BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0256.0031.p (509 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00062051.1| hypothetical protein [Clostridium thermocellu... 483 e-135 ref|ZP_00079750.1| COG0147: Anthranilate/para-aminobenzoate synt... 429 e-119 ref|NP_953429.1| anthranilate synthase component I [Geobacter su... 424 e-117 ref|NP_868122.1| anthranilate synthase component I [Pirellula sp... 406 e-112 ref|NP_623183.1| Anthranilate/para-aminobenzoate synthases compo... 400 e-110 >ref|ZP_00062051.1| hypothetical protein [Clostridium thermocellum ATCC 27405] sp|P14953|TRPE_CLOTM Anthranilate synthase component I pir||JX0065 anthranilate synthase (EC 4.1.3.27) component I - Clostridium thermocellum dbj|BAA00300.1| trpE [Clostridium thermocellum] prf||1505348A anthranilate synthase I Length = 494 Score = 483 bits (1242), Expect = e-135, Method: Compositional matrix adjust. Identities = 247/485 (50%), Positives = 321/485 (66%), Gaps = 9/485 (1%) Query: 4 LSREYNLIPVVTRLLADMETPIRLFQRFAGQDRAFLLESVEGGIQWARYSFIGSDPFLMV 63 ++++YN+IPV + ADMETPI LF+RF FLLESVEGG +WARYS IG +PFL+V Sbjct: 12 MAKDYNIIPVTMEVYADMETPISLFKRFEESSCCFLLESVEGGEKWARYSIIGKNPFLVV 71 Query: 64 -SXXXXXXXXXXXXXXRRLSGKPVEELKALLRSYRSPKLDGMPPFTGGAIGFFGYDLLQY 122 S R + G PVE +K ++ ++ L +P F GGA+G+FGYDL+++ Sbjct: 72 ESYKNKTIIRERNGSQREVEGNPVEIIKGIMGKFKGANLPNLPRFNGGAVGYFGYDLIRH 131 Query: 123 YEKLSAHAVDDLNMDDIRFMFCDRIIVFDHVKQQILLVGNLHIKDGDTDSDIRAGYEELS 182 YE L DD+ + + FMF D ++V+DH+KQ+I ++ NLH+ + +I Y Sbjct: 132 YENLPNVPEDDMGLPECHFMFTDEVLVYDHLKQKIHIIVNLHV-----NGNIERAYISAV 186 Query: 183 RKLENMAEEL--QKEGPKENVNRRSIPQDIELGEIHSNLTREQYISNVEQAKEYIRAGDI 240 +++ + E+ + +N + EL + SN+++E + NV +AK+YIR GDI Sbjct: 187 DRIKTIHREILDTRWKTADNSVLSYNKKKNELA-VTSNISKEDFCRNVLKAKQYIRDGDI 245 Query: 241 FQVVLSQRLHIETEVSPLHVYRMLRTLNPSPYMYYLKMDEEIIVGTSPEALVKVDGGRVE 300 FQVVLSQRL +ET +P ++YR LR +NPSPYMYYLK I+G+SPE LV+V+ G VE Sbjct: 246 FQVVLSQRLCVETNENPFNIYRALRVINPSPYMYYLKFGGYRIIGSSPEMLVRVENGIVE 305 Query: 301 TRPIAGTRPRGENXXXXXXXXXXXXXXXXXXXXHLMLVDLGRNDLGRVSKFGTVKCDSFM 360 T PIAGTR RG H+MLVDLGRND+GRVSKFGTV + M Sbjct: 306 TCPIAGTRKRGRTKEEDEALEKELLSDEKEIAEHVMLVDLGRNDIGRVSKFGTVAVKNLM 365 Query: 361 EIERYSHVMHIVSNVSGTLDEGKDFFDAFLSCLPAGTVSGAPKLRAMEIIAELEREARGA 420 IERYSHVMH+V+NV G + E K FDA +S LPAGT+SGAPK+RAMEII ELE RG Sbjct: 366 HIERYSHVMHVVTNVQGEIREDKTPFDALMSILPAGTLSGAPKVRAMEIIDELETVKRGP 425 Query: 421 YAGAIGYLGFSGNMDSCITIRTIIFRKGRAYVQAGAGIVWDSVPEKEYEETVNKAKAMLK 480 Y GAIGYL F+GN+DSCITIRTII + G+AYVQAGAGIV DSVPE+EYEE NKA A+LK Sbjct: 426 YGGAIGYLSFNGNLDSCITIRTIILKDGKAYVQAGAGIVADSVPEREYEECYNKAMALLK 485 Query: 481 AIRMA 485 AI A Sbjct: 486 AIEEA 490 >ref|ZP_00079750.1| COG0147: Anthranilate/para-aminobenzoate synthases component I [Geobacter metallireducens] Length = 491 Score = 429 bits (1104), Expect = e-119, Method: Compositional matrix adjust. Identities = 223/485 (45%), Positives = 299/485 (61%), Gaps = 9/485 (1%) Query: 3 SLSREYNLIPVVTRLLADMETPIRLFQRFAGQDRAFLLESVEGGIQWARYSFIGSDPFLM 62 SL+ + NLIPV ++ADM+TP+ F++ +FLLES+EGG +W RYSF+GS+P + Sbjct: 11 SLASQGNLIPVCREIMADMDTPVSAFRKIDDGSYSFLLESIEGGEKWGRYSFLGSNPATV 70 Query: 63 VSXXXXXXXXXXXXXXRRL-SGKPVEELKALLRSYRSPKLDGMPPFTGGAIGFFGYDLLQ 121 + R + S P+ ++ L + ++ G+P F GGA+G+ GYD+++ Sbjct: 71 IRTRGTTVEIVTNGETRAVTSDDPLGFVREYLSRFHPVEIPGLPRFFGGAVGYLGYDMVR 130 Query: 122 YYEKLSAHAVDDLNMDDIRFMFCDRIIVFDHVKQQILLVGNLHIKDGDTDSDIRAGYEEL 181 ++E+L + D F+ D I++FD ++Q+I +V N H++DG A Y E Sbjct: 131 HFERLPTEKPAVIGAWDSCFVITDTILIFDTMRQKITVVSNAHLEDGQAPE---AAYAEA 187 Query: 182 SRKLENMAEELQKEGPKENVNRRSIPQDIELGEIHSNLTREQYISNVEQAKEYIRAGDIF 241 +++ + +L+ P + SN+ RE + + VE+ KEY+RAGDI Sbjct: 188 IARVDALIAKLRAPLPAMPAT-----PAVRTVSFSSNVPRENFEAAVEKCKEYVRAGDII 242 Query: 242 QVVLSQRLHIETEVSPLHVYRMLRTLNPSPYMYYLKMDEEIIVGTSPEALVKVDGGRVET 301 QVVLSQR V PL +YR+LRTLNPSPYM++L+MD+ ++VG SPE +V+ +G RVE Sbjct: 243 QVVLSQRFSGALTVEPLAIYRVLRTLNPSPYMFFLRMDDTLVVGASPEVMVRKEGDRVEL 302 Query: 302 RPIAGTRPRGENXXXXXXXXXXXXXXXXXXXXHLMLVDLGRNDLGRVSKFGTVKCDSFME 361 RPIAGTRPRG H+MLVDLGRNDLGRV + GTVK M Sbjct: 303 RPIAGTRPRGATPEEDQQLAEELLADPKERAEHVMLVDLGRNDLGRVCRTGTVKVSELMV 362 Query: 362 IERYSHVMHIVSNVSGTLDEGKDFFDAFLSCLPAGTVSGAPKLRAMEIIAELEREARGAY 421 IERYSHVMHIVSNV G L EGKD FD + PAGT+SGAPK+RAMEII ELE R Y Sbjct: 363 IERYSHVMHIVSNVEGELVEGKDAFDVVRATFPAGTLSGAPKVRAMEIIDELEPVRREVY 422 Query: 422 AGAIGYLGFSGNMDSCITIRTIIFRKGRAYVQAGAGIVWDSVPEKEYEETVNKAKAMLKA 481 GA+GY FSGNMD I IRT++ R G ++QAGAGIV DS P EY+ETVNKA A +KA Sbjct: 423 GGAVGYFSFSGNMDLAIAIRTLVIRDGMVHLQAGAGIVADSDPAAEYQETVNKAMAAVKA 482 Query: 482 IRMAE 486 I AE Sbjct: 483 IETAE 487 >ref|NP_953429.1| anthranilate synthase component I [Geobacter sulfurreducens PCA] gb|AAR35756.1| anthranilate synthase component I [Geobacter sulfurreducens PCA] Length = 491 Score = 424 bits (1089), Expect = e-117, Method: Compositional matrix adjust. Identities = 220/485 (45%), Positives = 300/485 (61%), Gaps = 9/485 (1%) Query: 3 SLSREYNLIPVVTRLLADMETPIRLFQRFAGQDRAFLLESVEGGIQWARYSFIGSDPFLM 62 +L NLIPV ++ADM+TP+ F++ AFLLES+EGG +WARY+F+G+ P + Sbjct: 11 ALGARGNLIPVCREIMADMDTPVSAFRKLDDGRYAFLLESIEGGEKWARYTFLGASPSTV 70 Query: 63 VSXXXXXXXXXXXXXXRRLSGK-PVEELKALLRSYRSPKLDGMPPFTGGAIGFFGYDLLQ 121 + R ++ P+ ++ L +R ++ G+P F GGA+G+ GYD+++ Sbjct: 71 IRSRGTTVEIITNGETRSVTTDDPLGFVRDFLARFRPVEIPGLPRFFGGAVGYLGYDMVR 130 Query: 122 YYEKLSAHAVDDLNMDDIRFMFCDRIIVFDHVKQQILLVGNLHIKDGDTDSDIRAGYEEL 181 +E+L + D F+ D I++FD+++Q+I +V N H+ +G + + A Y + Sbjct: 131 QFERLPTDKPAVIGAWDSCFLITDTIVIFDNMRQKITVVSNAHLDEGVS---VEAAYADA 187 Query: 182 SRKLENMAEELQKEGPKENVNRRSIPQDIELGEIHSNLTREQYISNVEQAKEYIRAGDIF 241 +++ + L K + + SN+TRE + VE+AKEY+RAGDI Sbjct: 188 VARIDGIIARL-----KAPLPAQPAAAAARKVSFSSNITREAFEDAVERAKEYVRAGDII 242 Query: 242 QVVLSQRLHIETEVSPLHVYRMLRTLNPSPYMYYLKMDEEIIVGTSPEALVKVDGGRVET 301 QVVLSQR E V PL +YR+LRTLNPSPYM++L++D+ ++VG SPE +V+ +G RVE Sbjct: 243 QVVLSQRFSGELTVDPLDIYRVLRTLNPSPYMFFLRLDDTLVVGASPEVMVRREGNRVEL 302 Query: 302 RPIAGTRPRGENXXXXXXXXXXXXXXXXXXXXHLMLVDLGRNDLGRVSKFGTVKCDSFME 361 RPIAGTRPRG H+MLVDLGRNDLGRV + GTVK M Sbjct: 303 RPIAGTRPRGATPEQDEQLAEELLADPKERAEHVMLVDLGRNDLGRVCRTGTVKVSELMV 362 Query: 362 IERYSHVMHIVSNVSGTLDEGKDFFDAFLSCLPAGTVSGAPKLRAMEIIAELEREARGAY 421 IERYSHVMHIVSNV G L EG+D FD + PAGT+SGAPK+RAMEII ELE R Y Sbjct: 363 IERYSHVMHIVSNVQGELAEGRDAFDVVRATFPAGTLSGAPKVRAMEIIDELEPVRREVY 422 Query: 422 AGAIGYLGFSGNMDSCITIRTIIFRKGRAYVQAGAGIVWDSVPEKEYEETVNKAKAMLKA 481 GA+GY FSG MD I IRT++ R G ++QAGAGIV DS P EY+ETVNKA A++KA Sbjct: 423 GGAVGYFSFSGTMDLAIAIRTLVIRDGVVHLQAGAGIVADSDPASEYQETVNKAMAVVKA 482 Query: 482 IRMAE 486 I AE Sbjct: 483 IETAE 487 >ref|NP_868122.1| anthranilate synthase component I [Pirellula sp. 1] emb|CAD75674.1| anthranilate synthase component I [Pirellula sp.] Length = 547 Score = 406 bits (1043), Expect = e-112, Method: Compositional matrix adjust. Identities = 215/494 (43%), Positives = 292/494 (59%), Gaps = 23/494 (4%) Query: 3 SLSREYNLIPVVTRLLADMETPIRLFQRFAGQDRAFLLESVEGGIQWARYSFIGSDPFLM 62 SL+R ++ +PV RLL+D TP+ F AFL+ESV GG + R+SF+GS P Sbjct: 52 SLARRFDFVPVYRRLLSDTLTPVTAFMHLDDGGPAFLIESVIGGEKVGRFSFLGSRPLAR 111 Query: 63 VSXXXXXXXXXXXXXXRRLSGKPVEELKALLRSY--RSPKLDGMPPFTGGAIGFFGYDLL 120 + + + + L A S+ R+ LDG+PPF GGAIG+ GYD++ Sbjct: 112 FTAKGNTVRRTNMSTGEVVESQCDDPLNAFRESFQDRTATLDGLPPFIGGAIGYAGYDVV 171 Query: 121 QYYEKLSAHAVDDLNMDDIRFMFCDRIIVFDHVKQQILLVGNLHIKDGDTDSDIRAGYEE 180 +Y E L DD N+ D+ F F + VFDHV + I +V + D ++D A Sbjct: 172 RYVENLPDTTEDDRNLPDLNFAFYHTLCVFDHVDKTITVVSLADCRGIDEETDSTA---- 227 Query: 181 LSRKLENMAEELQKEGPKENVNR-RSIPQDIELGEI-----------HSNLTREQYISNV 228 +S + AEE+ + + R + P D+ + EI SN TRE + V Sbjct: 228 ISEARQAAAEEVD-----QTIKRLMTSPPDLRVDEIIEEDQRQPLPVESNFTRESFCDAV 282 Query: 229 EQAKEYIRAGDIFQVVLSQRLHIETEVSPLHVYRMLRTLNPSPYMYYLKMDEEIIVGTSP 288 EYIRAGDIFQVV SQRL ++++V P VYR LR +NPSP+M++++ E ++VG SP Sbjct: 283 RHCVEYIRAGDIFQVVPSQRLTVKSDVDPFAVYRSLRVVNPSPFMFFVRNPECVLVGCSP 342 Query: 289 EALVKVDGGRVETRPIAGTRPRGENXXXXXXXXXXXXXXXXXXXXHLMLVDLGRNDLGRV 348 E + +V V RP+AGTRPRG H+MLVDLGRND+GR+ Sbjct: 343 EIMCRVKDREVTVRPLAGTRPRGATEKEDKALEKDLLADPKERAEHVMLVDLGRNDIGRI 402 Query: 349 SKFGTVKCDSFMEIERYSHVMHIVSNVSGTLDEGKDFFDAFLSCLPAGTVSGAPKLRAME 408 ++FGTV M IERYSHVMHI S V G L EG D FDA SCLPAGTVSGAPK+RAM+ Sbjct: 403 AEFGTVDLTEIMVIERYSHVMHISSEVRGKLREGLDAFDALKSCLPAGTVSGAPKVRAMQ 462 Query: 409 IIAELEREARGAYAGAIGYLGFSGNMDSCITIRTIIFRKGRAYVQAGAGIVWDSVPEKEY 468 +I E+E RG Y GA+GY+ + GNMD+C+ +RTI+ + G +VQAG G+V DS P+ EY Sbjct: 463 VIDEIEPHRRGPYGGAVGYIDYRGNMDTCLALRTIVHQNGAYHVQAGCGVVADSNPDSEY 522 Query: 469 EETVNKAKAMLKAI 482 EET+NKA+A++ AI Sbjct: 523 EETLNKARALIAAI 536 >ref|NP_623183.1| Anthranilate/para-aminobenzoate synthases component I [Thermoanaerobacter tengcongensis] gb|AAM24787.1| Anthranilate/para-aminobenzoate synthases component I [Thermoanaerobacter tengcongensis] Length = 476 Score = 400 bits (1027), Expect = e-110, Method: Compositional matrix adjust. Identities = 213/471 (45%), Positives = 288/471 (61%), Gaps = 20/471 (4%) Query: 11 IPVVTRLLADMETPIRLFQRFAGQDRAFLLESVEGGIQWARYSFIGSDPFLMVSXXXXXX 70 PV + D TPI +F +G+ + FLLES GG W RYSFIG DP+L V Sbjct: 19 FPVYEEINGDEITPINVFYSLSGKHK-FLLESANGGSHWGRYSFIGKDPYLSVLSYGRDI 77 Query: 71 XXXXXXXXRRLSGKPVEELKALLRSYRSPKLDGMPPFTGGAIGFFGYDLLQYYEKLSAHA 130 G +EE+K L+ +P L PFTGGA+G+ YD ++ YEKL Sbjct: 78 KIITTEEESNKRGLALEEVKNFLQINYNP-LGLNIPFTGGAVGYVSYDAIRLYEKLPEKN 136 Query: 131 VDDLNMDDIRFMFCDRIIVFDHVKQQILLVGNLHIKDGDTDSDIRAGYEELSRKLENMAE 190 D++N+ D+ FMF I +DH K ++ +V N+ + DT+ YEE+ +K+ + Sbjct: 137 PDEINIPDVYFMFYKSFICYDHFKHEVYIVYNVFANE-DTE------YEEVLQKINELLN 189 Query: 191 ELQKEGPKENVNRRSIPQDIELGE---IHSNLTREQYISNVEQAKEYIRAGDIFQVVLSQ 247 E++ N+ D+ L E I N T+E++ VE+AK+YI GDIFQVVLSQ Sbjct: 190 EIKS-------NKIEF-HDLSLQEEKVITYNFTKEEFCQIVERAKKYIEKGDIFQVVLSQ 241 Query: 248 RLHIETEVSPLHVYRMLRTLNPSPYMYYLKMDEEIIVGTSPEALVKVDGGRVETRPIAGT 307 RL +T P +YR LR+ NPSPY++Y+ + ++G+SPE+LV+V +V T PIAGT Sbjct: 242 RLKAKTNSDPFEIYRRLRSKNPSPYLFYIDFGDFQLLGSSPESLVRVFQDKVTTNPIAGT 301 Query: 308 RPRGENXXXXXXXXXXXXXXXXXXXXHLMLVDLGRNDLGRVSKFGTVKCDSFMEIERYSH 367 R RG++ H+MLVDLGRND+G+VS+FG+VK + FME++ YSH Sbjct: 302 RRRGKDEKEDLKNKEELLKDEKERAEHVMLVDLGRNDIGKVSEFGSVKVERFMEVDFYSH 361 Query: 368 VMHIVSNVSGTLDEGKDFFDAFLSCLPAGTVSGAPKLRAMEIIAELEREARGAYAGAIGY 427 VMHIVS VSG L +G FDA ++CLPAGTVSGAPK+RAMEII ELE R YAGA+GY Sbjct: 362 VMHIVSTVSGKLKKGFTAFDALIACLPAGTVSGAPKIRAMEIIEELENVKRSFYAGAVGY 421 Query: 428 LGFSGNMDSCITIRTIIFRKGRAYVQAGAGIVWDSVPEKEYEETVNKAKAM 478 ++GNMD CI IRTI+ ++ AY QAGAGIV+DS+PE EY E +NK + Sbjct: 422 FSYNGNMDMCIAIRTILIKENTAYFQAGAGIVYDSIPEMEYYEILNKVMVL 472 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.320 0.138 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 702,553,419 Number of extensions: 28668237 Number of successful extensions: 75934 Number of sequences better than 1.0e-05: 1423 Number of HSP's gapped: 73419 Number of HSP's successfully gapped: 1430 Length of query: 509 Length of database: 757,237,578 Length adjustment: 130 Effective length of query: 379 Effective length of database: 404,274,708 Effective search space: 153220114332 Effective search space used: 153220114332 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 128 (53.9 bits)