BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0256.0003.p (140 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_242547.1| methylglyoxal synthase [Bacillus halodurans] >g... 198 4e-50 ref|NP_390129.1| alternate gene name: jojF~similar to hypothetic... 187 9e-47 ref|NP_831312.1| Methylglyoxal synthase [Bacillus cereus ATCC 14... 185 2e-46 ref|NP_977983.1| methylglyoxal synthase [Bacillus cereus ATCC 10... 185 3e-46 ref|NP_655434.1| MGS, MGS-like domain [Bacillus anthracis A2012]... 185 3e-46 >ref|NP_242547.1| methylglyoxal synthase [Bacillus halodurans] sp|Q9KC92|MGSA_BACHD Methylglyoxal synthase (MGS) pir||A83860 methylglyoxal synthase mgsA [imported] - Bacillus halodurans (strain C-125) dbj|BAB05400.1| methylglyoxal synthase [Bacillus halodurans] Length = 138 Score = 198 bits (503), Expect = 4e-50, Method: Compositional matrix adjust. Identities = 89/131 (67%), Positives = 108/131 (82%) Query: 2 LKIAFIAHDRKKDEMVNFVTAYEHVFVGHQLYSTGTTGQRIMEVTKLSIHRYMSGPLGGD 61 ++IA IAHD+KKDEMV F AY+ V H+LY+TGTTG RIME +L + R+ SGPLGGD Sbjct: 1 MRIALIAHDKKKDEMVQFTIAYKDVLKDHELYATGTTGMRIMEAAQLPVKRFKSGPLGGD 60 Query: 62 QQIGSMVAMDELDLIIFLRDPLMAQPHEPDITALLRLCDVYGIPVATNIATAEILVKAID 121 QQIG++VA ++ DLIIFLRDPL AQPHEPD+TAL+RLCDV +P+ATNI TAEIL+K + Sbjct: 61 QQIGALVAENKFDLIIFLRDPLTAQPHEPDVTALVRLCDVQRVPLATNIGTAEILIKGLA 120 Query: 122 RGDFAWRELVH 132 RGDF WRELVH Sbjct: 121 RGDFTWRELVH 131 >ref|NP_390129.1| alternate gene name: jojF~similar to hypothetical proteins [Bacillus subtilis] sp|P42980|MGSA_BACSU Methylglyoxal synthase (MGS) pir||E69937 conserved hypothetical protein ypjF - Bacillus subtilis gb|AAA92875.1| unknown gb|AAB38443.1| putative [Bacillus subtilis] emb|CAB14164.1| methylglyoxal synthase [Bacillus subtilis subsp. subtilis str. 168] Length = 137 Score = 187 bits (474), Expect = 9e-47, Method: Compositional matrix adjust. Identities = 84/137 (61%), Positives = 107/137 (78%) Query: 2 LKIAFIAHDRKKDEMVNFVTAYEHVFVGHQLYSTGTTGQRIMEVTKLSIHRYMSGPLGGD 61 +KIA IAHD+KK +MV F TAY + H LY+TGTTG +I E T L I R+ SGPLGGD Sbjct: 1 MKIALIAHDKKKQDMVQFTTAYRDILKNHDLYATGTTGLKIHEATGLQIERFQSGPLGGD 60 Query: 62 QQIGSMVAMDELDLIIFLRDPLMAQPHEPDITALLRLCDVYGIPVATNIATAEILVKAID 121 QQIG+++A + LDL+IFLRDPL AQPHEPD++AL+RLCDVY IP+ATN+ TAEILV+ +D Sbjct: 61 QQIGALIAANALDLVIFLRDPLTAQPHEPDVSALIRLCDVYSIPLATNMGTAEILVRTLD 120 Query: 122 RGDFAWRELVHKYKPGV 138 G F +R+L+ +P V Sbjct: 121 EGVFEFRDLLRGEEPNV 137 >ref|NP_831312.1| Methylglyoxal synthase [Bacillus cereus ATCC 14579] gb|AAP08513.1| Methylglyoxal synthase [Bacillus cereus ATCC 14579] Length = 131 Score = 185 bits (470), Expect = 2e-46, Method: Compositional matrix adjust. Identities = 82/129 (63%), Positives = 109/129 (84%) Query: 2 LKIAFIAHDRKKDEMVNFVTAYEHVFVGHQLYSTGTTGQRIMEVTKLSIHRYMSGPLGGD 61 +KIA IAHD+KK++MV+F AY+ +F H+L++TGTTG RIME T L I RY SGPLGGD Sbjct: 1 MKIALIAHDKKKNDMVSFAYAYKPIFEQHELFATGTTGLRIMEATGLVITRYQSGPLGGD 60 Query: 62 QQIGSMVAMDELDLIIFLRDPLMAQPHEPDITALLRLCDVYGIPVATNIATAEILVKAID 121 Q+IG+M+A ++LD++IF RDPL AQPHEPD+ ALLRLCDVY IP+ATN+A+AE+L+ A++ Sbjct: 61 QEIGAMIAKNDLDMVIFFRDPLTAQPHEPDVNALLRLCDVYAIPLATNMASAEMLMHALE 120 Query: 122 RGDFAWREL 130 RGD +R+L Sbjct: 121 RGDLDYRKL 129 >ref|NP_977983.1| methylglyoxal synthase [Bacillus cereus ATCC 10987] gb|AAS40591.1| methylglyoxal synthase [Bacillus cereus ATCC 10987] Length = 131 Score = 185 bits (469), Expect = 3e-46, Method: Compositional matrix adjust. Identities = 81/129 (62%), Positives = 109/129 (84%) Query: 2 LKIAFIAHDRKKDEMVNFVTAYEHVFVGHQLYSTGTTGQRIMEVTKLSIHRYMSGPLGGD 61 +KIA IAHD+KK++MV+F AY+ +F H+L++TGTTG RIME T L + RY SGPLGGD Sbjct: 1 MKIALIAHDKKKNDMVSFAYAYKPIFEQHELFATGTTGLRIMEATGLVVTRYQSGPLGGD 60 Query: 62 QQIGSMVAMDELDLIIFLRDPLMAQPHEPDITALLRLCDVYGIPVATNIATAEILVKAID 121 Q+IG+M+A ++LD++IF RDPL AQPHEPD+ ALLRLCDVY IP+ATN+A+AE+L+ A++ Sbjct: 61 QEIGAMIAKNDLDMVIFFRDPLTAQPHEPDVNALLRLCDVYAIPLATNMASAEMLMHALE 120 Query: 122 RGDFAWREL 130 RGD +R+L Sbjct: 121 RGDLDYRKL 129 >ref|NP_655434.1| MGS, MGS-like domain [Bacillus anthracis A2012] ref|NP_844006.1| methylglyoxal synthase [Bacillus anthracis str. Ames] gb|AAP25492.1| methylglyoxal synthase [Bacillus anthracis str. Ames] Length = 131 Score = 185 bits (469), Expect = 3e-46, Method: Compositional matrix adjust. Identities = 81/129 (62%), Positives = 109/129 (84%) Query: 2 LKIAFIAHDRKKDEMVNFVTAYEHVFVGHQLYSTGTTGQRIMEVTKLSIHRYMSGPLGGD 61 +KIA IAHD+KK++MV+F AY+ +F H+L++TGTTG RIME T L + RY SGPLGGD Sbjct: 1 MKIALIAHDKKKEDMVSFAYAYKPIFEQHELFATGTTGLRIMEATGLVVTRYQSGPLGGD 60 Query: 62 QQIGSMVAMDELDLIIFLRDPLMAQPHEPDITALLRLCDVYGIPVATNIATAEILVKAID 121 Q+IG+M+A ++LD++IF RDPL AQPHEPD+ ALLRLCDVY IP+ATN+A+AE+L+ A++ Sbjct: 61 QEIGAMIAKNDLDMVIFFRDPLTAQPHEPDVNALLRLCDVYAIPLATNMASAEMLMHALE 120 Query: 122 RGDFAWREL 130 RGD +R+L Sbjct: 121 RGDLDYRKL 129 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.324 0.140 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 198,019,200 Number of extensions: 6921215 Number of successful extensions: 14338 Number of sequences better than 1.0e-05: 77 Number of HSP's gapped: 14316 Number of HSP's successfully gapped: 77 Length of query: 140 Length of database: 757,237,578 Length adjustment: 101 Effective length of query: 39 Effective length of database: 483,012,579 Effective search space: 18837490581 Effective search space used: 18837490581 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 120 (50.8 bits)