BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0251.0010.p (318 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value emb|CAB61230.1| vans protein [Bacillus circulans] 271 1e-71 ref|NP_878014.1| sensor histidine kinase VanS [Staphylococcus au... 267 2e-70 pir||B41838 two-component sensor histidine kinase homolog VanS [... 267 2e-70 ref|ZP_00098026.1| COG0642: Signal transduction histidine kinase... 262 6e-69 gb|AAR37057.1| VanSD5 [Enterococcus faecium] 183 5e-45 >emb|CAB61230.1| vans protein [Bacillus circulans] Length = 382 Score = 271 bits (694), Expect = 1e-71, Method: Compositional matrix adjust. Identities = 143/292 (48%), Positives = 192/292 (65%), Gaps = 1/292 (0%) Query: 2 NYAKLRMKVFVRMXXXXXXXXXXXXXXYSLVRGRLGEAGVRLLQYVFGYEYDHALSIYES 61 +Y+KL K+++ + S++RG+LGE VR L+ +G A+ +Y Sbjct: 9 DYSKLERKLYMYIVAIVVVAVIFVLYIRSMIRGKLGEWIVRFLENEYGLNQLDAMKLYHY 68 Query: 62 VLRDHWEXXXXXXXXXXXXXXXXXXXXWFTKYFNEVNAGIDALITDE-ADIKLSPEMSSM 120 +R++ E F KYF+E+N GID LI +E I+LS EM M Sbjct: 69 SIRNNIEIFIYVAIAISILILCRVMLSKFAKYFDEINTGIDVLIQNEDKQIELSAEMDVM 128 Query: 121 EQKLVFVKQTLQKRELEVQLSEQNKRDLVMYLAHDIKTPLTSVIGYLSLLHEAPDMPNEQ 180 E KL +K+TL+KRE + +L+EQ K D+VMYLAHDIKTPLTS+IGYLSLL EAPDMP +Q Sbjct: 129 EHKLNTLKRTLEKREQDAKLAEQRKNDVVMYLAHDIKTPLTSIIGYLSLLDEAPDMPVDQ 188 Query: 181 KENYTKITLEKAYHLERLINDFFEVERYNQQTIQLKKQNVDLSYMLIQMPDEFYPSLTPA 240 K Y ITL+KAY LE+LI++FFE+ RYN QTI L K ++DL YML+QM DEFYP L+ Sbjct: 189 KAKYVHITLDKAYRLEQLIDEFFEITRYNLQTITLTKTHIDLYYMLVQMTDEFYPQLSAH 248 Query: 241 GKRMIVNVEDTITVYGDSEKLARVFNNILKNAIAYSDDNSTIEVSAKVQKDM 292 GK+ I++ +TV GD +KLARVFNNILKNA AYS+DNS I+++A + +M Sbjct: 249 GKQAIIHASVDLTVSGDPDKLARVFNNILKNAAAYSEDNSIIDITAGLSGNM 300 >ref|NP_878014.1| sensor histidine kinase VanS [Staphylococcus aureus] sp|Q06240|VANS_ENTFC Sensor protein vanS (Vancomycin resistance protein vanS) (Vancomycin histidine protein kinase) gb|AAA24788.1| vancomycin histidine protein kinase gb|AAA65954.1| vanS [Enterococcus faecium] gb|AAQ17158.1| sensor histidine kinase VanS [Staphylococcus aureus] Length = 384 Score = 267 bits (683), Expect = 2e-70, Method: Compositional matrix adjust. Identities = 138/294 (46%), Positives = 195/294 (66%), Gaps = 1/294 (0%) Query: 2 NYAKLRMKVFVRMXXXXXXXXXXXXXXYSLVRGRLGEAGVRLLQYVFGYEYDHALSIYES 61 +Y+KL K+++ + S++RG+LG+ + +L+ + + A+ +Y+ Sbjct: 11 DYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGKLGDWILSILENKYDLNHLDAMKLYQY 70 Query: 62 VLRDHWEXXXXXXXXXXXXXXXXXXXXWFTKYFNEVNAGIDALITDE-ADIKLSPEMSSM 120 +R++ + F KYF+E+N GID LI +E I+LS EM M Sbjct: 71 SIRNNIDIFIYVAIVISILILCRVMLSKFAKYFDEINTGIDVLIQNEDKQIELSAEMDVM 130 Query: 121 EQKLVFVKQTLQKRELEVQLSEQNKRDLVMYLAHDIKTPLTSVIGYLSLLHEAPDMPNEQ 180 EQKL +K+TL+KRE + +L+EQ K D+VMYLAHDIKTPLTS+IGYLSLL EAPDMP +Q Sbjct: 131 EQKLNTLKRTLEKREQDAKLAEQRKNDVVMYLAHDIKTPLTSIIGYLSLLDEAPDMPVDQ 190 Query: 181 KENYTKITLEKAYHLERLINDFFEVERYNQQTIQLKKQNVDLSYMLIQMPDEFYPSLTPA 240 K Y ITL+KAY LE+LI++FFE+ RYN QTI L K ++DL YML+QM DEFYP L+ Sbjct: 191 KAKYVHITLDKAYRLEQLIDEFFEITRYNLQTITLTKTHIDLYYMLVQMTDEFYPQLSAH 250 Query: 241 GKRMIVNVEDTITVYGDSEKLARVFNNILKNAIAYSDDNSTIEVSAKVQKDMAG 294 GK+ +++ + +TV GD +KLARVFNNILKNA AYS+DNS I+++A + D+ Sbjct: 251 GKQAVIHAPEDLTVSGDPDKLARVFNNILKNAAAYSEDNSIIDITAGLSGDVVS 304 >pir||B41838 two-component sensor histidine kinase homolog VanS [similarity] - Enterococcus faecium Length = 384 Score = 267 bits (683), Expect = 2e-70, Method: Compositional matrix adjust. Identities = 138/294 (46%), Positives = 195/294 (66%), Gaps = 1/294 (0%) Query: 2 NYAKLRMKVFVRMXXXXXXXXXXXXXXYSLVRGRLGEAGVRLLQYVFGYEYDHALSIYES 61 +Y+KL K+++ + S++RG+LG+ + +L+ + + A+ +Y+ Sbjct: 11 DYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGKLGDWILSILENKYDLNHLDAMKLYQY 70 Query: 62 VLRDHWEXXXXXXXXXXXXXXXXXXXXWFTKYFNEVNAGIDALITDE-ADIKLSPEMSSM 120 +R++ + F KYF+E+N GID LI +E I+LS EM M Sbjct: 71 SIRNNIDIFIYVAIVISILILCRVMLSKFAKYFDEINTGIDVLIQNEDKQIELSAEMDVM 130 Query: 121 EQKLVFVKQTLQKRELEVQLSEQNKRDLVMYLAHDIKTPLTSVIGYLSLLHEAPDMPNEQ 180 EQKL +K+TL+KRE + +L+EQ K D+VMYLAHDIKTPLTS+IGYLSLL EAPDMP +Q Sbjct: 131 EQKLNTLKRTLEKREQDAKLAEQRKNDVVMYLAHDIKTPLTSIIGYLSLLDEAPDMPVDQ 190 Query: 181 KENYTKITLEKAYHLERLINDFFEVERYNQQTIQLKKQNVDLSYMLIQMPDEFYPSLTPA 240 K Y ITL+KAY LE+LI++FFE+ RYN QTI L K ++DL YML+QM DEFYP L+ Sbjct: 191 KAKYVHITLDKAYRLEQLIDEFFEITRYNLQTITLTKTHIDLYYMLVQMTDEFYPQLSAH 250 Query: 241 GKRMIVNVEDTITVYGDSEKLARVFNNILKNAIAYSDDNSTIEVSAKVQKDMAG 294 GK+ +++ + +TV GD +KLARVFNNILKNA AYS+DNS I+++A + D+ Sbjct: 251 GKQAVIHAPEDLTVSGDPDKLARVFNNILKNAAAYSEDNSIIDITAGLSGDVVS 304 >ref|ZP_00098026.1| COG0642: Signal transduction histidine kinase [Desulfitobacterium hafniense] Length = 374 Score = 262 bits (670), Expect = 6e-69, Method: Compositional matrix adjust. Identities = 141/291 (48%), Positives = 187/291 (64%), Gaps = 3/291 (1%) Query: 2 NYAKLRMKVFVRMXXXXXXXXXXXXXXYSLVRGR--LGEAGVRLLQYVFGYEYDHALSIY 59 +Y KL+ ++F+RM +++ +G++ VR L F + A+ IY Sbjct: 8 DYTKLKRELFLRMLLIVFATAATVIFLRFVIQKNFSVGDSIVRFLMTAFDLRENVAVLIY 67 Query: 60 ESVLRDHWEXXXXXXXXXXXXXXXXXXXXWFTKYFNEVNAGIDALITDEA-DIKLSPEMS 118 SV + E WFTKYF+EV+AG+D L+ + A +I LSPE+ Sbjct: 68 RSVFYSNIEIITFIVILVFVVILLNLSTSWFTKYFDEVSAGMDKLVEESAAEIALSPELD 127 Query: 119 SMEQKLVFVKQTLQKRELEVQLSEQNKRDLVMYLAHDIKTPLTSVIGYLSLLHEAPDMPN 178 ME KL +K L+K++ +EQ K DLV+YLAHDIKTPLTSVIGYLSLL EAPDMP Sbjct: 128 FMENKLNQIKNNLEKQKKAALEAEQRKNDLVVYLAHDIKTPLTSVIGYLSLLDEAPDMPP 187 Query: 179 EQKENYTKITLEKAYHLERLINDFFEVERYNQQTIQLKKQNVDLSYMLIQMPDEFYPSLT 238 EQK Y ITLEKAY LE LIN+FFE+ R+N QTI L K+ ++L +ML Q+ DEFYP L Sbjct: 188 EQKAKYVGITLEKAYRLEELINEFFEITRFNLQTIVLNKEKINLQFMLQQLADEFYPMLA 247 Query: 239 PAGKRMIVNVEDTITVYGDSEKLARVFNNILKNAIAYSDDNSTIEVSAKVQ 289 P K++ VNV D +T++GD++KLARVFNNILKNAIAYS +NS I+++A Q Sbjct: 248 PQAKQVSVNVPDGLTLWGDADKLARVFNNILKNAIAYSYENSVIDIAAGQQ 298 >gb|AAR37057.1| VanSD5 [Enterococcus faecium] Length = 381 Score = 183 bits (464), Expect = 5e-45, Method: Compositional matrix adjust. Identities = 92/202 (45%), Positives = 137/202 (67%), Gaps = 1/202 (0%) Query: 91 TKYFNEVNAGIDALI-TDEADIKLSPEMSSMEQKLVFVKQTLQKRELEVQLSEQNKRDLV 149 +KYF E+N GID L+ D DI L PE++S E+K+ ++ TL KR+ + +L+EQ K DLV Sbjct: 102 SKYFKEINRGIDTLVHEDTNDIALPPELASTERKINSIRHTLTKRKTDAELAEQRKNDLV 161 Query: 150 MYLAHDIKTPLTSVIGYLSLLHEAPDMPNEQKENYTKITLEKAYHLERLINDFFEVERYN 209 MYLAHD+KTPL+SVIGYL+LL + + E +E Y I+L+KA LE LIN+FFE+ R+N Sbjct: 162 MYLAHDLKTPLSSVIGYLNLLRDENQISEELREKYLSISLDKAERLEELINEFFEITRFN 221 Query: 210 QQTIQLKKQNVDLSYMLIQMPDEFYPSLTPAGKRMIVNVEDTITVYGDSEKLARVFNNIL 269 I L ++L+ ML Q+ EF P L + +V+ + + D+ KL RVF+N+L Sbjct: 222 LSNITLVYSKINLTMMLEQLGHEFKPMLAGKNLKCEFDVQPDMMLSCDANKLQRVFDNVL 281 Query: 270 KNAIAYSDDNSTIEVSAKVQKD 291 +NA++Y +N+TI+V+A+ +D Sbjct: 282 RNAVSYCYENTTIQVNARQAED 303 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.134 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 385,870,489 Number of extensions: 13916267 Number of successful extensions: 52833 Number of sequences better than 1.0e-05: 1210 Number of HSP's gapped: 53594 Number of HSP's successfully gapped: 1210 Length of query: 318 Length of database: 757,237,578 Length adjustment: 125 Effective length of query: 193 Effective length of database: 417,850,203 Effective search space: 80645089179 Effective search space used: 80645089179 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 126 (53.1 bits)