BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0251.0019.p (241 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value pir||JC5346 cdd2 protein - Clostridium difficile >gi|1770138|emb... 202 4e-51 ref|ZP_00099616.1| COG4200: Uncharacterized protein conserved in... 159 4e-38 ref|NP_721536.1| putative transporter, trans-membrane domain bac... 107 2e-22 emb|CAB60257.1| MrsE protein [Bacillus sp. HIL-Y85/54728] 96 5e-19 ref|NP_940777.1| NukG [Staphylococcus warneri] >gi|37572674|dbj|... 96 1e-18 >pir||JC5346 cdd2 protein - Clostridium difficile emb|CAA63567.1| cdd2 [Clostridium difficile] Length = 245 Score = 202 bits (515), Expect = 4e-51, Method: Compositional matrix adjust. Identities = 115/240 (47%), Positives = 155/240 (64%), Gaps = 2/240 (0%) Query: 3 RSVAAEWPKLRHSRMGLVLASLPVISLLIGCFNFYFNQEALQNGWYSLWTQVSLFYGEFF 62 +SV E KL++S + VL +LP+IS+LIG NFY NQ L+ WYSLWTQVSLFYGEFF Sbjct: 4 KSVLGEILKLKNSFIWYVLLALPLISVLIGSGNFYLNQGILKKEWYSLWTQVSLFYGEFF 63 Query: 63 LPILIAICCAFVCRLEHVNRNWNMILAAPVPITSIFLAKLAIVGILIAGAQVSFLILYWT 122 PILIAI CA++CRLEH+N NWN ++ PV + +IFL+KL +V IL A QV F+I Y Sbjct: 64 FPILIAIFCAYICRLEHMNHNWNNVMTLPVNLKNIFLSKLTVVSILTAITQVFFVIFYIV 123 Query: 123 AGQMLSIMEPFPMEIILWTFRGWVASITIAALQLGLSIRIRSFATPIGISLC-AVF-IXX 180 AG M P E+ W +GW+ SI+IA++QL +SI+IRSFATPIGISLC ++F + Sbjct: 124 AGNMFGFSGSVPKELTGWIAKGWIISISIASIQLYMSIKIRSFATPIGISLCLSLFGMGL 183 Query: 181 XXXXXXXXXXFPYSLLTIGMGVLTQRGLTLAENLLFFIMNGVYIVLLSVWSIRRLRYRDV 240 FPYS+L MGV+ Q L + + ++ + + ++ S + R R DV Sbjct: 184 IVAKPAVGIFFPYSMLGSRMGVIKQSALNVNDTIVTICVAFITTIVFSEIANRNFRKNDV 243 >ref|ZP_00099616.1| COG4200: Uncharacterized protein conserved in bacteria [Desulfitobacterium hafniense] Length = 244 Score = 159 bits (403), Expect = 4e-38, Method: Compositional matrix adjust. Identities = 93/239 (38%), Positives = 140/239 (58%), Gaps = 2/239 (0%) Query: 3 RSVAAEWPKLRHSRMGLVLASLPVISLLIGCFNFYFNQEALQNGWYSLWTQVSLFYGEFF 62 R++ AE KLRHS + L LP++ LIG FN+ N LQ+GWYSLWTQ +LF FF Sbjct: 4 RALRAEGMKLRHSPVWLAFILLPILPALIGTFNYLLNIGILQDGWYSLWTQHTLFSCYFF 63 Query: 63 LPILIAICCAFVCRLEHVNRNWNMILAAPVPITSIFLAKLAIVGILIAGAQVSFLILYWT 122 LP +I + C+++ RLEH+N NWN ++ P+P++ FLAKL I ++ QV LY Sbjct: 64 LPAIIGVYCSYLYRLEHLNHNWNSVMTMPIPVSCFFLAKLCIASAMVIFTQVWTGALYLL 123 Query: 123 AGQMLSIMEPFPMEIILWTFRGWVASITIAALQLGLSIRIRSFATPIGISLCAVFIXXXX 182 +G++ + P P + W G A+I I A+QL LS+ IRSFA P+G++L Sbjct: 124 SGKLAGLSSPVPGQFPEWLLTGAAAAIAICAVQLCLSLMIRSFAVPVGLALMGGIAGLGM 183 Query: 183 XXXXXXXXFPYSLLTIGMGVLTQRGLTLAENLLFFIMN-GVYIVLLSVWSIRRLRYRDV 240 FPYSLL++GM Q G + +L F+++ +Y++ +W++ ++ RDV Sbjct: 184 LAKGYGVFFPYSLLSLGMRA-NQPGGPMQCDLGHFLLSCALYVLACVLWAVLWMKRRDV 241 >ref|NP_721536.1| putative transporter, trans-membrane domain bacteriocin immunity protein [Streptococcus mutans UA159] gb|AAN58842.1| putative transporter, trans-membrane domain bacteriocin immunity protein [Streptococcus mutans UA159] Length = 242 Score = 107 bits (267), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 68/236 (28%), Positives = 120/236 (50%), Gaps = 5/236 (2%) Query: 8 EWPKLRHSRMGLVLASLPVISLLIGCFNFYFNQEALQNGWYS--LWTQVSLFYGEFFLPI 65 E+ K+R S + ++L ++ +G F N+ L +G + LW Q + + F PI Sbjct: 5 EFKKIRKSAIPIILIFFNLVGTSLGTMMFALNRNYLVDGTEASILWGQTVFYASQIFTPI 64 Query: 66 LIAICCAFVCRLEHVNRNWNMILAAPVPITSIFLAKLAIVGILIAGAQVSFLILYWTAGQ 125 LI C+ C+ E N+NW +L P+ I L K+ + +++A +Q+ L Y + Sbjct: 65 LIGTICSISCQFEENNKNWQRLLTIPIKPNRIILTKITSLSVVMAISQLLLLFFYVLSAV 124 Query: 126 MLSI-MEPFPMEIILWTFRGWVASITIAALQLGLSIRIRSFATPIGIS--LCAVFIXXXX 182 +L + F + +LW+ GW+A+ITI +Q+ ++IR+++FA PI IS L + Sbjct: 125 LLKVSFVGFLPDFLLWSITGWLATITIVTIQIFINIRLKNFAVPILISAILAMAGLMTLF 184 Query: 183 XXXXXXXXFPYSLLTIGMGVLTQRGLTLAENLLFFIMNGVYIVLLSVWSIRRLRYR 238 FPY+ + +G+ TL E LF +N +YI+L ++ +L+ R Sbjct: 185 IGNSVFTIFPYAQVAVGLRARALVPFTLPELSLFLGLNVIYIILFYGLAVSQLKKR 240 >emb|CAB60257.1| MrsE protein [Bacillus sp. HIL-Y85/54728] Length = 244 Score = 96.3 bits (238), Expect = 5e-19, Method: Compositional matrix adjust. Identities = 70/235 (29%), Positives = 112/235 (47%), Gaps = 7/235 (2%) Query: 11 KLRHSRMGLVLASLPVISLLIGCFNFYFNQEAL----QNGWYSLWTQVSLFYGEFFLPIL 66 KL+ S++ ++ P++ + + NF N N W WTQV+LF G F LPI+ Sbjct: 11 KLKRSKLWIIYLLGPLLGVSLAYTNFIKNYNLFMNPGDNPWVEAWTQVALFMGPFVLPIV 70 Query: 67 IAICCAFVCRLEHVNRNWNMILAAPVPITSIFLAKLAIVGILIAGAQVSFLILYWTAGQM 126 + I A VCR EHV W +LA PV + IFL K V +I + ++L+ G M Sbjct: 71 VGIFAALVCRGEHVGGGWKQLLALPVKHSDIFLGKFLTVVRMIFISMSILILLFIGFGYM 130 Query: 127 LSIMEPFPMEIIL-WTFRGWVASITIAALQLGLSIRIRSFATPIGISLCAVFIXXXXXXX 185 L I P+ IL + RG +A + + LQL +SIR ++F P+ +S+ Sbjct: 131 LGISGSLPLLTILGYGIRGILACLPLILLQLIVSIRSKTFGIPLAVSIVFTLPAIIIAST 190 Query: 186 XXXXXFPYSLLTIGMGVLTQRGLTLAENLLFFIMNGVYIVLLSVWSIRRLRYRDV 240 +P++ + M + + N LF+ + + + L V+ IR RD+ Sbjct: 191 PLGQVYPWTQPMLAMSPEDES--PIQSNFLFYSIMVITCLGLLVYGIRSFTKRDL 243 >ref|NP_940777.1| NukG [Staphylococcus warneri] dbj|BAC98764.1| NukG [Staphylococcus warneri] dbj|BAD01012.1| NukG [Staphylococcus warneri] Length = 245 Score = 95.5 bits (236), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 69/232 (29%), Positives = 116/232 (50%), Gaps = 15/232 (6%) Query: 5 VAAEWPKLRHSRMGLVLASLPVISLLIGCFNFYFNQEALQNGWYS---LWTQVSLFYGEF 61 ++ E KL+++ + + L ++S+ IG FYFNQ A N LW Q +L+ + Sbjct: 5 LSIELRKLQNNYILIFLLVFNILSVSIGSMIFYFNQSAFPNSNNRDLILWGQETLYTSQL 64 Query: 62 FLPILIAICCAFVCRLEHVNRNWNMILAAPVPITSIFLAKLAIVGILIAGAQVSFLILYW 121 F PILI + C+ + E N NW + P+ + I L+K + +L Q+ F +L+ Sbjct: 65 FFPILIGVLCSISWQFEESNNNWMRMKTIPLKESKIVLSKFLSLFLLTLLNQIIFFVLFC 124 Query: 122 TAGQMLSIMEPFPMEIILWTFRGWVASITIAALQLGLSIRIRSFATPIGIS-------LC 174 + ++++ ++ + W F GW+ + +I A+QL LS+ R+F I IS L Sbjct: 125 ISASIVNVSLEGLLKFLYWDFIGWIGTASIVAIQLYLSVATRNFTFSILISTIGGVLGLL 184 Query: 175 AVFIXXXXXXXXXXXXFPYSLLTIGMGVLTQRGLTLAENLLFFIMNGVYIVL 226 +FI FPYS +T+G+ + T +E LLF I+N YI + Sbjct: 185 TLFI-----SNFIFKIFPYSQITVGVRARSLMNFTFSEMLLFLIINVAYITV 231 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.333 0.145 0.467 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 319,073,053 Number of extensions: 11715732 Number of successful extensions: 50985 Number of sequences better than 1.0e-05: 15 Number of HSP's gapped: 50965 Number of HSP's successfully gapped: 15 Length of query: 241 Length of database: 757,237,578 Length adjustment: 122 Effective length of query: 119 Effective length of database: 425,995,500 Effective search space: 50693464500 Effective search space used: 50693464500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 39 (21.5 bits) S2: 124 (52.4 bits)