BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0255.0005.p (367 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|AAG31622.1| cyclodextrin glucanotransferase [Bacillus circulans] 251 2e-65 pdb|1D7F|A Chain A, Crystal Structure Of Asparagine 233-Replaced... 249 6e-65 pdb|1PAM|A Chain A, Cyclodextrin Glucanotransferase >gi|1942572|... 249 6e-65 gb|AAR32682.1| CGTase [Bacillus sp. I-5] 246 8e-64 sp|P43379|CDGU_BACCI Cyclomaltodextrin glucanotransferase precur... 245 1e-63 >gb|AAG31622.1| cyclodextrin glucanotransferase [Bacillus circulans] Length = 713 Score = 251 bits (640), Expect = 2e-65, Method: Compositional matrix adjust. Identities = 132/333 (39%), Positives = 186/333 (55%), Gaps = 6/333 (1%) Query: 23 GLPVVYYGTEQYMSGGDDPHNRAMMSSFSRETTXXXXVAQLNRLRACNLAIGYGSTKLLY 82 G+P +YYG+EQYMSGG+DP NRA + SFS TT + +L LR N AI YGST+ + Sbjct: 381 GVPAIYYGSEQYMSGGNDPDNRARIPSFSTTTTAYQVIQKLAPLRKSNPAIAYGSTQERW 440 Query: 83 VNSGVLVFERSCKRDVALILINQEDNAQI-LPSLATSLPQGAYSSLLKDIYPDHPAVAGA 141 +N+ V+++ER +VA++ IN+ N + L TSLPQG+Y+ +L I + GA Sbjct: 441 INNDVIIYERKFGNNVAVVAINRNMNTPASITGLVTSLPQGSYNDVLGGILNGNTLTVGA 500 Query: 142 EGDMQNVMLAPRSAFVYSYESDLEKPAAVHVYPKAAAPGSEFIIRGRHLGD-TGCLTVGG 200 G N LAP V+ Y +D P +V P A PG I GR G G + G Sbjct: 501 GGAASNFTLAPGGTAVWQYTTDATAPIIGNVGPMMAKPGVTITIDGRGFGSGKGTVYFGT 560 Query: 201 CE---AAVISWQSGKIVARVPAVPAGVHPVQGLSEGGQKFRLPEELKVWTGMLVTVRLVL 257 A +++W+ +I ++PAVP G++ ++ + G + + +V TG VTVR V+ Sbjct: 561 TAVTGADIVAWEDTQIQVKIPAVPGGIYDIRVANAAGAASNIYDNFEVLTGDQVTVRFVI 620 Query: 258 KNVHTDFDTRVYVTGNVYELGAWQAERAAGPFYNQIVYRYPTWYCDISLPADSRIEFKIM 317 N T V++TGNV ELG W A GP YNQ+VY+YPTWY D+S+PA IEFK + Sbjct: 621 NNATTALGQNVFLTGNVSELGNWDPNNAIGPMYNQVVYQYPTWYYDVSVPAGQTIEFKFL 680 Query: 318 LRSGSGMMRLEQGEAHCFHTPAEGVSEIIAEWQ 350 + GS + E G F TP G + + WQ Sbjct: 681 KKQGS-TVTWEGGANRTFTTPTSGTATMNVNWQ 712 >pdb|1D7F|A Chain A, Crystal Structure Of Asparagine 233-Replaced Cyclodextrin Glucanotransferase From Alkalophilic Bacillus Sp. 1011 Determined At 1.9 A Resolution pdb|1D7F|B Chain B, Crystal Structure Of Asparagine 233-Replaced Cyclodextrin Glucanotransferase From Alkalophilic Bacillus Sp. 1011 Determined At 1.9 A Resolution pdb|1DED|A Chain A, Crystal Structure Of Alkalophilic Asparagine 233-Replaced Cyclodextrin Glucanotransferase Complexed With An Inhibitor Acarbose, At 2.0 A Resolution pdb|1DED|B Chain B, Crystal Structure Of Alkalophilic Asparagine 233-Replaced Cyclodextrin Glucanotransferase Complexed With An Inhibitor Acarbose, At 2.0 A Resolution Length = 686 Score = 249 bits (637), Expect = 6e-65, Method: Compositional matrix adjust. Identities = 131/333 (39%), Positives = 185/333 (55%), Gaps = 6/333 (1%) Query: 23 GLPVVYYGTEQYMSGGDDPHNRAMMSSFSRETTXXXXVAQLNRLRACNLAIGYGSTKLLY 82 G+P +YYG+EQYMSGG+DP NRA + SFS TT + +L LR N AI YGST + Sbjct: 354 GVPAIYYGSEQYMSGGNDPDNRARLPSFSTTTTAYQVIQKLAPLRKSNPAIAYGSTHERW 413 Query: 83 VNSGVLVFERSCKRDVALILINQEDNAQI-LPSLATSLPQGAYSSLLKDIYPDHPAVAGA 141 +N+ V+++ER +VA++ IN+ N + L TSLP+G+Y+ +L I + GA Sbjct: 414 INNDVIIYERKFGNNVAVVAINRNMNTPASITGLVTSLPRGSYNDVLGGILNGNTLTVGA 473 Query: 142 EGDMQNVMLAPRSAFVYSYESDLEKPAAVHVYPKAAAPGSEFIIRGRHLGD-TGCLTVGG 200 G N LAP V+ Y +D P +V P A PG I GR G G + G Sbjct: 474 GGAASNFTLAPGGTAVWQYTTDATTPIIGNVGPMMAKPGVTITIDGRGFGSGKGTVYFGT 533 Query: 201 CE---AAVISWQSGKIVARVPAVPAGVHPVQGLSEGGQKFRLPEELKVWTGMLVTVRLVL 257 A +++W+ +I ++PAVP G++ ++ + G + + +V TG VTVR V+ Sbjct: 534 TAVTGADIVAWEDTQIQVKIPAVPGGIYDIRVANAAGAASNIYDNFEVLTGDQVTVRFVI 593 Query: 258 KNVHTDFDTRVYVTGNVYELGAWQAERAAGPFYNQIVYRYPTWYCDISLPADSRIEFKIM 317 N T V++TGNV ELG W A GP YNQ+VY+YPTWY D+S+PA IEFK + Sbjct: 594 NNATTALGQNVFLTGNVSELGNWDPNNAIGPMYNQVVYQYPTWYYDVSVPAGQTIEFKFL 653 Query: 318 LRSGSGMMRLEQGEAHCFHTPAEGVSEIIAEWQ 350 + GS + E G F TP G + + WQ Sbjct: 654 KKQGS-TVTWEGGANRTFTTPTSGTATVNVNWQ 685 >pdb|1PAM|A Chain A, Cyclodextrin Glucanotransferase pdb|1PAM|B Chain B, Cyclodextrin Glucanotransferase pdb|1I75|A Chain A, Crystal Structure Of Cyclodextrin Glucanotransferase From Alkalophilic Bacillus Sp.#1011 Complexed With 1- Deoxynojirimycin pdb|1I75|B Chain B, Crystal Structure Of Cyclodextrin Glucanotransferase From Alkalophilic Bacillus Sp.#1011 Complexed With 1- Deoxynojirimycin Length = 686 Score = 249 bits (637), Expect = 6e-65, Method: Compositional matrix adjust. Identities = 131/333 (39%), Positives = 185/333 (55%), Gaps = 6/333 (1%) Query: 23 GLPVVYYGTEQYMSGGDDPHNRAMMSSFSRETTXXXXVAQLNRLRACNLAIGYGSTKLLY 82 G+P +YYG+EQYMSGG+DP NRA + SFS TT + +L LR N AI YGST + Sbjct: 354 GVPAIYYGSEQYMSGGNDPDNRARLPSFSTTTTAYQVIQKLAPLRKSNPAIAYGSTHERW 413 Query: 83 VNSGVLVFERSCKRDVALILINQEDNAQI-LPSLATSLPQGAYSSLLKDIYPDHPAVAGA 141 +N+ V+++ER +VA++ IN+ N + L TSLP+G+Y+ +L I + GA Sbjct: 414 INNDVIIYERKFGNNVAVVAINRNMNTPASITGLVTSLPRGSYNDVLGGILNGNTLTVGA 473 Query: 142 EGDMQNVMLAPRSAFVYSYESDLEKPAAVHVYPKAAAPGSEFIIRGRHLGD-TGCLTVGG 200 G N LAP V+ Y +D P +V P A PG I GR G G + G Sbjct: 474 GGAASNFTLAPGGTAVWQYTTDATTPIIGNVGPMMAKPGVTITIDGRGFGSGKGTVYFGT 533 Query: 201 CE---AAVISWQSGKIVARVPAVPAGVHPVQGLSEGGQKFRLPEELKVWTGMLVTVRLVL 257 A +++W+ +I ++PAVP G++ ++ + G + + +V TG VTVR V+ Sbjct: 534 TAVTGADIVAWEDTQIQVKIPAVPGGIYDIRVANAAGAASNIYDNFEVLTGDQVTVRFVI 593 Query: 258 KNVHTDFDTRVYVTGNVYELGAWQAERAAGPFYNQIVYRYPTWYCDISLPADSRIEFKIM 317 N T V++TGNV ELG W A GP YNQ+VY+YPTWY D+S+PA IEFK + Sbjct: 594 NNATTALGQNVFLTGNVSELGNWDPNNAIGPMYNQVVYQYPTWYYDVSVPAGQTIEFKFL 653 Query: 318 LRSGSGMMRLEQGEAHCFHTPAEGVSEIIAEWQ 350 + GS + E G F TP G + + WQ Sbjct: 654 KKQGS-TVTWEGGANRTFTTPTSGTATVNVNWQ 685 >gb|AAR32682.1| CGTase [Bacillus sp. I-5] Length = 712 Score = 246 bits (627), Expect = 8e-64, Method: Compositional matrix adjust. Identities = 129/332 (38%), Positives = 183/332 (55%), Gaps = 5/332 (1%) Query: 23 GLPVVYYGTEQYMSGGDDPHNRAMMSSFSRETTXXXXVAQLNRLRACNLAIGYGSTKLLY 82 G+P +YYG+EQYMSGG+DP NRA + SFS TT + +L LR N AI YGST+ + Sbjct: 381 GVPAIYYGSEQYMSGGNDPDNRARIPSFSTTTTAYQVIQKLAPLRKSNPAIAYGSTQERW 440 Query: 83 VNSGVLVFERSCKRDVALILINQEDNAQI-LPSLATSLPQGAYSSLLKDIYPDHPAVAGA 141 +N+ V+++ER +VA++ IN+ N + L TSLPQG+Y+ +L I + GA Sbjct: 441 INNDVIIYERKFGNNVAVVAINRNMNTPASITGLVTSLPQGSYNDVLGGILNGNTLTVGA 500 Query: 142 EGDMQNVMLAPRSAFVYSYESDLEKPAAVHVYPKAAAPGSEFIIRGRHLGDTGCLTVGGC 201 G N LAP V+ Y +D P +V P A PG I GR G + G Sbjct: 501 GGAASNFTLAPGGTAVWQYTTDATAPIIGNVGPMMAKPGVTITIDGRASARQGTVYFGTT 560 Query: 202 E---AAVISWQSGKIVARVPAVPAGVHPVQGLSEGGQKFRLPEELKVWTGMLVTVRLVLK 258 A +++W+ +I ++ VP G++ ++ + G + + +V TG VTVR V+ Sbjct: 561 AVTGADIVAWEDTQIQVKILRVPGGIYDIRVANAAGAASNIYDNFEVLTGDQVTVRFVIN 620 Query: 259 NVHTDFDTRVYVTGNVYELGAWQAERAAGPFYNQIVYRYPTWYCDISLPADSRIEFKIML 318 N T V++TGNV ELG W A GP YNQ+VY+YPTWY D+S+PA IEFK + Sbjct: 621 NATTALGQNVFLTGNVSELGNWDPNNAIGPMYNQVVYQYPTWYYDVSVPAGQTIEFKFLK 680 Query: 319 RSGSGMMRLEQGEAHCFHTPAEGVSEIIAEWQ 350 + GS + E G F TP G + + WQ Sbjct: 681 KQGS-TVTWEGGANRTFTTPTSGTATVNVNWQ 711 >sp|P43379|CDGU_BACCI Cyclomaltodextrin glucanotransferase precursor (Cyclodextrin-glycosyltransferase) (CGTase) pir||A58800 cyclomaltodextrin glucanotransferase (EC 2.4.1.19) precursor [validated] - Bacillus circulans (strain 251) emb|CAA55023.1| cyclomaltodextrin glucanotransferase [Bacillus circulans] Length = 713 Score = 245 bits (626), Expect = 1e-63, Method: Compositional matrix adjust. Identities = 130/335 (38%), Positives = 184/335 (54%), Gaps = 10/335 (2%) Query: 23 GLPVVYYGTEQYMSGGDDPHNRAMMSSFSRETTXXXXVAQLNRLRACNLAIGYGSTKLLY 82 G+P +YYGTEQYMSGG DP NRA + SFS TT + +L LR CN AI YGST+ + Sbjct: 381 GVPAIYYGTEQYMSGGTDPDNRARIPSFSTSTTAYQVIQKLAPLRKCNPAIAYGSTQERW 440 Query: 83 VNSGVLVFERSCKRDVALILINQEDNAQI-LPSLATSLPQGAYSSLLKDIYPDHPAVAGA 141 +N+ VL++ER +VA++ +N+ NA + L TSLPQG+Y+ +L + + G+ Sbjct: 441 INNDVLIYERKFGSNVAVVAVNRNLNAPASISGLVTSLPQGSYNDVLGGLLNGNTLSVGS 500 Query: 142 EGDMQNVMLAPRSAFVYSYESDLEKPAAVHVYPKAAAPGSEFIIRGRHLGDT------GC 195 G N LA V+ Y + P HV P A PG I GR G + G Sbjct: 501 GGAASNFTLAAGGTAVWQYTAATATPTIGHVGPMMAKPGVTITIDGRGFGSSKGTVYFGT 560 Query: 196 LTVGGCEAAVISWQSGKIVARVPAVPAGVHPVQGLSEGGQKFRLPEELKVWTGMLVTVRL 255 V G + + SW+ +I ++PAV G + ++ + G + + +V +G V+VR Sbjct: 561 TAVSGAD--ITSWEDTQIKVKIPAVAGGNYNIKVANAAGTASNVYDNFEVLSGDQVSVRF 618 Query: 256 VLKNVHTDFDTRVYVTGNVYELGAWQAERAAGPFYNQIVYRYPTWYCDISLPADSRIEFK 315 V+ N T VY+TG+V ELG W +A GP YNQ+VY+YP WY D+S+PA IEFK Sbjct: 619 VVNNATTALGQNVYLTGSVSELGNWDPAKAIGPMYNQVVYQYPNWYYDVSVPAGKTIEFK 678 Query: 316 IMLRSGSGMMRLEQGEAHCFHTPAEGVSEIIAEWQ 350 + + GS + E G H F P+ G + I WQ Sbjct: 679 FLKKQGS-TVTWEGGSNHTFTAPSSGTATINVNWQ 712 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.319 0.136 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 566,996,579 Number of extensions: 23162928 Number of successful extensions: 44607 Number of sequences better than 1.0e-05: 80 Number of HSP's gapped: 44469 Number of HSP's successfully gapped: 80 Length of query: 367 Length of database: 757,237,578 Length adjustment: 127 Effective length of query: 240 Effective length of database: 412,420,005 Effective search space: 98980801200 Effective search space used: 98980801200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 126 (53.1 bits)