BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0254.0013.p (400 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00061927.1| COG0787: Alanine racemase [Clostridium thermo... 340 2e-92 ref|NP_844479.1| alanine racemase [Bacillus anthracis str. Ames]... 335 1e-90 ref|NP_655935.1| Ala_racemase, Alanine racemase [Bacillus anthra... 333 4e-90 ref|NP_978476.1| alanine racemase [Bacillus cereus ATCC 10987] >... 332 1e-89 ref|NP_694148.1| D-alanine racemase [Oceanobacillus iheyensis HT... 328 1e-88 >ref|ZP_00061927.1| COG0787: Alanine racemase [Clostridium thermocellum ATCC 27405] Length = 391 Score = 340 bits (873), Expect = 2e-92, Method: Compositional matrix adjust. Identities = 168/383 (43%), Positives = 247/383 (64%), Gaps = 4/383 (1%) Query: 17 AEINLDDLRANYEAFRKALPAETKFMGCVKGNAYGHGAVEVTRELERLGADYVSVAFLDE 76 AE+NLD++ N + R+ + + + MG VK +AYGHG +E+ R L G ++V+ LDE Sbjct: 12 AEVNLDNIAHNVKEIRRIVDKKVEIMGVVKADAYGHGVMEIARTLLENGVTRLAVSMLDE 71 Query: 77 ALELRQAGILIPILVLGYTSPEGIPVAWKNHVTVTLFTPEVLEAVRQLPVDPEHRLKVHI 136 A++LRQ GI +PIL+L YT P N VT T+F+ ++ EA+ + V +K+HI Sbjct: 72 AIQLRQNGIKVPILILSYTDPVRAEEIVLNDVTQTVFSHDLAEALSEAAVKHHRNVKIHI 131 Query: 137 KIDSGMGRLGLLPA-DAPDFIAEVHSVAQAELEGMFTHFAKADESNKSYTLMQYRRFMSV 195 KID+GM R+G +P A + ++ + +EG+FTHFA ADE++KSYT MQ+ RFMS+ Sbjct: 132 KIDTGMTRVGFMPGYSAVKNVVQISKLPGIIIEGLFTHFASADEADKSYTYMQFERFMSI 191 Query: 196 AETLRDRDIHIPIIHTGNSATAIDTPLLSSNMVRVGISLYGFYPSTEVNRELVALHPVMT 255 L ++IP+ H NSA I+ P + NMVR GI+LYG YPS EV++ + L P M+ Sbjct: 192 VGELNRIGVYIPVKHVCNSAALIEFPEMHLNMVRPGIALYGLYPSDEVDKTKIDLRPAMS 251 Query: 256 LKTQAVYVKTLPPDWGISYGTRYFTDSEEIIATLPVGYADGYSRMLTGKAEVLIRGRRVP 315 LK + VK + D ISYG + T IAT+P+GYADGY+R+LTGK +VL+ G+ P Sbjct: 252 LKANVILVKDVEKDTFISYGRIFKTSRNSRIATIPIGYADGYTRLLTGKGKVLLNGQLAP 311 Query: 316 VVGTICMDQCMVTLKSFAEEAEQIKAGEEVVLIGRQGAVSITADELALHLGTIHYEVICM 375 +VG ICMDQCMV + + +K G+E VLIG+Q I ++LA +GTI+YE++ + Sbjct: 312 IVGRICMDQCMVDITDIEGD---VKVGDEAVLIGKQKDNEIKVEDLAKSVGTINYELVSI 368 Query: 376 LAHRVPRVYVREGAAPNLVNPLL 398 + R+PRVY++EG N++N L+ Sbjct: 369 IGKRIPRVYLKEGKIYNVLNYLI 391 >ref|NP_844479.1| alanine racemase [Bacillus anthracis str. Ames] gb|AAP25965.1| alanine racemase [Bacillus anthracis str. Ames] Length = 391 Score = 335 bits (858), Expect = 1e-90, Method: Compositional matrix adjust. Identities = 165/386 (42%), Positives = 243/386 (62%), Gaps = 5/386 (1%) Query: 13 RPTVAEINLDDLRANYEAFRKALPAET-KFMGCVKGNAYGHGAVEVTRELERLGADYVSV 71 R T+ E++L+ ++ N + F+K + E M VK N YGHGAVEV + G + +++ Sbjct: 7 RDTIVEVDLNAVKHNVKEFKKRVNDENIAMMAAVKANGYGHGAVEVAKAAIEAGINQLAI 66 Query: 72 AFLDEALELRQAGILIPILVLGYTSPEGIPVAWKNHVTVTLFTPEVLEAVRQLPVDPEHR 131 AF+DEA+ELR+AGI +PIL+LGYTS A + V +T++ E L+ + ++ + + Sbjct: 67 AFVDEAIELREAGINVPILILGYTSVAAAEEAIQYDVMMTVYRSEDLQGINEIANRLQKK 126 Query: 132 LKVHIKIDSGMGRLGLLPADAPDFIAEVHSVAQAELEGMFTHFAKADESNKSYTLMQYRR 191 ++ +KID+GM R+GL + F+ E+ + E+ GMFTH++ ADE +KSYT MQ Sbjct: 127 AQIQVKIDTGMSRIGLQEEEVKPFLEELKRMEYVEVVGMFTHYSTADEIDKSYTNMQTSL 186 Query: 192 FMSVAETLRDRDIHIPIIHTGNSATAIDTPLLSSNMVRVGISLYGFYPSTEVNRELVALH 251 F T ++ IHIP IH+ NSA +++ NMVRVGI +YG YPS EVN +V+L Sbjct: 187 FEKAVNTAKELGIHIPYIHSSNSAGSMEPSNTFQNMVRVGIGIYGMYPSKEVNHSVVSLQ 246 Query: 252 PVMTLKTQAVYVKTLPPDWGISYGTRYFTDSEEIIATLPVGYADGYSRMLTGKAEVLIRG 311 P ++LK++ ++K + G+SYG Y T EE IAT+P+GYADGY+R L+ K LI G Sbjct: 247 PALSLKSKVAHIKHAKKNRGVSYGNTYVTTGEEWIATVPIGYADGYNRQLSNKGHALING 306 Query: 312 RRVPVVGTICMDQCMVTLKSFAEEAEQIKAGEEVVLIGRQGAVSITADELALHLGTIHYE 371 RVPV+G +CMDQ M+ + +A ++ G+EVV G+QG +I +E+A LGTI+YE Sbjct: 307 VRVPVIGRVCMDQLMLDVS----KAMPVQVGDEVVFYGKQGEENIAVEEIADMLGTINYE 362 Query: 372 VICMLAHRVPRVYVREGAAPNLVNPL 397 V CML R+PRVY +VN L Sbjct: 363 VTCMLDRRIPRVYKENNETTAVVNIL 388 >ref|NP_655935.1| Ala_racemase, Alanine racemase [Bacillus anthracis A2012] Length = 408 Score = 333 bits (854), Expect = 4e-90, Method: Compositional matrix adjust. Identities = 165/389 (42%), Positives = 243/389 (62%), Gaps = 5/389 (1%) Query: 13 RPTVAEINLDDLRANYEAFRKALPAET-KFMGCVKGNAYGHGAVEVTRELERLGADYVSV 71 R T+ E++L+ ++ N + F+K + E M VK N YGHGAVEV + G + +++ Sbjct: 24 RDTIVEVDLNAVKHNVKEFKKRVNDENIAMMAAVKANGYGHGAVEVAKAAIEAGINQLAI 83 Query: 72 AFLDEALELRQAGILIPILVLGYTSPEGIPVAWKNHVTVTLFTPEVLEAVRQLPVDPEHR 131 AF+DEA+ELR+AGI +PIL+LGYTS A + V +T++ E L+ + ++ + Sbjct: 84 AFVDEAIELREAGINVPILILGYTSVAAAEEAIQYDVMMTVYRSEDLQGINEIANRLXKK 143 Query: 132 LKVHIKIDSGMGRLGLLPADAPDFIAEVHSVAQAELEGMFTHFAKADESNKSYTLMQYRR 191 ++ +KID+GM R+GL + F+ E+ + E+ GMFTH++ ADE +KSYT MQ Sbjct: 144 AQIQVKIDTGMSRIGLQEEEVKPFLEELKRMEYVEVVGMFTHYSTADEIDKSYTNMQTSL 203 Query: 192 FMSVAETLRDRDIHIPIIHTGNSATAIDTPLLSSNMVRVGISLYGFYPSTEVNRELVALH 251 F T ++ IHIP IH+ NSA +++ NMVRVGI +YG YPS EVN +V+L Sbjct: 204 FEKAVNTAKELGIHIPYIHSSNSAGSMEPSNTFQNMVRVGIGIYGMYPSKEVNHSVVSLQ 263 Query: 252 PVMTLKTQAVYVKTLPPDWGISYGTRYFTDSEEIIATLPVGYADGYSRMLTGKAEVLIRG 311 P ++LK++ ++K + G+SYG Y T EE IAT+P+GYADGY+R L+ K LI G Sbjct: 264 PALSLKSKVAHIKHAKKNRGVSYGNTYVTTGEEWIATVPIGYADGYNRQLSNKGHALING 323 Query: 312 RRVPVVGTICMDQCMVTLKSFAEEAEQIKAGEEVVLIGRQGAVSITADELALHLGTIHYE 371 RVPV+G +CMDQ M+ + +A ++ G+EVV G+QG +I +E+A LGTI+YE Sbjct: 324 VRVPVIGRVCMDQLMLDVS----KAMPVQVGDEVVFYGKQGEENIAVEEIADMLGTINYE 379 Query: 372 VICMLAHRVPRVYVREGAAPNLVNPLLQT 400 V CML R+PRVY +VN L + Sbjct: 380 VTCMLDRRIPRVYKENNETTAVVNILRKN 408 >ref|NP_978476.1| alanine racemase [Bacillus cereus ATCC 10987] gb|AAS41084.1| alanine racemase [Bacillus cereus ATCC 10987] Length = 391 Score = 332 bits (850), Expect = 1e-89, Method: Compositional matrix adjust. Identities = 164/386 (42%), Positives = 242/386 (62%), Gaps = 5/386 (1%) Query: 13 RPTVAEINLDDLRANYEAFRKALPAET-KFMGCVKGNAYGHGAVEVTRELERLGADYVSV 71 R T+ E++L+ ++ N + F+K + E M VK N YGHGAVEV + G + ++V Sbjct: 7 RDTIVEVDLNAVKHNVKEFKKRVNDENIAMMAAVKANGYGHGAVEVAKAAIEAGINQLAV 66 Query: 72 AFLDEALELRQAGILIPILVLGYTSPEGIPVAWKNHVTVTLFTPEVLEAVRQLPVDPEHR 131 AF+DEA+ELR+AGI +PIL+LGYTS A + V +T++ E L+ + ++ + + Sbjct: 67 AFVDEAIELREAGINVPILILGYTSVAAAEEAIQYDVMMTVYRSEDLQGINEIANRLQKK 126 Query: 132 LKVHIKIDSGMGRLGLLPADAPDFIAEVHSVAQAELEGMFTHFAKADESNKSYTLMQYRR 191 ++ +KID+GM R+GL + F+ E+ + E+ GMFTH++ ADE +KSYT MQ Sbjct: 127 AQIQVKIDTGMSRIGLQEEEVKPFLEELKRMEYVEVVGMFTHYSTADEIDKSYTNMQTSL 186 Query: 192 FMSVAETLRDRDIHIPIIHTGNSATAIDTPLLSSNMVRVGISLYGFYPSTEVNRELVALH 251 F ++ IHIP IH+ NSA +++ NMVRVGI +YG YPS EV+ +V+L Sbjct: 187 FEKAVNAAKELGIHIPYIHSSNSAGSMELSNTFQNMVRVGIGIYGMYPSKEVDHSVVSLQ 246 Query: 252 PVMTLKTQAVYVKTLPPDWGISYGTRYFTDSEEIIATLPVGYADGYSRMLTGKAEVLIRG 311 P ++LK++ ++K + G+SYG Y T EE IAT+P+GYADGY+R L+ K LI G Sbjct: 247 PALSLKSKVAHIKHAKKNRGVSYGNTYVTTGEEWIATVPIGYADGYNRQLSNKGYALING 306 Query: 312 RRVPVVGTICMDQCMVTLKSFAEEAEQIKAGEEVVLIGRQGAVSITADELALHLGTIHYE 371 RVPV+G +CMDQ M+ + +A ++ G+EVV G+QG +I +E+A LGTI+YE Sbjct: 307 VRVPVIGRVCMDQLMLDVS----KAMPVQVGDEVVFYGKQGEENIAVEEIADMLGTINYE 362 Query: 372 VICMLAHRVPRVYVREGAAPNLVNPL 397 V CML R+PRVY +VN L Sbjct: 363 VTCMLDRRIPRVYKENNETTAIVNIL 388 >ref|NP_694148.1| D-alanine racemase [Oceanobacillus iheyensis HTE831] dbj|BAC15182.1| D-alanine racemase [Oceanobacillus iheyensis HTE831] Length = 392 Score = 328 bits (841), Expect = 1e-88, Method: Compositional matrix adjust. Identities = 165/385 (42%), Positives = 240/385 (62%), Gaps = 6/385 (1%) Query: 8 VQASYRPTVAEINLDDLRANYEAFRKALPAETKFMGCVKGNAYGHGAVEVTRELERLGAD 67 +Q SYR T E++LD + N AF+ + TK M VK + YGHGAVEV +E GAD Sbjct: 2 IQGSYRDTWTEVSLDAIHHNVTAFKNHIDHHTKLMAVVKADGYGHGAVEVAQEALTAGAD 61 Query: 68 YVSVAFLDEALELRQAGILIPILVLGYTSPEGIPVAWKNHVTVTLFTPEVLEAVRQLPVD 127 Y++VA LDEA++LR AGI P+LVLGYT P+G+ A + +T+T+FT E E V+ Sbjct: 62 YLAVAILDEAIQLRDAGIDAPLLVLGYTHPDGLKTAIEQQITLTVFTKEDAEQVKIAAEL 121 Query: 128 PEHRLKVHIKIDSGMGRLGLLPADAPDFIAEVHSVAQAELEGMFTHFAKADESNKSYTLM 187 + ++H+KI+SGM R+G+ + IA+ + LEG FTHFA AD ++ +YT M Sbjct: 122 LQKTARIHLKIESGMNRIGIATKEEAVEIAKALHSSFVMLEGAFTHFADADNTDPTYTEM 181 Query: 188 QYRRFMSVAETLRDRDIHIPIIHTGNSATAIDTPLLSSNMVRVGISLYGFYPSTEVNREL 247 Q+ RF + LR HIPI+H N+A I P + +MVRVGI +YG YP T + +EL Sbjct: 182 QFHRFNQIISHLRTH-CHIPIVHCCNTAATIAYPDMHLDMVRVGIGIYGLYPETHL-KEL 239 Query: 248 VALHPVMTLKTQAVYVKTLPPDWGISYGTRYFTDSEEIIATLPVGYADGYSRMLTGKAEV 307 + L M+LKT+ VY+KT+ D ISYG + T+ + IAT+P+GYADG+SR+L+ + +V Sbjct: 240 IDLKQAMSLKTKPVYIKTVDQDTAISYGLTFTTERKSTIATMPIGYADGFSRLLSNRGDV 299 Query: 308 LIRGRRVPVVGTICMDQCMVTLKSFAEEAEQIKAGEEVVLIGRQGAVSITADELALHLGT 367 ++ R P+VG ICMDQ M+ + + E + + + + G I +E+A +GT Sbjct: 300 IVHAGRAPIVGRICMDQSMIDVT----DVENVGLDDVITIFGEPTEGYIAMEEVANLMGT 355 Query: 368 IHYEVICMLAHRVPRVYVREGAAPN 392 IHYE C++ RVPR+Y+R+ A N Sbjct: 356 IHYETACLIGKRVPRIYMRQSEAVN 380 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.320 0.137 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 579,607,927 Number of extensions: 23847516 Number of successful extensions: 56024 Number of sequences better than 1.0e-05: 498 Number of HSP's gapped: 54696 Number of HSP's successfully gapped: 500 Length of query: 400 Length of database: 757,237,578 Length adjustment: 128 Effective length of query: 272 Effective length of database: 409,704,906 Effective search space: 111439734432 Effective search space used: 111439734432 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 127 (53.5 bits)