BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0251.0034.p (312 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_349519.1| acetyl esterase family enzyme [Clostridium acet... 167 2e-40 ref|NP_721071.1| putative esterase [Streptococcus mutans UA159] ... 158 2e-37 ref|ZP_00121135.1| COG0657: Esterase/lipase [Bifidobacterium lon... 154 3e-36 ref|NP_695867.1| protein probably involved in xylan degradation;... 152 1e-35 ref|NP_784706.1| lipase/esterase (putative) [Lactobacillus plant... 137 3e-31 >ref|NP_349519.1| acetyl esterase family enzyme [Clostridium acetobutylicum] pir||H97258 acetyl esterase family enzyme [imported] - Clostridium acetobutylicum gb|AAK80859.1| acetyl esterase family enzyme [Clostridium acetobutylicum] Length = 272 Score = 167 bits (424), Expect = 2e-40, Method: Compositional matrix adjust. Identities = 103/290 (35%), Positives = 150/290 (51%), Gaps = 49/290 (16%) Query: 18 MEAYILHNSVEFNHNLKRPVALIIPGGAYISTSDREAEQVALAFATHGYHAFVLRYSIGK 77 ME YIL N + KR LI+PGG Y TS REAE +A+ + G+ FVL YS+ Sbjct: 26 METYIL------NGDKKRSAILILPGGGYNHTSSREAEPIAVNYNAAGFSTFVLHYSVAP 79 Query: 78 EALMPQPILEGFKAISIIRDHADEWCVDVNRIVPCGFSAGGHLAASLATMWNLPEFANAL 137 PQP+L+ +AISIIR++ADEW +D ++I CGFSAGGHLA L W E N Sbjct: 80 NR-YPQPLLDAARAISIIRENADEWNIDKDKIAVCGFSAGGHLAGLLGVNWFKEELFNVD 138 Query: 138 GCSSESIKPNAVVLSYPCVVVPNHAGSLDTGMPAEALEAFKANPMMSELNEALVVKDGTI 197 G S E +KPNA++L YP + A ++FK L+ +G Sbjct: 139 GISKEFLKPNALILCYPVITSGKFAHK----------KSFK----------CLLGDEGKK 178 Query: 198 RLDLIDVMYRNMTGKPDWKAEDLQSYSTDLLVSEDTVPSFVWATENDILVPVSGAIQFVQ 257 LD + + +++TGK T +F+W T ND +VPV ++ F + Sbjct: 179 LLDEVSIE-KHVTGK--------------------TPTTFIWHTFNDKVVPVQNSMLFTE 217 Query: 258 AMLKNGRRIEFHLFAGGEHGLSLAKATTAGSPNMVNDEVAVWFNMAIAFL 307 A+ K E H++ G+HG++L T G N +N +A WFN+++ ++ Sbjct: 218 ALSKVEVSFEMHIYPEGKHGMALGTKETDGGGN-INPHLASWFNLSVEWI 266 >ref|NP_721071.1| putative esterase [Streptococcus mutans UA159] gb|AAN58377.1| putative esterase [Streptococcus mutans UA159] Length = 297 Score = 158 bits (399), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 89/299 (29%), Positives = 151/299 (50%), Gaps = 18/299 (6%) Query: 13 KEEVFMEAYILHNSVEFNHNLKRPVALIIPGGAYISTSDREAEQVALAFATHGYHAFVLR 72 K EV Y++ + + + RP +I GG + +DRE E VAL F G+ A L Sbjct: 14 KSEVTYTTYLIEKTPDIKNKNPRPAVIICGGGGFFKVTDREKEPVALYFLNKGFQAITLD 73 Query: 73 YSIGKEALMPQPILEGFKAISIIRDHADEWCVDVNRIVPCGFSAGGHLAASLATMWNLPE 132 Y++GK + P+P+ + K + I+R++A W +D +++ GFSAG +ASLA WN P Sbjct: 74 YTVGKGSGYPKPLYDLAKMLLIVRENAQNWNIDTEKVIFIGFSAGATHSASLANSWNEPF 133 Query: 133 FANALGCSSESIKPNAVVLSYPCVVVPNHAGSLDTGMPAEALEAFKANPMMSELNEALVV 192 E++KP+ V+LSYP LD E + N M ++L+ Sbjct: 134 LQKYFDYPGETLKPSLVILSYPL---------LDFNYQYEQVSVDPDNQMPTKLSPM--- 181 Query: 193 KDGTIRLDLIDVMYRNMTGKPDWKAEDLQSYSTDLLVSEDTVPSFVWATENDILVPVSGA 252 ++D + + + GK + E+L+ YS +S TVP+F+W ++D + + Sbjct: 182 ----PKIDFLTGALKTVVGK-ELTTENLKEYSPIEHISTATVPTFIWGMQDDDCIYNNAL 236 Query: 253 IQFVQAMLKNGRRIEFHLFAGGEHGLSLAKATTAGSPNMVNDEVAVWFNMAIAFLAKTL 311 +++ + + NG E H+FA GEHGLS+ + T + +E+ +W + I F+ + L Sbjct: 237 LEYAKRLKDNGILYELHMFALGEHGLSVINSNTQAKFSDY-EELGIWKKLCIQFINRVL 294 >ref|ZP_00121135.1| COG0657: Esterase/lipase [Bifidobacterium longum DJO10A] Length = 290 Score = 154 bits (388), Expect = 3e-36, Method: Compositional matrix adjust. Identities = 106/313 (33%), Positives = 150/313 (47%), Gaps = 46/313 (14%) Query: 1 MMICKKIPICENKEEVFMEAYILHNSVEFNHNLKRPVALIIPGGAYISTSDREAEQVALA 60 M ++ + + E Y+ NS E + RP LI+PGG Y TSDREAE VAL Sbjct: 18 MQYIQQTIVGIDGSEARFFGYVADNSEEMEPDRIRPAILILPGGGYAMTSDREAEPVALQ 77 Query: 61 FATHGYHAFVLRYSIGKEALMPQPILEGFKAISIIRDHADEWCVDVNRIVPCGFSAGGHL 120 F G+ FVLRYS+ + + P +LE +A+ +IR +AD+W V+ ++ GFSAGGHL Sbjct: 78 FLAKGFAVFVLRYSV-QPSRYPVALLEAAEAMRLIRANADQWHVNPAQVAVLGFSAGGHL 136 Query: 121 AASLATMWNLPEFANALGCSSESIKPNAVVLSYPCVVVPN--HAGSLDTGMPAEALEAFK 178 AA+LAT + G ++++PNA++LSYP + H GS + +A Sbjct: 137 AANLATSVGDEDIREQGGMDPDAVRPNALMLSYPVITAGKYAHRGSFQCLLGDQA----- 191 Query: 179 ANPMMSELNEALVVKDGTIRLDLIDVMYRNMTGKPDWKAEDLQSYSTDLLVSEDTVPSFV 238 N+AL L +S + + T P FV Sbjct: 192 -------HNQAL-----------------------------LDKFSIEKHIDAKTPPVFV 215 Query: 239 WATENDILVPVSGAIQFVQAMLKNGRRIEFHLFAGGEHGLSLAKATTAGSPNMVN--DEV 296 W T D VPV + +QA G IE HLF G HGLSLA A TAG+ + + V Sbjct: 216 WHTMTDDAVPVENTLMLIQACRAAGVSIEAHLFPEGSHGLSLANAETAGNGFYAHIVECV 275 Query: 297 AVWFNMAIAFLAK 309 W ++A A+L + Sbjct: 276 QCWPDLAEAWLRR 288 >ref|NP_695867.1| protein probably involved in xylan degradation; possible xylan esterase [Bifidobacterium longum NCC2705] gb|AAN24503.1| protein probably involved in xylan degradation; possible xylan esterase [Bifidobacterium longum NCC2705] Length = 298 Score = 152 bits (383), Expect = 1e-35, Method: Compositional matrix adjust. Identities = 105/313 (33%), Positives = 149/313 (47%), Gaps = 46/313 (14%) Query: 1 MMICKKIPICENKEEVFMEAYILHNSVEFNHNLKRPVALIIPGGAYISTSDREAEQVALA 60 M ++ + + E Y+ NS E + RP LI+PGG Y TSDREAE VAL Sbjct: 26 MQYIQQTIVGIDGSEARFFGYVADNSEEMEPDRIRPAILILPGGGYAMTSDREAEPVALQ 85 Query: 61 FATHGYHAFVLRYSIGKEALMPQPILEGFKAISIIRDHADEWCVDVNRIVPCGFSAGGHL 120 F G+ FVLRYS+ + + P +LE +A+ +IR + D+W V+ ++ GFSAGGHL Sbjct: 86 FLAKGFAVFVLRYSV-QPSRYPVALLEAAEAMRLIRANVDQWHVNPAQVAVLGFSAGGHL 144 Query: 121 AASLATMWNLPEFANALGCSSESIKPNAVVLSYPCVVVPN--HAGSLDTGMPAEALEAFK 178 AA+LAT + G ++++PNA++LSYP + H GS + +A Sbjct: 145 AANLATSVGDEDIREQGGMDPDAVRPNALMLSYPVITAGKYAHRGSFQCLLGDQA----- 199 Query: 179 ANPMMSELNEALVVKDGTIRLDLIDVMYRNMTGKPDWKAEDLQSYSTDLLVSEDTVPSFV 238 N+AL L +S + + T P FV Sbjct: 200 -------HNQAL-----------------------------LDKFSIEKHIDAKTPPVFV 223 Query: 239 WATENDILVPVSGAIQFVQAMLKNGRRIEFHLFAGGEHGLSLAKATTAGSPNMVN--DEV 296 W T D VPV + +QA G IE HLF G HGLSLA A TAG+ + + V Sbjct: 224 WHTMTDDAVPVENTLMLIQACRAAGVSIEAHLFPEGSHGLSLANAETAGNGFYAHIVECV 283 Query: 297 AVWFNMAIAFLAK 309 W ++A A+L + Sbjct: 284 QCWPDLAEAWLRR 296 >ref|NP_784706.1| lipase/esterase (putative) [Lactobacillus plantarum WCFS1] emb|CAD63553.1| lipase/esterase (putative) [Lactobacillus plantarum WCFS1] Length = 264 Score = 137 bits (345), Expect = 3e-31, Method: Compositional matrix adjust. Identities = 88/288 (30%), Positives = 129/288 (44%), Gaps = 47/288 (16%) Query: 23 LHNSVEFNHNLKRPVALIIPGGAYISTSDREAEQVALAFATHGYHAFVLRYSI--GKEAL 80 LH H P +I+PGG+Y +AE +A+AFA HGY AF L Y++ ++ L Sbjct: 19 LHQPDTNAHQTNLPAIIIVPGGSYTHIPVAQAESLAMAFAGHGYQAFYLEYTLLTDQQPL 78 Query: 81 MPQPILEGFKAISIIRDHADEWCVDVNRIVPCGFSAGGHLAASLATMWNLPEFANALGCS 140 P+L+ +A++++R HA EW +D +I P GFS GGH+ A W A L + Sbjct: 79 GLAPVLDLGRAVNLLRQHAAEWHIDPQQITPAGFSVGGHIVALYNDYWAT-RVATELNVT 137 Query: 141 SESIKPNAVVLSYPCVVVPNHAGSLDTGMPAEALEAFKANPMMSELNEALVVKDGTIRLD 200 +KPN VVL YP + S G P + P +EL Sbjct: 138 PAMLKPNNVVLGYPVI-------SPLLGFPKDDATLATWTPTPNEL-------------- 176 Query: 201 LIDVMYRNMTGKPDWKAEDLQSYSTDLLVSEDTVPSFVWATENDILVPVSGAIQFVQAML 260 + D V+ D P+F+W T +D +VP + + + A+ Sbjct: 177 -----------------------AADQHVNSDNQPTFIWTTADDPIVPATNTLAYATALA 213 Query: 261 KNGRRIEFHLFAGGEHGLSLAKATTAGSPNMVNDEVAVWFNMAIAFLA 308 E H+F G HGL+LA A TA P+ VA W +A+ +LA Sbjct: 214 TAKIPYELHVFKHGPHGLALANAQTAWKPDANQPHVAHWLTLALEWLA 261 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.320 0.134 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 459,869,945 Number of extensions: 18457739 Number of successful extensions: 40301 Number of sequences better than 1.0e-05: 49 Number of HSP's gapped: 40323 Number of HSP's successfully gapped: 58 Length of query: 312 Length of database: 757,237,578 Length adjustment: 125 Effective length of query: 187 Effective length of database: 417,850,203 Effective search space: 78137987961 Effective search space used: 78137987961 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 126 (53.1 bits)