BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0251.0020.p (619 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_242976.1| BH2110~unknown conserved protein [Bacillus halo... 177 5e-43 ref|NP_623881.1| predicted ATPase [Thermoanaerobacter tengcongen... 176 2e-42 ref|NP_244314.1| two-component sensor histidine kinase [Bacillus... 161 4e-38 ref|NP_782429.1| two-component sensor kinase yesM [Clostridium t... 156 1e-36 ref|NP_345167.1| sensor histidine kinase, putative [Streptococcu... 151 5e-35 >ref|NP_242976.1| BH2110~unknown conserved protein [Bacillus halodurans] pir||F83913 hypothetical protein BH2110 [imported] - Bacillus halodurans (strain C-125) dbj|BAB05829.1| BH2110~unknown conserved protein [Bacillus halodurans] Length = 585 Score = 177 bits (450), Expect = 5e-43, Method: Compositional matrix adjust. Identities = 170/616 (27%), Positives = 291/616 (47%), Gaps = 70/616 (11%) Query: 14 IRNMKLQNKLMLGYVLVCVIPLLSVSAFIFQQSAKGLEDSSQEFASLYTSQIRTTLNEFL 73 + N+KL+NKL+L Y+ +P+L + + +A ++ + L QI N + Sbjct: 8 LNNVKLRNKLLLLYIFCVFLPILLTNIIFYNVTANNIKAQKMDDIELVVDQITNEFNNAV 67 Query: 74 KEYEEVTKSVLVDNDIIYSLGEAENLPFDKLIDQRLAVQRLLMRVALLKSEMSSVMLVS- 132 + ++ S N IY E E ++ ID A L + S+ +S Sbjct: 68 DQAIGIS-SGFYTNTRIYDFFETE---YETAIDYIEAYDFYLREYSRFSPLYYSIQSISF 123 Query: 133 -RDDSVYQFSTSTNKVNENALLAQEWYTGLRDSNETFFITGLHDRSYYEDKGEGAVVTAG 191 D+ + ++ + E+ ++WY L ++N I + D G +V+ Sbjct: 124 YTDNPSFIYAGGIRPLTEHTK-EEKWYRLLTEANHPIIIRT----NPATDSGLFSVI--- 175 Query: 192 RVLFSSDGAY--AGMLLIDLDPFTLLQLDHEFILARDKYGISVVITNRQQQIVYHSDAAS 249 R L Y ++ IDL+P T+ Q I + V + N ++ I + S+ Sbjct: 176 RELDYHKNKYNHKKIVRIDLNPLTIKQ-----IFNNAAFTGEVYLLNGERNIEFSSNE-- 228 Query: 250 GQLSWT------QVLESGEEYIQDKG---DKDHI---VLLGSTSQGELFIRTDIPRSRLL 297 +++W LE E+ I K ++++ + G+ S+ E I ++ +SR Sbjct: 229 -EVNWRTEQLHFDALEFPEDTIFTKERMLSQNYLEGWRVAGTISENE--ILEELYKSREF 285 Query: 298 QKINQIKVMTVIVILMSCLIITLFSFGLSFTITRPIKALRRSMKQAEVGQYLAIEKEQAN 357 I ++ I +L L+I L S + + R +K ++R KQ ++ I Sbjct: 286 -----IVILASINLLFPTLLIVLISKSIHSRLLRVLKHMKRMKKQ----KFDIINFPGDK 336 Query: 358 DEIGSLVHSYNKMITTIRTLIEDVYIGEI-------KQRQAKFIALQNQINPHMLYNTLE 410 DEIG L + +NKM TI LI +VY I +++QA+ ALQ+QINPH L+N LE Sbjct: 337 DEIGELTNEFNKMSQTINRLINEVYASNIEKKDLQLREKQAQLSALQSQINPHFLFNALE 396 Query: 411 SIRMKALIKDDYETADMIKILARMFRLVLNKEGKQHSVKHELEYTANYLQLQNIRFDNMF 470 +IRM+++IK + ETA +I+ +A++FR L +V+ EL +L++Q RF + Sbjct: 397 TIRMRSMIKGEKETAKIIQNMAKIFRNSLTWGKDWVTVRDELCLIICFLEIQKYRFGDKM 456 Query: 471 KLEIKIPGEMLECSIIPLVFQPIVENSINHGFENYSR--IMDIVIEGNWTEQGEMLFRIT 528 + E+ I E + I +VF P VEN+ HG E+ I+ I +E N GE+++ I+ Sbjct: 457 EYELDIDEEAYDYIIPNMVFLPFVENASIHGIESVKEKGIIQIRVEKN---DGEIVYEIS 513 Query: 529 DNGVGMSLDKQSELRALLDGADSHKYKLESNGGSTEKGLGLQNIAERIKLHYGDRYYLTI 588 DNG GM +K + L L K E N G + +G++N+ R+KLHY + + +I Sbjct: 514 DNGAGMDEEKLASLIETL--------KNEENMG---ENVGIKNVYYRLKLHYKNHFDFSI 562 Query: 589 VPMGAQGVTIEILIPG 604 QG T++I +P Sbjct: 563 NSRPGQGTTVQIKLPA 578 >ref|NP_623881.1| predicted ATPase [Thermoanaerobacter tengcongensis] gb|AAM25485.1| predicted ATPase [Thermoanaerobacter tengcongensis] Length = 593 Score = 176 bits (446), Expect = 2e-42, Method: Compositional matrix adjust. Identities = 126/356 (35%), Positives = 190/356 (53%), Gaps = 31/356 (8%) Query: 263 EYIQDKGDKDHIVLLGSTSQGELFIRTDIPRS--------RLLQKINQIKVMTV---IVI 311 +Y++DK + V++ S L + IP S L IN I++ + + I Sbjct: 250 KYLKDKQN----VVININSSKLLVTSSPIPTSDWRLVSVVSLKNAINSIRISFIEFYLRI 305 Query: 312 LMSCLIIT-LFSFGLSFTITRPIKALRRSMKQAEVGQYL-AIEKE-QANDEIGSLVHSYN 368 L+ CL+ L S S +T P++ L SMK+ G +E E + DEI ++ YN Sbjct: 306 LVFCLLFAFLVSMYFSRALTHPLERLANSMKKVRQGNLEEKVEFEYHSKDEIAIVIKEYN 365 Query: 369 KMITTIRTLIEDVYIGEIKQRQAKFIALQNQINPHMLYNTLESIRMKALIKDDYETADMI 428 +M+ I LI + EI +R+A+ AL+ QINPH LYNTL++I KA D E ++MI Sbjct: 366 RMVERINDLINRILKLEINKREAELKALEAQINPHFLYNTLDTIFWKAEKAKDRELSEMI 425 Query: 429 KILARMFRLVLNKEGKQHSVKHELEYTANYLQLQNIRFDNMFKLEIKIPGEMLECSIIPL 488 L+R+FRL LN+ + V+ E E YL LQ+ R+ N FK +KI E+L+ I L Sbjct: 426 YSLSRLFRLTLNRGEEFIQVRGEKELIERYLFLQSKRYKNKFKYSVKISDEILDYYIPKL 485 Query: 489 VFQPIVENSINHGFENYSRIMDIVIEGNWTEQGEML-FRITDNGVGMSLDKQSELRALLD 547 + QP VEN+I HG EN S + +E ++GE L F I DNG GMS + +L+ Sbjct: 486 ILQPFVENAIVHGLENNSE--ESFVEVIGEKEGENLKFIIKDNGPGMSEETLEKLKRF-- 541 Query: 548 GADSHKYKLESNGGSTEKGLGLQNIAERIKLHYGDRYYLTIVPMGAQGVTIEILIP 603 +ES + G ++N+ ER++L+YGD+Y L I +G TI +++P Sbjct: 542 --------IESENIEEKVGYAIRNVNERLRLYYGDKYELKIESRQGEGTTISLILP 589 >ref|NP_244314.1| two-component sensor histidine kinase [Bacillus halodurans] pir||G84080 two-component sensor histidine kinase BH3447 [imported] - Bacillus halodurans (strain C-125) dbj|BAB07166.1| two-component sensor histidine kinase [Bacillus halodurans] Length = 602 Score = 161 bits (408), Expect = 4e-38, Method: Compositional matrix adjust. Identities = 100/286 (34%), Positives = 156/286 (54%), Gaps = 11/286 (3%) Query: 322 SFGLSFTITRPIKALRRSMKQAEVGQYLAIEKEQANDEIGSLVHSYNKMITTIRTLIEDV 381 S+ LS+TI+RPI L +M++AE G + + + DE+G L S+N MI I LI Sbjct: 313 SYFLSYTISRPINQLMLAMQKAESGDLTSRYEGEEQDEVGRLGRSFNTMIEQIGKLISLT 372 Query: 382 YIGEIKQRQAKFIALQNQINPHMLYNTLESIRMKALIKDDYETADMIKILARMFRLVLNK 441 E K+R+A+ +LQ I PH LYNTL++I+ A K+ + AD++ L+++FR+ L+K Sbjct: 373 ERQERKKREAELHSLQANIKPHFLYNTLDTIQWMARKKNASDVADLVGALSKLFRIGLSK 432 Query: 442 EGKQHSVKHELEYTANYLQLQNIRFDNMFKLEIKIPGEMLECSIIPLVFQPIVENSINHG 501 G + E+E+ NYLQ+Q++R+ N + +P M SI+ +V QPIVEN+I HG Sbjct: 433 GGTIIPLVDEIEHIKNYLQIQSVRYKNKLNYSLVMPSNMERLSILKIVLQPIVENAIYHG 492 Query: 502 FENYSRIMDIVIEGNWTEQGEMLFRITDNGVGMSLDKQSELRA----LLDGADSHKYKLE 557 + I IE E ++ RI DNG G+ + +LR L+ D + Sbjct: 493 IKERRGPGLITIEAE-RENDMLIIRIRDNGKGIKPETLQQLRTNVNLFLEKGD------K 545 Query: 558 SNGGSTEKGLGLQNIAERIKLHYGDRYYLTIVPMGAQGVTIEILIP 603 G KG GL N+ R++L +G+ Y + I + +G + I P Sbjct: 546 EGGQRLTKGYGLINVHARLRLTFGEPYGVKIDSVFNEGTVVTIFHP 591 >ref|NP_782429.1| two-component sensor kinase yesM [Clostridium tetani E88] gb|AAO36366.1| two-component sensor kinase yesM [Clostridium tetani E88] Length = 577 Score = 156 bits (395), Expect = 1e-36, Method: Compositional matrix adjust. Identities = 113/357 (31%), Positives = 193/357 (54%), Gaps = 26/357 (7%) Query: 260 SGEEYIQDKGDKDHIVLLGSTSQGELFIRTDIPRSRLLQ-KINQIKVMTVIVILMSCLII 318 SG +++ +KD V S E +I I ++ + +INQI + + V L+ L Sbjct: 236 SGTVALKNVHNKDSTVFFSSVPYTENWILCIIVENKKIHTQINQIINLILFVGLILLLAA 295 Query: 319 TLFSFGLSFTITRPIKALRRSMKQAEVGQYLAIEKEQANDEIGSLVHSYNKMITTIRTLI 378 L S +S +I +PI L+ +M + G + + + + NDEI L +N+M+T I+ LI Sbjct: 296 ILLSIVISGSIVKPIHRLKSNMLEISKGNFDSYYEIKNNDEISILGQVFNQMLTDIKRLI 355 Query: 379 EDVYIGEIKQRQAKFIALQNQINPHMLYNTLESIRMKALIKDDYETADMIKILARMFRLV 438 VY E ++R A+ LQ+QINPH LYNTL++I+ KAL + +E DMI L+ FR+ Sbjct: 356 NKVYQVEKQKRSAELRVLQSQINPHFLYNTLDTIQWKALEYNAFEVVDMINYLSNFFRIS 415 Query: 439 LNKEGKQH-SVKHELEYTANYLQLQNIRFDNMFKLEIKIPGEMLECSIIPLVFQPIVENS 497 L+ +GK+ ++ E+ + +NYL++Q IR+ + + + + + I L+ QP+VENS Sbjct: 416 LS-DGKEFITISDEVAHVSNYLEIQKIRYKDKISYSMDVDSSLDQYLIPKLIVQPLVENS 474 Query: 498 INHG--FENYSRIMDIVIEGNWTEQGEMLFRITDNGVGMSLDKQSELRA-LLDGADSHKY 554 I HG + + I+++ I ++ G + ++ DNG+GM +++ LR L + +++ Y Sbjct: 475 IYHGLKLQKHKGIINVSIS---SKDGFLFIKVIDNGLGMDVEQLRTLRKNLWESIETNHY 531 Query: 555 KLESNGGSTEKGLGLQNIAERIKLHYGDRYYLTIVPMGAQGVTIEILIP----GFDA 607 GL NI ER+KL +G +Y + I G + + IP GF+ Sbjct: 532 -------------GLYNINERLKLAFGKKYSIEIKSELKIGTQVLLRIPLISEGFEC 575 >ref|NP_345167.1| sensor histidine kinase, putative [Streptococcus pneumoniae TIGR4] pir||F95076 sensor histidine kinase, probable [imported] - Streptococcus pneumoniae (strain TIGR4) emb|CAB54581.1| histidine kinase [Streptococcus pneumoniae] gb|AAF31453.1| putative histidine kinase [Streptococcus pneumoniae] gb|AAK74807.1| sensor histidine kinase, putative [Streptococcus pneumoniae TIGR4] Length = 563 Score = 151 bits (381), Expect = 5e-35, Method: Compositional matrix adjust. Identities = 149/600 (24%), Positives = 258/600 (43%), Gaps = 58/600 (9%) Query: 22 KLMLGYVLVCVIPLLSVSAFIFQQSAKGLEDSSQEFASLYTSQIRTTLNEFLKEYEEVTK 81 ++++ LV +I L V F +Q S+ +E + + + SQ + ++K+ E + Sbjct: 9 RMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGNSQTTISQTSHFIQSYIKKLETTST 68 Query: 82 SVLVDNDIIYSLGEAENLPFDKLIDQRLAVQRLLMRVALLKSEMSSVMLVSRDDSVYQFS 141 + D+ L AEN DK+ ++ L + + ++ +V+LV++ V Sbjct: 69 GLTQQTDV---LAYAENPSQDKV----EGIRDLFLTILKSDKDLKTVVLVTKSGQVISTD 121 Query: 142 TSTNKVNENALLAQEWYTGLRDSNETFFITGLHDRSYYEDKGEGAVVTAGRVLFSSDGAY 201 S + ++A++WY +T V++ + L + GA Sbjct: 122 DSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSD------SQWVISVTQELVDAKGAN 175 Query: 202 AGMLLIDLDPFTLLQLDHEFILARDKYGISVVITNRQQQIVYHS-----DAASGQLSWTQ 256 G+L +D+ TL ++ L + + I N + VYH ++S + Sbjct: 176 LGVLRLDISYETLEAYLNQLQLGQQGFAF---IINENHEFVYHPQHTVYSSSSKMEAMKP 232 Query: 257 VLESGEEYI--------QDKGDKDHIVLLGSTSQGELFIRTDIPRSRLLQKINQIKVMTV 308 +++G+ Y Q+K +LG +S +L D RS+LL + V ++ Sbjct: 233 YIDTGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKL----DQVRSQLLWTLLGASVTSL 288 Query: 309 IVILMSCLIITLFSFGLSFTITRPIKALRRSMKQAEVGQYLAIEKEQANDEIGSLVHSYN 368 +V CL + FS P+K LR +M + G KE E+ + +N Sbjct: 289 LV----CLCLVWFSLKRWIA---PLKDLRETMLEIASGAQNLRAKEVGAYELREVTRQFN 341 Query: 369 KMITTIRTLIEDVYIGEIKQRQAKFIALQNQINPHMLYNTLESIRMKALIKDDYETADMI 428 M+ I L+ + E RQ + AL +QINPH LYNTL++I A D + Sbjct: 342 AMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFHDSQRVVQVT 401 Query: 429 KILARMFRLVLNKEGKQHSVKHELEYTANYLQLQNIRFDNMFKLEIKIPGEMLECSIIPL 488 K LA FRL LN+ + E+ + YL +Q R+ + + EI + L Sbjct: 402 KSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINENVAFDNLVLPKL 461 Query: 489 VFQPIVENSINHGFENYSRIMDIVIEGNWTEQGEMLFRITDNGVGMSLDKQSELRALLDG 548 V QP+VEN++ HG + I + + G ++ RI D+GVG D Sbjct: 462 VLQPLVENALYHGIKEKEGQGHIKLSVQKQDSG-LVIRIEDDGVGFQ-----------DA 509 Query: 549 ADSHKYKLESNGGSTEKGLGLQNIAERIKLHYGDRYYLTIVPMGAQGVTIEILIPGFDAS 608 DS + +L+ G+GLQN+ +R+KLH+G Y++ I +G +EI I + S Sbjct: 510 GDSSQSQLKRG------GVGLQNVDQRLKLHFGANYHMKIDSRPQKGTKVEIYINRIETS 563 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.320 0.136 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 838,644,965 Number of extensions: 34128954 Number of successful extensions: 94677 Number of sequences better than 1.0e-05: 179 Number of HSP's gapped: 94445 Number of HSP's successfully gapped: 179 Length of query: 619 Length of database: 757,237,578 Length adjustment: 132 Effective length of query: 487 Effective length of database: 398,844,510 Effective search space: 194237276370 Effective search space used: 194237276370 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)