BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0254.0032.p
         (339 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF 
           2,715,099 sequences; 757,237,578 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|NP_832212.1| Macrolide-efflux protein [Bacillus cereus ATCC ...   112   1e-23
ref|NP_244747.1| multidrug resistance protein [Bacillus halodura...   100   5e-20
ref|ZP_00098068.1| COG0477: Permeases of the major facilitator s...    55   2e-06

>ref|NP_832212.1| Macrolide-efflux protein [Bacillus cereus ATCC 14579]
 gb|AAP09413.1| Macrolide-efflux protein [Bacillus cereus ATCC 14579]
          Length = 417

 Score =  112 bits (281), Expect = 1e-23,   Method: Compositional matrix adjust.
 Identities = 78/266 (29%), Positives = 132/266 (49%), Gaps = 3/266 (1%)

Query: 38  PAQQSMTRSIVREDQLLQATSLNGIVNQGSKIAGPLLGGVALTLLSPQWCILLNAIFRVG 97
           PAQQ++TR IV  D L +A S+NGIV QG+KI GPL+GG+ L+   P++C+++ AI  + 
Sbjct: 121 PAQQTLTRQIVSPDLLTKAVSINGIVEQGTKIVGPLIGGMLLSWFQPEFCLIIRAISCLL 180

Query: 98  SYLLLLSVKNIHTGDEHPAAEVQEERVPLRKMWAEGWSFMLRSRLLLHTMLFGLTGALAI 157
           + L+L  +  I   +      V++++      W +GW ++L +R++L TM+F       +
Sbjct: 181 ATLVL--IPTIKFKETISKEFVEQQKQSTWTAWVQGWGYVLSNRIILSTMIFVTIAMAVL 238

Query: 158 QIIDFQFTSLFRVISPANESLLGWMXXXXXXXXXXXILLLGKLKPGTSYGLRLXXXXXXX 217
           Q++D QF +LF+ + P ++S +G++            LL  KLK    YG  +       
Sbjct: 239 QLVDSQFPTLFKSLFPHDKSKMGYIISIIGLGGILGALLTQKLKQ-FQYGWVVCGGMVLM 297

Query: 218 XXXXXXXXXXXXXASVIWVXXXXXXXXXXXXXXXVAFNYCLQKETPPHMTGRIFGIQSSI 277
                           +                 V+    LQ E+     GR+FGIQSS+
Sbjct: 298 GIGFGGIGLITPGTVFVLAYLISFIAGIGSGLMLVSNQVILQIESDQDQVGRVFGIQSSL 357

Query: 278 LGLVMITAPLLGGWLVQVAGPGRIFM 303
              V+I +P + G LV + G  ++++
Sbjct: 358 TNAVLIISPAMSGPLVHLFGVTQLYV 383


>ref|NP_244747.1| multidrug resistance protein [Bacillus halodurans]
 pir||H84134 multidrug resistance protein BH3880 [imported] - Bacillus
           halodurans (strain C-125)
 dbj|BAB07599.1| multidrug resistance protein [Bacillus halodurans]
          Length = 424

 Score =  100 bits (249), Expect = 5e-20,   Method: Compositional matrix adjust.
 Identities = 72/263 (27%), Positives = 117/263 (44%), Gaps = 4/263 (1%)

Query: 36  NVPAQQSMTRSIVREDQLLQATSLNGIVNQGSKIAGPLLGGVALTLLSPQWCILLNAI-F 94
           + PAQQS+ R ++R+D  LQA + NG V+Q +KI GP+ GG  LT  SP   ILLN I F
Sbjct: 120 HYPAQQSLLRQLIRDDLRLQAITWNGAVSQAAKIIGPMGGGALLTAFSPHTLILLNGIAF 179

Query: 95  RVGSYLLLLSVKNIHTGDEHPAAEVQEERVPLRKMWAEGWSFMLRSRLLLHTMLFGLTGA 154
            + + LL    +N+   ++  + E Q         W  GW  + + +++  +    L G 
Sbjct: 180 LLSALLLTTLFRNVKVVEKDTSLENQSS---FWASWKAGWQVVFKRKVIYFSFAIALLGM 236

Query: 155 LAIQIIDFQFTSLFRVISPANESLLGWMXXXXXXXXXXXILLLGKLKPGTSYGLRLXXXX 214
             IQ++D QF  LFR I+ A   ++ W+           +  LG++K   + G+ L    
Sbjct: 237 SVIQMVDIQFPVLFRQIAAAQPQMVAWIMAAIGTGAIAMMAFLGRMKQLRNVGMWLAGAM 296

Query: 215 XXXXXXXXXXXXXXXXASVIWVXXXXXXXXXXXXXXXVAFNYCLQKETPPHMTGRIFGIQ 274
                             ++                 V  NY +Q E       ++ GI 
Sbjct: 297 ICIGIAFLGIGMLQEGFLMMIPIGLGLIAGLGTGTSMVVSNYIIQTEPREQEVSQVAGIY 356

Query: 275 SSILGLVMITAPLLGGWLVQVAG 297
            S++ + ++  PLLG WL+ + G
Sbjct: 357 QSLISMAILLPPLLGAWLIHLIG 379


>ref|ZP_00098068.1| COG0477: Permeases of the major facilitator superfamily
           [Desulfitobacterium hafniense]
          Length = 421

 Score = 55.5 bits (132), Expect = 2e-06,   Method: Compositional matrix adjust.
 Identities = 36/128 (28%), Positives = 63/128 (49%), Gaps = 14/128 (10%)

Query: 36  NVPAQQSMTRSIVREDQLLQATSLNGIVNQGSKIAGPLLGGVALTLLSPQWCILLNAIFR 95
           +VP +QS    +V  + L  A +LN  +  G+++ GP + GV + +  P WC L+N +  
Sbjct: 130 DVPTRQSFIIELVGREHLANAIALNSAIFNGARLVGPAIAGVIMGIWGPMWCFLINGLSF 189

Query: 96  VGSYLLLLSVKNIHTGDEHPAAEVQEERVP----LRKMWAEGWSFMLRS-RLLLHTMLFG 150
           +G   +L+ V         PA   QE+  P    LRK    G S++ ++  +L+  M+ G
Sbjct: 190 IGVLAILIFV---------PAIPHQEKITPKKETLRKDILNGLSYIRKTPSILIVMMMMG 240

Query: 151 LTGALAIQ 158
               +A+ 
Sbjct: 241 FLSTIAMN 248


  Database: All non-redundant GenBank CDS
  translations+PDB+SwissProt+PIR+PRF
    Posted date:  Mar 24, 2004  1:53 AM
  Number of letters in database: 757,237,578
  Number of sequences in database:  2,715,099
  
Lambda     K      H
   0.324    0.139    0.434 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 2715099
Number of Hits to DB: 337,626,547
Number of extensions: 10802285
Number of successful extensions: 28178
Number of sequences better than 1.0e-05: 4
Number of HSP's gapped: 28193
Number of HSP's successfully gapped: 4
Length of query: 339
Length of database: 757,237,578
Length adjustment: 126
Effective length of query: 213
Effective length of database: 415,135,104
Effective search space: 88423777152
Effective search space used: 88423777152
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 126 (53.1 bits)