BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0251.0015.p (482 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00099612.1| COG0642: Signal transduction histidine kinase... 258 3e-67 emb|CAB93670.1| RumK protein [Ruminococcus gnavus] 237 4e-61 ref|NP_721531.1| putative histidine kinase; homolog of RumK and ... 130 8e-29 pir||T09002 histidine kinase homolog scnK - Streptococcus pyogen... 124 7e-27 ref|NP_655026.1| HATPase_c, Histidine kinase-like ATPases [Bacil... 115 2e-24 >ref|ZP_00099612.1| COG0642: Signal transduction histidine kinase [Desulfitobacterium hafniense] Length = 454 Score = 258 bits (658), Expect = 3e-67, Method: Compositional matrix adjust. Identities = 160/459 (34%), Positives = 231/459 (50%), Gaps = 10/459 (2%) Query: 1 MDSAVRILRKFVGXXXXXXXXXXXXXXXXXXXXIFKETHQETSPETVVREIAAGLKTAAG 60 M S +RIL ++VG + QE V IA GL Sbjct: 1 MKSMLRILGRYVGSAAGIALILLILNAALFLLWV-TGAKQENGNSYRVSVIADGLAKEGE 59 Query: 61 KHALEPEXXXXXXXXXXXXXXXNQEGKVQWSEQLPAEIPRSYSILEVAKFSRYYLQGYPV 120 ++ L + G++ WS LP E+ R+Y+I EVA F+++YLQ YPV Sbjct: 60 EYTLADSARQAMSVRYEWAMLLSDSGEILWSLNLPRELSRTYTIPEVASFTKWYLQDYPV 119 Query: 121 YIWEHPEGLLVVGYPKHSYEKYQLEYLTDWXXXXXXXXXXXXXXXXXXXXXXXXFIGTRL 180 Y+W H +GLLVVG PK S K +E +G RL Sbjct: 120 YVWRHQDGLLVVGCPKGSVWKTGMEMPQSIMDKTILWIPIVLAANGLAAVLLALLLGLRL 179 Query: 181 IRGIRPLIDGIHALAKDKPVRVETGGIFSDLSESINLASATLQAKNTALKSRDEARSNWV 240 R + L+ GI +A +PV++ T G+ +L+ ++N S LQ + AL+ R++AR++W+ Sbjct: 180 FRSMHTLVKGIEQMAAKQPVQLGTNGVLGELAAALNQTSRELQRQEIALQKREQARTSWI 239 Query: 241 AGISHDIRTPLSMILGYASNLEEQVNLTXXXXXXXXXXXXXXXXLRSLVSDLNLVSMLEY 300 AGISHDIRTPLSM++GYAS LE+ L +++LV+DLNL S LEY Sbjct: 240 AGISHDIRTPLSMVMGYASQLEDNQELPRPAREQAGIVRQQSQRIKTLVNDLNLASKLEY 299 Query: 301 DMQPLQLKPVRLSTLARTVASDIINNGLDERYPIDLSITDESLQ--VMGDEKLLYRAVSN 358 DMQPL + V L+ L R +A+D +N GL Y ++L + + + Q V+GD++LL RAV N Sbjct: 300 DMQPLHQEEVLLAPLLRQIAADFLNGGLARPYSLELDVGESARQAKVLGDQELLRRAVYN 359 Query: 359 LVQNSVQHNPDGCRIVLRSSRGPEDCCYSLSVSDTGAGIAPEHLPELVLLPYSAGRVHPV 418 L+QNS++HNP GC I + R + C+ ++V+D G G E L L G Sbjct: 360 LIQNSIRHNPQGCAIRITLER--VNSCWVMAVADNGKGFDQELLDRLKEENSLTG----- 412 Query: 419 RQGHGLGLPMVARIAEAHKGKLILESGTAQGLHATLQFP 457 HGLGL +V +IA H+G E+ G + P Sbjct: 413 MPSHGLGLTLVRQIARVHEGTTEFENLPEGGCRVVINLP 451 >emb|CAB93670.1| RumK protein [Ruminococcus gnavus] Length = 459 Score = 237 bits (605), Expect = 4e-61, Method: Compositional matrix adjust. Identities = 132/379 (34%), Positives = 202/379 (53%), Gaps = 13/379 (3%) Query: 83 NQEGKVQWSEQLPAEIPRSYSILEVAKFSRYYLQGYPVYIWEHPEGLLVVGYPKHSYEKY 142 N++G V WS P ++P +Y I +VA F++ Y+Q YPV+ WE G++V+GYP+ SY K Sbjct: 82 NEDGVVTWSHNKPKKVPNTYKIKDVAVFAKGYIQDYPVFTWECEGGIMVLGYPEDSYTKI 141 Query: 143 QLEYLTDWXXXXXXXXXXXXXXXXXXXXXXXXFIGTR-LIRGIRPLIDGIHALAKDKPVR 201 Y + F+ R +I+ P+I I L+ K V Sbjct: 142 TSNYFSLDAIRKLPIFILIMFCLDIFLLFIAYFLSKRKIIKTTEPIIAAIKNLSDGKSVS 201 Query: 202 VETGGIFSDLSESINLASATLQAKNTALKSRDEARSNWVAGISHDIRTPLSMILGYASNL 261 + G +++E +N A+ L +N +AR+NW++G+SHDIRTPLSMI+GYA + Sbjct: 202 ISVDGELVEIAEGVNRAAIILDRQN-------QARANWISGVSHDIRTPLSMIMGYAQRI 254 Query: 262 EEQVNLTXXXXXXXXXXXXXXXXLRSLVSDLNLVSMLEYDMQPLQLKPVRLSTLARTVAS 321 ++ ++ LV DLNLVS LEY MQPL +R S L R+ + Sbjct: 255 SDETEGESLIHNEAVIIRKQSMKIKELVQDLNLVSQLEYSMQPLNKTKIRFSALLRSYVA 314 Query: 322 DIINNGLDERYPIDLSITD--ESLQVMGDEKLLYRAVSNLVQNSVQHNPDGCRIVLRSSR 379 +++N G+ E+Y I+L ++ E + DE+L+ RA+SNLVQNS++HNP GC IV+R Sbjct: 315 ELLNEGIPEKYSINLEVSSFVEQFWIDCDERLITRAISNLVQNSIRHNPKGCEIVIRLDY 374 Query: 380 GPEDCCYSLSVSDTGAGIAPEHLPELVLLP-YSAGRVHPVRQGHGLGLPMVARIAEAHKG 438 + L + D G G++ + L EL +P Y + HGLGL +V +I AH G Sbjct: 375 VEHG--FELQIKDNGIGLSEKRLRELQEIPHYMESTDERLDLRHGLGLIIVRQIVIAHGG 432 Query: 439 KLILESGTAQGLHATLQFP 457 K+ ++S +G T+ P Sbjct: 433 KIKIDSEPNRGYTTTIFLP 451 >ref|NP_721531.1| putative histidine kinase; homolog of RumK and ScnK [Streptococcus mutans UA159] gb|AAN58837.1| putative histidine kinase; homolog of RumK and ScnK [Streptococcus mutans UA159] Length = 437 Score = 130 bits (326), Expect = 8e-29, Method: Compositional matrix adjust. Identities = 98/384 (25%), Positives = 171/384 (44%), Gaps = 40/384 (10%) Query: 83 NQEGKVQWSEQLPAEIPRSYSILEVAKFSRYYLQGYPVYIWEHPEGLLVVGYP-KHSYEK 141 NQ+G++ S +LP ++ R Y+ ++ KFSR+YL YPV+ + + +LV GYP K+S K Sbjct: 82 NQDGQIVQSYRLPQQLKRHYTNADMVKFSRWYLDDYPVFSYVRGQQILVFGYPKKYSLAK 141 Query: 142 YQLEYLTDWXXXXXXXXXXXXXXXXXXXXXXXXFIGTRLIRGIRPLIDGIHALAKDKPVR 201 + T + R+ + I P++ + L++ + Sbjct: 142 FNFYLNTQFIKNFLHLGFVFLGIIFLFIFVLFSHSKLRIKKEIEPIVSALTFLSQGQSAA 201 Query: 202 VETGGIFSDLSESINLASATLQAKNTALKSRDEARSNWVAGISHDIRTPLSMILGYASNL 261 + G FS++ ++N S L+ N + W+ G+SHD+R PL +I GY S L Sbjct: 202 LNEKGNFSEIKTALNETSHILEESNM-------MKEQWIRGVSHDLRNPLMLISGYTSQL 254 Query: 262 EEQVNLTXXXXXXXXXXXXXXXXLRSLVSDLNLVSMLEYDMQPLQLKPVRLSTLARTVAS 321 E+Q ++ ++S+LNL +L+ + + + L+ + R+V + Sbjct: 255 EQQYG----HGKQTEQIENQIQNMKEMISNLNLSYLLDNQKRHSKFSSLDLAVVLRSVIA 310 Query: 322 DIINNGLDERYPIDLSITDESLQVMGDEKLLYRAVSNLVQNSVQHNPDGCRIVLRSSRGP 381 DI+NN E + + E++ V GD LL RA NL+ NS++H S Sbjct: 311 DILNNY--ETVILHFDLPTEAVLVKGDATLLERAFRNLLLNSIKH----------SGSNQ 358 Query: 382 EDCCYSLS-------VSDTGAGIAPEHLPELVLLPYSAGRVHPVRQGHGLGLPMVARIAE 434 D Y L ++D G I + + EL + + HG+G + +I + Sbjct: 359 IDLTYQLFDNKVIIIIADKGT-ITAQKIVEL-------NQKSTNYESHGMGTVITKQIIK 410 Query: 435 AHKGKLILESGTAQGLHATLQFPA 458 H+G+ + S GL T+ P Sbjct: 411 LHQGETVF-SDNHPGLKVTIVLPV 433 >pir||T09002 histidine kinase homolog scnK - Streptococcus pyogenes gb|AAB92598.1| ScnK [Streptococcus pyogenes] Length = 453 Score = 124 bits (310), Expect = 7e-27, Method: Compositional matrix adjust. Identities = 98/377 (25%), Positives = 169/377 (44%), Gaps = 15/377 (3%) Query: 85 EGKVQWSEQLPAEIPRSYSILEVAKFSRYYLQGYPVYIWEHPEGLLVVGYPKHSYEKYQL 144 GK +++ P I + + +FSR+YL+ YPV+ + ++ +PK S +Y Sbjct: 82 NGKEKFNINKPQIIENQFDFADAIRFSRFYLKDYPVFTQIKDNDIYIIAFPKDSIIRYSS 141 Query: 145 EYL-TDWXXXXXXXXXXXXXXXXXXXXXXXXFIGTRLIRGIRPLIDGIHALAKDKPVRVE 203 Y + + T L R I+P+I+ I L +V+ Sbjct: 142 NYFELNRVQIFPILILSIIFANCLFCLFLYIYSVTFLNRNIKPIINAITKLPNGINTQVK 201 Query: 204 TGGIFSDLSESINLASATLQAKNTALKSRDEARSNWVAGISHDIRTPLSMILGYASNLEE 263 + L+ +IN A N L+ +E + NW++GI+HDI+TPLS+I+ S E Sbjct: 202 SVRELDKLTLAINSA-------NQQLRENEEFKENWISGIAHDIKTPLSVIVSNTSLAIE 254 Query: 264 QVNLTXXXXXXXXXXXXXXXXLRSLVSDLNLVSMLEYDMQPLQLKPVRLSTLARTVASDI 323 + N +++L++DLN+ + L L + V + + + I Sbjct: 255 KTN-DDEFLKHLKPTLVESHYIQNLLNDLNIFARLTNGNLKLNHEIVDIIPFFKEIIIQI 313 Query: 324 INNGLDERYPIDLSITDESL---QVMGDEKLLYRAVSNLVQNSVQHNPDGCRIVLRSSRG 380 IN + E + + I D L ++ ++ L+ R V NL+ NSV HN GC I + Sbjct: 314 INQEIWEDFNFEF-IPDSKLLGKKMYIEKALISRVVHNLIYNSVLHNHSGCDIKIILEYL 372 Query: 381 PEDCCYSLSVSDTGAGIAPEHLPELVLLPYSAGRVHPVRQGHGLGLPMVARIAEAHKGKL 440 PED C+S+++ D G G +PE L + + + VR+ G+GL + +I + H G + Sbjct: 373 PED-CFSITIRDNGIGTSPERLKNINKIEEFNFDMSGVRRS-GMGLKISKQIIDLHDGNM 430 Query: 441 ILESGTAQGLHATLQFP 457 I+ S Q + P Sbjct: 431 IITSTQGQYFQTVITLP 447 >ref|NP_655026.1| HATPase_c, Histidine kinase-like ATPases [Bacillus anthracis A2012] ref|NP_843604.1| sensor histidine kinase [Bacillus anthracis str. Ames] gb|AAP25090.1| sensor histidine kinase [Bacillus anthracis str. Ames] Length = 580 Score = 115 bits (288), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 76/272 (27%), Positives = 129/272 (47%), Gaps = 13/272 (4%) Query: 196 KDKPVRVETGG-------IFSDLSESINLASATLQAKNTALKSRDEARSNWVAGISHDIR 248 K KP+R G +FSD++ S+ S TL+ + + + R W+ G++HD++ Sbjct: 309 KGKPIRFRKSGKEKWSFRLFSDVTNSLEHLSITLKKNDAMRQVLQQTREEWITGLTHDLK 368 Query: 249 TPLSMILGYASNLE-EQVNLTXXXXXXX-XXXXXXXXXLRSLVSDLNLVSMLEYDMQPLQ 306 TPLS I GYA LE +Q N T + +L+ DL+L L+ + P Q Sbjct: 369 TPLSSIYGYALLLESKQYNWTDRDIQQFGSVMKEKSQYMTTLIDDLSLTYQLKNNSLPAQ 428 Query: 307 LKPVRLSTLARTVASDIINNGLDERYPIDLSITDESLQVMGDEKLLYRAVSNLVQNSVQH 366 + ++ + V INN + I+ + +Q +EK R + NL+ N+V+H Sbjct: 429 HVNIEMNQFVQKVLLQFINNPTLQNQNIEFVPSSNKIQYFIEEKWFQRIIENLLVNAVKH 488 Query: 367 NPDGCRIVLRSSRGPEDCCYSLSVSDTGAGIAPEHLPELVLLPYSAG-RVHPVRQGHGLG 425 N + ++++ S+ ++LS+SD G G+ + EL+ Y G G GLG Sbjct: 489 NNETTNVIVKLSQNA--TSFTLSISDNGKGM-DDKTKELLFERYYRGTNTEESNIGTGLG 545 Query: 426 LPMVARIAEAHKGKLILESGTAQGLHATLQFP 457 L + ++ AH G + ++S +G L FP Sbjct: 546 LAITKQLVHAHNGAISIDSELGKGTTIILVFP 577 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.316 0.135 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 590,824,743 Number of extensions: 21505308 Number of successful extensions: 65594 Number of sequences better than 1.0e-05: 2278 Number of HSP's gapped: 64278 Number of HSP's successfully gapped: 2285 Length of query: 482 Length of database: 757,237,578 Length adjustment: 130 Effective length of query: 352 Effective length of database: 404,274,708 Effective search space: 142304697216 Effective search space used: 142304697216 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 128 (53.9 bits)