BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0251.0018.p (249 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value pir||JC5347 cdd3 protein - Clostridium difficile >gi|1770139|emb... 174 2e-42 gb|AAC72254.1| LctE [Lactococcus lactis subsp. lactis] 89 1e-16 ref|ZP_00099615.1| COG1277: ABC-type transport system involved i... 77 4e-13 emb|CAB60256.1| MrsG protein [Bacillus sp. HIL-Y85/54728] 72 1e-11 gb|AAD16031.1| membrane spanning protein MutE [Streptococcus mut... 69 1e-10 >pir||JC5347 cdd3 protein - Clostridium difficile emb|CAA63568.1| cdd3 [Clostridium difficile] Length = 253 Score = 174 bits (440), Expect = 2e-42, Method: Compositional matrix adjust. Identities = 84/218 (38%), Positives = 139/218 (63%), Gaps = 3/218 (1%) Query: 1 MKALSLEYYKIRRRKVWAMLTLFLAAELGWASMSMGISISRSA---ANATWEALIFSLSS 57 MK L LE+YK++R+K+ M LF+A E+ W ++ +I+R+ + ++ S +S Sbjct: 1 MKYLGLEFYKLKRKKIILMTFLFVAVEIMWCIVNSNRAITRNPDMLGGFEYSYMLMSFAS 60 Query: 58 MNGLFLPILSAIVVSRICDMEHKGDTWKMLLTTSVGRSSIYAAKYICAGSLMLYGILAQA 117 +NGLF PIL +I+ SRI D+EHKGDTWK+L ++ ++IY +K++C+ L+ +L Q Sbjct: 61 LNGLFFPILISIITSRISDIEHKGDTWKLLKSSVTSLNTIYLSKFLCSAILVSIPVLMQV 120 Query: 118 VFIVGFGVLHKLDGPLPVALLLQFAAGTLSANLLITAMQQWISLAVKNQAFALCLGMLGA 177 + IV FG + + V+LLL++ GT+ ++ I A+ WI+ + NQ A+ GM+G+ Sbjct: 121 LSIVLFGHFRGVIESISVSLLLKYTLGTILVSIAIIALHLWIATVIPNQMIAITFGMIGS 180 Query: 178 FLGTTASLFPAAVRHVIVWSYYLDLSPVTYVYKASSGT 215 F+G T+ LF +R + +WSYYL+LSP++Y Y G+ Sbjct: 181 FIGLTSGLFYQGIRRLFIWSYYLELSPLSYAYDNVLGS 218 >gb|AAC72254.1| LctE [Lactococcus lactis subsp. lactis] Length = 250 Score = 88.6 bits (218), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 56/217 (25%), Positives = 112/217 (51%), Gaps = 3/217 (1%) Query: 1 MKALSLEYYKIRRRKVWAMLTLFLAAELGWASMSMGISISRSAANATWEALIFSLSSMNG 60 +K + LE K +RR+ ++ L +F L W+S+ + LI L+ +N Sbjct: 2 LKYIYLELLKTKRRRFFSPLIIFFVVGLLWSSVLFVKEYPLPYSGDKIVILINDLTIINS 61 Query: 61 LFLPILSAIVVSRICDMEHKGDTWKMLLTTSVGRSSIYAAKYICAGSLMLYGILAQAVFI 120 +F P++ I+ S + +EH T+K+L T +++ AK I A ++L L Q +I Sbjct: 62 MFSPLIIGIMCSNLFSLEHDNQTFKVLQTNGQSIENLFYAKTIFAFLIILIFGLFQFFYI 121 Query: 121 VGFGVLHKLDGPLPVALLLQFAAGTLSANLLITAMQQWISLAVKNQAFALCLGMLGAFLG 180 +L K+ P+ ++ L + G + A+L + + +ISL ++ Q+ + + ++ +F+G Sbjct: 122 NLIAILFKIGDPIYLSFL--YFIGYIIASLFLIILHLFISLKIRKQSLGIIISVIASFVG 179 Query: 181 -TTASLFPAAVRHVIVWSYYLDLSPVTYVYKASSGTY 216 + + P ++ +I W Y+ LSP+ +K++S Y Sbjct: 180 LVSGGMLPLYLKFIIPWQYFSLLSPIHREFKSNSFLY 216 >ref|ZP_00099615.1| COG1277: ABC-type transport system involved in multi-copper enzyme maturation, permease component [Desulfitobacterium hafniense] Length = 250 Score = 76.6 bits (187), Expect = 4e-13, Method: Compositional matrix adjust. Identities = 62/201 (30%), Positives = 98/201 (48%), Gaps = 8/201 (3%) Query: 2 KALSLEYYKIRRRKVWAMLTLFLAAELGWASMSMGISISRSAANATWEALIFSLSSMNGL 61 KALS+E+ K R RK+W ++ L +L W + + W ++ +N + Sbjct: 3 KALSVEFRKTRHRKIWLIVAALLGFQLLWFFWGLK-HMDAGKLQQGWLLCLYQFPVLNAI 61 Query: 62 FLPILSAIVVSRICDMEHKGDTWKMLLTTSVGRSSIYAAKYICAGSLMLYGILAQ--AVF 119 LP+++A+V SR+ D+EHKG T++ LL T + ++ AK++C +L ++ Q A+ Sbjct: 62 ILPVVTAVVASRLSDVEHKGQTFR-LLNTVMPAGKLFDAKFLCGAFYILAIVILQVMAML 120 Query: 120 IVGFGVLHKLDGPLP-VALLLQFAAGTLSANLLITAMQQWISLAVKNQAFALCLGMLGAF 178 + G+ PLP + L F + LLI Q +SL NQ L LG+LG F Sbjct: 121 LAGYAKGFTESPPLPQLGYYLLFTSWVTLTLLLI---QHIVSLLSTNQMIPLSLGLLGGF 177 Query: 179 LGTTASLFPAAVRHVIVWSYY 199 G P +I W YY Sbjct: 178 AGLFIMYLPPQFARLIPWGYY 198 >emb|CAB60256.1| MrsG protein [Bacillus sp. HIL-Y85/54728] Length = 247 Score = 72.0 bits (175), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 50/155 (32%), Positives = 82/155 (52%), Gaps = 12/155 (7%) Query: 44 ANATWEALIFSLSSMNGLFLPILSAIVVSRICDMEHKGDTWKMLLTTSVGRSSIYAAKYI 103 A + W L++ S + GL P+ + + S I ++EH+ + WK L+ V R IY +K+I Sbjct: 47 AGSMWMYLLYDNSLLFGLGFPLAATLSASIIANIEHQANGWKQTLSFPVSRMRIYVSKFI 106 Query: 104 CAGSLMLYGILAQAVFIVGFGVLHKL---DGPLPVALLLQFAAGTLSANLLITAMQQWIS 160 C L++ ++ +F++G +L KL +G +P LL + L L I A Q W+S Sbjct: 107 C---LVVSLFISSTIFLLGMVLLGKLVGFEGSVPWGLLFGDSYSMLVTVLPIMAFQIWLS 163 Query: 161 LAVKNQAFALCLG----MLGAFLGTTASL--FPAA 189 + NQAF++ +G ++G FL S FP A Sbjct: 164 MVFSNQAFSILVGSVSSIMGLFLAAAQSTRWFPLA 198 >gb|AAD16031.1| membrane spanning protein MutE [Streptococcus mutans] Length = 246 Score = 68.9 bits (167), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 55/229 (24%), Positives = 114/229 (49%), Gaps = 19/229 (8%) Query: 1 MKALSLEYYKIRRRKVW-AMLTLFLAAELGWASMSMGISISRSAANATWEALIFSLSSMN 59 M+ L E K RR ++W +L LFL + + W S+S + I++++++ ++ LIF+L ++N Sbjct: 1 MRQLRSEIQKYRRNRLWLPILGLFLFS-IYWCSVSNNLQINKNSSD-IFQILIFNLYTIN 58 Query: 60 GLFLPILSAIVVSRICDMEHKGDTWKMLLTTSVGRSSIYAAKYICAGSLMLYGILAQAVF 119 L +P +A++ SR+ ++ K + +L T ++ +KY+ ++ G + Q +F Sbjct: 59 NLIIPFSAALLASRMVMIDKKNRMYSVLFTNGENGIGLFLSKYVLGLLILFIGFVCQLIF 118 Query: 120 IV-----GFGVLHKLDGPLPVALLLQFAAGTLSANLLITAMQQWISLAVKNQAFALCLGM 174 +++K + L + +L +GT I +Q +S +++ + +G+ Sbjct: 119 TFISSYQYHAIINKNEFILFIITML-LGSGT------ILLIQLLLSFVIQSSIIPIVIGL 171 Query: 175 LGAFLGT-TASLFPAAVRHVIVWSYYLDLSPVTYVYKASSGTYITGTMP 222 G+FL T+ + + I W Y L+P T SG +++ P Sbjct: 172 AGSFLSMLTSGVLAKQITIFIPWEYLSTLNPYTI---NDSGIHLSANYP 217 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.325 0.135 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 303,266,381 Number of extensions: 10347033 Number of successful extensions: 36226 Number of sequences better than 1.0e-05: 15 Number of HSP's gapped: 36233 Number of HSP's successfully gapped: 15 Length of query: 249 Length of database: 757,237,578 Length adjustment: 123 Effective length of query: 126 Effective length of database: 423,280,401 Effective search space: 53333330526 Effective search space used: 53333330526 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 124 (52.4 bits)