BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0254.0020.p (416 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_832903.1| Bicyclomycin resistance protein [Bacillus cereu... 337 2e-91 ref|NP_388412.1| similar to antibiotic resistance protein [Bacil... 337 5e-91 ref|NP_845592.1| transporter, putative [Bacillus anthracis str. ... 333 4e-90 ref|ZP_00026318.1| COG0477: Permeases of the major facilitator s... 316 6e-85 ref|NP_355096.1| AGR_C_3872p [Agrobacterium tumefaciens] >gi|253... 307 3e-82 >ref|NP_832903.1| Bicyclomycin resistance protein [Bacillus cereus ATCC 14579] gb|AAP10104.1| Bicyclomycin resistance protein [Bacillus cereus ATCC 14579] Length = 396 Score = 337 bits (865), Expect = 2e-91, Method: Compositional matrix adjust. Identities = 188/396 (47%), Positives = 236/396 (59%), Gaps = 1/396 (0%) Query: 19 VKHAQASAASKAVPGWITFLLAASCGLIVANLYYAQTLVGPISAATHLSSGAAGLIVTLT 78 + + + A K + + LLA +CG+IVANLYYAQ L+G IS LS+ +AGLIVTLT Sbjct: 1 MSNFKNKAIDKDISSGLIILLATACGIIVANLYYAQPLIGLISNEIGLSNSSAGLIVTLT 60 Query: 79 QIGYVIGLLFVVPLSDIIENRRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIGLGSVA 138 QIGYV+GLLF+VPL DI+EN++ IGLGSVA Sbjct: 61 QIGYVVGLLFLVPLGDIVENKKLILILLFLSAFALISMVFVKSATLLLIASFFIGLGSVA 120 Query: 139 AQILVPYASYLAAEEHRGRVVGNVMSGLLLGIMFARPLASFVSGLWGWHAIFAISAVIIA 198 AQ+LVP SYL++E RGRVVGNVMSGLLLGIM ARP++S V+ +WGW+AIFA+SA +I Sbjct: 121 AQVLVPLVSYLSSENARGRVVGNVMSGLLLGIMLARPISSLVADMWGWNAIFALSATVII 180 Query: 199 LLTILLSRVLPKRQPIPTMNYGGLIRSLGTLLKTTPVLRRRALYQASLFGTFSLFWTTVS 258 +L +LS+VLP R+P NY L+ S+ LL+TTP+LRRRA+Y A +FG FSLFWTTV Sbjct: 181 VLAFVLSKVLPTRKPKAKTNYIALLNSMWQLLRTTPILRRRAIYHACVFGAFSLFWTTVP 240 Query: 259 LRL-ADTYHLSQQXXXXXXXXXXXXXXXXXXXXXXXDRGWTRPLTGXXXXXXXXXXXXXX 317 L L + +H SQ D GWTRP TG Sbjct: 241 LLLFSPAFHFSQTAIALYALVGITGAIAAPIGGRLADLGWTRPATGIALTVVIISLILPL 300 Query: 318 XXQSDSKVTLGLLVVGAIILDMGVSGNLVLGQRAIYSLGNEARGRLNGLFMAIFFIGGAI 377 QS S + +LV+ AI+LDMGVS NLVL QR I+SL E R RLNGLFMAIFF+GGA+ Sbjct: 301 FIQSSSSFGIAVLVIAAILLDMGVSANLVLSQRLIFSLSPEIRSRLNGLFMAIFFLGGAV 360 Query: 378 XXXXXXXXXXXXXXXXTTLIGLALPVLAFLYYLTEK 413 T IG+A P +A LY+ E+ Sbjct: 361 GSFIGGWAYALGGWNLTLWIGIAFPTIALLYFAREE 396 >ref|NP_388412.1| similar to antibiotic resistance protein [Bacillus subtilis] pir||D69779 antibiotic resistance protein homolog ydeR - Bacillus subtilis dbj|BAA19365.1| PROBABLE INTEGRAL MEMBRANE PROTEIN, SIMILAR TO CHLORAMPHENICOL RESISTANCE PROTEIN OF STREPTOMYCES VENEZUELAE. [Bacillus subtilis] emb|CAB12338.1| ydeR [Bacillus subtilis subsp. subtilis str. 168] Length = 395 Score = 337 bits (863), Expect = 5e-91, Method: Compositional matrix adjust. Identities = 194/380 (51%), Positives = 235/380 (61%), Gaps = 1/380 (0%) Query: 35 ITFLLAASCGLIVANLYYAQTLVGPISAATHLSSGAAGLIVTLTQIGYVIGLLFVVPLSD 94 +T LLA +CG+IVANLYYAQ LVG IS A LS AGLIVTLTQIGYV GLLF+VPL D Sbjct: 16 LTLLLATACGIIVANLYYAQPLVGLISKAIGLSPSGAGLIVTLTQIGYVAGLLFLVPLGD 75 Query: 95 IIENRRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIGLGSVAAQILVPYASYLAAEEH 154 IIEN++ +GLGS+AAQ+LVP+ASYLA++ Sbjct: 76 IIENKKLIVVSLLLSAAALTLTAFVKHGTLFLAAAFFVGLGSIAAQVLVPFASYLASDAA 135 Query: 155 RGRVVGNVMSGLLLGIMFARPLASFVSGLWGWHAIFAISAVIIALLTILLSRVLPKRQPI 214 RGRVVGNVMSGLLLGIM +RP++S V+ +WGW+AIFA+SAV+ +L I+LS+VLP R+P Sbjct: 136 RGRVVGNVMSGLLLGIMLSRPISSLVADIWGWNAIFALSAVVSVILAIVLSKVLPARKPT 195 Query: 215 PTMNYGGLIRSLGTLLKTTPVLRRRALYQASLFGTFSLFWTTVSLRLA-DTYHLSQQXXX 273 NY L+ S+ LL+TTPVLRRRA+Y A +FG FSLFWTTV L L+ +H SQ+ Sbjct: 196 ANTNYTALLGSMWKLLRTTPVLRRRAIYHAFVFGAFSLFWTTVPLLLSGPDFHFSQKAIA 255 Query: 274 XXXXXXXXXXXXXXXXXXXXDRGWTRPLTGXXXXXXXXXXXXXXXXQSDSKVTLGLLVVG 333 DRG TR TG QS S V + +LV Sbjct: 256 LYALVGIAGAVTAPIGGRLADRGLTRLATGIALGVVVVSLLLPLMIQSSSPVGITVLVAA 315 Query: 334 AIILDMGVSGNLVLGQRAIYSLGNEARGRLNGLFMAIFFIGGAIXXXXXXXXXXXXXXXX 393 AI+LDMGVS NLVL QRAI+SL E R RLNGLFMAIFF+GGAI Sbjct: 316 AILLDMGVSANLVLSQRAIFSLAPEIRSRLNGLFMAIFFLGGAIGSSIGGWAYASGGWST 375 Query: 394 TTLIGLALPVLAFLYYLTEK 413 IG+A PV+A LY++TEK Sbjct: 376 ALWIGIAFPVIALLYFITEK 395 >ref|NP_845592.1| transporter, putative [Bacillus anthracis str. Ames] gb|AAP27078.1| transporter, putative [Bacillus anthracis str. Ames] Length = 389 Score = 333 bits (854), Expect = 4e-90, Method: Compositional matrix adjust. Identities = 187/387 (48%), Positives = 232/387 (59%), Gaps = 8/387 (2%) Query: 28 SKAVPGWITFLLAASCGLIVANLYYAQTLVGPISAATHLSSGAAGLIVTLTQIGYVIGLL 87 K + + LLA +CG+IVANLYYAQ L+G IS LS+ +AGLIVTLTQIGYV+GLL Sbjct: 10 DKDISSGLIILLATACGIIVANLYYAQPLIGVISNEIGLSNSSAGLIVTLTQIGYVVGLL 69 Query: 88 FVVPLSDIIENRRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIGLGSVAAQILVPYAS 147 F+VPL DI+EN++ IGLGSVAAQ+LVP S Sbjct: 70 FLVPLGDIVENKKLILILLFLSAFALISMVFVKSATLLLIASFFIGLGSVAAQVLVPLVS 129 Query: 148 YLAAEEHRGRVVGNVMSGLLLGIMFARPLASFVSGLWGWHAIFAISAVIIALLTILLSRV 207 YL++E RGRVVGNVMSGLLLGIM ARP++S V+ +WGW+AIFA+SA +I +L +LS+V Sbjct: 130 YLSSENARGRVVGNVMSGLLLGIMLARPISSLVADMWGWNAIFALSATVIIVLAFVLSKV 189 Query: 208 LPKRQPIPTMNYGGLIRSLGTLLKTTPVLRRRALYQASLFGTFSLFWTTVSLRLAD-TYH 266 LP R+P NY L+ S+ LL+TTP+LRRRA+Y A +FG FSLFWTTV L L+ +H Sbjct: 190 LPTRKPQVKTNYIALLNSMWQLLRTTPILRRRAIYHACVFGAFSLFWTTVPLLLSSPAFH 249 Query: 267 LSQQXXXXXXXXXXXXXXXXXXXXXXXDRGWTRPLTGXXXXXXXXXXXXXXXXQSDSKVT 326 SQ D GWTR TG QS S + Sbjct: 250 FSQTAIALYALVGITGAIAAPIGGRLADLGWTRSATGIALTVVIISLLLPLIIQSSSPIG 309 Query: 327 LGLLVVGAIILDMGVSGNLVLGQRAIYSLGNEARGRLNGLFMAIFFIGGAIXXXXXXXXX 386 + +LV+ AI+LDMGVS NLVL QR I+SL E R RLNGLFMAIFF+GGA+ Sbjct: 310 IAVLVIAAILLDMGVSANLVLSQRLIFSLSPEIRSRLNGLFMAIFFLGGAV-------GS 362 Query: 387 XXXXXXXTTLIGLALPVLAFLYYLTEK 413 T IG+A P +A LY+ EK Sbjct: 363 FIGGWNLTLWIGIAFPTIALLYFAREK 389 >ref|ZP_00026318.1| COG0477: Permeases of the major facilitator superfamily [Ralstonia metallidurans] Length = 412 Score = 316 bits (810), Expect = 6e-85, Method: Compositional matrix adjust. Identities = 191/414 (46%), Positives = 237/414 (57%), Gaps = 7/414 (1%) Query: 3 GGIHMNQVNQASRGMNVKHAQASAASKAVPGWITFLLAASCGLIVANLYYAQTLVGPISA 62 G I++ + A + A A AA WIT LLA +CG+IVANLYYAQ LVGPI+ Sbjct: 4 GPIYVPVESAAMTSATAQRADAPAA------WITPLLATACGMIVANLYYAQPLVGPIAR 57 Query: 63 ATHLSSGAAGLIVTLTQIGYVIGLLFVVPLSDIIENRRXXXXXXXXXXXXXXXXXXXXXX 122 A LS AGLIVTL QIGY +GLL +VPL DI+ENR+ Sbjct: 58 ALELSPEIAGLIVTLIQIGYCVGLLLLVPLGDIVENRKLVLALIAGCAVALVAAALATHA 117 Query: 123 XXXXXXXXXIGLGSVAAQILVPYASYLAAEEHRGRVVGNVMSGLLLGIMFARPLASFVSG 182 IGL SVA Q+LVP+A++LA E RGR VGNV SGLL+GIM ARP++S V+ Sbjct: 118 SVFLIAGCAIGLCSVAVQVLVPFAAHLAPEHARGRAVGNVTSGLLMGIMLARPVSSLVAD 177 Query: 183 LWGWHAIFAISAVIIALLTILLSRVLPKRQPIPTMNYGGLIRSLGTLLKTTPVLRRRALY 242 L+GWH IFA SAV + LL ++LSR LP+RQP P +NY ++ S+G LL+T PVLRRRA+Y Sbjct: 178 LFGWHTIFAASAVAMVLLGVVLSRKLPQRQPAPGVNYVEMLGSMGHLLRTQPVLRRRAMY 237 Query: 243 QASLFGTFSLFWTTVSLRLA-DTYHLSQQXXXXXXXXXXXXXXXXXXXXXXXDRGWTRPL 301 QAS+FG FSLFWTT L LA +H+SQ DRG +R + Sbjct: 238 QASMFGVFSLFWTTTPLYLAGPAFHMSQTGIAVFALVGVAGAIAAPMAGRYADRGHSRQM 297 Query: 302 TGXXXXXXXXXXXXXXXXQSDSKVTLGLLVVGAIILDMGVSGNLVLGQRAIYSLGNEARG 361 T S ++L L V A++LD VS NLV+GQRAI+SL E R Sbjct: 298 TAIALALGAGSFLLSRIGVEGSLMSLAALTVAAVVLDFAVSANLVIGQRAIFSLSAEHRS 357 Query: 362 RLNGLFMAIFFIGGAIXXXXXXXXXXXXXXXXTTLIGLALPVLAFLYYLTEKKQ 415 RLNGL+MAIFF+GGAI + IG ALP +A LY+ TE Sbjct: 358 RLNGLYMAIFFVGGAIGSSVGAWAYARAGWPLASWIGFALPAIALLYFRTEPAS 411 >ref|NP_355096.1| AGR_C_3872p [Agrobacterium tumefaciens] pir||H97615 antibiotic resistance protein homolog ydeR [imported] - Agrobacterium tumefaciens (strain C58, Cereon) gb|AAK87881.1| AGR_C_3872p [Agrobacterium tumefaciens str. C58 (Cereon)] Length = 405 Score = 307 bits (787), Expect = 3e-82, Method: Compositional matrix adjust. Identities = 178/402 (44%), Positives = 229/402 (56%), Gaps = 5/402 (1%) Query: 14 SRGMNVKHAQASAASKAVPGWITFLLAASCGLIVANLYYAQTLVGPISAATHLSSGAAGL 73 +R ++ +A++ S A +TFLLA +CGLI ANLYY Q L G I LS+GA GL Sbjct: 7 TRVIDGDDVEATSISPA----LTFLLATACGLIAANLYYGQPLAGIIGTELGLSAGATGL 62 Query: 74 IVTLTQIGYVIGLLFVVPLSDIIENRRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIG 133 IVTLTQIGY IGLLFVVPL D++ENR+ +G Sbjct: 63 IVTLTQIGYGIGLLFVVPLGDLVENRKLVVSSVSMAVLSLVAAAFAPHAAPFLIAAFLVG 122 Query: 134 LGSVAAQILVPYASYLAAEEHRGRVVGNVMSGLLLGIMFARPLASFVSGLWGWHAIFAIS 193 + SVA Q++VPYA+++A RGRVVGNVMSGL+ GIM ARP++S +S + W +F S Sbjct: 123 VSSVAVQVIVPYAAHMAPHAIRGRVVGNVMSGLMAGIMLARPVSSLLSEVVSWRGVFLTS 182 Query: 194 AVIIALLTILLSRVLPKRQPIPTMNYGGLIRSLGTLLKTTPVLRRRALYQASLFGTFSLF 253 A ++ALL ++L R+LP R P ++YG L+ S+G L TP+LRRRA+Y A LF FSLF Sbjct: 183 AAVMALLAVVLFRLLPVRMPEARLSYGALMASMGRLALHTPILRRRAIYHAFLFAAFSLF 242 Query: 254 WTTVSLRLADT-YHLSQQXXXXXXXXXXXXXXXXXXXXXXXDRGWTRPLTGXXXXXXXXX 312 WTT L L+ ++LSQ DRGWTR T Sbjct: 243 WTTTPLYLSGPHFNLSQGEIALFALAGAAGTVAAPIAGRMADRGWTRVATLFALVSVALS 302 Query: 313 XXXXXXXQSDSKVTLGLLVVGAIILDMGVSGNLVLGQRAIYSLGNEARGRLNGLFMAIFF 372 S + L +LVV AI+LD GV+ NLVLGQRAI++LG E R RLNG++MA FF Sbjct: 303 FAVTHLAPEGSHLALAILVVAAIVLDFGVTTNLVLGQRAIFTLGAEFRSRLNGIYMATFF 362 Query: 373 IGGAIXXXXXXXXXXXXXXXXTTLIGLALPVLAFLYYLTEKK 414 +GGAI + IG ALPV A LY+LTEK+ Sbjct: 363 MGGAIGSAVGGWAYAVGEWPAASWIGFALPVAALLYFLTEKR 404 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.325 0.139 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 431,639,202 Number of extensions: 13809787 Number of successful extensions: 56412 Number of sequences better than 1.0e-05: 96 Number of HSP's gapped: 56262 Number of HSP's successfully gapped: 105 Length of query: 416 Length of database: 757,237,578 Length adjustment: 128 Effective length of query: 288 Effective length of database: 409,704,906 Effective search space: 117995012928 Effective search space used: 117995012928 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 127 (53.5 bits)