BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0254.0017.p (697 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value pir||A54078 methyl-accepting chemotaxis protein mcpB - Bacillus ... 174 5e-42 ref|NP_391004.1| methyl-accepting chemotaxis protein [Bacillus s... 171 5e-41 ref|NP_347441.1| Methyl-accepting chemotaxis protein [Clostridiu... 169 2e-40 ref|NP_981444.1| methyl-accepting chemotaxis protein [Bacillus c... 167 1e-39 ref|NP_847435.1| methyl-accepting chemotaxis protein [Bacillus a... 164 5e-39 >pir||A54078 methyl-accepting chemotaxis protein mcpB - Bacillus subtilis Length = 662 Score = 174 bits (442), Expect = 5e-42, Method: Compositional matrix adjust. Identities = 152/546 (27%), Positives = 269/546 (49%), Gaps = 35/546 (6%) Query: 166 REYFTEAFK--GKSFISDAIVSKSNNKLLLVFSQPVKSADGKVLGVLAMTVASDFFLDKL 223 R ++ +A K G+ ++D V+ S+ +++ +Q +K DG GV+AM + D L+++ Sbjct: 138 RPWYQDAMKAGGEIVVTDPYVAASDGSMVITIAQELK--DGS--GVVAMDITIDKLLEQM 193 Query: 224 GNININAKGKIEVLSRGGIVLYNSSDPARIGQDVGDDPGIKPLLETRATDELEIQTTELG 283 I + +G + ++ N + A G+ + + A D E+Q T L Sbjct: 194 KQIKVGKEGYAFIATK------NKTYVAHKNHKAGEKLSGDWVAKMYANDSGELQYT-LN 246 Query: 284 TEYLRI----NKIPKADLTISV-IDSYDDIERPIQDMLMKILIVTVIALLVAIGFGLLLS 338 E ++ N++ + ++ +D D + + M +LI +++A + I L + Sbjct: 247 NEDKKMTYTTNELTGWKIAGTMYMDEIKDASKSVLTTGMIVLIASIVAGGILI---LFIV 303 Query: 339 RSITKPVVTLTSRFKTLATGDLTVTADGSYKSEFKDLADSFNTMVQQNKALITNMNDSIT 398 RSITKP+ L KT++ GDLT T + K E +L +SFN M Q ++LI+ + DS+ Sbjct: 304 RSITKPLKRLVQSSKTISRGDLTETIEIHSKDELGELGESFNEMGQSLRSLISAIQDSVN 363 Query: 399 ILKTSTHELEETSKQAARSVNETSVTSMGIAQAMELQSNDTEQIVNKFYGFSEKFAGMND 458 + S+ +L ++ Q +++ I A+E SN E+ K S + MN+ Sbjct: 364 NVAASSEQLTASAGQTSKATEH-------ITMAIEQFSNGNEEQSRKVESSSHQLNLMNE 416 Query: 459 NAQAVRQRAEEIT-------QVFHTSNQVVEGLIEISSKNEQEVQKISAITVKLQDSSNS 511 Q V Q + +IT ++ T + V+ + + Q VQ+ A+ L+ S Sbjct: 417 GLQQVSQTSSDITKASIQSTEIAGTGEKFVQQTVGQMNSINQSVQQAEAVVKGLEGKSKD 476 Query: 512 ISNITGAINQIAAQTNLLALNASIEAARAGEHGRGFAVVASEIRKLAEQSSRQSNEIYEI 571 I++I IN IA QTNLLALNA+IEAARAGE GRGF+VVA E+RKLA QS+ + EI ++ Sbjct: 477 ITSILRVINGIADQTNLLALNAAIEAARAGESGRGFSVVAEEVRKLAVQSADSAKEIEKL 536 Query: 572 IQQNLAFVAENNDSVMEINQISAQQDELVGQTQTAFQTILDKITEITVQIQSMADEIAHM 631 IQ+ +A + + E+NQ + T+ +FQ+I EI ++Q+M + + Sbjct: 537 IQEIVAEIDTSLHMFKEVNQEVQSGLVVTDNTKESFQSIFSMTNEIAGKLQTMNSTVEQL 596 Query: 632 QKDKDEVLDSAQSLXXXXXXXXXXXXXXXXXMMEQSSAVDQLAEMVGTIDQLSKSLAEAA 691 V + + EQ +++++++ T+ Q+++ L + Sbjct: 597 SDRSQHVSAAVSGIADVSKESSASIQDIAASAEEQLASMEEISSSATTLAQMAEELRDLT 656 Query: 692 SKFRIE 697 +F+IE Sbjct: 657 KQFKIE 662 >ref|NP_391004.1| methyl-accepting chemotaxis protein [Bacillus subtilis] sp|P39215|MCPB_BACSU Methyl-accepting chemotaxis protein mcpB (H3) gb|AAA20554.1| transmembrane receptor [Bacillus subtilis] emb|CAB15104.1| methyl-accepting chemotaxis protein [Bacillus subtilis subsp. subtilis str. 168] Length = 662 Score = 171 bits (434), Expect = 5e-41, Method: Compositional matrix adjust. Identities = 153/547 (27%), Positives = 272/547 (49%), Gaps = 37/547 (6%) Query: 166 REYFTEAFK--GKSFISDAIVSKSNNKLLLVFSQPVKSADGKVLGVLAMTVASDFFLDKL 223 R ++ +A K G+ ++D V+ S+ +++ +Q +K DG GV+AM + D L+++ Sbjct: 138 RPWYQDAMKAGGEIVVTDPYVAASDGSMVITIAQELK--DGS--GVVAMDITIDKLLEQM 193 Query: 224 GNININAKGKIEVLSRGGIVLYNSSDPARIGQDVGDDPGIKPLLETRATDELEIQTTELG 283 I + +G + ++ N + A G+ + + A D E+Q T L Sbjct: 194 KQIKVGKEGYAFIATK------NKTYVAHKNHKAGEKLSGDWVAKMYANDSGELQYT-LN 246 Query: 284 TEYLRI----NKIPKADLTISV-IDSYDDIERPIQDMLMKILIVTVIALLVAIGFGLLLS 338 E ++ N++ + ++ +D D + + M +LI +++A + I L + Sbjct: 247 NEDKKMTYTTNELTGWKIAGTMYMDEIKDASKSVLTTGMIVLIASIVAGGILI---LFIV 303 Query: 339 RSITKPVVTLTSRFKTLATGDLTVTADGSYKSEFKDLADSFNTMVQQNKALITNMNDSIT 398 RSITKP+ L KT++ GDLT T + K E +L +SFN M Q ++LI+ + +S+ Sbjct: 304 RSITKPLKRLVQSSKTISRGDLTETIEIHSKDELGELGESFNEMGQSLRSLISAIQNSVN 363 Query: 399 ILKTSTHELEETSKQAARSVNETSVTSMGIAQAMELQSNDTEQIVNKFYGFSEKFAGMND 458 + S+ +L ++ Q +++ I A+E SN E+ K S + MN+ Sbjct: 364 NVAASSEQLTASAGQTSKATEH-------ITMAIEQFSNGNEEQSRKVESSSHQLNLMNE 416 Query: 459 NAQAVRQRAEEITQVFHTSNQVV---EGLIE-----ISSKNEQEVQKISAITVKLQDSSN 510 Q V Q + +IT+ S ++ E ++ ++S N Q VQ+ A+ L+ S Sbjct: 417 GLQQVSQTSSDITKASIQSTEIAGTGEKFVQQTGGQMNSIN-QSVQQAEAVVKGLEGKSK 475 Query: 511 SISNITGAINQIAAQTNLLALNASIEAARAGEHGRGFAVVASEIRKLAEQSSRQSNEIYE 570 I++I IN IA QTNLLALNA+IEAARAGE GRGF+VVA E+RKLA QS+ + EI + Sbjct: 476 DITSILRVINGIADQTNLLALNAAIEAARAGESGRGFSVVAEEVRKLAVQSADSAKEIEK 535 Query: 571 IIQQNLAFVAENNDSVMEINQISAQQDELVGQTQTAFQTILDKITEITVQIQSMADEIAH 630 +IQ+ +A + + E+NQ + T+ +FQ+I EI ++Q+M + Sbjct: 536 LIQEIVAEIDTSLHMFKEVNQEVQSGLVVTDNTKESFQSIFSMTNEIAGKLQTMNSTVEQ 595 Query: 631 MQKDKDEVLDSAQSLXXXXXXXXXXXXXXXXXMMEQSSAVDQLAEMVGTIDQLSKSLAEA 690 + V + + EQ +++++++ T+ Q+++ L + Sbjct: 596 LSDRSQHVSAAVSGIADVSKESSASIQDIAASAEEQLASMEEISSSATTLAQMAEELRDL 655 Query: 691 ASKFRIE 697 +F+IE Sbjct: 656 TKQFKIE 662 >ref|NP_347441.1| Methyl-accepting chemotaxis protein [Clostridium acetobutylicum] pir||B96999 methyl-accepting chemotaxis protein [imported] - Clostridium acetobutylicum gb|AAK78781.1| Methyl-accepting chemotaxis protein [Clostridium acetobutylicum] Length = 668 Score = 169 bits (429), Expect = 2e-40, Method: Compositional matrix adjust. Identities = 145/549 (26%), Positives = 267/549 (48%), Gaps = 36/549 (6%) Query: 166 REYFTEAFK--GKSFISDAIVSKSNNKLLLVFSQPVKSADGKVLGVLAMTVASDFFLDKL 223 R ++ A G+ + + S K+++ S+ +++ +GKV+GV++M ++ D KL Sbjct: 138 RPWYKNALNKTGEIVYTGPYKAASTGKMVISLSKTIEN-NGKVVGVVSMDLSLDELSKKL 196 Query: 224 GNININAKGKIEVLSRGGIVLYNSSDPARIG-----------QDVGDDPGIKPLLETRAT 272 N I G I V G+++ + D + +G +DV + Sbjct: 197 SNTKIGQSGYIFVTDSNGVMI-SHPDKSLLGNNSTVTALSYWKDVKKTSSGFETYSYKGK 255 Query: 273 DELEIQTTELGTEYLRINKIPKADLTISVIDSYDDIERPIQDMLMKILI-VTVIALLVAI 331 + + T+ T + + +P ++LT + IQ + I + + VI ++AI Sbjct: 256 QKYIVYNTDNRTGWKMMASMPTSELTSKT--------KSIQSAGITIFVLIAVIGTVIAI 307 Query: 332 GFGLLLSRSITKPVVTLTSRFKTLATGDLTVTADGSYKSEFKDLADSFNTMVQQNKALIT 391 +SRSIT ++ L FK + GDLT + K EF++L +SFN M + LI Sbjct: 308 ----FVSRSITSKIIILKEIFKKASQGDLTAEVNIRTKDEFEELGNSFNVMTTKIGELIL 363 Query: 392 NMNDSITILKTSTHELEETSKQAARSVNETSVTSMGIAQAMELQSNDTEQIVNKFYGFSE 451 ++ S ++ ++ ++ + + +VNE + + +AQ ++D + V +E Sbjct: 364 SLTSSSQVISKTSGDVNRMALETNNAVNEVATSIDQVAQGASETAHDIQSSVEAINNLAE 423 Query: 452 KFAGMNDNAQAVRQRAEEITQVFHTSNQVVEGLIEISSKNEQEVQKISAITVKLQDSSNS 511 K ++ + +++ ++ + +V+ L + + KN + + ++++ +++ ++ Sbjct: 424 KIDEIDKLTNDMISTSDKSNKLSNEGLEVMHSLTDKTEKNIEASKGVTSVVSAMKEETDK 483 Query: 512 ISNITGAINQIAAQTNLLALNASIEAARAGEHGRGFAVVASEIRKLAEQSSRQSNEIYEI 571 IS IT IN+IA QTNLLALNA+IEAARAGE G+GF+VVA EIRKLAEQS+ +NEI E+ Sbjct: 484 ISMITDTINEIADQTNLLALNAAIEAARAGEAGKGFSVVADEIRKLAEQSTSATNEIQEL 543 Query: 572 IQQNLAFVAENNDSV---MEI-NQISAQQDELVGQTQTAFQTILDKITEITVQIQSMADE 627 I + + + DS ME+ N I +Q E+V T+ F IL I EI QI+ + Sbjct: 544 I----SGIKDKTDSAVRSMELSNIIVGEQSEVVNDTKEIFNKILKCINEIIDQIKLVQSA 599 Query: 628 IAHMQKDKDEVLDSAQSLXXXXXXXXXXXXXXXXXMMEQSSAVDQLAEMVGTIDQLSKSL 687 ++K +++ ++ E ++ + + G ++ LS L Sbjct: 600 TIETNREKTQLIGRMHNISAVSEQSSASAEEVSATAEEVTAVMSEFTTSAGELNNLSSEL 659 Query: 688 AEAASKFRI 696 + KF+I Sbjct: 660 EKQIKKFKI 668 >ref|NP_981444.1| methyl-accepting chemotaxis protein [Bacillus cereus ATCC 10987] gb|AAS44052.1| methyl-accepting chemotaxis protein [Bacillus cereus ATCC 10987] Length = 666 Score = 167 bits (422), Expect = 1e-39, Method: Compositional matrix adjust. Identities = 140/526 (26%), Positives = 249/526 (47%), Gaps = 35/526 (6%) Query: 187 SNNKLLLVFSQPVKSADGKVLGVLAMTVASDFFLDKLGNININAKGKIEVLSRGGI-VLY 245 + KL + S+ + DG VLGV+AM V+ L NI N G++ +++ I ++Y Sbjct: 160 ATGKLSIGVSKAIVGQDGSVLGVVAMDVSLGTIQKLLHNIQYNNGGEMFIVNDKNIAIVY 219 Query: 246 NSSDPARIGQDVGDDPGIKPLLETRATDELEIQTTELG----TEYLRINKIPKADLTISV 301 P ++G+DV + +K L DE + T LG Y ++ K I + Sbjct: 220 ----PGQVGKDVSKESLVKSL----NKDETKFAVTTLGGNDVVAYSQL--FDKMKWKIGI 269 Query: 302 IDSYDDIERPIQDMLMKILIVTVIALLVAIGFGLLLSRSITKPVVTLTSRFKTLATGDLT 361 + D I+ + ++I+ I+L V I L SR + +P+ LT+ + +A GDLT Sbjct: 270 VYPKDTIDGLLNSTRNTVIIMACISLFVGIAASYLFSRRLARPLQLLTNHVQKVAEGDLT 329 Query: 362 VTADGSYKSEFKDLADSFNTMVQQNKALITNMNDSITILKTSTHELEETSKQAARSVNET 421 + + K E +L + FN MV+Q +++ + +S++ ++ ST+ L + NET Sbjct: 330 LQMKVTSKDEVGELTNHFNHMVEQMNEMVSKIKNSVSTVQQSTNNLHYLT-------NET 382 Query: 422 SVTSMGIAQAMELQSNDTEQIVNKFYGFSEKFAGMNDNAQAVRQRAEEITQVFHTSNQVV 481 S ++ AM+ S + N SE+ M AQ+V Q + ++ + + Sbjct: 383 VAASREVSGAMDDVSGGASTLANSVDEVSEQLENM---AQSVEQMNNSVGEIKGVAGKAE 439 Query: 482 EG----------LIEISSKNEQEVQKISAITVKLQDSSNSISNITGAINQIAAQTNLLAL 531 E L+ ++ V + KL+ SI N+ I I+ QTNLLAL Sbjct: 440 EASKQGLNTMRNLVRTRGQSSSIVVHTEEASGKLEQRVGSIQNVVELIKGISDQTNLLAL 499 Query: 532 NASIEAARAGEHGRGFAVVASEIRKLAEQSSRQSNEIYEIIQQNLAFVAENNDSVMEINQ 591 NASIEAARAGE G+GFAVVA E+RKLAEQS + EI +I V + V ++ Sbjct: 500 NASIEAARAGEQGKGFAVVAEEVRKLAEQSKEATVEIATMIGDVQVEVRRVVEVVSKLKD 559 Query: 592 ISAQQDELVGQTQTAFQTILDKITEITVQIQSMADEIAHMQKDKDEVLDSAQSLXXXXXX 651 I+ Q+ + + + F+TI+ + I+ ++ + +E+ ++ ++ E+ ++ Sbjct: 560 IADIQNNVTTEAEAEFRTIMSVVNTISTSVEQIVEEVHNIGHEQGEITAVMHTIAGTSQE 619 Query: 652 XXXXXXXXXXXMMEQSSAVDQLAEMVGTIDQLSKSLAEAASKFRIE 697 Q + ++++A + ++ + + L +F+IE Sbjct: 620 SAAVSEEVNAATESQVNHLEKVAHTMESLTEHMRDLERLVEQFKIE 665 >ref|NP_847435.1| methyl-accepting chemotaxis protein [Bacillus anthracis str. Ames] gb|AAP28921.1| methyl-accepting chemotaxis protein [Bacillus anthracis str. Ames] Length = 666 Score = 164 bits (416), Expect = 5e-39, Method: Compositional matrix adjust. Identities = 138/523 (26%), Positives = 250/523 (47%), Gaps = 29/523 (5%) Query: 187 SNNKLLLVFSQPVKSADGKVLGVLAMTVASDFFLDKLGNININAKGKIEVLSRGGI-VLY 245 + KL + S+ + DG VLGV+AM V+ L NI N G++ +++ I ++Y Sbjct: 160 ATGKLSIGVSKAIVGQDGSVLGVVAMDVSLGTIQKLLHNIQYNNGGEMFIVNDKNIAIVY 219 Query: 246 NSSDPARIGQDVGDDPGIKPLLETRATDELEIQTTELGTE----YLRINKIPKADLTISV 301 P ++G+DV +P LL++ DE + T LG + Y ++ K I + Sbjct: 220 ----PEKVGKDVSKEP----LLKSLNKDETKFAVTTLGGKDVVAYSQL--FDKMKWKIGI 269 Query: 302 IDSYDDIERPIQDMLMKILIVTVIALLVAIGFGLLLSRSITKPVVTLTSRFKTLATGDLT 361 + + I+ + ++I+ I+L V I L SR + +P+ LT+ + +A GDLT Sbjct: 270 VYPKETIDGLLNSTRNTVIIMACISLFVGIVASYLFSRRLARPLQLLTNHVQKVAEGDLT 329 Query: 362 VTADGSYKSEFKDLADSFNTMVQQNKALITNMNDSITILKTSTHELEETSKQAARSVNET 421 + + K E +L + FN MV+Q +++ + +S++ ++ ST+ L + NET Sbjct: 330 LQMKVTSKDEVGELTNHFNHMVEQMNEMVSKIKNSVSTVQRSTNNLHYLT-------NET 382 Query: 422 SVTSMGIAQAMELQSNDTEQIVNKFYGFSEKFAGMNDNAQAVRQRAEEITQVFHTSNQ-- 479 S ++ AM+ S + N SE+ M + + + EI V + + Sbjct: 383 VAASKEVSGAMDDVSGGASTLANSVDEVSEQLENMIQSVEQMNSSVGEIKGVAGKAEEAS 442 Query: 480 -----VVEGLIEISSKNEQEVQKISAITVKLQDSSNSISNITGAINQIAAQTNLLALNAS 534 + L+ ++ V + KL+ SI N+ I I+ QTNLLALNAS Sbjct: 443 KQGLNTMRNLVRTREQSSSIVVHTEEASGKLEQRVGSIQNVVELIKGISDQTNLLALNAS 502 Query: 535 IEAARAGEHGRGFAVVASEIRKLAEQSSRQSNEIYEIIQQNLAFVAENNDSVMEINQISA 594 IEAARAGE G+GFAVVA E+RKLAEQS + EI +I V + V ++ I+ Sbjct: 503 IEAARAGEQGKGFAVVAEEVRKLAEQSKEATVEIATMIGDVQVEVRRVVEVVSKLKDIAD 562 Query: 595 QQDELVGQTQTAFQTILDKITEITVQIQSMADEIAHMQKDKDEVLDSAQSLXXXXXXXXX 654 Q+ + + + F+TI+ + I+ ++ + +E+ ++ ++ E+ ++ Sbjct: 563 IQNNVTTEAEAEFRTIMSVVNIISTSVEQIVEEVHNIGHEQGEITAVMHTIAGTSQESAA 622 Query: 655 XXXXXXXXMMEQSSAVDQLAEMVGTIDQLSKSLAEAASKFRIE 697 Q + ++++A + ++ + + L +F+IE Sbjct: 623 VSEEVNAATESQVNHLEKVAHTMESLTEHMRDLERLVEQFKIE 665 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.312 0.127 0.330 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 806,726,638 Number of extensions: 29981531 Number of successful extensions: 103444 Number of sequences better than 1.0e-05: 2024 Number of HSP's gapped: 100233 Number of HSP's successfully gapped: 2075 Length of query: 697 Length of database: 757,237,578 Length adjustment: 133 Effective length of query: 564 Effective length of database: 396,129,411 Effective search space: 223416987804 Effective search space used: 223416987804 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 130 (54.7 bits)