BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0251.0009.p (231 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value emb|CAB61229.1| vanr protein [Bacillus circulans] 352 4e-96 ref|NP_878013.1| vancomycin response regulator VanR [Staphylococ... 347 1e-94 ref|ZP_00099216.1| COG0745: Response regulators consisting of a ... 302 5e-81 gb|AAQ16268.1| response regulator [Enterococcus faecalis] 282 5e-75 gb|AAR37056.1| VanRD5 [Enterococcus faecium] 278 9e-74 >emb|CAB61229.1| vanr protein [Bacillus circulans] Length = 231 Score = 352 bits (903), Expect = 4e-96, Method: Compositional matrix adjust. Identities = 158/231 (68%), Positives = 197/231 (85%) Query: 1 MVENILVVDDEQAITDLIEIYLKNENYKVTKFYNAQDALKYIESARVDLAILDIMLPDMN 60 M ILVVDDE I DL+E+YLKNENY V K+Y A++AL I+ +DLAILDIMLP + Sbjct: 1 MSNKILVVDDEHDIADLVELYLKNENYTVFKYYTAKEALDCIDKTELDLAILDIMLPGAS 60 Query: 61 GFALCRRIRENYHFPVIMLTAKDEETDKITGLTMGADDYMTKPFRPLEMVARVKAQLRRY 120 G ++C++IR+ Y +P+IMLTAKD E DKITGLT+GADDY+TKPFRPLE++ARVKAQLRRY Sbjct: 61 GLSICQKIRDKYTYPIIMLTAKDSEIDKITGLTIGADDYITKPFRPLELIARVKAQLRRY 120 Query: 121 KKYQTMPTPDEAVISYSGLVMDVNTHECWLNGQPLSLTPTEFSILRVLCENKGKVISSEQ 180 KKY + +E +I +SGLV+++NTHEC+LN +PLSLTPTEFSILR+LCENKG V+SSEQ Sbjct: 121 KKYSGVTEQNENIIVHSGLVININTHECFLNEKPLSLTPTEFSILRILCENKGNVVSSEQ 180 Query: 181 LFHAVWGNEYYNKNNNTITVHIRHLREKMNDSVDQPKWIRTIWGVGYKIEK 231 +FH +WG EY+NK+NNTITVHIRHLREKMN ++D PK+I+T+WGVGYKIEK Sbjct: 181 IFHEIWGEEYFNKSNNTITVHIRHLREKMNATIDNPKYIKTVWGVGYKIEK 231 >ref|NP_878013.1| vancomycin response regulator VanR [Staphylococcus aureus] sp|Q06239|VANR_ENTFC Regulatory protein vanR pir||A41838 transcription activator VanR - Enterococcus faecium gb|AAA24787.1| vancomycin response regulator gb|AAA24790.1| vanR gb|AAA65953.1| vanR [Enterococcus faecium] gb|AAM77882.1| cytoplasmic regulator [Enterococcus faecium] gb|AAQ17157.1| vancomycin response regulator VanR [Staphylococcus aureus] prf||1904198A vanR gene Length = 231 Score = 347 bits (890), Expect = 1e-94, Method: Compositional matrix adjust. Identities = 155/231 (67%), Positives = 198/231 (85%) Query: 1 MVENILVVDDEQAITDLIEIYLKNENYKVTKFYNAQDALKYIESARVDLAILDIMLPDMN 60 M + IL+VDDE I DL+E+YLKNENY V K+Y A++AL+ I+ + +DLAILDIMLP + Sbjct: 1 MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDKSEIDLAILDIMLPGTS 60 Query: 61 GFALCRRIRENYHFPVIMLTAKDEETDKITGLTMGADDYMTKPFRPLEMVARVKAQLRRY 120 G +C++IR+ + +P+IMLT KD E DKITGLT+GADDY+TKPFRPLE++ARVKAQLRRY Sbjct: 61 GLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITKPFRPLELIARVKAQLRRY 120 Query: 121 KKYQTMPTPDEAVISYSGLVMDVNTHECWLNGQPLSLTPTEFSILRVLCENKGKVISSEQ 180 KK+ + +E VI +SGLV++VNTHEC+LN + LSLTPTEFSILR+LCENKG V+SSE Sbjct: 121 KKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPTEFSILRILCENKGNVVSSEL 180 Query: 181 LFHAVWGNEYYNKNNNTITVHIRHLREKMNDSVDQPKWIRTIWGVGYKIEK 231 LFH +WG+EY++K+NNTITVHIRHLREKMND++D PK+I+T+WGVGYKIEK Sbjct: 181 LFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKYIKTVWGVGYKIEK 231 >ref|ZP_00099216.1| COG0745: Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain [Desulfitobacterium hafniense] Length = 227 Score = 302 bits (773), Expect = 5e-81, Method: Compositional matrix adjust. Identities = 137/205 (66%), Positives = 176/205 (85%), Gaps = 1/205 (0%) Query: 1 MVENILVVDDEQAITDLIEIYLKNENYKVTKFYNAQDALKYIESARVDLAILDIMLPDMN 60 + NIL+VDDEQAI DL+E+YLKNENY + KFYN +DAL I ++DLAILD+MLPD++ Sbjct: 23 LAANILIVDDEQAIADLVEVYLKNENYNLFKFYNGKDALDCIAKEKLDLAILDVMLPDVD 82 Query: 61 GFALCRRIRENYHFPVIMLTAKDEETDKITGLTMGADDYMTKPFRPLEMVARVKAQLRRY 120 GF++CR+IRE ++FPVIMLTAK+EE DKITGLT+GADDY+TKPFRPLE++ARVKAQLRR+ Sbjct: 83 GFSICRQIREKHNFPVIMLTAKEEEIDKITGLTLGADDYITKPFRPLELIARVKAQLRRF 142 Query: 121 KKYQTM-PTPDEAVISYSGLVMDVNTHECWLNGQPLSLTPTEFSILRVLCENKGKVISSE 179 KY + P +E VI++SGLV+D+NTHEC LN + LSLTPTEFSIL VLC N+G+V+SSE Sbjct: 143 TKYNSSEPNQEEHVIAFSGLVLDMNTHECTLNEKKLSLTPTEFSILWVLCSNRGRVVSSE 202 Query: 180 QLFHAVWGNEYYNKNNNTITVHIRH 204 +LF+ VWG++Y+ +NNT+ VHIRH Sbjct: 203 ELFNEVWGDKYFTNSNNTVMVHIRH 227 >gb|AAQ16268.1| response regulator [Enterococcus faecalis] Length = 235 Score = 282 bits (721), Expect = 5e-75, Method: Compositional matrix adjust. Identities = 138/236 (58%), Positives = 179/236 (75%), Gaps = 6/236 (2%) Query: 1 MVENILVVDDEQAITDLIEIYLKNENYKVTKFYNAQDALKYIESARVDLAILDIMLPDMN 60 M E IL+VDDE+ I DLIE+YLKN+ YKV KFYN DALK +ES ++DLAILD+MLPD++ Sbjct: 1 MNEKILIVDDEKEIADLIELYLKNDGYKVYKFYNGIDALKCVESEKMDLAILDVMLPDVD 60 Query: 61 GFALCRRIRENYHFPVIMLTAKDEETDKITGLTMGADDYMTKPFRPLEMVARVKAQLRRY 120 GF +C++IRE Y +P+IMLTAK E+ DKI GLT+GADDY+TKPF PLE+ ARVK QLRRY Sbjct: 61 GFHICQKIRERYFYPIIMLTAKVEDADKIMGLTIGADDYITKPFNPLEVAARVKTQLRRY 120 Query: 121 KKYQTMPTPDEA---VISY--SGLVMDVNTHECWLNGQPLSLTPTEFSILRVLCENKGKV 175 Y ++ V Y +GLV++ NTH+C L G+ ++LTP EFS+L LCEN+GKV Sbjct: 121 VCYNNAADIEKENVLVTEYDINGLVINKNTHKCTLYGKAVTLTPIEFSVLWYLCENRGKV 180 Query: 176 ISSEQLFHAVWGNEYYNKNNNTITVHIRHLREKMNDSVDQPKWIRTIWGVGYKIEK 231 ISSE+LF VWG ++ + NNNT+ HI LREK+ + PK+I+T+WGVGY IE+ Sbjct: 181 ISSEELFENVWGEKFLD-NNNTVMAHIGRLREKLKEPARNPKFIKTVWGVGYTIEE 235 >gb|AAR37056.1| VanRD5 [Enterococcus faecium] Length = 232 Score = 278 bits (710), Expect = 9e-74, Method: Compositional matrix adjust. Identities = 135/234 (57%), Positives = 177/234 (75%), Gaps = 5/234 (2%) Query: 1 MVENILVVDDEQAITDLIEIYLKNENYKVTKFYNAQDALKYIESARVDLAILDIMLPDMN 60 M E ILVVDDE+ + DL+E+YLKN+ Y V KFYN +DALK IES +DLAILDIMLPD++ Sbjct: 1 MNEKILVVDDEKELADLVEVYLKNDGYTVYKFYNGKDALKCIESVELDLAILDIMLPDVD 60 Query: 61 GFALCRRIRENYHFPVIMLTAKDEETDKITGLTMGADDYMTKPFRPLEMVARVKAQLRRY 120 GF +C++IRE ++FPVIMLTAK E+ DKI GL++ ADDY+TKPF PLE+VARVKAQLR+Y Sbjct: 61 GFQICQKIREKFYFPVIMLTAKVEDGDKIMGLSV-ADDYITKPFNPLEVVARVKAQLRQY 119 Query: 121 KKYQTMPTPDEAVIS---YSGLVMDVNTHECWLNGQPLSLTPTEFSILRVLCENKGKVIS 177 +Y+ EA + G+ + ++H+C L G+ + LTPTEFSIL LCE +G V+S Sbjct: 120 MRYKQPSLKQEAECTEYDIRGMTISKSSHKCILFGKEIQLTPTEFSILWYLCERQGTVVS 179 Query: 178 SEQLFHAVWGNEYYNKNNNTITVHIRHLREKMNDSVDQPKWIRTIWGVGYKIEK 231 +E+LF AVWG Y++ +NNT+ HI LREKM + PK+I+T+WGVGY IEK Sbjct: 180 TEELFEAVWGERYFD-SNNTVMAHIGRLREKMKEPSRNPKFIKTVWGVGYTIEK 232 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.320 0.136 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 358,636,674 Number of extensions: 14743695 Number of successful extensions: 56093 Number of sequences better than 1.0e-05: 5077 Number of HSP's gapped: 51499 Number of HSP's successfully gapped: 5117 Length of query: 231 Length of database: 757,237,578 Length adjustment: 122 Effective length of query: 109 Effective length of database: 425,995,500 Effective search space: 46433509500 Effective search space used: 46433509500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 124 (52.4 bits)