BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0254.0031.p (78 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_832212.1| Macrolide-efflux protein [Bacillus cereus ATCC ... 64 1e-09 ref|NP_244747.1| multidrug resistance protein [Bacillus halodura... 63 2e-09 >ref|NP_832212.1| Macrolide-efflux protein [Bacillus cereus ATCC 14579] gb|AAP09413.1| Macrolide-efflux protein [Bacillus cereus ATCC 14579] Length = 417 Score = 63.9 bits (154), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 28/60 (46%), Positives = 39/60 (65%) Query: 9 NSLLHNKVYMRVYSAYATAAFGDWFDSLAIQVLVGYRWHASPLMLALIPVAVALPSVLLG 68 LL N+VY++V+SAY+ G + D LAI +VG+ W P M+ LIPVA ALP ++ G Sbjct: 7 KGLLGNRVYLQVFSAYSLLMLGVFIDMLAIMTIVGFEWEVDPTMIGLIPVAYALPGIIFG 66 >ref|NP_244747.1| multidrug resistance protein [Bacillus halodurans] pir||H84134 multidrug resistance protein BH3880 [imported] - Bacillus halodurans (strain C-125) dbj|BAB07599.1| multidrug resistance protein [Bacillus halodurans] Length = 424 Score = 63.2 bits (152), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 27/65 (41%), Positives = 43/65 (66%) Query: 4 SVKQGNSLLHNKVYMRVYSAYATAAFGDWFDSLAIQVLVGYRWHASPLMLALIPVAVALP 63 + K+ +L Y +++++Y+ + G WFD +A+ VL GY W+A PL+LA IP+A ALP Sbjct: 3 TTKKRETLWQQPGYKKLFTSYSISMVGQWFDLVALLVLFGYVWNADPLLLAFIPIAFALP 62 Query: 64 SVLLG 68 +L G Sbjct: 63 QMLFG 67 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.323 0.135 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 99,935,761 Number of extensions: 3149504 Number of successful extensions: 5451 Number of sequences better than 1.0e-05: 2 Number of HSP's gapped: 5451 Number of HSP's successfully gapped: 2 Length of query: 78 Length of database: 757,237,578 Length adjustment: 48 Effective length of query: 30 Effective length of database: 626,912,826 Effective search space: 18807384780 Effective search space used: 18807384780 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 120 (50.8 bits)