptaprenyl diphosphate synthase component II [Bacillus subtilis] sp|P31114|HEP2_BACSU Heptaprenyl diphosphate synthase component II (HEPPP synthase subunit 2) (Spore germination protein C3) pir||E69630 heptaprenyl diphosphate synthase component II gerCC - Bacillus subtilis gb|AAA20856.1| GerC3 [Bacillus subtilis] emb|CAB14190.1| heptaprenyl diphosphate synthase component II [Bacillus subtilis subsp. subtilis str. 168] Length = 348 Score = 327 bits (837), Expect = 3e-88, Method: Compositional matrix adjust. Identities = 158/316 (50%), Positives = 229/316 (72%), Gaps = 2/316 (0%) Query: 2 FGLLNKDMDQIEKELYRSVQGDDDLLTETSLHLLKAGGKRLRPVFVLMGGKFGQYDLEKL 61 + LN D+D IE+EL ++V+ D LL+E LHLL+AGGKR+RPVFVL+ G FG YD+ K+ Sbjct: 35 YSFLNDDIDVIERELEQTVRSDYPLLSEAGLHLLQAGGKRIRPVFVLLSGMFGDYDINKI 94 Query: 62 KRVAIPLELIHSASLVHDDVIDDAELRRGELTVKAKWGDKIAMYTGDYIYAKALVMTSEL 121 K VA+ LE+IH ASLVHDDVIDDAELRRG+ T+KAKW ++IAMYTGDY+ A +L M + + Sbjct: 95 KYVAVTLEMIHMASLVHDDVIDDAELRRGKPTIKAKWDNRIAMYTGDYMLAGSLEMMTRI 154 Query: 122 GNPRIHQILSKAMVEMSIGEMEQIRDFFNSEQSVRHYLRRIRRKTALLIAISCQLGALAA 181 P+ H+ILS+ +VE+ +GE+EQI+D +N EQ++R YLRRI+RKTALLIA+SCQLGA+A+ Sbjct: 155 NEPKAHRILSQTIVEVCLGEIEQIKDKYNMEQNLRTYLRRIKRKTALLIAVSCQLGAIAS 214 Query: 182 DAEPNTARLLYDYGYNVGMAFQIRDDLLDLSGTEKQIGKPPGSDMRQGNITLPVIYSLED 241 A+ + LY +GY VGM++QI DD+LD + TE+++GKP G D+ QGN+TLPV+Y+L++ Sbjct: 215 GADEKIHKALYWFGYYVGMSYQIIDDILDFTSTEEELGKPVGGDLLQGNVTLPVLYALKN 274 Query: 242 QRLRSALLTELASIREGNSGVGRAIDLILSGEGIARAEELASRYIAKALEALDQLPNNRT 301 L++ L +L + + I+ I + I + ++ Y+ KA + L+ LP R Sbjct: 275 PALKNQL--KLINSETTQEQLEPIIEEIKKTDAIEASMAVSEMYLQKAFQKLNTLPRGRA 332 Query: 302 KRNLKDIAFFVTGRSY 317 + +L IA ++ R + Sbjct: 333 RSSLAAIAKYIGKRKF 348 >gb|AAB41847.1| spore germination protein C3 Length = 288 Score = 326 bits (835), Expect = 5e-88, Method: Compositional matrix adjust. Identities = 150/248 (60%), Positives = 194/248 (78%) Query: 5 LNKDMDQIEKELYRSVQGDDDLLTETSLHLLKAGGKRLRPVFVLMGGKFGQYDLEKLKRV 64 D+ IE+EL SVQ D+L S HLLKAGGKR+RPVFVL+ KFG YD+ LK + Sbjct: 17 FQSDITIIEQELEASVQARHDVLKNASTHLLKAGGKRIRPVFVLLAAKFGTYDIHVLKNI 76 Query: 65 AIPLELIHSASLVHDDVIDDAELRRGELTVKAKWGDKIAMYTGDYIYAKALVMTSELGNP 124 A+PLEL+H ASLVHDDVIDDAELRRG+ TVK++W +K+AMYTG+YI+AKA+ ++ +P Sbjct: 77 AVPLELVHMASLVHDDVIDDAELRRGKRTVKSQWDNKVAMYTGNYIFAKAVEASAYFDHP 136 Query: 125 RIHQILSKAMVEMSIGEMEQIRDFFNSEQSVRHYLRRIRRKTALLIAISCQLGALAADAE 184 R+HQ+LS +VEM IGE+EQIRD +N Q++R YLRRI+RKTALLIA+SC+LGALAADA+ Sbjct: 137 RVHQVLSNTLVEMCIGEVEQIRDQYNWGQNLRVYLRRIKRKTALLIAVSCELGALAADAD 196 Query: 185 PNTARLLYDYGYNVGMAFQIRDDLLDLSGTEKQIGKPPGSDMRQGNITLPVIYSLEDQRL 244 P + LY++GYNVGM+FQI DD+LD GTEKQ+GKP G D+RQGN+TLP +Y++ Sbjct: 197 PVIQKHLYNFGYNVGMSFQITDDILDFVGTEKQLGKPAGGDLRQGNVTLPALYAMHHDEE 256 Query: 245 RSALLTEL 252 S L+ + Sbjct: 257 ISHLIKNM 264 >ref|NP_692709.1| heptaprenyl diphosphate synthase component II [Oceanobacillus iheyensis HTE831] dbj|BAC13744.1| heptaprenyl diphosphate synthase component II (spore germination protein C3) [Oceanobacillus iheyensis HTE831] Length = 323 Score = 324 bits (831), Expect = 2e-87, Method: Compositional matrix adjust. Identities = 158/318 (49%), Positives = 224/318 (70%), Gaps = 5/318 (1%) Query: 1 MFGLLNKDMDQIEKELYRSVQGDDDLLTETSLHLLKAGGKRLRPVFVLMGGKFGQYDLEK 60 ++G L KDM+ IE L S+Q + +L + S LL AGGKR+RPVFVL+ + G YDL K Sbjct: 6 IYGFLKKDMESIESALQDSIQANHPVLRDASTELLHAGGKRIRPVFVLLSSQLGNYDLSK 65 Query: 61 LKRVAIPLELIHSASLVHDDVIDDAELRRGELTVKAKWGDKIAMYTGDYIYAKALVMTSE 120 +K+VA+ LELIH A+LVHDDVID+AELRRG+ T + +G+++AMYTGDYI A+AL +E Sbjct: 66 IKKVAVTLELIHMATLVHDDVIDEAELRRGKPTTASIYGNRVAMYTGDYILARALEEITE 125 Query: 121 LGNPRIHQILSKAMVEMSIGEMEQIRDFFNSEQSVRHYLRRIRRKTALLIAISCQLGALA 180 + +PR+HQ+LSK +VE+SIGE+EQI FN EQ+ R YLRRI+RKTALLIA SC+LGA+ Sbjct: 126 IPDPRVHQLLSKTLVEVSIGEIEQIEYKFNWEQNFRDYLRRIKRKTALLIASSCKLGAIV 185 Query: 181 ADAEPNTARLLYDYGYNVGMAFQIRDDLLDLSGTEKQIGKPPGSDMRQGNITLPVIYSLE 240 LY YGY +GM++QI DD+LD + T K++GKP G+D+ QGNITLPV+Y++E Sbjct: 186 GGLNDYQTNKLYQYGYYIGMSYQIIDDILDFTSTSKELGKPAGNDLLQGNITLPVLYAME 245 Query: 241 DQRLRSALLTELASIREGNSGVGRAIDLILS---GEGIARAEELASRYIAKALEALDQLP 297 D+ + +L + ++G+ LI + I R+ ++ Y++KAL ALD++P Sbjct: 246 DKTFKQSLFNVFS--KDGHVTTNEMKTLIQHLKRTDAIKRSYAVSDMYLSKALTALDEIP 303 Query: 298 NNRTKRNLKDIAFFVTGR 315 +R K+ L+DIA ++ R Sbjct: 304 ASRAKKTLQDIATYIGKR 321 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.320 0.138 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 445,590,767 Number of extensions: 17859661 Number of successful extensions: 51228 Number of sequences better than 1.0e-05: 1438 Number of HSP's gapped: 49527 Number of HSP's successfully gapped: 1445 Length of query: 317 Length of database: 757,237,578 Length adjustment: 125 Effective length of query: 192 Effective length of database: 417,850,203 Effective search space: 80227238976 Effective search space used: 80227238976 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 126 (53.1 bits)