BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0251.0017.p (342 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value pir||JC5348 cdd4 protein - Clostridium difficile >gi|1770140|emb... 322 9e-87 ref|ZP_00099614.1| COG1131: ABC-type multidrug transport system,... 306 4e-82 ref|NP_940775.1| NukF [Staphylococcus warneri] >gi|37572672|dbj|... 282 8e-75 emb|CAB60255.1| MrsF protein [Bacillus sp. HIL-Y85/54728] 278 1e-73 emb|CAB37661.1| cdd4-like protein [Bacillus sphaericus] 271 2e-71 >pir||JC5348 cdd4 protein - Clostridium difficile emb|CAA63569.1| cdd4 [Clostridium difficile] Length = 301 Score = 322 bits (825), Expect = 9e-87, Method: Compositional matrix adjust. Identities = 164/294 (55%), Positives = 208/294 (70%), Gaps = 1/294 (0%) Query: 43 IIETQHLTKSYGGTPRVDHINLAVQQGEIFGFLGPNGAXXXXXXXXXXXXIQPTKGSVKL 102 II TQ L+K Y RV I+L V QG ++GFLGPNGA +PT+G++ + Sbjct: 4 IIRTQRLSKEYNKIFRVKDIDLRVPQGAVYGFLGPNGAGKSTTLKMLLGLAKPTQGNINI 63 Query: 103 FGKEL-RNNRESILNQTGSLIESPSYYGHLTGLENLRVMQRLRSLPAKNIDKVLQIVRLD 161 GKEL NR SIL GSLIESPSYYGHLTGLEN+ +MQRL LP KN+++ L+IVRL+ Sbjct: 64 LGKELNEKNRISILKDIGSLIESPSYYGHLTGLENMTIMQRLLDLPKKNVNEALKIVRLE 123 Query: 162 NHKHKPARQYSLGMKQRLGLAMALLAFPSLLILDEPTNGLDPAGIGEIRELIQSLPAQYG 221 N K+K QYSLGMKQRLG+AMA++ FP LLILDEPTNGLDPAGI EIRELI+SLP +YG Sbjct: 124 NQKNKKVSQYSLGMKQRLGIAMAIMKFPKLLILDEPTNGLDPAGIEEIRELIKSLPEKYG 183 Query: 222 MTILVSSHLLSEIEQIATSVGIISEGKLLFQGTMAALQAQNQAAIHFQTHDPAMAVKVLA 281 MT+L+SSHLLSEI+QIATS+GII+ G+L+FQ ++ L + Q+ I +T + A K+L Sbjct: 184 MTVLISSHLLSEIDQIATSIGIINHGELVFQDSIKELHNRGQSQIAIKTKNVVSAQKLLN 243 Query: 282 AHGYRPRIQERLLVFGAMQDSEVARINEILXXXXXXXXXXEAYKKSLEDIFLDL 335 GY IQ+ L ++DS+VA+IN +L E +KKSLEDIFL+L Sbjct: 244 TKGYMSLIQDGYLTIENLKDSDVAKINGMLVHSNIDVFRIEEHKKSLEDIFLEL 297 >ref|ZP_00099614.1| COG1131: ABC-type multidrug transport system, ATPase component [Desulfitobacterium hafniense] Length = 304 Score = 306 bits (784), Expect = 4e-82, Method: Compositional matrix adjust. Identities = 160/301 (53%), Positives = 208/301 (69%), Gaps = 1/301 (0%) Query: 43 IIETQHLTKSYGGTPRVDHINLAVQQGEIFGFLGPNGAXXXXXXXXXXXXIQPTKGSVKL 102 II+TQ L K YG TP V ++L+V +G ++GFLGPNGA ++PT GS+ + Sbjct: 4 IIKTQGLCKHYGKTPGVSDLDLSVAEGAVYGFLGPNGAGKSTTLKMILGLVKPTAGSITV 63 Query: 103 FGKELRN-NRESILNQTGSLIESPSYYGHLTGLENLRVMQRLRSLPAKNIDKVLQIVRLD 161 FGK + + +R SIL GSLIESPSYYGHL+G ENLR++ L+ P K+I +VL+IVRL+ Sbjct: 64 FGKTMNHRSRMSILKDVGSLIESPSYYGHLSGYENLRIIATLKGTPEKDIGRVLEIVRLE 123 Query: 162 NHKHKPARQYSLGMKQRLGLAMALLAFPSLLILDEPTNGLDPAGIGEIRELIQSLPAQYG 221 N K+K QYSLGMKQRLGLA ALL P LLILDEPTNGLDPAGI E+RELI+SLP +YG Sbjct: 124 NQKNKKTNQYSLGMKQRLGLACALLGNPRLLILDEPTNGLDPAGIQEMRELIRSLPKEYG 183 Query: 222 MTILVSSHLLSEIEQIATSVGIISEGKLLFQGTMAALQAQNQAAIHFQTHDPAMAVKVLA 281 +T+LVSSHLLSEIEQ+AT++GIIS+G+L+FQ ++ AL ++ +I +T D A VL Sbjct: 184 ITVLVSSHLLSEIEQMATNLGIISKGRLVFQSSLGALHQKSSPSIAIRTLDNHRAAAVLR 243 Query: 282 AHGYRPRIQERLLVFGAMQDSEVARINEILXXXXXXXXXXEAYKKSLEDIFLDLTGKERS 341 G + Q+ L+ A D E++R+ L E +KSLEDIFL+LTG S Sbjct: 244 EQGIAVQCQQDYLLLPAFADGELSRLTLNLFEQKVGVVRIEQREKSLEDIFLELTGSAVS 303 Query: 342 L 342 L Sbjct: 304 L 304 >ref|NP_940775.1| NukF [Staphylococcus warneri] dbj|BAC98762.1| NukF [Staphylococcus warneri] dbj|BAD01010.1| NukF [Staphylococcus warneri] Length = 291 Score = 282 bits (722), Expect = 8e-75, Method: Compositional matrix adjust. Identities = 139/288 (48%), Positives = 201/288 (69%), Gaps = 3/288 (1%) Query: 43 IIETQHLTKSYGGTPRVDHINLAVQQGEIFGFLGPNGAXXXXXXXXXXXXIQPTKGSVKL 102 I++TQ+LTK + + VD+++L + EI+GFLGPNGA +QPTKG++K+ Sbjct: 5 IVQTQNLTKKFSDSYSVDNLSLNIGSKEIYGFLGPNGAGKSTTMKMLLGLMQPTKGNIKI 64 Query: 103 FGKELRNNRESILNQTGSLIESPSYYGHLTGLENLRVMQRLRSLPAKNIDKVLQIVRLDN 162 F +++ NR+ IL G+LIE PSYY +LTGLENL+V+QRL +LP+KN+ + L+IVRL Sbjct: 65 FNQDISKNRDEILMHVGALIEEPSYYKNLTGLENLQVIQRLLNLPSKNVKEALKIVRLTE 124 Query: 163 HKHKPARQYSLGMKQRLGLAMALLAFPSLLILDEPTNGLDPAGIGEIRELIQSLPAQYGM 222 HK K + YSLGMKQRLG+A+A++ FP LLILDEPTNGLDP+GI EIRELI+S P YGM Sbjct: 125 HKDKLVKNYSLGMKQRLGIALAIVKFPKLLILDEPTNGLDPSGIQEIRELIKSFPKTYGM 184 Query: 223 TILVSSHLLSEIEQIATSVGIISEGKLLFQGTMAALQAQNQAAIHFQTHDPAMAVKVLAA 282 T+L+SSHLLSEIE +A +VGII+ GKLLF+G + L+ QN+ I+ T++ + ++ +L + Sbjct: 185 TVLISSHLLSEIEHMANTVGIINRGKLLFEGKLTELEEQNKILIN--TNNNSESINLLQS 242 Query: 283 HGYRPRIQERLLVFGAMQDSEVARINEILXXXXXXXXXXEAYKKSLED 330 GY E+ L+ Q +++ ++L ++ +KSLE+ Sbjct: 243 KGYNLENNEKPLLLDTTQ-KDISTAVKLLVNNKFEIYQVQSVQKSLEE 289 >emb|CAB60255.1| MrsF protein [Bacillus sp. HIL-Y85/54728] Length = 303 Score = 278 bits (712), Expect = 1e-73, Method: Compositional matrix adjust. Identities = 145/300 (48%), Positives = 200/300 (66%), Gaps = 1/300 (0%) Query: 43 IIETQHLTKSYGGTPRVDHINLAVQQGEIFGFLGPNGAXXXXXXXXXXXXIQPTKGSVKL 102 I+ T LTK Y VD +NL +++GEI+GFLGPNGA I+PTKG++++ Sbjct: 5 IVVTHDLTKKYKKHTSVDGLNLRIRRGEIYGFLGPNGAGKTTTIRMLLGLIKPTKGNIEI 64 Query: 103 FGKELRNNRESILNQTGSLIESPSYYGHLTGLENLRVMQRLRSLPAKNIDKVLQIVRLDN 162 FG+ L NR IL + GSL+ESP+YYG+LTG ENL ++RLR LP + +++VL+ VRL Sbjct: 65 FGQNLNKNRLQILQRIGSLVESPTYYGNLTGYENLEAVRRLRGLPEQQVNEVLETVRLSK 124 Query: 163 HKHKPARQYSLGMKQRLGLAMALLAFPSLLILDEPTNGLDPAGIGEIRELIQSLPAQYGM 222 ++ ++YSLGMKQRLG+A+ALL+ P LLILDEPTNGLDP+GI EIRELI+ LP + GM Sbjct: 125 VANRLTKEYSLGMKQRLGIAVALLSSPDLLILDEPTNGLDPSGIQEIRELIKELP-KSGM 183 Query: 223 TILVSSHLLSEIEQIATSVGIISEGKLLFQGTMAALQAQNQAAIHFQTHDPAMAVKVLAA 282 +++VSSHLLSEI+Q+AT VGII+ GK++FQ ++A+L + + + D A +L Sbjct: 184 SVIVSSHLLSEIDQMATQVGIINNGKMIFQDSIASLHQKRKPLLKVGVSDVIEAKTILNR 243 Query: 283 HGYRPRIQERLLVFGAMQDSEVARINEILXXXXXXXXXXEAYKKSLEDIFLDLTGKERSL 342 G + +Q+ L + V+ IN IL E +SLEDIFL+LTG E SL Sbjct: 244 KGLKVDLQKNYLWLSQTEPEFVSEINSILLHSGLSVFRLEEKTRSLEDIFLELTGTEGSL 303 >emb|CAB37661.1| cdd4-like protein [Bacillus sphaericus] Length = 311 Score = 271 bits (693), Expect = 2e-71, Method: Compositional matrix adjust. Identities = 136/300 (45%), Positives = 196/300 (65%) Query: 43 IIETQHLTKSYGGTPRVDHINLAVQQGEIFGFLGPNGAXXXXXXXXXXXXIQPTKGSVKL 102 I++TQ+L+K++G V +INL +++GEI+GFLGPNGA ++P+ G++K+ Sbjct: 12 IVQTQNLSKNFGKEQAVSNINLQIRKGEIYGFLGPNGAGKTTTIRMLLGLMKPSSGTIKI 71 Query: 103 FGKELRNNRESILNQTGSLIESPSYYGHLTGLENLRVMQRLRSLPAKNIDKVLQIVRLDN 162 F K++ +R IL GSL+E+PSYY HLT ENL ++++ +P ID+VLQ VRL Sbjct: 72 FQKDIVKDRIQILANVGSLVENPSYYPHLTAYENLEALRKILGVPKTRIDEVLQTVRLKE 131 Query: 163 HKHKPARQYSLGMKQRLGLAMALLAFPSLLILDEPTNGLDPAGIGEIRELIQSLPAQYGM 222 +K + +SLGMKQRLG+A ALL P LLILDEPTNGLDP+GI EIR LI+ LP +YGM Sbjct: 132 AANKKVKGFSLGMKQRLGIAAALLHNPELLILDEPTNGLDPSGIIEIRNLIKRLPKEYGM 191 Query: 223 TILVSSHLLSEIEQIATSVGIISEGKLLFQGTMAALQAQNQAAIHFQTHDPAMAVKVLAA 282 TI++SSHLLSEI+Q+AT VGI+S+G L+FQ ++ ++ Q + F+ + + L A Sbjct: 192 TIIISSHLLSEIDQMATQVGIVSKGTLIFQDSIEGMRKFAQPKVTFKVSNGEKGWRSLLA 251 Query: 283 HGYRPRIQERLLVFGAMQDSEVARINEILXXXXXXXXXXEAYKKSLEDIFLDLTGKERSL 342 +G + Q+ ++ D +VA I ++L E K+SLEDIFL +T E L Sbjct: 252 NGIKANYQDDVIFLEECSDEKVAHIVQVLVQEGISVFRVEEEKRSLEDIFLQMTAGELKL 311 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.135 0.376 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 440,625,477 Number of extensions: 16328127 Number of successful extensions: 87671 Number of sequences better than 1.0e-05: 14728 Number of HSP's gapped: 77491 Number of HSP's successfully gapped: 15380 Length of query: 342 Length of database: 757,237,578 Length adjustment: 126 Effective length of query: 216 Effective length of database: 415,135,104 Effective search space: 89669182464 Effective search space used: 89669182464 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 126 (53.1 bits)