BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0251.0030.p (496 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00061173.1| COG3250: Beta-galactosidase/beta-glucuronidas... 293 1e-77 ref|NP_625069.1| putative secreted beta-galactosidase [Streptomy... 256 1e-66 ref|NP_828655.1| putative beta-galactosidase precursor [Streptom... 254 3e-66 ref|NP_562182.1| beta-galactosidase [Clostridium perfringens] >g... 253 7e-66 emb|CAC14566.2| beta-galactosidase [Bifidobacterium bifidum] 245 2e-63 >ref|ZP_00061173.1| COG3250: Beta-galactosidase/beta-glucuronidase [Clostridium thermocellum ATCC 27405] Length = 941 Score = 293 bits (749), Expect = 1e-77, Method: Compositional matrix adjust. Identities = 147/359 (40%), Positives = 218/359 (60%), Gaps = 6/359 (1%) Query: 54 KKFYVPEEYKDKRVTFEFEGVYMHAMVYINGDFAGQHPFGYTNFYIKADRFLKYGAENEI 113 K F VP +Y K+V +F+G YM++ V+ING + G P+GY++F +L G +N I Sbjct: 31 KTFTVPSDYSGKKVFIQFDGAYMNSQVWINGTYLGIRPYGYSSFEYDLTPYLNIGGKNVI 90 Query: 114 KV-VAKSHDDSRWYTGTGIYRNTKIMVADLVHIAVDGVKINATDIESKHAVVVVATDVEN 172 V + + +SRWY+G+GIYRN + V D VH+ G+ I ++ A V+T V N Sbjct: 91 AVKINNNQPNSRWYSGSGIYRNVWLTVLDPVHVDYCGMFITTPNVSRDSATANVSTKVVN 150 Query: 173 EGINPRTVRIVTEIVDADGNSVASDTAPFTAF-AGEKATSRQRLYIREPKLWNVETPYLY 231 +G + +TV + T I+DA+GN VASDT+ AG T Q L + P LW+ ++PYLY Sbjct: 151 QGNSEKTVSLKTIIMDANGNQVASDTSSAVNISAGSDYTFNQNLTVSNPNLWSPDSPYLY 210 Query: 232 TCISKVMDGEHVLDEETHTFGIRTLSLDAEDGLRINGEVVKLRGACIHHDNGVIGASTIE 291 ++V+ V D T G R L+ + G +NG K++G C+HHD G +GA+ Sbjct: 211 MVQTQVIVDGKVADTYKSTMGFRYLNFSSTTGFSLNGVKTKIKGVCMHHDLGALGAAVNY 270 Query: 292 RADERRIEILKQAGFNAIRSAHHPASKALLKACDRIGMLVMDESFDIWTHHKSDYDYALH 351 RA ER+++I+K+ G NAIR+AH+P +L+ CDR+G++VMDE+FD W K+ DY L+ Sbjct: 271 RAIERQLQIMKEMGCNAIRTAHNPPDPQVLEICDRLGLMVMDEAFDCWETGKTANDYHLY 330 Query: 352 FPTWWEQDIQAMVDKDYNHPSVILYSIGNEIPETGSANGTAWGRKIAEKIRSLDSTRYV 410 F W ++D+Q MV +D NHPSVI++SIGNEIP T K+ ++ +D TR V Sbjct: 331 FKDWAKRDLQDMVKRDRNHPSVIMWSIGNEIPNATVETAT----KLKNWVKEIDPTRPV 385 >ref|NP_625069.1| putative secreted beta-galactosidase [Streptomyces coelicolor A3(2)] emb|CAB61538.1| putative secreted beta-galactosidase [Streptomyces coelicolor A3(2)] Length = 1028 Score = 256 bits (653), Expect = 1e-66, Method: Compositional matrix adjust. Identities = 134/397 (33%), Positives = 217/397 (54%), Gaps = 5/397 (1%) Query: 18 KAVTLPHDAMIAKKRSAQAVSGSKKGYFSDGAYDYVKKFYVPEEYKDKRVTFEFEGVYMH 77 + V +PHD I + + + + S G+ G Y F +P KR++ EF+GVYM Sbjct: 76 REVAVPHDWSIEQTPTTEHGTTSGTGFLPGGLGWYRLAFTLPPALAGKRISVEFDGVYMD 135 Query: 78 AMVYINGDFAGQHPFGYTNFYIKADRFLKYGA--ENEIKVVAKSH-DDSRWYTGTGIYRN 134 + V+ NG G HP+GYT F + + EN + V ++ SRWY+G+GIYR Sbjct: 136 SRVHCNGREVGHHPYGYTGFALDLTDLVHTDGRTENVLAVRVRNQLPSSRWYSGSGIYRE 195 Query: 135 TKIMVADLVHIAVDGVKINATDIESKHAVVVVATDVENEGINPRTVRIVTEIVDADGNSV 194 +++V + VH+ G + + + AVV V T V N R V I + + DADG +V Sbjct: 196 ARLVVTEPVHVRRWGTYVTTPRVSGESAVVRVRTSVVNATGTAREVEIRSAVKDADGRTV 255 Query: 195 ASDTAPFTAFAGEKATSRQRLYIREPKLWNVETPYLYTCISKVMDGEHVLDEETHTFGIR 254 A + T + A+ L + P+LW+ +P+ YT +++ G +D FGIR Sbjct: 256 ARTAS--TVDVTDAASETHELTVARPRLWDFASPHRYTLHTELRVGGRTVDTYRTPFGIR 313 Query: 255 TLSLDAEDGLRINGEVVKLRGACIHHDNGVIGASTIERADERRIEILKQAGFNAIRSAHH 314 T +D E+G +NG K++G +HHD G +GA+ A R++ ++K G NA R++H+ Sbjct: 314 TFHVDPEEGFHLNGRYAKIKGVDLHHDLGALGAAVSADAVRRQMTLMKSMGVNAFRTSHN 373 Query: 315 PASKALLKACDRIGMLVMDESFDIWTHHKSDYDYALHFPTWWEQDIQAMVDKDYNHPSVI 374 P S +++ C+ +G++++ E+FD W K+ YDY F W E+D MV N P+V+ Sbjct: 374 PPSPEMIEVCEEMGIIMLVEAFDCWRTGKNRYDYGRFFDEWCERDATEMVLAARNSPAVV 433 Query: 375 LYSIGNEIPETGSANGTAWGRKIAEKIRSLDSTRYVM 411 ++SIGNE+P++ + G A +I E IR+ D TR ++ Sbjct: 434 MWSIGNEVPDSTATAGLAMADRIIEAIRAADDTRPLV 470 >ref|NP_828655.1| putative beta-galactosidase precursor [Streptomyces avermitilis MA-4680] dbj|BAC75190.1| putative beta-galactosidase precursor [Streptomyces avermitilis MA-4680] Length = 1030 Score = 254 bits (650), Expect = 3e-66, Method: Compositional matrix adjust. Identities = 138/397 (34%), Positives = 217/397 (54%), Gaps = 5/397 (1%) Query: 18 KAVTLPHDAMIAKKRSAQAVSGSKKGYFSDGAYDYVKKFYVPEEYKDKRVTFEFEGVYMH 77 + V +PHD I + + Q + S G+ G Y F +P KR++ EF+GVYM Sbjct: 78 REVAVPHDWSIEQTPTTQYGTTSGTGFLPGGLGWYRTAFTLPSALAGKRISVEFDGVYMD 137 Query: 78 AMVYINGDFAGQHPFGYTNFYIKADRFLKYGAENEIKVVAKSHD---DSRWYTGTGIYRN 134 + VY NG G+HP+GYT F L E + K + SRWY+G+GIYR Sbjct: 138 SYVYCNGTEVGRHPYGYTGFAFDLTDLLHTDGTTENVIAVKVQNRLPSSRWYSGSGIYRE 197 Query: 135 TKIMVADLVHIAVDGVKINATDIESKHAVVVVATDVENEGINPRTVRIVTEIVDADGNSV 194 +++V + VH+ G + DI + AVV V T V + V++V+ IV DG + Sbjct: 198 ARLVVTEPVHVRRWGTYVTTPDITEERAVVRVRTTVVDASGAGSEVQVVSRIVGPDGRTA 257 Query: 195 ASDTAPFTAFAGEKATSRQRLYIREPKLWNVETPYLYTCISKVMDGEHVLDEETHTFGIR 254 A T+ T ++ T L +++P+LW+ P+ YT +++ G D TFGIR Sbjct: 258 ARTTS--TVTVADQGTQTHELTVKKPQLWDFAHPHRYTLETELRVGGKRTDTHRTTFGIR 315 Query: 255 TLSLDAEDGLRINGEVVKLRGACIHHDNGVIGASTIERADERRIEILKQAGFNAIRSAHH 314 +++D ++G +NG K+RG +HHD G +GA+ A R++ I+K G NA R++H+ Sbjct: 316 RVTVDPDEGFHLNGTHAKIRGVDLHHDQGALGAAISIDAVRRQMTIMKSMGVNAFRTSHN 375 Query: 315 PASKALLKACDRIGMLVMDESFDIWTHHKSDYDYALHFPTWWEQDIQAMVDKDYNHPSVI 374 P S +++ C+ +G++++ E+FD W K+ YDY F W E+D MV N P+V+ Sbjct: 376 PPSPQMIEVCEELGIVMVVEAFDCWRTGKTRYDYGRFFDEWCEKDATEMVLAARNSPAVV 435 Query: 375 LYSIGNEIPETGSANGTAWGRKIAEKIRSLDSTRYVM 411 L+SIGNEIP++ S G A +I I++ D TR ++ Sbjct: 436 LWSIGNEIPDSTSTAGLAMADRIIGAIKAADDTRPLV 472 >ref|NP_562182.1| beta-galactosidase [Clostridium perfringens] dbj|BAB80972.1| beta-galactosidase [Clostridium perfringens str. 13] Length = 1355 Score = 253 bits (646), Expect = 7e-66, Method: Compositional matrix adjust. Identities = 148/421 (35%), Positives = 231/421 (54%), Gaps = 30/421 (7%) Query: 18 KAVTLPHDAMIAKKRSAQAVSGSKKGYFSDGAYDYVKKFYVPEEYKDKRVTFEFEGVYMH 77 + + LPHD I + + + S G+ G Y K F VPE+Y K T F+GVYM+ Sbjct: 78 RGLNLPHDWSIEGDFTVEGEAES--GFLLGGTGWYRKAFVVPEKYNSKDFTLNFDGVYMN 135 Query: 78 AMVYINGDFAGQHPFGYTNFYIKADRFLKYGAENEIKVVAKSHD---DSRWYTGTGIYRN 134 A VY+NG G+H +GYT+F L + E + K + SRWY+G+GIYR+ Sbjct: 136 AEVYVNGKKVGEHNYGYTSFAFDITEALICDGQTENIIAVKVSNPVPTSRWYSGSGIYRD 195 Query: 135 TKIMVADLVHIAVDGVKINATDIESKHAV---VVVATDVENEGINPRTVRIVTEIVDADG 191 + V D +H+A G + +E + V + T VENE + V + + +V++ G Sbjct: 196 VTLSVTDSIHVAHSGTTVTTPKLEEQKGGDVDVAIETIVENESKDNSMVTVKSTVVNSKG 255 Query: 192 NSVASDTAPFTAFA-GEKATSRQRLYIREPKLWNVETPYLYTCISKVMDGEHVLDEETHT 250 V+ + E T +Q + P LW+V+ P +Y S+V+ V+D Sbjct: 256 EEVSEAVINEQSIGVNESYTFKQTAIVNNPDLWSVDNPNMYKVKSEVLLDGKVIDTYFTD 315 Query: 251 FGIRTLSLDAEDGLRINGEVVKLRGACIHHDNGVIGASTIERADERRIEILKQAGFNAIR 310 FG R + D + G +NGE +KL+G C+HHD G +GA++ RA ER++E +K+ G NAIR Sbjct: 316 FGFRYYNFDKDTGFSLNGENMKLKGVCMHHDQGALGAASYYRAVERQMEKMKEMGVNAIR 375 Query: 311 SAHHPASKALLKACDRIGMLVMDESFDIWTHHKSD--YDYALHFP--------------- 353 +H+PAS+ LL+ C+R+G+LV++E+FD WT+ K+ D++ +F Sbjct: 376 VSHNPASEMLLEICNRLGLLVINEAFDTWTNPKNGNVNDFSKYFNEVIGEDNEILNGSPE 435 Query: 354 -TWWEQDIQAMVDKDYNHPSVILYSIGNEIPE--TGSA-NGTAWGRKIAEKIRSLDSTRY 409 TW E + ++MV N+PS+I++SIGNE+ E +GSA N T + I + I+ D TR+ Sbjct: 436 MTWGEFEARSMVKNSKNNPSIIMWSIGNEVLEGISGSASNYTNVAQNIIDWIKDEDETRH 495 Query: 410 V 410 V Sbjct: 496 V 496 >emb|CAC14566.2| beta-galactosidase [Bifidobacterium bifidum] Length = 1752 Score = 245 bits (625), Expect = 2e-63, Method: Compositional matrix adjust. Identities = 154/450 (34%), Positives = 235/450 (52%), Gaps = 33/450 (7%) Query: 18 KAVTLPHDAMIAKKRSAQAVSGSKKGYFSDGAYDYVKKFYVPEEYKDKRVTFEFEGVYMH 77 + V LPHD I +K S + ++ Y G Y K F + + KR+ F+GVYM+ Sbjct: 92 QQVDLPHDYSITQKYSQS--NEAESAYLPGGTGWYRKSFTIDRDLAGKRIAINFDGVYMN 149 Query: 78 AMVYINGDFAGQHPFGYTNFYIKADRFLKYGAENEIKV-VAKSHDDSRWYTGTGIYRNTK 136 A V+ NG G HP+GY+ F K+G EN I V V SRWY+G+GIYR+ Sbjct: 150 ATVWFNGVKLGTHPYGYSPFSFDLTGNAKFGGENTIVVKVENRLPSSRWYSGSGIYRDVT 209 Query: 137 IMVADLVHIAVDGVKINATDIESKHAVVV---VATDVENEGINPRTVRIVTEIVDADGNS 193 + V D VH+ +GV I + +++ V + T V N+ + + + G + Sbjct: 210 LTVTDGVHVGNNGVAIKTPSLATQNGGDVTMNLTTKVANDTEAAANITLKQTVFPKGGKT 269 Query: 194 VAS----DTAPFTAFAGEKATSRQRLYIREPKLWNVETPYLYTCISKVMDGEHVLDEETH 249 A+ TA + AG A + PKLW+++ P LYT ++V++G VLD Sbjct: 270 DAAIGTVTTASKSIAAGASADVTSTITAASPKLWSIKNPNLYTVRTEVLNGGKVLDTYDT 329 Query: 250 TFGIRTLSLDAEDGLRINGEVVKLRGACIHHDNGVIGASTIERADERRIEILKQAGFNAI 309 +G R DA G +NGE VKL+G +HHD G +GA RA ER++EIL++ G N+I Sbjct: 330 EYGFRWTGFDATSGFSLNGEKVKLKGVSMHHDQGSLGAVANRRAIERQVEILQKMGVNSI 389 Query: 310 RSAHHPASKALLKACDRIGMLVMDESFDIWTHHKSD--YDYALHF--------------- 352 R+ H+PA+KAL+ C+ G+LV++E FD+W K+ DY F Sbjct: 390 RTTHNPAAKALIDVCNEKGVLVVEEVFDMWNRSKNGNTEDYGKWFGQAIAGDNAVLGGDK 449 Query: 353 -PTWWEQDIQAMVDKDYNHPSVILYSIGNEIPE--TGSANG-TAWGRKIAEKIRSLDSTR 408 TW + D+ + +++D N PSVI++S+GNE+ E +GS +G A K+ ++ DSTR Sbjct: 450 DETWAKFDLTSTINRDRNAPSVIMWSLGNEMMEGISGSVSGFPATSAKLVAWTKAADSTR 509 Query: 409 YVMNSINGMVAVMNHLQEMFQGNNNSSDTG 438 + N + A N M G+N +++ G Sbjct: 510 PMTYGDNKIKANWNESNTM--GDNLTANGG 537 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.134 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 740,035,295 Number of extensions: 31059580 Number of successful extensions: 72719 Number of sequences better than 1.0e-05: 335 Number of HSP's gapped: 72082 Number of HSP's successfully gapped: 341 Length of query: 496 Length of database: 757,237,578 Length adjustment: 130 Effective length of query: 366 Effective length of database: 404,274,708 Effective search space: 147964543128 Effective search space used: 147964543128 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)